Restriction map for pF1KB7646
 (Circular) (Six-base) MAPSORT of: pF1KB7646.seq  Check: 6119  from: 1  to: 4283



pF1KB7646  4283 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AccI GT'mk_AC

Cuts at:   1321    1321 
   Size:       4283
 
Acc65I G'GTAC_C

Cuts at:   1299    1299 
   Size:       4283
 
AclI AA'CG_TT

Cuts at:   4123    4123 
   Size:       4283
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1207    2108    2540    3287    3555    1207 
   Size:        901     432     747     268    1935

  Fragments arranged by size: 

                1935     901     747     432     268
 
AflIII A'CryG_T

Cuts at:   1635    2739    3716    1635 
   Size:       1104     977    2202

  Fragments arranged by size: 

                2202    1104     977
 
AgeI A'CCGG_T

Cuts at:   2671    2671 
   Size:       4283
 
AhdI GACnn_n'nnGTC

Cuts at:   1259    1259 
   Size:       4283
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    434     434 
   Size:       4283
 
AlwNI CAG_nnn'CTG

Cuts at:    915    1092    1588    3155     915 
   Size:        177     496    1567    2043

  Fragments arranged by size: 

                2043    1567     496     177
 
ApaLI G'TGCA_C

Cuts at:   3053    3053 
   Size:       4283
 
ApoI r'AATT_y

Cuts at:   1287    3636    3892    1287 
   Size:       2349     256    1678

  Fragments arranged by size: 

                2349    1678     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4283
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4283
 
AvaI C'yCGr_G

Cuts at:   1303    1303 
   Size:       4283
 
BamHI G'GATC_C

Cuts at:   1308    1308 
   Size:       4283
 
BanI G'GyrC_C

Cuts at:   1299    1713    1961    1996    1299 
   Size:        414     248      35    3586

  Fragments arranged by size: 

                3586     414     248      35
 
BanII G_rGCy'C

Cuts at:    866    1297    2327     866 
   Size:        431    1030    2822

  Fragments arranged by size: 

                2822    1030     431
 
BbeI G_GCGC'C

Cuts at:   1717    1965    1717 
   Size:        248    4035
 
BbsI GAAGACnn'nnnn_

Cuts at:    522     896    1130    1173    1192     522 
   Size:        374     234      43      19    3613

  Fragments arranged by size: 

                3613     374     234      43      19
 
BbvCI CC'TCA_GC

Cuts at:   1225    1225 
   Size:       4283
 
BciVI GTATCCnnnnn_n'

Cuts at:    631    2181    2942    4283     631 
   Size:       1550     761    1341     631

  Fragments arranged by size: 

                1550    1341     761     631
 
BclI T'GATC_A

Cuts at:   1803    1803 
   Size:       4283
 
BglII A'GATC_T

Cuts at:    207    1798    3835     207 
   Size:       1591    2037     655

  Fragments arranged by size: 

                2037    1591     655
 
BlpI GC'TnA_GC

Cuts at:   1395    1395 
   Size:       4283
 
Bme1580I G_kGCm'C

Cuts at:    855    1233    1908    2001    3057     855 
   Size:        378     675      93    1056    2081

  Fragments arranged by size: 

                2081    1056     675     378      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    647     746    1495    1576    1911    4055     647 
   Size:         99     749      81     335    2144     875

  Fragments arranged by size: 

                2144     875     749     335      99      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    811    2650     811 
   Size:       1839    2444
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    509     509 
   Size:       4283
 
Bpu10I CC'TnA_GC

Cuts at:   1225    1225 
   Size:       4283
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    115    1173    1459    2338    2830    3128     115 
   Size:       1058     286     879     492     298    1270

  Fragments arranged by size: 

                1270    1058     879     492     298     286
 
BsaI GGTCTCn'nnnn_

Cuts at:    155    1267    3987     155 
   Size:       1112    2720     451

  Fragments arranged by size: 

                2720    1112     451
 
BsaAI yAC'GTr

Cuts at:    175    1636     175 
   Size:       1461    2822
 
BsaBI GATnn'nnATC

Cuts at:      6    1797    3834       6 
   Size:       1791    2037     455

  Fragments arranged by size: 

                2037    1791     455
 
BsaHI Gr'CG_yC

Cuts at:   1714    1962    2664    4160    1714 
   Size:        248     702    1496    1837

  Fragments arranged by size: 

                1837    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    634    1472    1993    2671    2945    3092    3503     634 
   Size:        838     521     678     274     147     411    1414

  Fragments arranged by size: 

                1414     838     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1708    2466    3043    3539    1708 
   Size:        758     577     496    2452

