Restriction map for pF1KB6855
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1407I    BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstF5I      
BstUI       BstXI       BstZ17I     Cfr10I      CpoI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoO109I    EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AocI          354  CCTCAGG              CC^T[ACGT]AGG
Asp700I       153  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          30  GGTCC                G^G[AT]CC
BanI           67  GGCACC               G^G[CT][GA]CC
BbvCI         360  GCTGAGG              CCTCAGC(-5/-2)
BbvI           86  GCTGC                GCAGC(8/12)
BglII         126  AGATCT               A^GATCT
BlpI            8  GCTCAGC              GC^T[ACGT]AGC
BmyI           66  GGGCAC               G[AGT]GC[ACT]^C
Bpu1102I        8  GCTCAGC              GC^T[ACGT]AGC
BseRI         274  GAGGAG               GAGGAG(10/8)
BsgI           87  CTGCAC               GTGCAG(16/14)
BsmI          429  GAATGC               GAATGC(1/-1)
Bsp1286I       66  GGGCAC               G[AGT]GC[ACT]^C
BspDI         380  ATCGAT               AT^CGAT
BstEII        226  GGTTACC              G^GT[ACGT]ACC
BstPI         226  GGTTACC              G^GT[ACGT]ACC
BstYI         126  AGATCT               [GA]^GATC[CT]
Bsu36I        354  CCTCAGG              CC^T[ACGT]AGG
Cac8I          52  GCCAGC               GC[ACGT]^[ACGT]GC
CelII           8  GCTCAGC              GC^T[ACGT]AGC
ClaI          380  ATCGAT               AT^CGAT
DsaI          413  CCGTGG               C^C[GA][CT]GG
Eco81I        354  CCTCAGG              CC^T[ACGT]AGG
EcoNI         270  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       226  GGTTACC              G^GT[ACGT]ACC
Fnu4HI         86  GCTGC                GC^[ACGT]GC
HaeII         371  GGCGCT               [GA]GCGC^[CT]
HinfI         202  GAGTC                G^A[ACGT]TC
ItaI           86  GCTGC                GC^[ACGT]GC
MaeIII        227  GTTAC                ^GT[ACGT]AC
MflI          126  AGATCT               [GA]^GATC[CT]
MslI          513  CATGACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          111  GCCTAACATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV          67  GGCACC               GG[ACGT]^[ACGT]CC
PleI          202  GAGTC                GAGTC(4/5)
TseI           86  GCTGC                G^C[AT]GC
TspRI          14  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         126  AGATCT               [GA]^GATC[CT]
XmnI          153  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwI          258  GATCC                GGATC(4/5)
              174  GGATC               
BbsI          153  GAAGAC               GAAGAC(2/6)
              348  GAAGAC              
BpmI          211  CTGGAG               CTGGAG(16/14)
              271  CTGGAG              
BpuAI         153  GAAGAC               GAAGAC(2/6)
              348  GAAGAC              
BspMI         107  ACCTGC               ACCTGC(4/8)
              420  GCAGGT              
CfoI          319  GCGC                 GCG^C
              372  GCGC                
Cfr13I         30  GGTCC                G^G[ACGT]CC
               50  GGGCC               
HhaI          319  GCGC                 GCG^C
              372  GCGC                
HinP1I        319  GCGC                 G^CGC
              372  GCGC                
MspA1I         84  CAGCTG               C[AC]G^C[GT]G
              178  CAGCTG              
NspI          116  ACATGC               [GA]CATG^[CT]
              321  GCATGT              
PvuII          84  CAGCTG               CAG^CTG
              178  CAGCTG              
Sau96I         30  GGTCC                G^G[ACGT]CC
               50  GGGCC               
TaqI          123  TCGA                 T^CGA
              381  TCGA                
TthHB8I       123  TCGA                 T^CGA
              381  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstNI       DdeI        DpnII       EcoRII      
HaeIII      HphI        MboI        MboII       MnlI        
MseI        MvaI        NdeII       NlaIII      Sau3AI      
ScrFI       SfaNI       Tru9I       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com