Restriction map for pF1KB9773
 (Linear) (Six-base) MAPSORT of: KIBB9773.seq  Check: 2271  from: 1  to: 1923



KIBB9773.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     300    1791    1923 
   Size:        300    1491     132

  Fragments arranged by size: 

                1491     300     132
 
AflIII A'CryG_T

Cuts at:      0     617     881    1923 
   Size:        617     264    1042

  Fragments arranged by size: 

                1042     617     264
 
AleI CACnn'nnGTG

Cuts at:      0     683    1923 
   Size:        683    1240
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1044    1170    1923 
   Size:       1044     126     753

  Fragments arranged by size: 

                1044     753     126
 
AvaI C'yCGr_G

Cuts at:      0     346    1306    1849    1923 
   Size:        346     960     543      74

  Fragments arranged by size: 

                 960     543     346      74
 
BanI G'GyrC_C

Cuts at:      0      34     607    1837    1923 
   Size:         34     573    1230      86

  Fragments arranged by size: 

                1230     573      86      34
 
BanII G_rGCy'C

Cuts at:      0     500     527     947    1018    1673    1872    1923 
   Size:        500      27     420      71     655     199      51

  Fragments arranged by size: 

                 655     500     420     199      71      51      27
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1423    1457    1923 
   Size:       1423      34     466

  Fragments arranged by size: 

                1423     466      34
 
BclI T'GATC_A

Cuts at:      0     281     428    1238    1923 
   Size:        281     147     810     685

  Fragments arranged by size: 

                 810     685     281     147
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1114    1923 
   Size:       1114     809
 
BglII A'GATC_T

Cuts at:      0     353    1277    1923 
   Size:        353     924     646

  Fragments arranged by size: 

                 924     646     353
 
BlpI GC'TnA_GC

Cuts at:      0    1381    1923 
   Size:       1381     542
 
Bme1580I G_kGCm'C

Cuts at:      0      37     610    1415    1923 
   Size:         37     573     805     508

  Fragments arranged by size: 

                 805     573     508      37
 
BmgBI CAC'GTC

Cuts at:      0     618     884    1185    1923 
   Size:        618     266     301     738

  Fragments arranged by size: 

                 738     618     301     266
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     372     870     933    1749    1923 
   Size:        372     498      63     816     174

  Fragments arranged by size: 

                 816     498     372     174      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865     961    1228    1645    1816    1923 
   Size:        865      96     267     417     171     107

  Fragments arranged by size: 

                 865     417     267     171     107      96
 
Bpu10I CC'TnA_GC

Cuts at:      0    1528    1871    1923 
   Size:       1528     343      52

  Fragments arranged by size: 

                1528     343      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1657    1923 
   Size:       1657     266
 
BsaAI yAC'GTr

Cuts at:      0     123    1923 
   Size:        123    1800
 
BsaBI GATnn'nnATC

Cuts at:      0      22    1504    1923 
   Size:         22    1482     419

  Fragments arranged by size: 

                1482     419      22
 
BsaHI Gr'CG_yC

Cuts at:      0     968    1745    1923 
   Size:        968     777     178

  Fragments arranged by size: 

                 968     777     178
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     343     373    1789    1819    1923 
   Size:        343      30    1416      30     104

  Fragments arranged by size: 

                1416     343     104      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     364     742     922    1552    1923 
   Size:        364     378     180     630     371

  Fragments arranged by size: 

                 630     378     371     364     180
 
BseYI C'CCAG_C

Cuts at:      0     115    1200    1923 
   Size:        115    1085     723

  Fragments arranged by size: 

                1085     723     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     328    1057    1144    1183    1576    1612    1923 
   Size:        268      60     729      87      39     393      36     311

  Fragments arranged by size: 

                 729     393     311     268      87      60      39      36
            
 
BsiEI CG_ry'CG

Cuts at:      0      20     225     533     635     668    1217    1923 
   Size:         20     205     308     102      33     549     706

  Fragments arranged by size: 

