Restriction map for pF1KB0026
 (Linear) (Six-base) MAPSORT of: KIBB0026.seq  Check: 9268  from: 1  to: 3174



KIBB0026.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     867    1888    3174 
   Size:        867    1021    1286

  Fragments arranged by size: 

                1286    1021     867
 
AatII G_ACGT'C

Cuts at:      0    2663    3174 
   Size:       2663     511
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922     946    1669    1819    2459    3174 
   Size:        922      24     723     150     640     715

  Fragments arranged by size: 

                 922     723     715     640     150      24
 
AfeI AGC'GCT

Cuts at:      0     767    3174 
   Size:        767    2407
 
AflII C'TTAA_G

Cuts at:      0     718    3174 
   Size:        718    2456
 
AleI CACnn'nnGTG

Cuts at:      0    1306    2329    2915    3174 
   Size:       1306    1023     586     259

  Fragments arranged by size: 

                1306    1023     586     259
 
AlwNI CAG_nnn'CTG

Cuts at:      0     180    1212    1609    2139    2196    2277    2370    2718 
   Size:        180    1032     397     530      57      81      93     348

Cuts at:   2718    3174 
   Size:        456

  Fragments arranged by size: 

                1032     530     456     397     348     180      93      81
                  57
 
ApaI G_GGCC'C

Cuts at:      0    1842    3174 
   Size:       1842    1332
 
ApaLI G'TGCA_C

Cuts at:      0     730    2281    3174 
   Size:        730    1551     893

  Fragments arranged by size: 

                1551     893     730
 
ApoI r'AATT_y

Cuts at:      0     268    1625    2251    3174 
   Size:        268    1357     626     923

  Fragments arranged by size: 

                1357     923     626     268
 
AvaI C'yCGr_G

Cuts at:      0    1267    1791    1905    3174 
   Size:       1267     524     114    1269

  Fragments arranged by size: 

                1269    1267     524     114
 
BamHI G'GATC_C

Cuts at:      0    1703    3174 
   Size:       1703    1471
 
BanI G'GyrC_C

Cuts at:      0    1472    1545    1900    2010    2779    3174 
   Size:       1472      73     355     110     769     395

  Fragments arranged by size: 

                1472     769     395     355     110      73
 
BanII G_rGCy'C

Cuts at:      0     775    1091    1427    1522    1574    1842    1912    2834 
   Size:        775     316     336      95      52     268      70     922

Cuts at:   2834    3021    3174 
   Size:        187     153

  Fragments arranged by size: 

                 922     775     336     316     268     187     153      95
                  70      52
 
BbeI G_GCGC'C

Cuts at:      0    1476    2014    3174 
   Size:       1476     538    1160

  Fragments arranged by size: 

                1476    1160     538
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1453    2754    3174 
   Size:       1453    1301     420

  Fragments arranged by size: 

                1453    1301     420
 
BbvCI CC'TCA_GC

Cuts at:      0    1155    2158    2470    3174 
   Size:       1155    1003     312     704

  Fragments arranged by size: 

                1155    1003     704     312
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1330    1364    1886    1920    2559    2593    3174 
   Size:       1330      34     522      34     639      34     581

  Fragments arranged by size: 

                1330     639     581     522      34      34      34
 
BclI T'GATC_A

Cuts at:      0     134     182    1463    3174 
   Size:        134      48    1281    1711

  Fragments arranged by size: 

                1711    1281     134      48
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1891    2777    3174 
   Size:       1891     886     397

  Fragments arranged by size: 

                1891     886     397
 
BglII A'GATC_T

Cuts at:      0    1163    3174 
   Size:       1163    2011
 
Bme1580I G_kGCm'C

Cuts at:      0     734     988    1842    2034    2285    2782    3157    3174 
   Size:        734     254     854     192     251     497     375      17

  Fragments arranged by size: 

                 854     734     497     375     254     251     192      17
            
 
BmgBI CAC'GTC

Cuts at:      0    1644    3174 
   Size:       1644    1530
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1393    3174 
   Size:       1393    1781
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2817    3174 
   Size:       2817     357
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1105    1555    1846    2026    2056    2713    2846    2898 
   Size:       1105     450     291     180      30     657     133      52

Cuts at:   2898    3043    3174 
   Size:        145     131

  Fragments arranged by size: 

