Restriction map for pF1KB6457
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BcgI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT14I     EcoT22I     FseI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeIII      MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tsp45I      Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          795  GTCTAC               GT^[AC][GT]AC
AclI           38  AACGTT               AA^CGTT
AcsI          690  GAATTC               [GA]^AATT[CT]
AlwI           70  GATCC                GGATC(4/5)
AocI          400  CCTGAGG              CC^T[ACGT]AGG
ApoI          690  GAATTC               [GA]^AATT[CT]
Asp700I       497  GAAGCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         413  GAGCAC               G[AT]GC[AT]^C
AviII          90  TGCGCA               TGC^GCA
BbvCI         695  CCTCAGC              CCTCAGC(-5/-2)
BciVI         201  GTATCC               GTATCC(6/5)
BclI          846  TGATCA               T^GATCA
BcnI          765  CCGGG                CC^[GC]GG
BsaBI         870  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI            4  GAGACC               GGTCTC(1/5)
BseRI         164  GAGGAG               GAGGAG(10/8)
BsiHKAI       413  GAGCAC               G[AT]GC[AT]^C
BsiWI         677  CGTACG               C^GTACG
BstXI         527  CCAAAGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          69  AGATCC               [GA]^GATC[CT]
Bsu36I        400  CCTGAGG              CC^T[ACGT]AGG
DraI          486  TTTAAA               TTT^AAA
EaeI          665  CGGCCA               [CT]^GGCC[GA]
Eco81I        400  CCTGAGG              CC^T[ACGT]AGG
EcoRI         690  GAATTC               G^AATTC
FauI            8  CCCGC                CCCGC(4/6)
FbaI          846  TGATCA               T^GATCA
FspI           90  TGCGCA               TGC^GCA
HaeII         761  AGCGCC               [GA]GCGC^[CT]
HindIII       549  AAGCTT               A^AGCTT
HphI          802  TCACC                GGTGA(8/7)
MaeII          39  ACGT                 A^CGT
MamI          870  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           69  AGATCC               [GA]^GATC[CT]
MslI           82  CATTACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        860  CAGCTG               C[AC]G^C[GT]G
NciI          765  CCGGG                CC^[GC]GG
Psp1406I       38  AACGTT               AA^CGTT
PvuII         860  CAGCTG               CAG^CTG
SapI          410  GAAGAGC              GCTCTTC(1/4)
ScaI          186  AGTACT               AGT^ACT
SfcI          728  CTGTAG               C^T[GA][CT]AG
TfiI          502  GATTC                G^A[AT]TC
TspRI         843  CACTG                CA[GC]TG[ACGT][ACGT]^
XbaI          387  TCTAGA               T^CTAGA
XcmI          840  CCACACTGATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          69  AGATCC               [GA]^GATC[CT]
XmnI          497  GAAGCGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

ApaI          139  GGGCCC               GGGCC^C
              767  GGGCCC              
BfaI          388  CTAG                 C^TAG
              539  CTAG                
BsaJI         654  CCAGGG               C^C[ACGT][ACGT]GG
              816  CCAGGG              
BsmAI           4  GAGAC                GTCTC(1/5)
              833  GAGAC               
BsrI          534  ACTGG                ACTGG(1/-1)
              668  CCAGT               
BstNI         654  CCAGG                CC^[AT]GG
              816  CCAGG               
CfoI           91  GCGC                 GCG^C
              762  GCGC                
DraII         138  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              139  GGGCCCC             
EarI           21  GAAGAG               CTCTTC(1/4)
              410  GAAGAG              
EcoO109I      138  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              139  GGGCCCC             
EcoRII        654  CCAGG                ^CC[AT]GG
              816  CCAGG               
HapII          28  CCGG                 C^CGG
              765  CCGG                
HhaI           91  GCGC                 GCG^C
              762  GCGC                
HinP1I         91  GCGC                 G^CGC
              762  GCGC                
HpaII          28  CCGG                 C^CGG
              765  CCGG                
Ksp632I        21  GAAGAG               CTCTTC(1/4)
              410  GAAGAG              
MaeI          388  CTAG                 C^TAG
              539  CTAG                
MboII          21  GAAGA                GAAGA(8/7)
              410  GAAGA               
MspI           28  CCGG                 C^CGG
              765  CCGG                
MvaI          654  CCAGG                CC^[AT]GG
              816  CCAGG               
SfaNI         278  GCATC                GCATC(5/9)
              703  GCATC               
TaqI          246  TCGA                 T^CGA
              756  TCGA                
TthHB8I       246  TCGA                 T^CGA
              756  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AfaI        AluI        BanII       BbvI        
BmyI        BsiYI       BslI        Bsp1286I    BssKI       
BstF5I      Cfr13I      DdeI        DpnII       Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MboI        
MnlI        MseI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       Tru9I       TseI        Tsp509I     
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