Restriction map for pF1KB6277
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BcgI        BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BseAI       BsiEI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NarI        NciI        NheI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflIII        448  ACACGT               A^C[GA][CT]GT
Alw44I       1625  GTGCAC               G^TGCAC
AocI          154  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1625  GTGCAC               G^TGCAC
BbrPI         449  CACGTG               CAC^GTG
BbsI         1372  GAAGAC               GAAGAC(2/6)
BbvCI        1897  GCTGAGG              CCTCAGC(-5/-2)
BglII        2262  AGATCT               A^GATCT
BpuAI        1372  GAAGAC               GAAGAC(2/6)
BsaI         1198  GGTCTC               GGTCTC(1/5)
BsaWI        1720  ACCGGA               [AT]^CCGG[AT]
BsiWI        1648  CGTACG               C^GTACG
BsrDI         259  GCAATG               GCAATG(2/0)
BsrFI        1062  GCCGGC               [GA]^CCGG[CT]
BstBI        1541  TTCGAA               TT^CGAA
BstEII       1248  GGTCACC              G^GT[ACGT]ACC
BstPI        1248  GGTCACC              G^GT[ACGT]ACC
Bsu36I        154  CCTGAGG              CC^T[ACGT]AGG
CfoI         1644  GCGC                 GCG^C
Cfr10I       1062  GCCGGC               [GA]^CCGG[CT]
Eco81I        154  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1248  GGTCACC              G^GT[ACGT]ACC
EcoRI        1265  GAATTC               G^AATTC
EcoRV        2123  GATATC               GAT^ATC
FauI         1561  CCCGC                CCCGC(4/6)
HaeII        1643  AGCGCC               [GA]GCGC^[CT]
HhaI         1644  GCGC                 GCG^C
HinP1I       1644  GCGC                 G^CGC
HincII       1121  GTCAAC               GT[CT]^[GA]AC
HindII       1121  GTCAAC               GT[CT]^[GA]AC
NaeI         1062  GCCGGC               GCC^GGC
NdeI         1958  CATATG               CA^TATG
NgoMIV       1062  GCCGGC               G^CCGGC
NspV         1541  TTCGAA               TT^CGAA
PaeR7I        266  CTCGAG               C^TCGAG
PmaCI         449  CACGTG               CAC^GTG
PmlI          449  CACGTG               CAC^GTG
SacI          269  GAGCTC               GAGCT^C
SapI         1399  GCTCTTC              GCTCTTC(1/4)
SexAI         491  ACCAGGT              A^CC[AT]GGT
SfuI         1541  TTCGAA               TT^CGAA
SnaBI         596  TACGTA               TAC^GTA
SpeI         1605  ACTAGT               A^CTAGT
XcmI         1498  CCACCATCCCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          266  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Asp700I       101  GAATCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1265  GAATTCTTTC          
AvaI          266  CTCGAG               C^[CT]CG[GA]G
             1742  CTCGGG              
BanII         269  GAGCTC               G[GA]GC[CT]^C
             1724  GAGCCC              
BciVI         171  GGATAC               GTATCC(6/5)
             1635  GGATAC              
BpmI         2300  CTCCAG               CTGGAG(16/14)
              701  CTGGAG              
BsaAI         449  CACGTG               [CT]AC^GT[GA]
              596  TACGTA              
BseRI         572  CTCCTC               GAGGAG(10/8)
             1913  CTCCTC              
BsgI         1256  GTGCAG               GTGCAG(16/14)
             1798  GTGCAG              
BsoBI         266  CTCGAG               C^[CT]CG[GA]G
             1742  CTCGGG              
BstAPI        436  GCAGAACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1564  GCAAATCATGC         
DsaI           38  CCATGG               C^C[GA][CT]GG
             1655  CCATGG              
HapII        1063  CCGG                 C^CGG
             1721  CCGG                
HpaII        1063  CCGG                 C^CGG
             1721  CCGG                
MspI         1063  CCGG                 C^CGG
             1721  CCGG                
NcoI           38  CCATGG               C^CATGG
             1655  CCATGG              
SphI          317  GCATGC               GCATG^C
             2271  GCATGC              
TaqI          267  TCGA                 T^CGA
             1542  TCGA                
TthHB8I       267  TCGA                 T^CGA
             1542  TCGA                
XmnI          101  GAATCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1265  GAATTCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BalI        
BanI        BbvI        BfaI        BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
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