                1105     657     450     291     180     145     133     131
                  52      30
 
Bpu10I CC'TnA_GC

Cuts at:      0    1155    1516    2158    2470    3174 
   Size:       1155     361     642     312     704

  Fragments arranged by size: 

                1155     704     642     361     312
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     372    1054    3174 
   Size:         44     328     682    2120

  Fragments arranged by size: 

                2120     682     328      44
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     792    2093    3174 
   Size:        792    1301    1081

  Fragments arranged by size: 

                1301    1081     792
 
BsaHI Gr'CG_yC

Cuts at:      0     615    1219    1473    2011    2660    2935    3174 
   Size:        615     604     254     538     649     275     239

  Fragments arranged by size: 

                 649     615     604     538     275     254     239
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       8      38    1045    1075    1798    1828    2146    2176 
   Size:          8      30    1007      30     723      30     318      30

Cuts at:   2176    2842    2872    3174 
   Size:        666      30     302

  Fragments arranged by size: 

                1007     723     666     318     302      30      30      30
                  30      30       8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     304     735    1435    1447    1575    2140    2365 
   Size:         79     225     431     700      12     128     565     225

Cuts at:   2365    3050    3076    3174 
   Size:        685      26      98

  Fragments arranged by size: 

                 700     685     565     431     225     225     128      98
                  79      26      12
 
BseYI C'CCAG_C

Cuts at:      0     401     470     768    1686    2775    2783    3174 
   Size:        401      69     298     918    1089       8     391

  Fragments arranged by size: 

                1089     918     401     391     298      69       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     892    2563    3174 
   Size:        892    1671     611

  Fragments arranged by size: 

                1671     892     611
 
BsiEI CG_ry'CG

Cuts at:      0    2429    3174 
   Size:       2429     745
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     734    1522    2285    2684    2834    3021    3174 
   Size:        164     570     788     763     399     150     187     153

  Fragments arranged by size: 

                 788     763     570     399     187     164     153     150
            
 
BsmI GAATG_Cn'

Cuts at:      0     235    3174 
   Size:        235    2939
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1639    3174 
   Size:       1639    1535
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     734     775     988    1091    1427    1522    1574 
   Size:        164     570      41     213     103     336      95      52

Cuts at:   1574    1842    1912    2034    2285    2684    2782    2834    3021 
   Size:        268      70     122     251     399      98      52     187

Cuts at:   3021    3157    3174 
   Size:        136      17

  Fragments arranged by size: 

                 570     399     336     268     251     213     187     164
                 136     122     103      98      95      70      52      52
                  41      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     860     867    1888    2147    2276    3155    3174 
   Size:        860       7    1021     259     129     879      19

  Fragments arranged by size: 

                1021     879     860     259     129      19       7
 
BsrBI CCG'CTC

Cuts at:      0       8    3174 
   Size:          8    3166
 
BsrDI GCAATG_nn'

Cuts at:      0     329    3174 
   Size:        329    2845
 
BsrGI T'GTAC_A

Cuts at:      0    2432    3174 
   Size:       2432     742
 
BssSI C'ACGA_G

Cuts at:      0     650    2568    3174 
   Size:        650    1918     606

  Fragments arranged by size: 

                1918     650     606
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1212    2054    3174 
   Size:       1212     842    1120

  Fragments arranged by size: 

                1212    1120     842
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     207     768     972    1503    1686    1981    2917    2922 
   Size:        207     561     204     531     183     295     936       5

Cuts at:   2922    3174 
   Size:        252

  Fragments arranged by size: 

                 936     561     531     295     252     207     204     183
                   5
 
BstYI r'GATC_y

Cuts at:      0     111    1163    1703    1760    1985    2634    3174 
   Size:        111    1052     540      57     225     649     540

  Fragments arranged by size: 

                1052     649     540     540     225     111      57
 
Bsu36I CC'TnA_GG

Cuts at:      0     992    3174 
   Size:        992    2182
 
BtgI C'CryG_G

Cuts at:      0     488    1307    3174 
   Size:        488     819    1867

  Fragments arranged by size: 

                1867     819     488
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     216    1064    2430    3174 
   Size:        216     848    1366     744

  Fragments arranged by size: 

                1366     848     744     216
 
BtsI GCAGTG_nn'

Cuts at:      0      18     430     891    2371    3174 
   Size:         18     412     461    1480     803

