Restriction map for pF1KB7319
 (Linear) (Six-base) MAPSORT of: KIBB7319.seq  Check: 7506  from: 1  to: 2517



KIBB7319.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     153    1234    1894    2517 
   Size:        153    1081     660     623

  Fragments arranged by size: 

                1081     660     623     153
 
AatII G_ACGT'C

Cuts at:      0     751    2517 
   Size:        751    1766
 
AccI GT'mk_AC

Cuts at:      0    1154    1244    2517 
   Size:       1154      90    1273

  Fragments arranged by size: 

                1273    1154      90
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    1444    1607    2517 
   Size:        987     457     163     910

  Fragments arranged by size: 

                 987     910     457     163
 
AleI CACnn'nnGTG

Cuts at:      0    2368    2517 
   Size:       2368     149
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     280    2197    2269    2517 
   Size:        280    1917      72     248

  Fragments arranged by size: 

                1917     280     248      72
 
AlwNI CAG_nnn'CTG

Cuts at:      0     768    2012    2517 
   Size:        768    1244     505

  Fragments arranged by size: 

                1244     768     505
 
ApaI G_GGCC'C

Cuts at:      0       8    1027    1804    2033    2517 
   Size:          8    1019     777     229     484

  Fragments arranged by size: 

                1019     777     484     229       8
 
AvaI C'yCGr_G

Cuts at:      0     996    2517 
   Size:        996    1521
 
BanI G'GyrC_C

Cuts at:      0     165     883     958    1493    1567    1689    2517 
   Size:        165     718      75     535      74     122     828

  Fragments arranged by size: 

                 828     718     535     165     122      75      74
 
BanII G_rGCy'C

Cuts at:      0       8    1027    1804    2033    2517 
   Size:          8    1019     777     229     484

  Fragments arranged by size: 

                1019     777     484     229       8
 
BbeI G_GCGC'C

Cuts at:      0     887    2517 
   Size:        887    1630
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1662    2517 
   Size:       1662     855
 
BbvCI CC'TCA_GC

Cuts at:      0    1048    1732    2290    2517 
   Size:       1048     684     558     227

  Fragments arranged by size: 

                1048     684     558     227
 
BglI GCCn_nnn'nGGC

Cuts at:      0     956    1208    1565    1714    2517 
   Size:        956     252     357     149     803

  Fragments arranged by size: 

                 956     803     357     252     149
 
BglII A'GATC_T

Cuts at:      0    1268    2517 
   Size:       1268    1249
 
BlpI GC'TnA_GC

Cuts at:      0      56    2517 
   Size:         56    2461
 
Bme1580I G_kGCm'C

Cuts at:      0       8     170     961     992    1027    1804    2033    2517 
   Size:          8     162     791      31      35     777     229     484

  Fragments arranged by size: 

                 791     777     484     229     162      35      31       8
            
 
BmgBI CAC'GTC

Cuts at:      0    1329    1533    2517 
   Size:       1329     204     984

  Fragments arranged by size: 

                1329     984     204
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2022    2517 
   Size:       2022     495
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1782    2517 
   Size:       1782     735
 
Bpu10I CC'TnA_GC

Cuts at:      0    1048    1732    2290    2517 
   Size:       1048     684     558     227

  Fragments arranged by size: 

                1048     684     558     227
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1282    2517 
   Size:       1282    1235
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1636    1884    2517 
   Size:       1636     248     633

  Fragments arranged by size: 

                1636     633     248
 
BsaAI yAC'GTr

Cuts at:      0    1863    2517 
   Size:       1863     654
 
BsaHI Gr'CG_yC

Cuts at:      0     748     884    2517 
   Size:        748     136    1633

  Fragments arranged by size: 

                1633     748     136
 
BsaWI w'CCGG_w

Cuts at:      0    1367    2517 
   Size:       1367    1150
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475    2517 
   Size:        445      30    2042

  Fragments arranged by size: 

                2042     445      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      15     253     769    1195    1279    2517 
   Size:         15     238     516     426      84    1238

  Fragments arranged by size: 

                1238     516     426     238      84      15
 
BseYI C'CCAG_C

Cuts at:      0     279     553    1033    1795    2517 
   Size:        279     274     480     762     722

  Fragments arranged by size: 

                 762     722     480     279     274
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     604     913    1180    1212    1321    2313    2517 
   Size:        580      24     309     267      32     109     992     204

