Restriction map for pF1KA1594
 (Circular) (Six-base) MAPSORT of: pF1KA1594.seq  Check: 1754  from: 1  to: 6050



pF1KA1594  6050 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:24  ..

AccI GT'mk_AC

Cuts at:   3088    3088 
   Size:       6050
 
Acc65I G'GTAC_C

Cuts at:   3066    3066 
   Size:       6050
 
AclI AA'CG_TT

Cuts at:   2079    5890    2079 
   Size:       3811    2239
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    139    2524    2741    2801    3875    4307    4660    5322     139 
   Size:       2385     217      60    1074     432     353     662     867

  Fragments arranged by size: 

                2385    1074     867     662     432     353     217      60
            
 
AflIII A'CryG_T

Cuts at:   3402    5202    5483    3402 
   Size:       1800     281    3969

  Fragments arranged by size: 

                3969    1800     281
 
AhdI GACnn_n'nnGTC

Cuts at:   3032    3032 
   Size:       6050
 
AleI CACnn'nnGTG

Cuts at:   3016    3016 
   Size:       6050
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1406    1539    1406 
   Size:        133    5917
 
AlwNI CAG_nnn'CTG

Cuts at:   1125    2550    2719    3355    4793    1125 
   Size:       1425     169     636    1438    2382

  Fragments arranged by size: 

                2382    1438    1425     636     169
 
ApaLI G'TGCA_C

Cuts at:   4888    4888 
   Size:       6050
 
ApoI r'AATT_y

Cuts at:    140     245    1241    1399    1488    1759    1974    2011    3054 
   Size:        105     996     158      89     271     215      37    1043

Cuts at:   3054    5403    5659     140 
   Size:       2349     256     531

  Fragments arranged by size: 

                2349    1043     996     531     271     256     215     158
                 105      89      37
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6050
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6050
 
AvaI C'yCGr_G

Cuts at:   3070    3070 
   Size:       6050
 
AvrII C'CTAG_G

Cuts at:   1774    1774 
   Size:       6050
 
BamHI G'GATC_C

Cuts at:    181     931    2406    3075     181 
   Size:        750    1475     669    3156

  Fragments arranged by size: 

                3156    1475     750     669
 
BanI G'GyrC_C

Cuts at:   3066    3480    3728    3763    3066 
   Size:        414     248      35    5353

  Fragments arranged by size: 

                5353     414     248      35
 
BanII G_rGCy'C

Cuts at:   2035    3064    4094    2035 
   Size:       1029    1030    3991

  Fragments arranged by size: 

                3991    1030    1029
 
BbeI G_GCGC'C

Cuts at:   3484    3732    3484 
   Size:        248    5802
 
BbsI GAAGACnn'nnnn_

Cuts at:   1468    2144    2620    3031    1468 
   Size:        676     476     411    4487

  Fragments arranged by size: 

                4487     676     476     411
 
BbvCI CC'TCA_GC

Cuts at:   1025    2092    2726    1025 
   Size:       1067     634    4349

  Fragments arranged by size: 

                4349    1067     634
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2902    2936    2902 
   Size:         34    6016
 
BciVI GTATCCnnnnn_n'

Cuts at:   1237    3948    5004    6050    1237 
   Size:       2711    1056    1046    1237

  Fragments arranged by size: 

                2711    1237    1056    1046
 
BclI T'GATC_A

Cuts at:   3570    3570 
   Size:       6050
 
BfrBI ATG'CAT

Cuts at:   2659    2659 
   Size:       6050
 
BglII A'GATC_T

Cuts at:   2963    3565    5602    2963 
   Size:        602    2037    3411

  Fragments arranged by size: 

                3411    2037     602
 
BlpI GC'TnA_GC

Cuts at:   3162    3162 
   Size:       6050
 
Bme1580I G_kGCm'C

Cuts at:   1100    3675    3768    4892    1100 
   Size:       2575      93    1124    2258

  Fragments arranged by size: 

                2575    2258    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    167     293    3262    3343    3678    5822     167 
   Size:        126    2969      81     335    2144     395

  Fragments arranged by size: 

