Restriction map for pF1KB9773
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BfaI        BfrI        BlnI        
BpuAI       BsaI        BsaWI       BseAI       BsiWI       
Bsp1407I    BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
Bsu36I      CpoI        DraI        Eam1105I    Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MaeI        MfeI        MluI        MroI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuII       RcaI        
RsrII       SacI        SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Asp700I       714  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         788  TGCGCA               TGC^GCA
BalI            2  TGGCCA               TGG^CCA
BcgI         1434  CGACATCGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         1108  GCCTACGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1380  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1380  GCTGAGC              GC^T[ACGT]AGC
BsaAI         121  TACGTG               [CT]AC^GT[GA]
BsmBI        1210  GAGACG               CGTCTC(1/5)
BsmI          337  GCATTC               GAATGC(1/-1)
BspDI        1438  ATCGAT               AT^CGAT
BsrI          266  ACTGG                ACTGG(1/-1)
BssSI        1734  CTCGTG               CACGAG(-5/-1)
CelII        1380  GCTGAGC              GC^T[ACGT]AGC
ClaI         1438  ATCGAT               AT^CGAT
DraIII        718  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          816  GACCCTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1647  GAAGAG               CTCTTC(1/4)
FspI          788  TGCGCA               TGC^GCA
Ksp632I      1647  GAAGAG               CTCTTC(1/4)
KspI           11  CCGCGG               CCGC^GG
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
NaeI          438  GCCGGC               GCC^GGC
NcoI          362  CCATGG               C^CATGG
NgoMIV        438  GCCGGC               G^CCGGC
PflMI         717  CCACTTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1714  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1048  CTGCAG               CTGCA^G
SacII          11  CCGCGG               CCGC^GG
SapI         1647  GAAGAGC              GCTCTTC(1/4)
SfcI         1048  CTGCAG               C^T[GA][CT]AG
SmlI         1672  CTCAAG               C^T[CT][GA]AG
TfiI          990  GATTC                G^A[AT]TC
TspRI         265  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        717  CCACTTCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          714  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI          967  GACGCC               G[GA]^CG[CT]C
             1744  GACGCC              
AflIII        617  ACGTGT               A^C[GA][CT]GT
              881  ACACGT              
AlwI         1317  GATCC                GGATC(4/5)
              512  GGATC               
AlwNI        1039  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1165  CAGGACCTG           
AspI          816  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              997  GACCTGGTC           
BglII         353  AGATCT               A^GATCT
             1277  AGATCT              
BsaBI          18  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1500  GATCACCATC          
BsaHI         967  GACGCC               G[GA]^CG[CT]C
             1744  GACGCC              
BsmAI         293  GAGAC                GTCTC(1/5)
             1210  GAGAC               
BsrBI         142  GAGCGG               CCGCTC(-3/-3)
             1383  GAGCGG              
BstEII        414  GGTGACC              G^GT[ACGT]ACC
             1425  GGTGACC             
BstPI         414  GGTGACC              G^GT[ACGT]ACC
             1425  GGTGACC             
BstYI         353  AGATCT               [GA]^GATC[CT]
             1277  AGATCT              
EcoO65I       414  GGTGACC              G^GT[ACGT]ACC
             1425  GGTGACC             
Hin1I         967  GACGCC               G[GA]^CG[CT]C
             1744  GACGCC              
MamI           18  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1500  GATCACCATC          
MflI          353  AGATCT               [GA]^GATC[CT]
             1277  AGATCT              
MslI          679  CACCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1134  CATCCTGATG          
MspA1I         11  CCGCGG               C[AC]G^C[GT]G
             1821  CAGCGG              
PflFI         816  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              997  GACCTGGTC           
PvuI           17  CGATCG               CGAT^CG
              632  CGATCG              
SexAI         170  ACCAGGT              A^CC[AT]GGT
              998  ACCTGGT             
SmaI         1306  CCCGGG               CCC^GGG
             1849  CCCGGG              
Tth111I       816  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              997  GACCTGGTC           
XhoII         353  AGATCT               [GA]^GATC[CT]
             1277  AGATCT              
XmaCI        1306  CCCGGG               C^CCGGG
             1849  CCCGGG              
XmaI         1306  CCCGGG               C^CCGGG
             1849  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BclI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TseI        
Tsp45I      TthHB8I     XcmI        
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