Restriction map for pF1KE0127
 (Circular) (Six-base) MAPSORT of: pF1KE0127.seq  Check: 2129  from: 1  to: 4241



pF1KE0127  4241 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:   1279    1279 
   Size:       4241
 
Acc65I G'GTAC_C

Cuts at:    113    1257     113 
   Size:       1144    3097
 
AclI AA'CG_TT

Cuts at:   4081    4081 
   Size:       4241
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    130     178     658     764    2066    2498    3245    3513     130 
   Size:         48     480     106    1302     432     747     268     858

  Fragments arranged by size: 

                1302     858     747     480     432     268     106      48
            
 
AflIII A'CryG_T

Cuts at:   1593    2697    3674    1593 
   Size:       1104     977    2160

  Fragments arranged by size: 

                2160    1104     977
 
AgeI A'CCGG_T

Cuts at:   2629    2629 
   Size:       4241
 
AhdI GACnn_n'nnGTC

Cuts at:    434    1187     434 
   Size:        753    3488
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    931     931 
   Size:       4241
 
AlwNI CAG_nnn'CTG

Cuts at:   1546    3113    1546 
   Size:       1567    2674
 
ApaLI G'TGCA_C

Cuts at:   3011    3011 
   Size:       4241
 
ApoI r'AATT_y

Cuts at:    970    1245    3594    3850     970 
   Size:        275    2349     256    1361

  Fragments arranged by size: 

                2349    1361     275     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4241
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4241
 
AvaI C'yCGr_G

Cuts at:   1261    1261 
   Size:       4241
 
BamHI G'GATC_C

Cuts at:   1266    1266 
   Size:       4241
 
BanI G'GyrC_C

Cuts at:    113     230     960    1257    1671    1919    1954     113 
   Size:        117     730     297     414     248      35    2400

  Fragments arranged by size: 

                2400     730     414     297     248     117      35
 
BanII G_rGCy'C

Cuts at:    860    1255    2285     860 
   Size:        395    1030    2816

  Fragments arranged by size: 

                2816    1030     395
 
BbeI G_GCGC'C

Cuts at:   1675    1923    1675 
   Size:        248    3993
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    326     360     326 
   Size:         34    4207
 
BciVI GTATCCnnnnn_n'

Cuts at:    387    2139    2900    4241     387 
   Size:       1752     761    1341     387

  Fragments arranged by size: 

                1752    1341     761     387
 
BclI T'GATC_A

Cuts at:   1097    1761    1097 
   Size:        664    3577
 
BglII A'GATC_T

Cuts at:   1756    3793    1756 
   Size:       2037    2204
 
BlpI GC'TnA_GC

Cuts at:    351    1353     351 
   Size:       1002    3239
 
Bme1580I G_kGCm'C

Cuts at:   1866    1959    3015    1866 
   Size:         93    1056    3092

  Fragments arranged by size: 

                3092    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1453    1534    1869    4013    1453 
   Size:         81     335    2144    1681

  Fragments arranged by size: 

                2144    1681     335      81
 
BmtI G_CTAG'C

Cuts at:    820     820 
   Size:       4241
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2608    2608 
   Size:       4241
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    774     774 
   Size:       4241
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    686    1417    2296    2788    3086     686 
   Size:        731     879     492     298    1841

  Fragments arranged by size: 

                1841     879     731     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    332     439    3945     332 
   Size:        107    3506     628

  Fragments arranged by size: 

                3506     628     107
 
BsaAI yAC'GTr

Cuts at:   1594    1594 
   Size:       4241
 
BsaBI GATnn'nnATC

Cuts at:      6    1755    3792       6 
   Size:       1749    2037     455

  Fragments arranged by size: 

                2037    1749     455
 
BsaHI Gr'CG_yC

Cuts at:   1672    1920    2622    4118    1672 
   Size:        248     702    1496    1795

  Fragments arranged by size: 

                1795    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1430    1951    2629    2903    3050    3461    1430 
   Size:        521     678     274     147     411    2210

  Fragments arranged by size: 

                2210     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    179     970    1137     179 
   Size:        791     167    3283

  Fragments arranged by size: 

                3283     791     167
 
BseYI C'CCAG_C

Cuts at:   1666    2424    3001    3497    1666 
   Size:        758     577     496    2410