                2969    2144     395     335     126      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1386    4417    1386 
   Size:       3031    3019
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    260     798    1177    1499    1729    2450    2482    2902     260 
   Size:        538     379     322     230     721      32     420    3408

  Fragments arranged by size: 

                3408     721     538     420     379     322     230      32
            
 
Bpu10I CC'TnA_GC

Cuts at:   1025    2092    2726    1025 
   Size:       1067     634    4349

  Fragments arranged by size: 

                4349    1067     634
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1312    3226    4105    4819    5117    1312 
   Size:       1914     879     714     298    2245

  Fragments arranged by size: 

                2245    1914     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1303    5754    1303 
   Size:       4451    1599
 
BsaAI yAC'GTr

Cuts at:   3403    3403 
   Size:       6050
 
BsaBI GATnn'nnATC

Cuts at:      6    2924    3564    5601       6 
   Size:       2918     640    2037     455

  Fragments arranged by size: 

                2918    2037     640     455
 
BsaHI Gr'CG_yC

Cuts at:   3481    3729    4431    5927    3481 
   Size:        248     702    1496    3604

  Fragments arranged by size: 

                3604    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2320    3239    3760    4438    4849    4996    5270    2320 
   Size:        919     521     678     411     147     274    3100

  Fragments arranged by size: 

                3100     919     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    104    1649     104 
   Size:       1545    4505
 
BseYI C'CCAG_C

Cuts at:   3475    4233    4898    5306    3475 
   Size:        758     665     408    4219

  Fragments arranged by size: 

                4219     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2932    2932 
   Size:       6050
 
BsiEI CG_ry'CG

Cuts at:    104    3592    3638    4868     104 
   Size:       3488      46    1230    1286

  Fragments arranged by size: 

                3488    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1746    2035    3064    3842    4032    4892    1746 
   Size:        289    1029     778     190     860    2904

  Fragments arranged by size: 

                2904    1029     860     778     289     190
 
BsmI GAATG_Cn'

Cuts at:   1170    1170 
   Size:       6050
 
BsmBI CGTCTCn'nnnn_

Cuts at:    452     452 
   Size:       6050
 
Bsp1286I G_dGCh'C

Cuts at:   1100    1746    2035    3064    3675    3768    3842    4032    4094 
   Size:        646     289    1029     611      93      74     190      62

Cuts at:   4094    4892    1100 
   Size:        798    2258

  Fragments arranged by size: 

                2258    1029     798     646     611     289     190      93
                  74      62
 
BspHI T'CATG_A

Cuts at:   1395    2283    4482    1395 
   Size:        888    2199    2963

  Fragments arranged by size: 

                2963    2199     888
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1162    3100    3314    3616    3997    1162 
   Size:       1938     214     302     381    3215

  Fragments arranged by size: 

                3215    1938     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    4342    4396    5880    6045      50 
   Size:       4292      54    1484     165      55

  Fragments arranged by size: 

                4292    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1884    3962    1884 
   Size:       2078    3972
 
BsrFI r'CCGG_y

Cuts at:   2774    4048    4229    2774 
   Size:       1274     181    4595

  Fragments arranged by size: 

                4595    1274     181
 
BssSI C'ACGA_G

Cuts at:   4321    5029    4321 
   Size:        708    5342
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1125    3427    1125 
   Size:       2302    3748
 
BstBI TT'CG_AA

Cuts at:    144     585    4411     144 
   Size:        441    3826    1783

  Fragments arranged by size: 

                3826    1783     441
 
BstYI r'GATC_y

Cuts at:      1     181     807     931    2406    2473    2963    3075    3542 
   Size:        180     626     124    1475      67     490     112     467

Cuts at:   3542    3565    3900    4146    4452    4464    4550    5602       1 
   Size:         23     335     246     306      12      86    1052     449

  Fragments arranged by size: 

                1475    1052     626     490     467     449     335     306
                 246     180     124     112      86      67      23      12
            
 
Bsu36I CC'TnA_GG

Cuts at:    767     767 
   Size:       6050
 
BtgI C'CryG_G

Cuts at:   1231    1231 
   Size:       6050
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4000    4181    4344    4000 
   Size:        181     163    5706

  Fragments arranged by size: 

                5706     181     163
 
BtsI GCAGTG_nn'

