Restriction map for pF1KB7319
 (Circular) (Six-base) MAPSORT of: pF1KB7319.seq  Check: 8793  from: 1  to: 5630



pF1KB7319  5630 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    261    1342    2002     261 
   Size:       1081     660    3889

  Fragments arranged by size: 

                3889    1081     660
 
AatII G_ACGT'C

Cuts at:    859     859 
   Size:       5630
 
AccI GT'mk_AC

Cuts at:   1262    1352    2668    1262 
   Size:         90    1316    4224

  Fragments arranged by size: 

                4224    1316      90
 
Acc65I G'GTAC_C

Cuts at:   2646    2646 
   Size:       5630
 
AclI AA'CG_TT

Cuts at:   5470    5470 
   Size:       5630
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1095    1552    1715    3455    3887    4634    4902    1095 
   Size:        457     163    1740     432     747     268    1823

  Fragments arranged by size: 

                1823    1740     747     457     432     268     163
 
AflIII A'CryG_T

Cuts at:   2982    4086    5063    2982 
   Size:       1104     977    3549

  Fragments arranged by size: 

                3549    1104     977
 
AgeI A'CCGG_T

Cuts at:   4018    4018 
   Size:       5630
 
AleI CACnn'nnGTG

Cuts at:   2476    2476 
   Size:       5630
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    388    2305    2377     388 
   Size:       1917      72    3641

  Fragments arranged by size: 

                3641    1917      72
 
AlwNI CAG_nnn'CTG

Cuts at:    876    2120    2935    4502     876 
   Size:       1244     815    1567    2004

  Fragments arranged by size: 

                2004    1567    1244     815
 
ApaI G_GGCC'C

Cuts at:    116    1135    1912    2141     116 
   Size:       1019     777     229    3605

  Fragments arranged by size: 

                3605    1019     777     229
 
ApaLI G'TGCA_C

Cuts at:   4400    4400 
   Size:       5630
 
ApoI r'AATT_y

Cuts at:   2634    4983    5239    2634 
   Size:       2349     256    3025

  Fragments arranged by size: 

                3025    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5630
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5630
 
AvaI C'yCGr_G

Cuts at:   1104    2650    1104 
   Size:       1546    4084
 
BamHI G'GATC_C

Cuts at:   2655    2655 
   Size:       5630
 
BanI G'GyrC_C

Cuts at:    273     991    1066    1601    1675    1797    2646    3060    3308 
   Size:        718      75     535      74     122     849     414     248

Cuts at:   3308    3343     273 
   Size:         35    2560

  Fragments arranged by size: 

                2560     849     718     535     414     248     122      75
                  74      35
 
BanII G_rGCy'C

Cuts at:    116    1135    1912    2141    2644    3674     116 
   Size:       1019     777     229     503    1030    2072

  Fragments arranged by size: 

                2072    1030    1019     777     503     229
 
BbeI G_GCGC'C

Cuts at:    995    3064    3312     995 
   Size:       2069     248    3313

  Fragments arranged by size: 

                3313    2069     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1770    1770 
   Size:       5630
 
BbvCI CC'TCA_GC

Cuts at:   1156    1840    2398    1156 
   Size:        684     558    4388

  Fragments arranged by size: 

                4388     684     558
 
BciVI GTATCCnnnnn_n'

Cuts at:   3528    4289    5630    3528 
   Size:        761    1341    3528

  Fragments arranged by size: 

                3528    1341     761
 
BclI T'GATC_A

Cuts at:   3150    3150 
   Size:       5630
 
BglI GCCn_nnn'nGGC

Cuts at:   1064    1316    1673    1822    1064 
   Size:        252     357     149    4872

  Fragments arranged by size: 

                4872     357     252     149
 
BglII A'GATC_T

Cuts at:   1376    3145    5182    1376 
   Size:       1769    2037    1824

  Fragments arranged by size: 

                2037    1824    1769
 
BlpI GC'TnA_GC

Cuts at:    164    2742     164 
   Size:       2578    3052
 
Bme1580I G_kGCm'C

Cuts at:    116     278    1069    1100    1135    1912    2141    3255    3348 
   Size:        162     791      31      35     777     229    1114      93

Cuts at:   3348    4404     116 
   Size:       1056    1342

  Fragments arranged by size: 

                1342    1114    1056     791     777     229     162      93
                  35      31
 
BmgBI CAC'GTC

Cuts at:   1437    1641    1437 
   Size:        204    5426
 
BmrI ACTGGGnnnn_n'

Cuts at:   2130    2842    2923    3258    5402    2130 
   Size:        712      81     335    2144    2358

  Fragments arranged by size: 

                2358    2144     712     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3997    3997 
   Size:       5630
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1890    1890 
   Size:       5630
 