                 706     549     308     205     102      33      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     587     919    1483    1781    1923 
   Size:        587     332     564     298     142

  Fragments arranged by size: 

                 587     564     332     298     142
 
BsmI GAATG_Cn'

Cuts at:      0     337    1923 
   Size:        337    1586
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1204    1923 
   Size:       1204     719
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     500     527     587     610     919     947    1018 
   Size:         37     463      27      60      23     309      28      71

Cuts at:   1018    1415    1483    1673    1781    1872    1923 
   Size:        397      68     190     108      91      51

  Fragments arranged by size: 

                 463     397     309     190     108      91      71      68
                  60      51      37      28      27      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     300    1178    1791    1923 
   Size:        300     878     613     132

  Fragments arranged by size: 

                 878     613     300     132
 
BsrBI CCG'CTC

Cuts at:      0     144    1385    1923 
   Size:        144    1241     538

  Fragments arranged by size: 

                1241     538     144
 
BsrFI r'CCGG_y

Cuts at:      0     438    1483    1840    1923 
   Size:        438    1045     357      83

  Fragments arranged by size: 

                1045     438     357      83
 
BssSI C'ACGA_G

Cuts at:      0    1734    1923 
   Size:       1734     189
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     797    1044    1170    1923 
   Size:        797     247     126     753

  Fragments arranged by size: 

                 797     753     247     126
 
BstEII G'GTnAC_C

Cuts at:      0     414    1425    1923 
   Size:        414    1011     498

  Fragments arranged by size: 

                1011     498     414
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     123     685    1140    1755    1923 
   Size:        123     562     455     615     168

  Fragments arranged by size: 

                 615     562     455     168     123
 
BstYI r'GATC_y

Cuts at:      0     353    1277    1923 
   Size:        353     924     646

  Fragments arranged by size: 

                 924     646     353
 
BtgI C'CryG_G

Cuts at:      0      11     362     527     662     947    1469    1655    1923 
   Size:         11     351     165     135     285     522     186     268

  Fragments arranged by size: 

                 522     351     285     268     186     165     135      11
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      69     525    1923 
   Size:         69     456    1398

  Fragments arranged by size: 

                1398     456      69
 
ClaI AT'CG_AT

Cuts at:      0    1439    1923 
   Size:       1439     484
 
DraIII CAC_nnn'GTG

Cuts at:      0     723    1923 
   Size:        723    1200
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     822    1923 
   Size:        822    1101
 
EaeI y'GGCC_r

Cuts at:      0       2     161     222     530     665    1130    1214    1466 
   Size:          2     159      61     308     135     465      84     252

Cuts at:   1466    1757    1923 
   Size:        291     166

  Fragments arranged by size: 

                 465     308     291     252     166     159     135      84
                  61       2
 
EagI C'GGCC_G

Cuts at:      0     222     530     665    1214    1923 
   Size:        222     308     135     549     709

  Fragments arranged by size: 

                 709     549     308     222     135
 
EarI CTCTTCn'nnn_

Cuts at:      0    1642    1923 
   Size:       1642     281
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1579    1923 
   Size:       1579     344
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865     961    1228    1645    1816    1923 
   Size:        865      96     267     417     171     107

  Fragments arranged by size: 

                 865     417     267     171     107      96
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     801     862    1531    1923 
   Size:        801      61     669     392

  Fragments arranged by size: 

                 801     669     392      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     815    1167    1876    1900    1923 
   Size:        815     352     709      24      23

  Fragments arranged by size: 

                 815     709     352      24      23
 
FspI TGC'GCA

Cuts at:      0     790    1923 
   Size:        790    1133
 
HaeII r_GCGC'y

Cuts at:      0     181     465    1374    1655    1923 
   Size:        181     284     909     281     268

  Fragments arranged by size: 

                 909     284     281     268     181
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375    1591    1623    1923 
   Size:        343      32    1216      32     300

  Fragments arranged by size: 