Cuts at:   3293    3293 
   Size:       6050
 
ClaI AT'CG_AT

Cuts at:    616     616 
   Size:       6050
 
DraI TTT'AAA

Cuts at:   3049    3049 
   Size:       6050
 
DrdI GACnn_nn'nnGTC

Cuts at:   3756    5100    3756 
   Size:       1344    4706
 
EaeI y'GGCC_r

Cuts at:   3635    3809    4200    4227    5572    3635 
   Size:        174     391      27    1345    4113

  Fragments arranged by size: 

                4113    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3635    3635 
   Size:       6050
 
EarI CTCTTCn'nnn_

Cuts at:    863     951    2219    2522    4073    4283     863 
   Size:         88    1268     303    1551     210    2630

  Fragments arranged by size: 

                2630    1551    1268     303     210      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4258    5000    5146    5860    4258 
   Size:        742     146     714    4448

  Fragments arranged by size: 

                4448     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2033    3062    2033 
   Size:       1029    5021
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     260     798    1177    1499    1729    2450    2482    2524 
   Size:        121     538     379     322     230     721      32      42

Cuts at:   2524    2741    2801    2902    3875    4307    4660    5322     139 
   Size:        217      60     101     973     432     353     662     867

  Fragments arranged by size: 

                 973     867     721     662     538     432     379     353
                 322     230     217     121     101      60      42      32
            
 
EcoO109I rG'GnC_Cy

Cuts at:    488    1779    3189     488 
   Size:       1291    1410    3349

  Fragments arranged by size: 

                3349    1410    1291
 
EcoRI G'AATT_C

Cuts at:    140     245    1974    3054     140 
   Size:        105    1729    1080    3136

  Fragments arranged by size: 

                3136    1729    1080     105
 
EcoRV GAT'ATC

Cuts at:    734    1296     734 
   Size:        562    5488
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1696    2052    2245    2982    4069    1696 
   Size:        356     193     737    1087    3677

  Fragments arranged by size: 

                3677    1087     737     356     193
 
FspI TGC'GCA

Cuts at:   1931    3831    1931 
   Size:       1900    4150
 
FspAI rTGC'GCAy

Cuts at:   1931    1931 
   Size:       6050
 
HaeII r_GCGC'y

Cuts at:   3484    3732    4962    5733    3484 
   Size:        248    1230     771    3801

  Fragments arranged by size: 

                3801    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    114     146     769     801    1577    1609    2662    2694    2742 
   Size:         32     623      32     776      32    1053      32      48

Cuts at:   2742    2774    3018    3050    3892    3922    3924    3954     114 
   Size:         32     244      32     842      30       2      30    2210

  Fragments arranged by size: 

                2210    1053     842     776     623     244      48      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    329    3089     329 
   Size:       2760    3290
 
HpaI GTT'AAC

Cuts at:    329     329 
   Size:       6050
 
KasI G'GCGC_C

Cuts at:   3480    3728    3480 
   Size:        248    5802
 
KpnI G_GTAC'C

Cuts at:   3070    3070 
   Size:       6050
 
MluI A'CGCG_T

Cuts at:   5483    5483 
   Size:       6050
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1903    4809    4993    1903 
   Size:       2906     184    2960

  Fragments arranged by size: 

                2960    2906     184
 
MscI TGG'CCA

Cuts at:   3811    3811 
   Size:       6050
 
MslI CAynn'nnrTG

Cuts at:   2288    3016    4039    4166    2288 
   Size:        728    1023     127    4172

  Fragments arranged by size: 

                4172    1023     728     127
 
MspA1I CmG'CkG

Cuts at:    849    1425    2239    3161    3475    3835    4619    4864     849 
   Size:        576     814     922     314     360     784     245    2035

  Fragments arranged by size: 

                2035     922     814     784     576     360     314     245
            
 
NaeI GCC'GGC

Cuts at:   4231    4231 
   Size:       6050
 
NarI GG'CG_CC

Cuts at:   3481    3729    3481 
   Size:        248    5802
 
NcoI C'CATG_G

Cuts at:   1231    1231 
   Size:       6050
 
NdeI CA'TA_TG

Cuts at:   1934    1934 
   Size:       6050
 
NgoMIV G'CCGG_C

Cuts at:   4229    4229 
   Size:       6050
 
NsiI A_TGCA'T

Cuts at:   2661    2661 
   Size:       6050
 
NspI r_CATG'y

Cuts at:   1583    3103    3603    5206    1583 
   Size:       1520     500    1603    2427