Bpu10I CC'TnA_GC

Cuts at:   1156    1840    2398    1156 
   Size:        684     558    4388

  Fragments arranged by size: 

                4388     684     558
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1390    2806    3685    4177    4475    1390 
   Size:       1416     879     492     298    2545

  Fragments arranged by size: 

                2545    1416     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1744    1992    5334    1744 
   Size:        248    3342    2040

  Fragments arranged by size: 

                3342    2040     248
 
BsaAI yAC'GTr

Cuts at:   1971    2983    1971 
   Size:       1012    4618
 
BsaBI GATnn'nnATC

Cuts at:      6    3144    5181       6 
   Size:       3138    2037     455

  Fragments arranged by size: 

                3138    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    856     992    3061    3309    4011    5507     856 
   Size:        136    2069     248     702    1496     979

  Fragments arranged by size: 

                2069    1496     979     702     248     136
 
BsaWI w'CCGG_w

Cuts at:   1475    2819    3340    4018    4292    4439    4850    1475 
   Size:       1344     521     678     274     147     411    2255

  Fragments arranged by size: 

                2255    1344     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    553     583     553 
   Size:         30    5600
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    123     361     877    1303    1387    2631     123 
   Size:        238     516     426      84    1244    3122

  Fragments arranged by size: 

                3122    1244     516     426     238      84
 
BseYI C'CCAG_C

Cuts at:    387     661    1141    1903    3055    3813    4390    4886     387 
   Size:        274     480     762    1152     758     577     496    1131

  Fragments arranged by size: 

                1152    1131     762     758     577     496     480     274
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    688     712    1021    1288    1320    1429    2421     688 
   Size:         24     309     267      32     109     992    3897

  Fragments arranged by size: 

                3897     992     309     267     109      32      24
 
BsiEI CG_ry'CG

Cuts at:    104    3172    3218    4426     104 
   Size:       3068      46    1208    1308

  Fragments arranged by size: 

                3068    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    443     910    2501    2644    3422    3612    4404     443 
   Size:        467    1591     143     778     190     792    1669

  Fragments arranged by size: 

                1669    1591     792     778     467     190     143
 
BsmI GAATG_Cn'

Cuts at:   1714    1714 
   Size:       5630
 
BsmBI CGTCTCn'nnnn_

Cuts at:    814    2151    2433     814 
   Size:       1337     282    4011

  Fragments arranged by size: 

                4011    1337     282
 
Bsp1286I G_dGCh'C

Cuts at:    116     278     443     910    1069    1100    1135    1912    2141 
   Size:        162     165     467     159      31      35     777     229

Cuts at:   2141    2501    2644    3255    3348    3422    3612    3674    4404 
   Size:        360     143     611      93      74     190      62     730

Cuts at:   4404     116 
   Size:       1342

  Fragments arranged by size: 

                1342     777     730     611     467     360     229     190
                 165     162     159     143      93      74      62      35
                  31
 
BspEI T'CCGG_A

Cuts at:   4850    4850 
   Size:       5630
 
BspHI T'CATG_A

Cuts at:    548    4806     548 
   Size:       4258    1372
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    200     261    1189    1342    2002    2321    2680    2894    3196 
   Size:         61     928     153     660     319     359     214     302

Cuts at:   3196    3577     200 
   Size:        381    2253

  Fragments arranged by size: 

                2253     928     660     381     359     319     302     214
                 153      61
 
BsrBI CCG'CTC

Cuts at:     50    3922    3976    5460    5625      50 
   Size:       3872      54    1484     165      55

  Fragments arranged by size: 

                3872    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1474    3542    1474 
   Size:       2068    3562
 
BsrFI r'CCGG_y

Cuts at:    168     435    3628    3809    4018     168 
   Size:        267    3193     181     209    1780

  Fragments arranged by size: 

                3193    1780     267     209     181
 
BssHII G'CGCG_C

Cuts at:    913     913 
   Size:       5630
 
BssSI C'ACGA_G

Cuts at:    457    3901    4259     457 
   Size:       3444     358    1828

  Fragments arranged by size: 

                3444    1828     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    330     699    1664    3007     330 
   Size:        369     965    1343    2953

  Fragments arranged by size: 

                2953    1343     965     369
 
BstBI TT'CG_AA

Cuts at:   3991    3991 
   Size:       5630
 
BstEII G'GTnAC_C

Cuts at:    879    2469     879 
   Size:       1590    4040
 
BstXI CCAn_nnnn'nTGG

Cuts at:    775    1347    1812    2520     775 
   Size:        572     465     708    3885

  Fragments arranged by size: 

                3885     708     572     465
 
BstYI r'GATC_y

Cuts at:      1    1376    2655    3122    3145    3480    3726    4738    4824 
   Size:       1375    1279     467      23     335     246    1012      86

