Restriction map for pF1KB6457
 (Linear) (Six-base) MAPSORT of: KIBB6457.seq  Check: 8438  from: 1  to: 954



KIBB6457.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     796     954 
   Size:        796     158
 
AclI AA'CG_TT

Cuts at:      0      39     954 
   Size:         39     915
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     954 
   Size:         40     914
 
ApaI G_GGCC'C

Cuts at:      0     143     771     954 
   Size:        143     628     183

  Fragments arranged by size: 

                 628     183     143
 
ApoI r'AATT_y

Cuts at:      0     690     954 
   Size:        690     264
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     184     217     954 
   Size:        184      33     737

  Fragments arranged by size: 

                 737     184      33
 
BanII G_rGCy'C

Cuts at:      0     143     754     771     954 
   Size:        143     611      17     183

  Fragments arranged by size: 

                 611     183     143      17
 
BbvCI CC'TCA_GC

Cuts at:      0     696     954 
   Size:        696     258
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     212     954 
   Size:        212     742
 
BclI T'GATC_A

Cuts at:      0     846     954 
   Size:        846     108
 
Bme1580I G_kGCm'C

Cuts at:      0     143     771     954 
   Size:        143     628     183

  Fragments arranged by size: 

                 628     183     143
 
Bpu10I CC'TnA_GC

Cuts at:      0     696     954 
   Size:        696     258
 
BsaBI GATnn'nnATC

Cuts at:      0     874     954 
   Size:        874      80
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     179     954 
   Size:        179     775
 
BseYI C'CCAG_C

Cuts at:      0     770     954 
   Size:        770     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     417     954 
   Size:        417     537
 
BsiWI C'GTAC_G

Cuts at:      0     677     954 
   Size:        677     277
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     417     754     771     954 
   Size:        143     274     337      17     183

  Fragments arranged by size: 

                 337     274     183     143      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     534     954 
   Size:        534     420
 
BstYI r'GATC_y

Cuts at:      0      69     954 
   Size:         69     885
 
Bsu36I CC'TnA_GG

Cuts at:      0     401     954 
   Size:        401     553
 
DraI TTT'AAA

Cuts at:      0     488     954 
   Size:        488     466
 
EaeI y'GGCC_r

Cuts at:      0     665     954 
   Size:        665     289
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     405     954 
   Size:         16     389     549

  Fragments arranged by size: 

                 549     389      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     195     954 
   Size:        195     759
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     954 
   Size:         40     914
 
EcoO109I rG'GnC_Cy

Cuts at:      0     139     140     954 
   Size:        139       1     814

  Fragments arranged by size: 

                 814     139       1
 
EcoRI G'AATT_C

Cuts at:      0     690     954 
   Size:        690     264
 
FspI TGC'GCA

Cuts at:      0      92     954 
   Size:         92     862
 
HaeII r_GCGC'y

Cuts at:      0     765     954 
   Size:        765     189
 
HindIII A'AGCT_T

Cuts at:      0     549     954 
   Size:        549     405
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     933     954 
   Size:         97     836      21

  Fragments arranged by size: 

                 836      97      21
 
MslI CAynn'nnrTG

Cuts at:      0      86     954 
   Size:         86     868
 
MspA1I CmG'CkG

Cuts at:      0     862     954 
   Size:        862      92
 
PspOMI G'GGCC_C

Cuts at:      0     139     767     954 
   Size:        139     628     187

  Fragments arranged by size: 

                 628     187     139
 
PvuII CAG'CTG

Cuts at:      0     862     954 
   Size:        862      92
 
SapI GCTCTTCn'nnn_

Cuts at:      0     405     954 
   Size:        405     549
 
ScaI AGT'ACT

Cuts at:      0     188     954 
   Size:        188     766
 
SfcI C'TryA_G

Cuts at:      0     728     954 
   Size:        728     226
 
TatI w'GTAC_w

Cuts at:      0     186     670     864     954 
   Size:        186     484     194      90

  Fragments arranged by size: 

                 484     194     186      90
 
XbaI T'CTAG_A

Cuts at:      0     387     954 
   Size:        387     567
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     847     954 
   Size:        847     107
 
XmnI GAAnn'nnTTC

Cuts at:      0     501     954 
   Size:        501     453
 

 Enzymes that do cut:

     AccI     AclI     AcuI     ApaI     ApoI     BaeI    BanII    BbvCI
    BciVI     BclI Bme1580I   Bpu10I    BsaBI    BseRI    BseYI  BsiHKAI
    BsiWI Bsp1286I    BstXI    BstYI   Bsu36I     DraI     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII  HindIII     MmeI
     MslI   MspA1I   PspOMI    PvuII     SapI     ScaI     SfcI     TatI
     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI     BbeI     BbsI     BcgI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI
    EcoNI    EcoRV     FalI     FseI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XhoI     XmaI
     ZraI
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