  Fragments arranged by size: 

                2427    1603    1520     500
 
PciI A'CATG_T

Cuts at:   5202    5202 
   Size:       6050
 
PmeI GTTT'AAAC

Cuts at:   3049    3049 
   Size:       6050
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4496    4528    4496 
   Size:         32    6018
 
PpuMI rG'GwC_Cy

Cuts at:    488    1779     488 
   Size:       1291    4759
 
PstI C_TGCA'G

Cuts at:    435    1130    3097    3782     435 
   Size:        695    1967     685    2703

  Fragments arranged by size: 

                2703    1967     695     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6050
 
PvuII CAG'CTG

Cuts at:    849    1425    2239    3475    3835     849 
   Size:        576     814    1236     360    3064

  Fragments arranged by size: 

                3064    1236     814     576     360
 
RsrII CG'GwC_CG

Cuts at:   4245    4245 
   Size:       6050
 
SacI G_AGCT'C

Cuts at:   2035    3064    2035 
   Size:       1029    5021
 
SalI G'TCGA_C

Cuts at:   3087    3087 
   Size:       6050
 
SapI GCTCTTCn'nnn_

Cuts at:   2219    2522    4073    4283    2219 
   Size:        303    1551     210    3986

  Fragments arranged by size: 

                3986    1551     303     210
 
SbfI CC_TGCA'GG

Cuts at:   3097    3097 
   Size:       6050
 
ScaI AGT'ACT

Cuts at:   5567    5567 
   Size:       6050
 
SfcI C'TryA_G

Cuts at:     33     431     626    1126    1355    3093    3778    4746    4937 
   Size:        398     195     500     229    1738     685     968     191

Cuts at:   4937      33 
   Size:       1146

  Fragments arranged by size: 

                1738    1146     968     685     500     398     229     195
                 191
 
SfoI GGC'GCC

Cuts at:   3482    3730    3482 
   Size:        248    5802
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6050
 
SmaI CCC'GGG

Cuts at:   3072    3072 
   Size:       6050
 
SmlI C'TyrA_G

Cuts at:   1327    3205    4120    4834    5096    1327 
   Size:       1878     915     714     262    2281

  Fragments arranged by size: 

                2281    1878     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6050
 
SphI G_CATG'C

Cuts at:   3103    3603    3103 
   Size:        500    5550
 
StuI AGG'CCT

Cuts at:   3040    3040 
   Size:       6050
 
StyI C'CwwG_G

Cuts at:    250     935    1231    1774    1917    2036    3184    3538     250 
   Size:        685     296     543     143     119    1148     354    2762

  Fragments arranged by size: 

                2762    1148     685     543     354     296     143     119
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3633    3766    4225    4434    3633 
   Size:        133     459     209    5249

  Fragments arranged by size: 

                5249     459     209     133
 
TatI w'GTAC_w

Cuts at:   2708    5565    2708 
   Size:       2857    3193
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5567    5599    5567 
   Size:         32    6018
 
Tth111I GACn'n_nGTC

Cuts at:   1875    3847    1875 
   Size:       1972    4078
 
XbaI T'CTAG_A

Cuts at:    402    1945    3081     402 
   Size:       1543    1136    3371

  Fragments arranged by size: 

                3371    1543    1136
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1008    1072    2034    3545    1008 
   Size:         64     962    1511    3513

  Fragments arranged by size: 

                3513    1511     962      64
 
XmaI C'CCGG_G

Cuts at:   3070    3070 
   Size:       6050
 
XmnI GAAnn'nnTTC

Cuts at:   2228    2228 
   Size:       6050
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NsiI     NspI     PciI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AloI     ApaI     AscI
     BaeI     BglI    BmgBI     BmtI    BsaXI    BsiWI    BspEI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I    CspCI   DraIII    EcoNI     FseI
  HindIII     MfeI     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com