Restriction map for pF1KE0115
 (Circular) (Six-base) MAPSORT of: pF1KE0115.seq  Check: 4396  from: 1  to: 3797



pF1KE0115  3797 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:    835     835 
   Size:       3797
 
Acc65I G'GTAC_C

Cuts at:    813     813 
   Size:       3797
 
AclI AA'CG_TT

Cuts at:   3637    3637 
   Size:       3797
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1622    2054    2801    3069    1622 
   Size:        432     747     268    2350

  Fragments arranged by size: 

                2350     747     432     268
 
AflII C'TTAA_G

Cuts at:    549     690     549 
   Size:        141    3656
 
AflIII A'CryG_T

Cuts at:    616     674    1149    2253    3230     616 
   Size:         58     475    1104     977    1183

  Fragments arranged by size: 

                1183    1104     977     475      58
 
AgeI A'CCGG_T

Cuts at:   2185    2185 
   Size:       3797
 
AlwNI CAG_nnn'CTG

Cuts at:   1102    2669    1102 
   Size:       1567    2230
 
ApaLI G'TGCA_C

Cuts at:   2567    2567 
   Size:       3797
 
ApoI r'AATT_y

Cuts at:    234     630     801    3150    3406     234 
   Size:        396     171    2349     256     625

  Fragments arranged by size: 

                2349     625     396     256     171
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3797
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3797
 
AvaI C'yCGr_G

Cuts at:    817     817 
   Size:       3797
 
BamHI G'GATC_C

Cuts at:    189     822     189 
   Size:        633    3164
 
BanI G'GyrC_C

Cuts at:    813    1227    1475    1510     813 
   Size:        414     248      35    3100

  Fragments arranged by size: 

                3100     414     248      35
 
BanII G_rGCy'C

Cuts at:    811    1841     811 
   Size:       1030    2767
 
BbeI G_GCGC'C

Cuts at:   1231    1479    1231 
   Size:        248    3549
 
BbsI GAAGACnn'nnnn_

Cuts at:    691     691 
   Size:       3797
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3763
 
BciVI GTATCCnnnnn_n'

Cuts at:   1695    2456    3797    1695 
   Size:        761    1341    1695

  Fragments arranged by size: 

                1695    1341     761
 
BclI T'GATC_A

Cuts at:   1317    1317 
   Size:       3797
 
BglII A'GATC_T

Cuts at:   1312    3349    1312 
   Size:       2037    1760
 
BlpI GC'TnA_GC

Cuts at:    909     909 
   Size:       3797
 
Bme1580I G_kGCm'C

Cuts at:   1422    1515    2571    1422 
   Size:         93    1056    2648

  Fragments arranged by size: 

                2648    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1009    1090    1425    3569    1009 
   Size:         81     335    2144    1237

  Fragments arranged by size: 

                2144    1237     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2164    2164 
   Size:       3797
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    404     404 
   Size:       3797
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    364     973    1852    2344    2642     364 
   Size:        609     879     492     298    1519

  Fragments arranged by size: 

                1519     879     609     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3501    3501 
   Size:       3797
 
BsaAI yAC'GTr

Cuts at:    617    1150     617 
   Size:        533    3264
 
BsaBI GATnn'nnATC

Cuts at:      6    1311    3348       6 
   Size:       1305    2037     455

  Fragments arranged by size: 

                2037    1305     455
 
BsaHI Gr'CG_yC

Cuts at:   1228    1476    2178    3674    1228 
   Size:        248     702    1496    1351

  Fragments arranged by size: 

                1496    1351     702     248
 
BsaWI w'CCGG_w

Cuts at:    986    1507    2185    2459    2606    3017     986 
   Size:        521     678     274     147     411    1766

  Fragments arranged by size: 

                1766     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1222    1980    2557    3053    1222 
   Size:        758     577     496    1966

  Fragments arranged by size: 

                1966     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1339    1385    2593     104 
   Size:       1235      46    1208    1308

