Restriction map for pF1KB6746
##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
TaqI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

BfaI          206  CTAG                 C^TAG
BsaAI         299  CACGTA               [CT]AC^GT[GA]
BsaJI          23  CCCCGG               C^C[ACGT][ACGT]GG
BsiEI          26  CGGCCG               CG[GA][CT]^CG
BsmFI          21  GTCCC                GGGAC(10/14)
Bsp1407I      285  TGTACA               T^GTACA
BsrGI         285  TGTACA               T^GTACA
BstNI          33  CCAGG                CC^[AT]GG
Cac8I           4  GCTTGC               GC[ACGT]^[ACGT]GC
DraI           66  TTTAAA               TTT^AAA
EaeI           26  CGGCCG               [CT]^GGCC[GA]
EagI           26  CGGCCG               C^GGCCG
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
EcoRII         33  CCAGG                ^CC[AT]GG
HaeIII         27  GGCC                 GG^CC
MaeI          206  CTAG                 C^TAG
MvaI           33  CCAGG                CC^[AT]GG
MwoI            4  GCTTGCGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI          242  GAGTC                GAGTC(4/5)
PshAI         376  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI          184  CTTGAG               C^T[CT][GA]AG
SspBI         285  TGTACA               T^GTACA
SwaI           65  ATTTAAAT             ATTT^AAAT
XbaI          205  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          15  CGCG                 CG^CG
               17  CGCG                
AciI           14  CCGC                 CCGC(-3/-1)
               18  GCGG                
AfaI          201  GTAC                 GT^AC
              286  GTAC                
AluI          135  AGCT                 AG^CT
              254  AGCT                
AvaII          20  GGTCC                G^G[AT]CC
              249  GGTCC               
BbsI           78  GAAGAC               GAAGAC(2/6)
              383  GTCTTC              
BbvI          322  GCAGC                GCAGC(8/12)
               10  GCTGC               
BcnI           24  CCCGG                CC^[GC]GG
              246  CCGGG               
BpuAI          78  GAAGAC               GAAGAC(2/6)
              383  GTCTTC              
BstUI          15  CGCG                 CG^CG
               17  CGCG                
CfoI            8  GCGC                 GCG^C
               16  GCGC                
Cfr13I         20  GGTCC                G^G[ACGT]CC
              249  GGTCC               
HapII          25  CCGG                 C^CGG
              246  CCGG                
HhaI            8  GCGC                 GCG^C
               16  GCGC                
HinP1I          8  GCGC                 G^CGC
               16  GCGC                
HpaII          25  CCGG                 C^CGG
              246  CCGG                
MaeII         176  ACGT                 A^CGT
              300  ACGT                
MseI           67  TTAA                 T^TAA
              173  TTAA                
MspI           25  CCGG                 C^CGG
              246  CCGG                
MvnI           15  CGCG                 CG^CG
               17  CGCG                
NciI           24  CCCGG                CC^[GC]GG
              246  CCGGG               
RsaI          201  GTAC                 GT^AC
              286  GTAC                
Sau96I         20  GGTCC                G^G[ACGT]CC
              249  GGTCC               
SfcI          131  CTACAG               C^T[GA][CT]AG
              137  CTACAG              
Tru9I          67  TTAA                 T^TAA
              173  TTAA                
TseI           10  GCTGC                G^C[AT]GC
              322  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

BssKI       Fnu4HI      HinfI       ItaI        MboII       
MnlI        NlaIV       ScrFI       TfiI        
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