  Fragments arranged by size: 

                 992     580     309     267     204     109      32      24
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     335     802    2393    2517 
   Size:        335     467    1591     124

  Fragments arranged by size: 

                1591     467     335     124
 
BsmI GAATG_Cn'

Cuts at:      0    1606    2517 
   Size:       1606     911
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     706    2043    2325    2517 
   Size:        706    1337     282     192

  Fragments arranged by size: 

                1337     706     282     192
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     170     335     802     961     992    1027    1804 
   Size:          8     162     165     467     159      31      35     777

Cuts at:   1804    2033    2393    2517 
   Size:        229     360     124

  Fragments arranged by size: 

                 777     467     360     229     165     162     159     124
                  35      31       8
 
BspHI T'CATG_A

Cuts at:      0     440    2517 
   Size:        440    2077
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      92     153    1081    1234    1894    2213    2517 
   Size:         92      61     928     153     660     319     304

  Fragments arranged by size: 

                 928     660     319     304     153      92      61
 
BsrDI GCAATG_nn'

Cuts at:      0    1366    2517 
   Size:       1366    1151
 
BsrFI r'CCGG_y

Cuts at:      0      60     327    2517 
   Size:         60     267    2190

  Fragments arranged by size: 

                2190     267      60
 
BssHII G'CGCG_C

Cuts at:      0     805    2517 
   Size:        805    1712
 
BssSI C'ACGA_G

Cuts at:      0     349    2517 
   Size:        349    2168
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     222     591    1556    2517 
   Size:        222     369     965     961

  Fragments arranged by size: 

                 965     961     369     222
 
BstEII G'GTnAC_C

Cuts at:      0     771    2361    2517 
   Size:        771    1590     156

  Fragments arranged by size: 

                1590     771     156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     667    1239    1704    2412    2517 
   Size:        667     572     465     708     105

  Fragments arranged by size: 

                 708     667     572     465     105
 
BstYI r'GATC_y

Cuts at:      0    1268    2517 
   Size:       1268    1249
 
BtgI C'CryG_G

Cuts at:      0     578    2096    2517 
   Size:        578    1518     421

  Fragments arranged by size: 

                1518     578     421
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     518     684    1683    2517 
   Size:        518     166     999     834

  Fragments arranged by size: 

                 999     834     518     166
 
BtsI GCAGTG_nn'

Cuts at:      0     724    1825    2517 
   Size:        724    1101     692

  Fragments arranged by size: 

                1101     724     692
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2380    2517 
   Size:       2380     137
 
EaeI y'GGCC_r

Cuts at:      0     449     645     657    1715    1742    1982    2093    2402 
   Size:        449     196      12    1058      27     240     111     309

Cuts at:   2402    2517 
   Size:        115

  Fragments arranged by size: 

                1058     449     309     240     196     115     111      27
                  12
 
EarI CTCTTCn'nnn_

Cuts at:      0     118    2452    2517 
   Size:        118    2334      65

  Fragments arranged by size: 

                2334     118      65
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    1444    1607    1782    2517 
   Size:        987     457     163     175     735

  Fragments arranged by size: 

                 987     735     457     175     163
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     214    2517 
   Size:        214    2303
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4      48    1023    1800    1801    1902    2029    2030 
   Size:          4      44     975     777       1     101     127       1

Cuts at:   2030    2517 
   Size:        487

  Fragments arranged by size: 

                 975     777     487     127     101      44       4       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     543    1299    2517 
   Size:        543     756    1218

  Fragments arranged by size: 

                1218     756     543
 
HaeII r_GCGC'y

Cuts at:      0     497     560     762     768     887    2517 
   Size:        497      63     202       6     119    1630

  Fragments arranged by size: 

                1630     497     202     119      63       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1362    1394    2517 
   Size:       1362      32    1123

  Fragments arranged by size: 

                1362    1123      32
 
HincII GTy'rAC

Cuts at:      0    1281    2517 
   Size:       1281    1236
 
KasI G'GCGC_C

Cuts at:      0     883    2517 
   Size:        883    1634
 
MfeI C'AATT_G

Cuts at:      0    2147    2517 
   Size:       2147     370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    2517 
   Size:        338    2179
 
MscI TGG'CCA

Cuts at:      0     451     659    1744    1984    2095    2404    2517 
   Size:        451     208    1085     240     111     309     113

  Fragments arranged by size: 