  Fragments arranged by size: 

                1308    1235    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    811    1589    1779    2571     811 
   Size:        778     190     792    2037

  Fragments arranged by size: 

                2037     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    811    1422    1515    1589    1779    1841    2571     811 
   Size:        611      93      74     190      62     730    2037

  Fragments arranged by size: 

                2037     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3017    3017 
   Size:       3797
 
BspHI T'CATG_A

Cuts at:   2973    2973 
   Size:       3797
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    847    1061    1363    1744     847 
   Size:        214     302     381    2900

  Fragments arranged by size: 

                2900     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2089    2143    3627    3792      50 
   Size:       2039      54    1484     165      55

  Fragments arranged by size: 

                2039    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1709    1709 
   Size:       3797
 
BsrFI r'CCGG_y

Cuts at:   1795    1976    2185    1795 
   Size:        181     209    3407

  Fragments arranged by size: 

                3407     209     181
 
BssSI C'ACGA_G

Cuts at:   2068    2426    2068 
   Size:        358    3439
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1174    1174 
   Size:       3797
 
BstBI TT'CG_AA

Cuts at:   2158    2158 
   Size:       3797
 
BstXI CCAn_nnnn'nTGG

Cuts at:    621     621 
   Size:       3797
 
BstYI r'GATC_y

Cuts at:      1     189     719     822    1289    1312    1647    1893    2905 
   Size:        188     530     103     467      23     335     246    1012

Cuts at:   2905    2991    3003    3349       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     530     467     449     346     335     246     188
                 103      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1747    1928    2091    1747 
   Size:        181     163    3453

  Fragments arranged by size: 

                3453     181     163
 
BtsI GCAGTG_nn'

Cuts at:    748    1040     748 
   Size:        292    3505
 
DraI TTT'AAA

Cuts at:    440     796     440 
   Size:        356    3441
 
DrdI GACnn_nn'nnGTC

Cuts at:   1503    2361    1503 
   Size:        858    2939
 
EaeI y'GGCC_r

Cuts at:   1382    1556    1947    1974    3319    1382 
   Size:        174     391      27    1345    1860

  Fragments arranged by size: 

                1860    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1382    1382 
   Size:       3797
 
EarI CTCTTCn'nnn_

Cuts at:   1820    2030    1820 
   Size:        210    3587
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2005    2315    2461    3607    2005 
   Size:        310     146    1146    2195

  Fragments arranged by size: 

                2195    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    809     809 
   Size:       3797
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    404    1622    2054    2801    3069     404 
   Size:       1218     432     747     268    1132

  Fragments arranged by size: 

                1218    1132     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    577     577 
   Size:       3797
 
EcoO109I rG'GnC_Cy

Cuts at:    936     936 
   Size:       3797
 
EcoRI G'AATT_C

Cuts at:    801     801 
   Size:       3797
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    487     801    1816     487 
   Size:        314    1015    2468

  Fragments arranged by size: 

                2468    1015     314
 
FspI TGC'GCA

Cuts at:   1578    1578 
   Size:       3797
 
HaeII r_GCGC'y

Cuts at:   1231    1479    2501    3480    1231 
   Size:        248    1022     979    1548

  Fragments arranged by size: 

                1548    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1639    1669    1671    1701    1639 
   Size:         30       2      30    3735

  Fragments arranged by size: 

                3735      30      30       2
 
HincII GTy'rAC

Cuts at:    836     836 
   Size:       3797
 
HindIII A'AGCT_T

Cuts at:    546     546 
   Size:       3797
 
KasI G'GCGC_C

Cuts at:   1227    1475    1227 
   Size:        248    3549
 
KpnI G_GTAC'C

Cuts at:    817     817 
   Size:       3797
 
MfeI C'AATT_G

Cuts at:    117     117 
   Size:       3797
 
MluI A'CGCG_T

Cuts at:   3230    3230 
   Size:       3797
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    361    2468    2652     361 
   Size:       2107     184    1506

