Restriction map for pF1KE0008
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        Sse8387I    StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI         1329  GTCTAC               GT^[AC][GT]AC
AflIII       1305  ACATGT               A^C[GA][CT]GT
AhdI          791  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         740  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI         1675  ATTAAT               AT^TAAT
AsnI         1675  ATTAAT               AT^TAAT
AspEI         791  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         408  GTGCTC               G[AT]GC[AT]^C
AvaII        1474  GGACC                G^G[AT]CC
BalI         1904  TGGCCA               TGG^CCA
BcnI         1613  CCCGG                CC^[GC]GG
BpmI          478  CTGGAG               CTGGAG(16/14)
BsaI         1381  GAGACC               GGTCTC(1/5)
BsiHKAI       408  GTGCTC               G[AT]GC[AT]^C
BsmFI        1824  GGGAC                GGGAC(10/14)
Bsp1407I     1308  TGTACA               T^GTACA
BspLU11I     1305  ACATGT               A^CATGT
BspMI        1543  GCAGGT               ACCTGC(4/8)
BsrGI        1308  TGTACA               T^GTACA
BstYI        1991  GGATCT               [GA]^GATC[CT]
CfoI         1047  GCGC                 GCG^C
DraI          340  TTTAAA               TTT^AAA
EaeI         1904  TGGCCA               [CT]^GGCC[GA]
Eam1105I      791  GACTTTCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1195  CTCTTC               CTCTTC(1/4)
EcoT22I        85  ATGCAT               ATGCA^T
FauI         1743  CCCGC                CCCGC(4/6)
HapII        1614  CCGG                 C^CGG
HgaI         1826  GACGC                GACGC(5/10)
HhaI         1047  GCGC                 GCG^C
HinP1I       1047  GCGC                 G^CGC
HindIII      1445  AAGCTT               A^AGCTT
HpaII        1614  CCGG                 C^CGG
Ksp632I      1195  CTCTTC               CTCTTC(1/4)
MflI         1991  GGATCT               [GA]^GATC[CT]
MluNI        1904  TGGCCA               TGG^CCA
MscI         1904  TGGCCA               TGG^CCA
MspI         1614  CCGG                 C^CGG
NciI         1613  CCCGG                CC^[GC]GG
NdeI         1372  CATATG               CA^TATG
NsiI           85  ATGCAT               ATGCA^T
PshBI        1675  ATTAAT               AT^TAAT
PstI         1266  CTGCAG               CTGCA^G
SapI         1194  GCTCTTC              GCTCTTC(1/4)
SfcI         1266  CTGCAG               C^T[GA][CT]AG
SmlI         1146  CTTGAG               C^T[CT][GA]AG
SphI         1337  GCATGC               GCATG^C
SspBI        1308  TGTACA               T^GTACA
SspI          371  AATATT               AAT^ATT
TaqI         1454  TCGA                 T^CGA
TthHB8I      1454  TCGA                 T^CGA
XhoII        1991  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI          706  GATCC                GGATC(4/5)
             1991  GGATC               
Asp700I       588  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              788  GAAGACTTTC          
BanII        1192  GGGCTC               G[GA]GC[CT]^C
             1535  GGGCTC              
BbsI          788  GAAGAC               GAAGAC(2/6)
             1088  GTCTTC              
BbvI          265  GCAGC                GCAGC(8/12)
             1125  GCAGC               
BpuAI         788  GAAGAC               GAAGAC(2/6)
             1088  GTCTTC              
BsaAI         157  TACGTA               [CT]AC^GT[GA]
             1488  TACGTA              
BsrDI         498  CATTGC               GCAATG(2/0)
               46  GCAATG              
BstNI         886  CCTGG                CC^[AT]GG
             1121  CCTGG               
Cfr13I        449  GGCCC                G^G[ACGT]CC
             1474  GGACC               
EcoRII        886  CCTGG                ^CC[AT]GG
             1121  CCTGG               
Fnu4HI        265  GCAGC                GC^[ACGT]GC
             1125  GCAGC               
ItaI          265  GCAGC                GC^[ACGT]GC
             1125  GCAGC               
MaeII         158  ACGT                 A^CGT
             1489  ACGT                
MslI          507  CACAACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1513  CACACCCATG          
MvaI          886  CCTGG                CC^[AT]GG
             1121  CCTGG               
MwoI         1049  GCCATTTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1284  GCCAACACTGC         
NcoI         1181  CCATGG               C^CATGG
             1518  CCATGG              
NlaIV        1474  GGACCC               GG[ACGT]^[ACGT]CC
             1600  GGTTCC              
Sau96I        449  GGCCC                G^G[ACGT]CC
             1474  GGACC               
SfaNI        1044  GATGC                GCATC(5/9)
             1033  GCATC               
SnaBI         157  TACGTA               TAC^GTA
             1488  TACGTA              
TseI          265  GCAGC                G^C[AT]GC
             1125  GCAGC               
XmnI          588  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              788  GAAGACTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsmI        Bsp1286I    BsrI        BssKI       
BstF5I      Cac8I       DdeI        DpnII       DsaI        
EcoT14I     FokI        HaeIII      HinfI       HphI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        NdeII       NlaIII      NspI        PflMI       
RsaI        Sau3AI      ScrFI       StyI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Van91I      
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