                1216     343     300      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     233     944    1319    1923 
   Size:         87     146     711     375     604

  Fragments arranged by size: 

                 711     604     375     146      87
 
MscI TGG'CCA

Cuts at:      0       4    1923 
   Size:          4    1919
 
MslI CAynn'nnrTG

Cuts at:      0     683    1138    1923 
   Size:        683     455     785

  Fragments arranged by size: 

                 785     683     455
 
MspA1I CmG'CkG

Cuts at:      0      13    1823    1923 
   Size:         13    1810     100

  Fragments arranged by size: 

                1810     100      13
 
NaeI GCC'GGC

Cuts at:      0     440    1923 
   Size:        440    1483
 
NcoI C'CATG_G

Cuts at:      0     362    1923 
   Size:        362    1561
 
NgoMIV G'CCGG_C

Cuts at:      0     438    1923 
   Size:        438    1485
 
PasI CC'CwG_GG

Cuts at:      0    1403    1923 
   Size:       1403     520
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     723    1923 
   Size:        723    1200
 
PpuMI rG'GwC_Cy

Cuts at:      0     815    1167    1876    1923 
   Size:        815     352     709      47

  Fragments arranged by size: 

                 815     709     352      47
 
PshAI GACnn'nnGTC

Cuts at:      0    1718    1923 
   Size:       1718     205
 
PstI C_TGCA'G

Cuts at:      0    1052    1923 
   Size:       1052     871
 
PvuI CG_AT'CG

Cuts at:      0      20     635    1923 
   Size:         20     615    1288

  Fragments arranged by size: 

                1288     615      20
 
SacII CC_GC'GG

Cuts at:      0      14    1923 
   Size:         14    1909
 
SanDI GG'GwC_CC

Cuts at:      0    1876    1923 
   Size:       1876      47
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1642    1923 
   Size:       1642     281
 
SexAI A'CCwGG_T

Cuts at:      0     170     998    1923 
   Size:        170     828     925

  Fragments arranged by size: 

                 925     828     170
 
SfcI C'TryA_G

Cuts at:      0    1048    1923 
   Size:       1048     875
 
SmaI CCC'GGG

Cuts at:      0    1308    1851    1923 
   Size:       1308     543      72

  Fragments arranged by size: 

                1308     543      72
 
SmlI C'TyrA_G

Cuts at:      0    1672    1923 
   Size:       1672     251
 
StyI C'CwwG_G

Cuts at:      0     302     332     362     473    1031    1754    1880    1923 
   Size:        302      30      30     111     558     723     126      43

  Fragments arranged by size: 

                 723     558     302     126     111      43      30      30
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     673     691     847    1923 
   Size:        673      18     156    1076

  Fragments arranged by size: 

                1076     673     156      18
 
TatI w'GTAC_w

Cuts at:      0    1571    1923 
   Size:       1571     352
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1789    1821    1923 
   Size:       1789      32     102

  Fragments arranged by size: 

                1789     102      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     819    1000    1923 
   Size:        819     181     923

  Fragments arranged by size: 

                 923     819     181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     171    1620    1840    1923 
   Size:        171    1449     220      83

  Fragments arranged by size: 

                1449     220     171      83
 
XmaI C'CCGG_G

Cuts at:      0    1306    1849    1923 
   Size:       1306     543      74

  Fragments arranged by size: 

                1306     543      74
 
XmnI GAAnn'nnTTC

Cuts at:      0     718    1923 
   Size:        718    1205
 

 Enzymes that do cut:

     AarI   AflIII     AleI    AlwNI     AvaI     BanI    BanII     BcgI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     ClaI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI    PpuMI    PshAI
     PstI     PvuI    SacII    SanDI     SapI    SexAI     SfcI     SmaI
     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
    BfrBI     BmrI     BmtI     BsaI    BsaWI    BsiWI    BspEI    BspHI
    BsrDI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I     BtsI    CspCI
     DraI  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI     SalI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XbaI     XhoI     ZraI
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