Cuts at:   4824    4836    5182       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1375    1279    1012     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     686    2204     107 
   Size:        579    1518    3533

  Fragments arranged by size: 

                3533    1518     579
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    626     792    1791    3580    3761    3924     626 
   Size:        166     999    1789     181     163    2332

  Fragments arranged by size: 

                2332    1789     999     181     166     163
 
BtsI GCAGTG_nn'

Cuts at:    832    1933    2873     832 
   Size:       1101     940    3589

  Fragments arranged by size: 

                3589    1101     940
 
DraI TTT'AAA

Cuts at:   2629    2629 
   Size:       5630
 
DrdI GACnn_nn'nnGTC

Cuts at:   2488    3336    4194    2488 
   Size:        848     858    3924

  Fragments arranged by size: 

                3924     858     848
 
EaeI y'GGCC_r

Cuts at:    557     753     765    1823    1850    2090    2201    2510    3215 
   Size:        196      12    1058      27     240     111     309     705

Cuts at:   3215    3389    3780    3807    5152     557 
   Size:        174     391      27    1345    1035

  Fragments arranged by size: 

                1345    1058    1035     705     391     309     240     196
                 174     111      27      27      12
 
EagI C'GGCC_G

Cuts at:   3215    3215 
   Size:       5630
 
EarI CTCTTCn'nnn_

Cuts at:    226    2560    3653    3863     226 
   Size:       2334    1093     210    1993

  Fragments arranged by size: 

                2334    1993    1093     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3838    4148    4294    5440    3838 
   Size:        310     146    1146    4028

  Fragments arranged by size: 

                4028    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2642    2642 
   Size:       5630
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1095    1552    1715    1890    3455    3887    4634    4902    1095 
   Size:        457     163     175    1565     432     747     268    1823

  Fragments arranged by size: 

                1823    1565     747     457     432     268     175     163
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    322     322 
   Size:       5630
 
EcoO109I rG'GnC_Cy

Cuts at:    112     156    1131    1908    1909    2010    2137    2138    2769 
   Size:         44     975     777       1     101     127       1     631

Cuts at:   2769     112 
   Size:       2973

  Fragments arranged by size: 

                2973     975     777     631     127     101      44       1
                   1
 
EcoRI G'AATT_C

Cuts at:   2634    2634 
   Size:       5630
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    651    1407    3649     651 
   Size:        756    2242    2632

  Fragments arranged by size: 

                2632    2242     756
 
FspI TGC'GCA

Cuts at:   3411    3411 
   Size:       5630
 
HaeII r_GCGC'y

Cuts at:    605     668     870     876     995    3064    3312    4334    5313 
   Size:         63     202       6     119    2069     248    1022     979

Cuts at:   5313     605 
   Size:        922

  Fragments arranged by size: 

                2069    1022     979     922     248     202     119      63
                   6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1470    1502    3472    3502    3504    3534    1470 
   Size:         32    1970      30       2      30    3566

  Fragments arranged by size: 

                3566    1970      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1389    2669    1389 
   Size:       1280    4350
 
KasI G'GCGC_C

Cuts at:    991    3060    3308     991 
   Size:       2069     248    3313

  Fragments arranged by size: 

                3313    2069     248
 
KpnI G_GTAC'C

Cuts at:   2650    2650 
   Size:       5630
 
MfeI C'AATT_G

Cuts at:   2255    2255 
   Size:       5630
 
MluI A'CGCG_T

Cuts at:   5063    5063 
   Size:       5630
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    446    4301    4485     446 
   Size:       3855     184    1591

  Fragments arranged by size: 

                3855    1591     184
 
MscI TGG'CCA

Cuts at:    559     767    1852    2092    2203    2512    3391     559 
   Size:        208    1085     240     111     309     879    2798

  Fragments arranged by size: 

                2798    1085     879     309     240     208     111
 
MslI CAynn'nnrTG

Cuts at:    553    1810    1858    2443    2476    3619    3746     553 
   Size:       1257      48     585      33    1143     127    2437

  Fragments arranged by size: 

                2437    1257    1143     585     127      48      33
 
MspA1I CmG'CkG

Cuts at:    696     713     771    1365    1530    1773    2459    2741    3055 
   Size:         17      58     594     165     243     686     282     314

Cuts at:   3055    3415    4428    4673     696 
   Size:        360    1013     245    1653

  Fragments arranged by size: 

                1653    1013     686     594     360     314     282     245
                 243     165      58      17
 
NaeI GCC'GGC

Cuts at:    170    3811     170 
   Size:       3641    1989
 
NarI GG'CG_CC

Cuts at:    992    3061    3309     992 
   Size:       2069     248    3313

  Fragments arranged by size: 

