Restriction map for pF1KB7319
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BsaBI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrGI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KpnI        KspI        MaeI        MamI        MluI        
MroI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AatII         747  GACGTC               GACGT^C
Asp700I      1377  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          919  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          996  CCCGGG               C^[CT]CG[GA]G
BbeI          883  GGCGCC               GGCGC^C
BbsI         1669  GTCTTC               GAAGAC(2/6)
BglII        1268  AGATCT               A^GATCT
BlpI           55  GCTGAGC              GC^T[ACGT]AGC
BpmI         1761  CTGGAG               CTGGAG(16/14)
Bpu1102I       55  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1669  GTCTTC               GAAGAC(2/6)
BsaAI        1861  TACGTG               [CT]AC^GT[GA]
BsaWI        1367  ACCGGA               [AT]^CCGG[AT]
BsmI         1600  GAATGC               GAATGC(1/-1)
BsoBI         996  CCCGGG               C^[CT]CG[GA]G
BspHI         440  TCATGA               T^CATGA
BsrDI        1359  GCAATG               GCAATG(2/0)
BssHII        805  GCGCGC               G^CGCGC
BssSI         349  CACGAG               CACGAG(-5/-1)
BstYI        1268  AGATCT               [GA]^GATC[CT]
CelII          55  GCTGAGC              GC^T[ACGT]AGC
DrdI         2374  GACCTCTTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         210  CCTCATGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       1279  GTCAAC               GT[CT]^[GA]AC
HindII       1279  GTCAAC               GT[CT]^[GA]AC
KasI          883  GGCGCC               G^GCGCC
MfeI         2147  CAATTG               C^AATTG
MflI         1268  AGATCT               [GA]^GATC[CT]
MseI          152  TTAA                 T^TAA
MunI         2147  CAATTG               C^AATTG
NaeI           60  GCCGGC               GCC^GGC
NarI          883  GGCGCC               GG^CGCC
NcoI          578  CCATGG               C^CATGG
NgoMIV         60  GCCGGC               G^CCGGC
NspI         2087  GCATGC               [GA]CATG^[CT]
PflFI         919  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          47  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          440  TCATGA               T^CATGA
SbfI          159  CCTGCAGG             CCTGCA^GG
SfiI         1707  GGCCCTGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          883  GGCGCC               GGC^GCC
SmaI          996  CCCGGG               CCC^GGG
SmlI         1261  CTTGAG               C^T[CT][GA]AG
SphI         2087  GCATGC               GCATG^C
Sse8387I      159  CCTGCAGG             CCTGCA^GG
StuI         1607  AGGCCT               AGG^CCT
TfiI         1377  GAATC                G^A[AT]TC
Tru9I         152  TTAA                 T^TAA
Tth111I       919  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1268  AGATCT               [GA]^GATC[CT]
XmaCI         996  CCCGGG               C^CCGGG
XmaI          996  CCCGGG               C^CCGGG
XmnI         1377  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1153  GTCTAC               GT^[AC][GT]AC
             1243  GTCTAC              
AcyI          747  GACGTC               G[GA]^CG[CT]C
              883  GGCGCC              
AlwNI         763  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2007  CAGCTACTG           
BsaHI         747  GACGTC               G[GA]^CG[CT]C
              883  GGCGCC              
BsaI         1642  GAGACC               GGTCTC(1/5)
             1878  GGTCTC              
BsrFI          60  GCCGGC               [GA]^CCGG[CT]
              327  GCCGGT              
BstEII        771  GGTGACC              G^GT[ACGT]ACC
             2361  GGTCACC             
BstPI         771  GGTGACC              G^GT[ACGT]ACC
             2361  GGTCACC             
Cfr10I         60  GCCGGC               [GA]^CCGG[CT]
              327  GCCGGT              
DsaI          578  CCATGG               C^C[GA][CT]GG
             2096  CCACGG              
EarI          112  CTCTTC               CTCTTC(1/4)
             2446  CTCTTC              
EcoO65I       771  GGTGACC              G^GT[ACGT]ACC
             2361  GGTCACC             
EcoT14I       578  CCATGG               C^C[AT][AT]GG
             1874  CCAAGG              
Hin1I         747  GACGTC               G[GA]^CG[CT]C
              883  GGCGCC              
Ksp632I       112  CTCTTC               CTCTTC(1/4)
             2446  CTCTTC              
PflMI         762  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1027  CCACAGGCTGG         
PleI          433  GAGTC                GAGTC(4/5)
              904  GAGTC               
StyI          578  CCATGG               C^C[AT][AT]GG
             1874  CCAAGG              
Tsp509I      2082  AATT                 ^AATT
             2148  AATT                
Van91I        762  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1027  CCACAGGCTGG         
XcmI          870  CCAGAACTTCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1694  CCACCATCGCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaII       BalI        BanI        
BanII       BbvCI       BbvI        BcnI        BglI        
BmyI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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