  Fragments arranged by size: 

                2107    1506     184
 
MscI TGG'CCA

Cuts at:   1558    1558 
   Size:       3797
 
MslI CAynn'nnrTG

Cuts at:    482    1786    1913     482 
   Size:       1304     127    2366

  Fragments arranged by size: 

                2366    1304     127
 
MspA1I CmG'CkG

Cuts at:    424     908    1222    1582    2595    2840     424 
   Size:        484     314     360    1013     245    1381

  Fragments arranged by size: 

                1381    1013     484     360     314     245
 
NaeI GCC'GGC

Cuts at:   1978    1978 
   Size:       3797
 
NarI GG'CG_CC

Cuts at:   1228    1476    1228 
   Size:        248    3549
 
NgoMIV G'CCGG_C

Cuts at:   1976    1976 
   Size:       3797
 
NspI r_CATG'y

Cuts at:    678     850    1350    2257     678 
   Size:        172     500     907    2218

  Fragments arranged by size: 

                2218     907     500     172
 
PciI A'CATG_T

Cuts at:    674    2253     674 
   Size:       1579    2218
 
PmeI GTTT'AAAC

Cuts at:    796     796 
   Size:       3797
 
PmlI CAC'GTG

Cuts at:    617     617 
   Size:       3797
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2936    2968    2936 
   Size:         32    3765
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    662     694     662 
   Size:         32    3765
 
PstI C_TGCA'G

Cuts at:    844    1529     844 
   Size:        685    3112
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3797
 
PvuII CAG'CTG

Cuts at:    424    1222    1582     424 
   Size:        798     360    2639

  Fragments arranged by size: 

                2639     798     360
 
RsrII CG'GwC_CG

Cuts at:   1992    1992 
   Size:       3797
 
SacI G_AGCT'C

Cuts at:    811     811 
   Size:       3797
 
SalI G'TCGA_C

Cuts at:    834     834 
   Size:       3797
 
SapI GCTCTTCn'nnn_

Cuts at:   1820    2030    1820 
   Size:        210    3587
 
SbfI CC_TGCA'GG

Cuts at:    844     844 
   Size:       3797
 
ScaI AGT'ACT

Cuts at:    467    3314     467 
   Size:       2847     950
 
SfcI C'TryA_G

Cuts at:     33     840    1525    2518    2709      33 
   Size:        807     685     993     191    1121

  Fragments arranged by size: 

                1121     993     807     685     191
 
SfoI GGC'GCC

Cuts at:   1229    1477    1229 
   Size:        248    3549
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3797
 
SmaI CCC'GGG

Cuts at:    819     819 
   Size:       3797
 
SmlI C'TyrA_G

Cuts at:    379     549     690     952    1867    2359    2621     379 
   Size:        170     141     262     915     492     262    1555

  Fragments arranged by size: 

                1555     915     492     262     262     170     141
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3797
 
SphI G_CATG'C

Cuts at:    850    1350     850 
   Size:        500    3297
 
StyI C'CwwG_G

Cuts at:    704     931    1285     704 
   Size:        227     354    3216

  Fragments arranged by size: 

                3216     354     227
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1380    1513    1972    2181    1380 
   Size:        133     459     209    2996

  Fragments arranged by size: 

                2996     459     209     133
 
TatI w'GTAC_w

Cuts at:    465    3312     465 
   Size:       2847     950
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3314    3346    3314 
   Size:         32    3765
 
Tth111I GACn'n_nGTC

Cuts at:   1594    1594 
   Size:       3797
 
XbaI T'CTAG_A

Cuts at:    828     828 
   Size:       3797
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1292    1292 
   Size:       3797
 
XmaI C'CCGG_G

Cuts at:    817     817 
   Size:       3797
 
XmnI GAAnn'nnTTC

Cuts at:    212     212 
   Size:       3797
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
     PmeI     PmlI     PpiI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoRV     FseI    FspAI     HpaI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI    PpuMI    PshAI
     PsiI   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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