                3313    2069     248
 
NcoI C'CATG_G

Cuts at:    107     686     107 
   Size:        579    5051
 
NgoMIV G'CCGG_C

Cuts at:    168    3809     168 
   Size:       3641    1989
 
NspI r_CATG'y

Cuts at:   2199    2683    3183    4090    2199 
   Size:        484     500     907    3739

  Fragments arranged by size: 

                3739     907     500     484
 
PasI CC'CwG_GG

Cuts at:    116    1830     116 
   Size:       1714    3916
 
PciI A'CATG_T

Cuts at:   4086    4086 
   Size:       5630
 
PflMI CCAn_nnn'nTGG

Cuts at:    876    1141     876 
   Size:        265    5365
 
PfoI T'CCnGG_A

Cuts at:    815    1400    1781     815 
   Size:        585     381    4664

  Fragments arranged by size: 

                4664     585     381
 
PmeI GTTT'AAAC

Cuts at:   2629    2629 
   Size:       5630
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2371    2403    4769    4801    2371 
   Size:         32    2366      32    3200

  Fragments arranged by size: 

                3200    2366      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    156     156 
   Size:       5630
 
PspOMI G'GGCC_C

Cuts at:    112    1131    1908    2137     112 
   Size:       1019     777     229    3605

  Fragments arranged by size: 

                3605    1019     777     229
 
PstI C_TGCA'G

Cuts at:    272     839     902    1523    2677    3362     272 
   Size:        567      63     621    1154     685    2540

  Fragments arranged by size: 

                2540    1154     685     621     567      63
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5630
 
PvuII CAG'CTG

Cuts at:    696     771    1365    1530    1773    3055    3415     696 
   Size:         75     594     165     243    1282     360    2911

  Fragments arranged by size: 

                2911    1282     594     360     243     165      75
 
RsrII CG'GwC_CG

Cuts at:   3825    3825 
   Size:       5630
 
SacI G_AGCT'C

Cuts at:   2644    2644 
   Size:       5630
 
SalI G'TCGA_C

Cuts at:   2667    2667 
   Size:       5630
 
SapI GCTCTTCn'nnn_

Cuts at:   3653    3863    3653 
   Size:        210    5420
 
SbfI CC_TGCA'GG

Cuts at:    272    2677     272 
   Size:       2405    3225
 
ScaI AGT'ACT

Cuts at:   5147    5147 
   Size:       5630
 
SfcI C'TryA_G

Cuts at:     33     268     489     597     835     898    1263    1519    2112 
   Size:        235     221     108     238      63     365     256     593

Cuts at:   2112    2454    2673    3358    4351    4542      33 
   Size:        342     219     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     593     365     342     256     238
                 235     221     219     191     108      63
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1822    1822 
   Size:       5630
 
SfoI GGC'GCC

Cuts at:    993    3062    3310     993 
   Size:       2069     248    3313

  Fragments arranged by size: 

                3313    2069     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5630
 
SmaI CCC'GGG

Cuts at:   1106    2652    1106 
   Size:       1546    4084
 
SmlI C'TyrA_G

Cuts at:   1369    2785    3700    4192    4454    1369 
   Size:       1416     915     492     262    2545

  Fragments arranged by size: 

                2545    1416     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5630
 
SphI G_CATG'C

Cuts at:   2199    2683    3183    2199 
   Size:        484     500    4646

  Fragments arranged by size: 

                4646     500     484
 
StuI AGG'CCT

Cuts at:   1717    1717 
   Size:       5630
 
StyI C'CwwG_G

Cuts at:    107     686    1982    2764    3118     107 
   Size:        579    1296     782     354    2619

  Fragments arranged by size: 

                2619    1296     782     579     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    202     675    1815    1902    3213    3346    3805    4014     202 
   Size:        473    1140      87    1311     133     459     209    1818

  Fragments arranged by size: 

                1818    1311    1140     473     459     209     133      87
            
 
TatI w'GTAC_w

Cuts at:   1271    5145    1271 
   Size:       3874    1756
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5147    5179    5147 
   Size:         32    5598
 
Tth111I GACn'n_nGTC

Cuts at:   1030    3427    1030 
   Size:       2397    3233
 
XbaI T'CTAG_A

Cuts at:   2661    2661 
   Size:       5630
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    985    1809    3125     985 
   Size:        824    1316    3490

  Fragments arranged by size: 

                3490    1316     824
 
XmaI C'CCGG_G

Cuts at:   1104    2650    1104 
   Size:       1546    4084
 
XmnI GAAnn'nnTTC

Cuts at:   1489    1489 
   Size:       5630
 
ZraI GAC'GTC

Cuts at:    857     857 
   Size:       5630
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AloI     AscI    AvrII     BaeI     BcgI
    BfrBI     BmtI    BsiWI    BsrGI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI
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