Restriction map for pF1KE0550
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        SacI        SacII       
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          624  GTCGAC               GT^[AC][GT]AC
AccII         676  CGCG                 CG^CG
AfaI           66  GTAC                 GT^AC
AflIII         14  ACATGT               A^C[GA][CT]GT
AlwI          688  GGATC                GGATC(4/5)
BalI          356  TGGCCA               TGG^CCA
BbsI           40  GTCTTC               GAAGAC(2/6)
BbvI           71  GCTGC                GCAGC(8/12)
BcgI          863  GCAGATTCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          461  CCGGG                CC^[GC]GG
BfaI           91  CTAG                 C^TAG
BglII         494  AGATCT               A^GATCT
BpuAI          40  GTCTTC               GAAGAC(2/6)
BsaBI         471  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          517  GAGACC               GGTCTC(1/5)
BseRI         619  GAGGAG               GAGGAG(10/8)
BsmAI         517  GAGAC                GTCTC(1/5)
BsmFI         824  GTCCC                GGGAC(10/14)
BspLU11I       14  ACATGT               A^CATGT
BsrDI         896  CATTGC               GCAATG(2/0)
BstUI         676  CGCG                 CG^CG
CfoI          677  GCGC                 GCG^C
CpoI          221  CGGACCG              CG^G[AT]CCG
DraI          787  TTTAAA               TTT^AAA
DraII          96  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          356  TGGCCA               [CT]^GGCC[GA]
EarI          844  GAAGAG               CTCTTC(1/4)
EcoNI         324  CCTGGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       96  AGGGCCT              [GA]G^G[ACGT]CC[CT]
HapII         461  CCGG                 C^CGG
HhaI          677  GCGC                 GCG^C
HinP1I        677  GCGC                 G^CGC
HpaII         461  CCGG                 C^CGG
Ksp632I       844  GAAGAG               CTCTTC(1/4)
MaeI           91  CTAG                 C^TAG
MaeIII        554  GTGAC                ^GT[ACGT]AC
MamI          471  GATTGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         356  TGGCCA               TGG^CCA
MscI          356  TGGCCA               TGG^CCA
MslI          558  CATGAACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         69  CCGCTG               C[AC]G^C[GT]G
MspI          461  CCGG                 C^CGG
MvnI          676  CGCG                 CG^CG
MwoI           90  GCTAGCAGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          461  CCGGG                CC^[GC]GG
NheI           90  GCTAGC               G^CTAGC
NspI           14  ACATGT               [GA]CATG^[CT]
PleI          622  GAGTC                GAGTC(4/5)
RsaI           66  GTAC                 GT^AC
RsrII         221  CGGACCG              CG^G[AT]CCG
SalI          624  GTCGAC               G^TCGAC
TfiI          866  GATTC                G^A[AT]TC
TseI           71  GCTGC                G^C[AT]GC
Tsp45I        554  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AluI          665  AGCT                 AG^CT
              855  AGCT                
BanII         384  GAGCCC               G[GA]GC[CT]^C
              727  GAGCCC              
BmyI          384  GAGCCC               G[AGT]GC[ACT]^C
              727  GAGCCC              
Bsp1286I      384  GAGCCC               G[AGT]GC[ACT]^C
              727  GAGCCC              
BsrI          287  ACTGG                ACTGG(1/-1)
              702  ACTGG               
BstYI         494  AGATCT               [GA]^GATC[CT]
              688  GGATCT              
Cac8I          90  GCTAGC               GC[ACGT]^[ACGT]GC
              242  GCGTGC              
DpnII         495  GATC                 ^GATC
              689  GATC                
EcoRI         216  GAATTC               G^AATTC
              770  GAATTC              
Fnu4HI         71  GCTGC                GC^[ACGT]GC
              320  GCGGC               
HgaI          231  GACGC                GACGC(5/10)
              341  GCGTC               
HincII        343  GTCAAC               GT[CT]^[GA]AC
              624  GTCGAC              
HindII        343  GTCAAC               GT[CT]^[GA]AC
              624  GTCGAC              
HinfI         622  GAGTC                G^A[ACGT]TC
              866  GATTC               
HphI          634  GGTGA                GGTGA(8/7)
              404  TCACC               
ItaI           71  GCTGC                GC^[ACGT]GC
              320  GCGGC               
MaeII         563  ACGT                 A^CGT
              822  ACGT                
MboI          495  GATC                 ^GATC
              689  GATC                
MflI          494  AGATCT               [GA]^GATC[CT]
              688  GGATCT              
NdeII         495  GATC                 ^GATC
              689  GATC                
Sau3AI        495  GATC                 ^GATC
              689  GATC                
TaqI           20  TCGA                 T^CGA
              625  TCGA                
TthHB8I        20  TCGA                 T^CGA
              625  TCGA                
XhoII         494  AGATCT               [GA]^GATC[CT]
              688  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AcsI        ApoI        AvaII       BpmI        
BsaJI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        EcoRII      FokI        
HaeIII      MboII       MnlI        MseI        MvaI        
NlaIII      Sau96I      ScrFI       SfaNI       Tru9I       
Tsp509I     TspRI       
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