Restriction map for pF1KE0127
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        Alw44I      
AlwNI       Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcnI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
Hin1I       HpaI        KasI        Ksp632I     KspI        
MamI        MfeI        MflI        MluI        MroI        
MspA1I      MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I          5  GGTACC               G^GTACC
AciI          240  CCGC                 CCGC(-3/-1)
AcsI          862  GAATTT               [GA]^AATT[CT]
AocI          856  CCTGAGG              CC^T[ACGT]AGG
ApoI          862  GAATTT               [GA]^AATT[CT]
Asp718I         5  GGTACC               G^GTACC
AspHI         598  GAGCAC               G[AT]GC[AT]^C
BalI          358  TGGCCA               TGG^CCA
BanII         748  GAGCCC               G[GA]GC[CT]^C
BcgI          229  CGAGACCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         285  GGATAC               GTATCC(6/5)
BclI          989  TGATCA               T^GATCA
BlpI          242  GCTCAGC              GC^T[ACGT]AGC
BpmI          681  CTCCAG               CTGGAG(16/14)
Bpu1102I      242  GCTCAGC              GC^T[ACGT]AGC
BsaJI         967  CCATGG               C^C[ACGT][ACGT]GG
BsiHKAI       598  GAGCAC               G[AT]GC[AT]^C
BsiYI         644  CCATTTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          644  CCATTTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI        1089  CGTCTC               CGTCTC(1/5)
BsrBI         240  CCGCTC               CCGCTC(-3/-3)
BssKI         282  CCTGG                ^CC[ACGT]GG
BstNI         282  CCTGG                CC^[AT]GG
Bsu36I        856  CCTGAGG              CC^T[ACGT]AGG
CelII         242  GCTCAGC              GC^T[ACGT]AGC
DsaI          967  CCATGG               C^C[GA][CT]GG
EaeI          358  TGGCCA               [CT]^GGCC[GA]
Eco81I        856  CCTGAGG              CC^T[ACGT]AGG
EcoRII        282  CCTGG                ^CC[AT]GG
EcoT14I       967  CCATGG               C^C[AT][AT]GG
FbaI          989  TGATCA               T^GATCA
HapII          70  CCGG                 C^CGG
HincII       1021  GTCAAC               GT[CT]^[GA]AC
HindII       1021  GTCAAC               GT[CT]^[GA]AC
HindIII        96  AAGCTT               A^AGCTT
HpaII          70  CCGG                 C^CGG
KpnI            5  GGTACC               GGTAC^C
MaeII         218  ACGT                 A^CGT
MluNI         358  TGGCCA               TGG^CCA
MscI          358  TGGCCA               TGG^CCA
MspI           70  CCGG                 C^CGG
MvaI          282  CCTGG                CC^[AT]GG
MwoI          885  GCCATTGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          967  CCATGG               C^CATGG
NheI          708  GCTAGC               G^CTAGC
ScrFI         282  CCTGG                CC^[ACGT]GG
SmlI          593  CTCAAG               C^T[CT][GA]AG
SspI         1068  AATATT               AAT^ATT
StyI          967  CCATGG               C^C[AT][AT]GG
TaqI          228  TCGA                 T^CGA
TfiI          821  GATTC                G^A[AT]TC
TthHB8I       228  TCGA                 T^CGA
XcmI          704  CCAAGCTAGCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AhdI          321  GACCATTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1074  GACTTATCGTC         
AlwI          623  GGATC                GGATC(4/5)
              717  GGATC               
AspEI         321  GACCATTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1074  GACTTATCGTC         
BmyI          598  GAGCAC               G[AGT]GC[ACT]^C
              748  GAGCCC              
BsaI          230  GAGACC               GGTCTC(1/5)
              337  GAGACC              
Bsp1286I      598  GAGCAC               G[AGT]GC[ACT]^C
              748  GAGCCC              
BstXI         323  CCATTCGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              930  CCACATGATTGG        
CfoI          207  GCGC                 GCG^C
              276  GCGC                
Cfr13I         72  GGCCC                G^G[ACGT]CC
              946  GGGCC               
Eam1105I      321  GACCATTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1074  GACTTATCGTC         
HhaI          207  GCGC                 GCG^C
              276  GCGC                
HinP1I        207  GCGC                 G^CGC
              276  GCGC                
MslI          759  CATTCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1043  CACTCCAATG          
PleI          267  GACTC                GAGTC(4/5)
               59  GAGTC               
Sau96I         72  GGCCC                G^G[ACGT]CC
              946  GGGCC               
SfaNI         204  GATGC                GCATC(5/9)
              247  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AfaI        AluI        BanI        BbvI        BfaI        
BseRI       BsmAI       BsmFI       BsmI        BsrI        
BstF5I      Cac8I       DdeI        DpnII       Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com