Restriction map for pF1KE0549
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TseI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          403  AAATTC               [GA]^AATT[CT]
AfaI          180  GTAC                 GT^AC
ApoI          403  AAATTC               [GA]^AATT[CT]
Asp700I        57  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         352  GAGCAC               G[AT]GC[AT]^C
AvaII         393  GGACC                G^G[AT]CC
BcgI          354  GCACCCGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          373  CTAG                 C^TAG
BglII         218  AGATCT               A^GATCT
BmyI          352  GAGCAC               G[AGT]GC[ACT]^C
BsiHKAI       352  GAGCAC               G[AT]GC[AT]^C
BsmFI         318  GTCCC                GGGAC(10/14)
BsmI          414  GAATGC               GAATGC(1/-1)
Bsp1286I      352  GAGCAC               G[AGT]GC[ACT]^C
BspMI         249  GCAGGT               ACCTGC(4/8)
BssKI          75  CCAGG                ^CC[ACGT]GG
BstNI          75  CCAGG                CC^[AT]GG
BstYI         218  AGATCT               [GA]^GATC[CT]
Cfr13I        393  GGACC                G^G[ACGT]CC
DraI          208  TTTAAA               TTT^AAA
EaeI            5  CGGCCA               [CT]^GGCC[GA]
EarI          421  GAAGAG               CTCTTC(1/4)
EcoRII         75  CCAGG                ^CC[AT]GG
FauI          357  CCCGC                CCCGC(4/6)
Fnu4HI          4  GCGGC                GC^[ACGT]GC
HaeIII          6  GGCC                 GG^CC
HinfI         155  GAATC                G^A[ACGT]TC
ItaI            4  GCGGC                GC^[ACGT]GC
Ksp632I       421  GAAGAG               CTCTTC(1/4)
MaeI          373  CTAG                 C^TAG
MaeIII         68  GTAAC                ^GT[ACGT]AC
MflI          218  AGATCT               [GA]^GATC[CT]
MseI          209  TTAA                 T^TAA
MvaI           75  CCAGG                CC^[AT]GG
MwoI          367  GCTGACCTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          134  CATATG               CA^TATG
NlaIV         145  GGAGCC               GG[ACGT]^[ACGT]CC
RsaI          180  GTAC                 GT^AC
Sau96I        393  GGACC                G^G[ACGT]CC
ScrFI          75  CCAGG                CC^[ACGT]GG
TfiI          155  GAATC                G^A[AT]TC
Tru9I         209  TTAA                 T^TAA
TspRI         329  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         218  AGATCT               [GA]^GATC[CT]
XmnI           57  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BstF5I         72  CATCC                GGATG(2/0)
               53  GGATG               
Cac8I           7  GCCAGC               GC[ACGT]^[ACGT]GC
              288  GCCAGC              
DdeI          350  CTGAG                C^T[ACGT]AG
              389  CTAAG               
DpnII         201  GATC                 ^GATC
              219  GATC                
FokI           72  CATCC                GGATG(9/13)
               53  GGATG               
HphI          333  GGTGA                GGTGA(8/7)
              225  TCACC               
MboI          201  GATC                 ^GATC
              219  GATC                
NdeII         201  GATC                 ^GATC
              219  GATC                
Sau3AI        201  GATC                 ^GATC
              219  GATC                
TaqI          158  TCGA                 T^CGA
              236  TCGA                
TthHB8I       158  TCGA                 T^CGA
              236  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AciI        AluI        BsiYI       BslI        BsrI        
MboII       MnlI        Tsp509I     
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