  Fragments arranged by size: 

                2452     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1825    1871    3079     104 
   Size:       1721      46    1208    1308

  Fragments arranged by size: 

                1721    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    866    1297    2075    2265    3057     866 
   Size:        431     778     190     792    2092

  Fragments arranged by size: 

                2092     792     778     431     190
 
Bsp1286I G_dGCh'C

Cuts at:    855     866    1233    1297    1908    2001    2075    2265    2327 
   Size:         11     367      64     611      93      74     190      62

Cuts at:   2327    3057     855 
   Size:        730    2081

  Fragments arranged by size: 

                2081     730     611     367     190      93      74      64
                  62      11
 
BspEI T'CCGG_A

Cuts at:    634    3503     634 
   Size:       2869    1414
 
BspHI T'CATG_A

Cuts at:    497    3459     497 
   Size:       2962    1321
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1333    1547    1849    2230    1333 
   Size:        214     302     381    3386

  Fragments arranged by size: 

                3386     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2575    2629    4113    4278      50 
   Size:       2525      54    1484     165      55

  Fragments arranged by size: 

                2525    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2195    2195 
   Size:       4283
 
BsrFI r'CCGG_y

Cuts at:   1258    2281    2462    2671    1258 
   Size:       1023     181     209    2870

  Fragments arranged by size: 

                2870    1023     209     181
 
BssSI C'ACGA_G

Cuts at:   2554    2912    2554 
   Size:        358    3925
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1660    1660 
   Size:       4283
 
BstBI TT'CG_AA

Cuts at:   2644    2644 
   Size:       4283
 
BstEII G'GTnAC_C

Cuts at:    658     904     927     658 
   Size:        246      23    4014

  Fragments arranged by size: 

                4014     246      23
 
BstYI r'GATC_y

Cuts at:      1     207    1088    1308    1775    1798    2133    2379    3391 
   Size:        206     881     220     467      23     335     246    1012

Cuts at:   3391    3477    3489    3835       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     881     467     449     346     335     246     220
                 206      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4283
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2233    2414    2577    2233 
   Size:        181     163    3939

  Fragments arranged by size: 

                3939     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1526    1526 
   Size:       4283
 
ClaI AT'CG_AT

Cuts at:   1049    1049 
   Size:       4283
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    636     671     636 
   Size:         35    4248
 
DraI TTT'AAA

Cuts at:   1282    1282 
   Size:       4283
 
DraIII CAC_nnn'GTG

Cuts at:    685     685 
   Size:       4283
 
DrdI GACnn_nn'nnGTC

Cuts at:   1177    1989    2847    1177 
   Size:        812     858    2613

  Fragments arranged by size: 

                2613     858     812
 
EaeI y'GGCC_r

Cuts at:   1868    2042    2433    2460    3805    1868 
   Size:        174     391      27    1345    2346

  Fragments arranged by size: 

                2346    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1868    1868 
   Size:       4283
 
EarI CTCTTCn'nnn_

Cuts at:    914    2306    2516     914 
   Size:       1392     210    2681

  Fragments arranged by size: 

                2681    1392     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    548     843    2491    2801    2947    4093     548 
   Size:        295    1648     310     146    1146     738

  Fragments arranged by size: 

                1648    1146     738     310     295     146
 
EcoICRI GAG'CTC

Cuts at:    864    1295     864 
   Size:        431    3852
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    509    1207    2108    2540    3287    3555     509 
   Size:        698     901     432     747     268    1237

  Fragments arranged by size: 

                1237     901     747     698     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    296     898    1155     296 
   Size:        602     257    3424

  Fragments arranged by size: 

                3424     602     257
 
EcoO109I rG'GnC_Cy

Cuts at:   1422    1422 
   Size:       4283
 
EcoRI G'AATT_C

Cuts at:   1287    1287 
   Size:       4283
 
EcoRV GAT'ATC

Cuts at:   1047    1047 
   Size:       4283
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2302    2302 
   Size:       4283
 
FspI TGC'GCA

Cuts at:   2064    2064 
   Size:       4283
 
HaeII r_GCGC'y

Cuts at:   1717    1965    2987    3966    1717 
   Size:        248    1022     979    2034

  Fragments arranged by size: 

                2034    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1080    1112    1245    1277    2125    2155    2157    2187    1080 
   Size:         32     133      32     848      30       2      30    3176

  Fragments arranged by size: 

                3176     848     133      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1322    1322 
   Size:       4283
 