  Fragments arranged by size: 

                2410     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1783    1829    3037     104 
   Size:       1679      46    1208    1308

  Fragments arranged by size: 

                1679    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    710    1255    2033    2223    3015     710 
   Size:        545     778     190     792    1936

  Fragments arranged by size: 

                1936     792     778     545     190
 
BsmI GAATG_Cn'

Cuts at:    570     676     866     570 
   Size:        106     190    3945

  Fragments arranged by size: 

                3945     190     106
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1203    1203 
   Size:       4241
 
Bsp1286I G_dGCh'C

Cuts at:    710     860    1255    1866    1959    2033    2223    2285    3015 
   Size:        150     395     611      93      74     190      62     730

Cuts at:   3015     710 
   Size:       1936

  Fragments arranged by size: 

                1936     730     611     395     190     150      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3461    3461 
   Size:       4241
 
BspHI T'CATG_A

Cuts at:   3417    3417 
   Size:       4241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1291    1505    1807    2188    1291 
   Size:        214     302     381    3344

  Fragments arranged by size: 

                3344     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     350    2533    2587    4071    4236      50 
   Size:        300    2183      54    1484     165      55

  Fragments arranged by size: 

                2183    1484     300     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2153    2153 
   Size:       4241
 
BsrFI r'CCGG_y

Cuts at:   2239    2420    2629    2239 
   Size:        181     209    3851

  Fragments arranged by size: 

                3851     209     181
 
BssSI C'ACGA_G

Cuts at:   2512    2870    2512 
   Size:        358    3883
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1618    1618 
   Size:       4241
 
BstBI TT'CG_AA

Cuts at:   2602    2602 
   Size:       4241
 
BstXI CCAn_nnnn'nTGG

Cuts at:    438    1045     438 
   Size:        607    3634
 
BstYI r'GATC_y

Cuts at:      1    1266    1733    1756    2091    2337    3349    3435    3447 
   Size:       1265     467      23     335     246    1012      86      12

Cuts at:   3447    3793       1 
   Size:        346     449

  Fragments arranged by size: 

                1265    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    965     965 
   Size:       4241
 
BtgI C'CryG_G

Cuts at:   1075    1075 
   Size:       4241
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2191    2372    2535    2191 
   Size:        181     163    3897

  Fragments arranged by size: 

                3897     181     163
 
BtsI GCAGTG_nn'

Cuts at:    342     800     913    1144    1484     342 
   Size:        458     113     231     340    3099

  Fragments arranged by size: 

                3099     458     340     231     113
 
DraI TTT'AAA

Cuts at:   1240    1240 
   Size:       4241
 
DrdI GACnn_nn'nnGTC

Cuts at:   1947    2805    1947 
   Size:        858    3383
 
EaeI y'GGCC_r

Cuts at:    466    1826    2000    2391    2418    3763     466 
   Size:       1360     174     391      27    1345     944

  Fragments arranged by size: 

                1360    1345     944     391     174      27
 
EagI C'GGCC_G

Cuts at:   1826    1826 
   Size:       4241
 
EarI CTCTTCn'nnn_

Cuts at:   2264    2474    2264 
   Size:        210    4031
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2449    2759    2905    4051    2449 
   Size:        310     146    1146    2639

  Fragments arranged by size: 

                2639    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1253    1253 
   Size:       4241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    130     178     658     764     774    2066    2498    3245    3513 
   Size:         48     480     106      10    1292     432     747     268

Cuts at:   3513     130 
   Size:        858

  Fragments arranged by size: 

                1292     858     747     480     432     268     106      48
                  10
 
EcoO109I rG'GnC_Cy

Cuts at:   1380    1380 
   Size:       4241
 
EcoRI G'AATT_C

Cuts at:   1245    1245 
   Size:       4241
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2260    2260 
   Size:       4241
 
FspI TGC'GCA

Cuts at:   2022    2022 
   Size:       4241
 
HaeII r_GCGC'y

Cuts at:   1675    1923    2945    3924    1675 
   Size:        248    1022     979    1992

  Fragments arranged by size: 

                1992    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    420     452    1173    1205    2083    2113    2115    2145     420 
   Size:         32     721      32     878      30       2      30    2516

  Fragments arranged by size: 

                2516     878     721      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1131    1280    1131 
   Size:        149    4092
 