  Fragments arranged by size: 

                1480     803     461     412      18
 
ClaI AT'CG_AT

Cuts at:      0      80    3174 
   Size:         80    3094
 
DraIII CAC_nnn'GTG

Cuts at:      0    3167    3174 
   Size:       3167       7
 
EaeI y'GGCC_r

Cuts at:      0     866    1170    1358    1766    1892    2426    3174 
   Size:        866     304     188     408     126     534     748

  Fragments arranged by size: 

                 866     748     534     408     304     188     126
 
EagI C'GGCC_G

Cuts at:      0    2426    3174 
   Size:       2426     748
 
EarI CTCTTCn'nnn_

Cuts at:      0     683    1535    3068    3174 
   Size:        683     852    1533     106

  Fragments arranged by size: 

                1533     852     683     106
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1819    1930    3174 
   Size:       1819     111    1244

  Fragments arranged by size: 

                1819    1244     111
 
EcoICRI GAG'CTC

Cuts at:      0    1520    2832    3019    3174 
   Size:       1520    1312     187     155

  Fragments arranged by size: 

                1520    1312     187     155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922     946    1105    1555    1669    1819    1846    2026 
   Size:        922      24     159     450     114     150      27     180

Cuts at:   2026    2056    2459    2713    2846    2898    3043    3174 
   Size:         30     403     254     133      52     145     131

  Fragments arranged by size: 

                 922     450     403     254     180     159     150     145
                 133     131     114      52      30      27      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      99    1099    1267    3174 
   Size:         99    1000     168    1907

  Fragments arranged by size: 

                1907    1000     168      99
 
EcoO109I rG'GnC_Cy

Cuts at:      0     564    1142    1809    2193    2715    2946    3174 
   Size:        564     578     667     384     522     231     228

  Fragments arranged by size: 

                 667     578     564     522     384     231     228
 
EcoRV GAT'ATC

Cuts at:      0     630    3174 
   Size:        630    2544
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     720    2778    3174 
   Size:        720    2058     396

  Fragments arranged by size: 

                2058     720     396
 
HaeII r_GCGC'y

Cuts at:      0     133     769    1476    1610    1694    2014    2118    2902 
   Size:        133     636     707     134      84     320     104     784

Cuts at:   2902    3174 
   Size:        272

  Fragments arranged by size: 

                 784     707     636     320     272     134     133     104
                  84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40     466     498    1045    1077    1798    1830 
   Size:          8      32     426      32     547      32     721      32

Cuts at:   1830    2090    2122    2146    2178    2863    2895    2983    3015 
   Size:        260      32      24      32     685      32      88      32

Cuts at:   3015    3174 
   Size:        159

  Fragments arranged by size: 

                 721     685     547     426     260     159      88      32
                  32      32      32      32      32      32      32      24
                   8
 
KasI G'GCGC_C

Cuts at:      0    1472    2010    3174 
   Size:       1472     538    1164

  Fragments arranged by size: 

                1472    1164     538
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      13    3174 
   Size:         13    3161
 
MscI TGG'CCA

Cuts at:      0     868    1360    1768    3174 
   Size:        868     492     408    1406

  Fragments arranged by size: 

                1406     868     492     408
 
MslI CAynn'nnrTG

Cuts at:      0     808    1252    1306    1366    1501    2329    2677    2749 
   Size:        808     444      54      60     135     828     348      72

Cuts at:   2749    2915    2960    3174 
   Size:        166      45     214

  Fragments arranged by size: 

                 828     808     444     348     214     166     135      72
                  60      54      45
 
MspA1I CmG'CkG

Cuts at:      0     714    1195    1375    1736    2232    2817    3174 
   Size:        714     481     180     361     496     585     357

  Fragments arranged by size: 

                 714     585     496     481     361     357     180
 
NarI GG'CG_CC

Cuts at:      0    1473    2011    3174 
   Size:       1473     538    1163

  Fragments arranged by size: 

                1473    1163     538
 
NcoI C'CATG_G

Cuts at:      0     488    3174 
   Size:        488    2686
 
NspI r_CATG'y

Cuts at:      0     299     348     847    1017    2088    2146    2217    2965 
   Size:        299      49     499     170    1071      58      71     748

Cuts at:   2965    3174 
   Size:        209

  Fragments arranged by size: 