KasI G'GCGC_C

Cuts at:   1713    1961    1713 
   Size:        248    4035
 
KpnI G_GTAC'C

Cuts at:   1303    1303 
   Size:       4283
 
MluI A'CGCG_T

Cuts at:   3716    3716 
   Size:       4283
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:     98    2954    3138      98 
   Size:       2856     184    1243

  Fragments arranged by size: 

                2856    1243     184
 
MscI TGG'CCA

Cuts at:   2044    2044 
   Size:       4283
 
MslI CAynn'nnrTG

Cuts at:    112     416     502    2272    2399     112 
   Size:        304      86    1770     127    1996

  Fragments arranged by size: 

                1996    1770     304     127      86
 
MspA1I CmG'CkG

Cuts at:   1224    1394    1708    2068    3081    3326    1224 
   Size:        170     314     360    1013     245    2181

  Fragments arranged by size: 

                2181    1013     360     314     245     170
 
NaeI GCC'GGC

Cuts at:   2464    2464 
   Size:       4283
 
NarI GG'CG_CC

Cuts at:   1714    1962    1714 
   Size:        248    4035
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4283
 
NdeI CA'TA_TG

Cuts at:    231     231 
   Size:       4283
 
NgoMIV G'CCGG_C

Cuts at:   2462    2462 
   Size:       4283
 
NspI r_CATG'y

Cuts at:    415     600     609    1195    1336    1836    2743     415 
   Size:        185       9     586     141     500     907    1955

  Fragments arranged by size: 

                1955     907     586     500     185     141       9
 
PciI A'CATG_T

Cuts at:   2739    2739 
   Size:       4283
 
PflMI CCAn_nnn'nTGG

Cuts at:    685     915     685 
   Size:        230    4053
 
PmeI GTTT'AAAC

Cuts at:   1282    1282 
   Size:       4283
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3422    3454    3422 
   Size:         32    4251
 
PstI C_TGCA'G

Cuts at:   1330    2015    1330 
   Size:        685    3598
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4283
 
PvuII CAG'CTG

Cuts at:   1224    1708    2068    1224 
   Size:        484     360    3439

  Fragments arranged by size: 

                3439     484     360
 
RsrII CG'GwC_CG

Cuts at:   2478    2478 
   Size:       4283
 
SacI G_AGCT'C

Cuts at:    866    1297     866 
   Size:        431    3852
 
SalI G'TCGA_C

Cuts at:   1320    1320 
   Size:       4283
 
SapI GCTCTTCn'nnn_

Cuts at:   2306    2516    2306 
   Size:        210    4073
 
SbfI CC_TGCA'GG

Cuts at:   1330    1330 
   Size:       4283
 
ScaI AGT'ACT

Cuts at:   3800    3800 
   Size:       4283
 
SexAI A'CCwGG_T

Cuts at:    681     681 
   Size:       4283
 
SfcI C'TryA_G

Cuts at:     33    1326    2011    3004    3195      33 
   Size:       1293     685     993     191    1121

  Fragments arranged by size: 

                1293    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1715    1963    1715 
   Size:        248    4035
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4283
 
SmaI CCC'GGG

Cuts at:   1305    1305 
   Size:       4283
 
SmlI C'TyrA_G

Cuts at:    130    1152    1438    2353    2845    3107     130 
   Size:       1022     286     915     492     262    1306

  Fragments arranged by size: 

                1306    1022     915     492     286     262
 
SpeI A'CTAG_T

Cuts at:     67     382      67 
   Size:        315    3968
 
SphI G_CATG'C

Cuts at:    609    1336    1836     609 
   Size:        727     500    3056

  Fragments arranged by size: 

                3056     727     500
 
SspI AAT'ATT

Cuts at:    315     315 
   Size:       4283
 
StyI C'CwwG_G

Cuts at:    107    1417    1771     107 
   Size:       1310     354    2619

  Fragments arranged by size: 

                2619    1310     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    479    1866    1999    2458    2667     479 
   Size:       1387     133     459     209    2095

  Fragments arranged by size: 

                2095    1387     459     209     133
 
TatI w'GTAC_w

Cuts at:    149    3798     149 
   Size:       3649     634
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    732     764    3800    3832     732 
   Size:         32    3036      32    1183

  Fragments arranged by size: 

                3036    1183      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2080    2080 
   Size:       4283
 
XbaI T'CTAG_A

Cuts at:   1314    1314 
   Size:       4283
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    326    1778     326 
   Size:       1452    2831
 
XmaI C'CCGG_G

Cuts at:   1303    1303 
   Size:       4283
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI    PflMI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI    BsaXI
    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI
  BstZ17I   Bsu36I     FseI    FspAI  HindIII     HpaI     MfeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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