                1085     451     309     240     208     113     111
 
MslI CAynn'nnrTG

Cuts at:      0     445    1702    1750    2335    2368    2517 
   Size:        445    1257      48     585      33     149

  Fragments arranged by size: 

                1257     585     445     149      48      33
 
MspA1I CmG'CkG

Cuts at:      0     588     605     663    1257    1422    1665    2351    2517 
   Size:        588      17      58     594     165     243     686     166

  Fragments arranged by size: 

                 686     594     588     243     166     165      58      17
            
 
NaeI GCC'GGC

Cuts at:      0      62    2517 
   Size:         62    2455
 
NarI GG'CG_CC

Cuts at:      0     884    2517 
   Size:        884    1633
 
NcoI C'CATG_G

Cuts at:      0     578    2517 
   Size:        578    1939
 
NgoMIV G'CCGG_C

Cuts at:      0      60    2517 
   Size:         60    2457
 
NspI r_CATG'y

Cuts at:      0    2091    2517 
   Size:       2091     426
 
PasI CC'CwG_GG

Cuts at:      0       8    1722    2517 
   Size:          8    1714     795

  Fragments arranged by size: 

                1714     795       8
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     768    1033    2517 
   Size:        768     265    1484

  Fragments arranged by size: 

                1484     768     265
 
PfoI T'CCnGG_A

Cuts at:      0     707    1292    1673    2517 
   Size:        707     585     381     844

  Fragments arranged by size: 

                 844     707     585     381
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2263    2295    2517 
   Size:       2263      32     222

  Fragments arranged by size: 

                2263     222      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      48    2517 
   Size:         48    2469
 
PspOMI G'GGCC_C

Cuts at:      0       4    1023    1800    2029    2517 
   Size:          4    1019     777     229     488

  Fragments arranged by size: 

                1019     777     488     229       4
 
PstI C_TGCA'G

Cuts at:      0     164     731     794    1415    2517 
   Size:        164     567      63     621    1102

  Fragments arranged by size: 

                1102     621     567     164      63
 
PvuII CAG'CTG

Cuts at:      0     588     663    1257    1422    1665    2517 
   Size:        588      75     594     165     243     852

  Fragments arranged by size: 

                 852     594     588     243     165      75
 
SbfI CC_TGCA'GG

Cuts at:      0     164    2517 
   Size:        164    2353
 
SfcI C'TryA_G

Cuts at:      0     160     381     489     727     790    1155    1411    2004 
   Size:        160     221     108     238      63     365     256     593

Cuts at:   2004    2346    2517 
   Size:        342     171

  Fragments arranged by size: 

                 593     365     342     256     238     221     171     160
                 108      63
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1714    2517 
   Size:       1714     803
 
SfoI GGC'GCC

Cuts at:      0     885    2517 
   Size:        885    1632
 
SmaI CCC'GGG

Cuts at:      0     998    2517 
   Size:        998    1519
 
SmlI C'TyrA_G

Cuts at:      0    1261    2517 
   Size:       1261    1256
 
SphI G_CATG'C

Cuts at:      0    2091    2517 
   Size:       2091     426
 
StuI AGG'CCT

Cuts at:      0    1609    2517 
   Size:       1609     908
 
StyI C'CwwG_G

Cuts at:      0     578    1874    2517 
   Size:        578    1296     643

  Fragments arranged by size: 

                1296     643     578
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      94     567    1707    1794    2517 
   Size:         94     473    1140      87     723

  Fragments arranged by size: 

                1140     723     473      94      87
 
TatI w'GTAC_w

Cuts at:      0    1163    2517 
   Size:       1163    1354
 
Tth111I GACn'n_nGTC

Cuts at:      0     922    2517 
   Size:        922    1595
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     877    1701    2517 
   Size:        877     824     816

  Fragments arranged by size: 

                 877     824     816
 
XmaI C'CCGG_G

Cuts at:      0     996    2517 
   Size:        996    1521
 
XmnI GAAnn'nnTTC

Cuts at:      0    1381    2517 
   Size:       1381    1136
 
ZraI GAC'GTC

Cuts at:      0     749    2517 
   Size:        749    1768
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AleI     AlfI    AlwNI     ApaI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SbfI     SfcI     SfiI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BciVI     BclI    BfrBI     BmtI     BplI    BsaBI    BsiEI
    BsiWI    BspEI    BsrBI    BsrGI    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI     TstI     XbaI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com