HindIII A'AGCT_T

Cuts at:    204     204 
   Size:       4241
 
KasI G'GCGC_C

Cuts at:   1671    1919    1671 
   Size:        248    3993
 
KpnI G_GTAC'C

Cuts at:    117    1261     117 
   Size:       1144    3097
 
MluI A'CGCG_T

Cuts at:   3674    3674 
   Size:       4241
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1002    2912    3096    1002 
   Size:       1910     184    2147

  Fragments arranged by size: 

                2147    1910     184
 
MscI TGG'CCA

Cuts at:    468    2002     468 
   Size:       1534    2707
 
MslI CAynn'nnrTG

Cuts at:    871    1155    2230    2357     871 
   Size:        284    1075     127    2755

  Fragments arranged by size: 

                2755    1075     284     127
 
MspA1I CmG'CkG

Cuts at:   1352    1666    2026    3039    3284    1352 
   Size:        314     360    1013     245    2309

  Fragments arranged by size: 

                2309    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2422    2422 
   Size:       4241
 
NarI GG'CG_CC

Cuts at:   1672    1920    1672 
   Size:        248    3993
 
NcoI C'CATG_G

Cuts at:   1075    1075 
   Size:       4241
 
NgoMIV G'CCGG_C

Cuts at:   2420    2420 
   Size:       4241
 
NheI G'CTAG_C

Cuts at:    816     816 
   Size:       4241
 
NspI r_CATG'y

Cuts at:   1294    1794    2701    1294 
   Size:        500     907    2834

  Fragments arranged by size: 

                2834     907     500
 
PciI A'CATG_T

Cuts at:   2697    2697 
   Size:       4241
 
PmeI GTTT'AAAC

Cuts at:   1240    1240 
   Size:       4241
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3380    3412    3380 
   Size:         32    4209
 
PstI C_TGCA'G

Cuts at:   1288    1973    1288 
   Size:        685    3556
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4241
 
PvuII CAG'CTG

Cuts at:   1666    2026    1666 
   Size:        360    3881
 
RsrII CG'GwC_CG

Cuts at:   2436    2436 
   Size:       4241
 
SacI G_AGCT'C

Cuts at:   1255    1255 
   Size:       4241
 
SalI G'TCGA_C

Cuts at:   1278    1278 
   Size:       4241
 
SapI GCTCTTCn'nnn_

Cuts at:   2264    2474    2264 
   Size:        210    4031
 
SbfI CC_TGCA'GG

Cuts at:   1288    1288 
   Size:       4241
 
ScaI AGT'ACT

Cuts at:   3758    3758 
   Size:       4241
 
SfcI C'TryA_G

Cuts at:     33    1284    1969    2962    3153      33 
   Size:       1251     685     993     191    1121

  Fragments arranged by size: 

                1251    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1673    1921    1673 
   Size:        248    3993
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4241
 
SmaI CCC'GGG

Cuts at:   1263    1263 
   Size:       4241
 
SmlI C'TyrA_G

Cuts at:    701    1396    2311    2803    3065     701 
   Size:        695     915     492     262    1877

  Fragments arranged by size: 

                1877     915     695     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4241
 
SphI G_CATG'C

Cuts at:   1294    1794    1294 
   Size:        500    3741
 
SspI AAT'ATT

Cuts at:   1178    1178 
   Size:       4241
 
StyI C'CwwG_G

Cuts at:   1075    1375    1729    1075 
   Size:        300     354    3587

  Fragments arranged by size: 

                3587     354     300
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1824    1957    2416    2625    1824 
   Size:        133     459     209    3440

  Fragments arranged by size: 

                3440     459     209     133
 
TatI w'GTAC_w

Cuts at:   3756    3756 
   Size:       4241
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    109     141    3758    3790     109 
   Size:         32    3617      32     560

  Fragments arranged by size: 

                3617     560      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2038    2038 
   Size:       4241
 
XbaI T'CTAG_A

Cuts at:   1272    1272 
   Size:       4241
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    819    1736     819 
   Size:        917    3324
 
XmaI C'CCGG_G

Cuts at:   1261    1261 
   Size:       4241
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NspI     PciI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI   Bpu10I
    BsaXI     BsgI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI
     NdeI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI
     XhoI     XmnI     ZraI
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