                1071     748     499     299     209     170      71      58
                  49
 
PasI CC'CwG_GG

Cuts at:      0    1485    3174 
   Size:       1485    1689
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2421    3174 
   Size:       2421     753
 
PfoI T'CCnGG_A

Cuts at:      0     173     218    1823    2630    3174 
   Size:        173      45    1605     807     544

  Fragments arranged by size: 

                1605     807     544     173      45
 
PpuMI rG'GwC_Cy

Cuts at:      0    1142    1809    2946    3174 
   Size:       1142     667    1137     228

  Fragments arranged by size: 

                1142    1137     667     228
 
PshAI GACnn'nnGTC

Cuts at:      0    2563    2951    3174 
   Size:       2563     388     223

  Fragments arranged by size: 

                2563     388     223
 
PspOMI G'GGCC_C

Cuts at:      0    1838    3174 
   Size:       1838    1336
 
PstI C_TGCA'G

Cuts at:      0     425    1208    1735    1961    2273    3174 
   Size:        425     783     527     226     312     901

  Fragments arranged by size: 

                 901     783     527     425     312     226
 
PvuII CAG'CTG

Cuts at:      0     714    1195    1375    2232    3174 
   Size:        714     481     180     857     942

  Fragments arranged by size: 

                 942     857     714     481     180
 
SacI G_AGCT'C

Cuts at:      0    1522    2834    3021    3174 
   Size:       1522    1312     187     153

  Fragments arranged by size: 

                1522    1312     187     153
 
SbfI CC_TGCA'GG

Cuts at:      0    1961    3174 
   Size:       1961    1213
 
ScaI AGT'ACT

Cuts at:      0    2053    3174 
   Size:       2053    1121
 
SexAI A'CCwGG_T

Cuts at:      0    1389    3174 
   Size:       1389    1785
 
SfcI C'TryA_G

Cuts at:      0     421     457    1204    1731    1957    2269    3174 
   Size:        421      36     747     527     226     312     905

  Fragments arranged by size: 

                 905     747     527     421     312     226      36
 
SfoI GGC'GCC

Cuts at:      0    1474    2012    3174 
   Size:       1474     538    1162

  Fragments arranged by size: 

                1474    1162     538
 
SmlI C'TyrA_G

Cuts at:      0      59     351     718    1069    3174 
   Size:         59     292     367     351    2105

  Fragments arranged by size: 

                2105     367     351     292      59
 
SphI G_CATG'C

Cuts at:      0     299     847    3174 
   Size:        299     548    2327

  Fragments arranged by size: 

                2327     548     299
 
StuI AGG'CCT

Cuts at:      0     466     829    1153    3174 
   Size:        466     363     324    2021

  Fragments arranged by size: 

                2021     466     363     324
 
StyI C'CwwG_G

Cuts at:      0     488     704     941    1427    1994    2546    3174 
   Size:        488     216     237     486     567     552     628

  Fragments arranged by size: 

                 628     567     552     488     486     237     216
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     556    2797    2972    3174 
   Size:        556    2241     175     202

  Fragments arranged by size: 

                2241     556     202     175
 
TatI w'GTAC_w

Cuts at:      0     671    1877    2051    2432    3174 
   Size:        671    1206     174     381     742

  Fragments arranged by size: 

                1206     742     671     381     174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     922     954    3174 
   Size:        922      32    2220

  Fragments arranged by size: 

                2220     922      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      22    2596    3174 
   Size:         22    2574     578

  Fragments arranged by size: 

                2574     578      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     555     972    1503    1530    2001    3174 
   Size:        555     417     531      27     471    1173

  Fragments arranged by size: 

                1173     555     531     471     417      27
 
XmnI GAAnn'nnTTC

Cuts at:      0    1627    3174 
   Size:       1627    1547
 
ZraI GAC'GTC

Cuts at:      0    2661    3174 
   Size:       2661     513
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AflII     AleI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NspI     PasI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SacI     SbfI     ScaI    SexAI     SfcI     SfoI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI   AflIII     AgeI     AhdI     AlfI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI    BciVI    BfrBI     BlpI
     BmtI    BsaAI    BsaBI    BsaWI    BsiWI    BspEI    BspHI    BsrFI
   BssHII    BstBI   BstEII  BstZ17I    CspCI     DraI     DrdI    EcoRI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI
     XhoI     XmaI
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