Restriction map for pF1KB7717
 (Linear) (Six-base) MAPSORT of: KIBB7717.seq  Check: 8218  from: 1  to: 1275



KIBB7717.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     407     612    1275 
   Size:        290     117     205     663

  Fragments arranged by size: 

                 663     290     205     117
 
AhdI GACnn_n'nnGTC

Cuts at:      0     723    1275 
   Size:        723     552
 
AlwNI CAG_nnn'CTG

Cuts at:      0     530    1275 
   Size:        530     745
 
ApoI r'AATT_y

Cuts at:      0     370     494     746     830    1275 
   Size:        370     124     252      84     445

  Fragments arranged by size: 

                 445     370     252     124      84
 
AvaI C'yCGr_G

Cuts at:      0     452    1275 
   Size:        452     823
 
BanI G'GyrC_C

Cuts at:      0    1142    1275 
   Size:       1142     133
 
BanII G_rGCy'C

Cuts at:      0     682    1275 
   Size:        682     593
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      56     544    1275 
   Size:         56     488     731

  Fragments arranged by size: 

                 731     488      56
 
BbvCI CC'TCA_GC

Cuts at:      0     646    1275 
   Size:        646     629
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      37      71    1275 
   Size:         37      34    1204

  Fragments arranged by size: 

                1204      37      34
 
BglII A'GATC_T

Cuts at:      0     225    1275 
   Size:        225    1050
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     285     596     680    1275 
   Size:        285     311      84     595

  Fragments arranged by size: 

                 595     311     285      84
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     282     284    1275 
   Size:        282       2     991

  Fragments arranged by size: 

                 991     282       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     776     860    1112    1275 
   Size:        151     625      84     252     163

  Fragments arranged by size: 

                 625     252     163     151      84
 
Bpu10I CC'TnA_GC

Cuts at:      0     646    1275 
   Size:        646     629
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256    1275 
   Size:        256    1019
 
BseYI C'CCAG_C

Cuts at:      0    1006    1275 
   Size:       1006     269
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     711    1275 
   Size:        711     564
 
BsiHKAI G_wGCw'C

Cuts at:      0     703    1275 
   Size:        703     572
 
BsmI GAATG_Cn'

Cuts at:      0     343     526    1114    1123    1198    1275 
   Size:        343     183     588       9      75      77

  Fragments arranged by size: 

                 588     343     183      77      75       9
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     983    1275 
   Size:        983     292
 
Bsp1286I G_dGCh'C

Cuts at:      0     682     703    1275 
   Size:        682      21     572

  Fragments arranged by size: 

                 682     572      21
 
BsrBI CCG'CTC

Cuts at:      0    1058    1275 
   Size:       1058     217
 
BsrFI r'CCGG_y

Cuts at:      0    1145    1275 
   Size:       1145     130
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     698    1118    1275 
   Size:        698     420     157

  Fragments arranged by size: 

                 698     420     157
 
BstBI TT'CG_AA

Cuts at:      0      47    1275 
   Size:         47    1228
 
BstYI r'GATC_y

Cuts at:      0     225    1082    1275 
   Size:        225     857     193

  Fragments arranged by size: 

                 857     225     193
 
BtgI C'CryG_G

Cuts at:      0      59     214    1275 
   Size:         59     155    1061

  Fragments arranged by size: 

                1061     155      59
 
BtsI GCAGTG_nn'

Cuts at:      0     531     699    1275 
   Size:        531     168     576

  Fragments arranged by size: 

                 576     531     168
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     765     800    1275 
   Size:        765      35     475

  Fragments arranged by size: 

                 765     475      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1202    1275 
   Size:       1202      73
 
EaeI y'GGCC_r

Cuts at:      0     253    1275 
   Size:        253    1022
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     193    1275 
   Size:         71     122    1082

  Fragments arranged by size: 

                1082     122      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     290     407     612     776     860    1112    1275 
   Size:        151     139     117     205     164      84     252     163

  Fragments arranged by size: 

                 252     205     164     163     151     139     117      84
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115    1275 
   Size:        115    1160
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108    1011    1275 
   Size:        108     903     264

  Fragments arranged by size: 

                 903     264     108
 
EcoRI G'AATT_C

Cuts at:      0     746     830    1275 
   Size:        746      84     445

  Fragments arranged by size: 

                 746     445      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     210     242     301     333     541     573     709     741 
   Size:        210      32      59      32     208      32     136      32

Cuts at:    741     793     825    1045    1077    1275 
   Size:         52      32     220      32     198

  Fragments arranged by size: 

                 220     210     208     198     136      59      52      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     959    1043    1275 
   Size:        959      84     232

  Fragments arranged by size: 

                 959     232      84
 
MfeI C'AATT_G

Cuts at:      0     193    1275 
   Size:        193    1082
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1275 
   Size:         40    1235
 
MscI TGG'CCA

Cuts at:      0     255    1275 
   Size:        255    1020
 
MslI CAynn'nnrTG

Cuts at:      0     866    1107    1163    1275 
   Size:        866     241      56     112

  Fragments arranged by size: 

                 866     241     112      56
 
MspA1I CmG'CkG

Cuts at:      0    1140    1275 
   Size:       1140     135
 
NaeI GCC'GGC

Cuts at:      0    1147    1275 
   Size:       1147     128
 
NdeI CA'TA_TG

Cuts at:      0    1104    1275 
   Size:       1104     171
 
NgoMIV G'CCGG_C

Cuts at:      0    1145    1275 
   Size:       1145     130
 
PpuMI rG'GwC_Cy

Cuts at:      0     108    1011    1275 
   Size:        108     903     264

  Fragments arranged by size: 

                 903     264     108
 
SanDI GG'GwC_CC

Cuts at:      0    1011    1275 
   Size:       1011     264
 
SmaI CCC'GGG

Cuts at:      0     454    1275 
   Size:        454     821
 
StyI C'CwwG_G

Cuts at:      0     342    1275 
   Size:        342     933
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     402    1275 
   Size:        402     873
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     199     231    1275 
   Size:        199      32    1044

  Fragments arranged by size: 

                1044     199      32
 
XbaI T'CTAG_A

Cuts at:      0     270    1275 
   Size:        270    1005
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     396    1275 
   Size:        396     879
 
XmaI C'CCGG_G

Cuts at:      0     452    1275 
   Size:        452     823
 
XmnI GAAnn'nnTTC

Cuts at:      0     625     961    1213    1275 
   Size:        625     336     252      62

  Fragments arranged by size: 

                 625     336     252      62
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApoI     AvaI     BanI    BanII     BbsI
    BbvCI     BcgI    BglII     BmrI     BplI     BpmI   Bpu10I    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrBI    BsrFI
   BstAPI    BstBI    BstYI     BtgI     BtsI    CspCI   DraIII     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV    PpuMI
    SanDI     SmaI     StyI    TaqII     TstI     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     NarI     NcoI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XhoI     ZraI
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