Restriction map for pF1KB6531
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstUI       
BstZ17I     CfoI        ClaI        CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          132  GACGCC               G[GA]^CG[CT]C
Alw44I        673  GTGCAC               G^TGCAC
AlwI          825  GATCC                GGATC(4/5)
AocI          355  CCTGAGG              CC^T[ACGT]AGG
ApaLI         673  GTGCAC               G^TGCAC
AvaI          695  CCCGGG               C^[CT]CG[GA]G
AvaII         116  GGACC                G^G[AT]CC
BlpI          948  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      948  GCTGAGC              GC^T[ACGT]AGC
BsaHI         132  GACGCC               G[GA]^CG[CT]C
BsaWI         366  TCCGGT               [AT]^CCGG[AT]
BsmAI         451  GTCTC                GTCTC(1/5)
BsmI          645  GAATGC               GAATGC(1/-1)
BsoBI         695  CCCGGG               C^[CT]CG[GA]G
BsrDI         704  GCAATG               GCAATG(2/0)
BstBI         767  TTCGAA               TT^CGAA
BstEII        195  GGTCACC              G^GT[ACGT]ACC
BstPI         195  GGTCACC              G^GT[ACGT]ACC
BstYI         824  AGATCC               [GA]^GATC[CT]
Bsu36I        355  CCTGAGG              CC^T[ACGT]AGG
CelII         948  GCTGAGC              GC^T[ACGT]AGC
DrdI          540  GACCCAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        355  CCTGAGG              CC^T[ACGT]AGG
EcoNI          99  CCTTCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       195  GGTCACC              G^GT[ACGT]ACC
EcoRI         571  GAATTC               G^AATTC
EcoT14I       431  CCATGG               C^C[AT][AT]GG
HgaI          132  GACGC                GACGC(5/10)
Hin1I         132  GACGCC               G[GA]^CG[CT]C
MaeII         923  ACGT                 A^CGT
MflI          824  AGATCC               [GA]^GATC[CT]
MslI          738  CATTGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          431  CCATGG               C^CATGG
NspI          296  GCATGC               [GA]CATG^[CT]
NspV          767  TTCGAA               TT^CGAA
PflMI         969  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          898  GAGCTC               GAGCT^C
SfaNI         691  GATGC                GCATC(5/9)
SfuI          767  TTCGAA               TT^CGAA
SmaI          695  CCCGGG               CCC^GGG
SmlI         1075  CTCAAG               C^T[CT][GA]AG
SphI          296  GCATGC               GCATG^C
StuI          914  AGGCCT               AGG^CCT
StyI          431  CCATGG               C^C[AT][AT]GG
TfiI          796  GATTC                G^A[AT]TC
Van91I        969  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         824  AGATCC               [GA]^GATC[CT]
XmaCI         695  CCCGGG               C^CCGGG
XmaI          695  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          571  GAATTC               [GA]^AATT[CT]
              807  AAATTC              
ApoI          571  GAATTC               [GA]^AATT[CT]
              807  AAATTC              
BanI          681  GGTGCC               G^G[CT][GA]CC
              862  GGTGCC              
BbsI          129  GAAGAC               GAAGAC(2/6)
              764  GTCTTC              
BbvCI         457  CCTCAGC              CCTCAGC(-5/-2)
              262  GCTGAGG             
BfaI           50  CTAG                 C^TAG
              340  CTAG                
BpmI          301  CTGGAG               CTGGAG(16/14)
              313  CTGGAG              
BpuAI         129  GAAGAC               GAAGAC(2/6)
              764  GTCTTC              
BsgI          216  CTGCAC               GTGCAG(16/14)
              505  GTGCAG              
BsmFI         115  GGGAC                GGGAC(10/14)
              831  GTCCC               
BsrFI         306  GCCGGC               [GA]^CCGG[CT]
              996  GCCGGC              
BstAPI        382  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              675  GCACACGGTGC         
Cfr10I        306  GCCGGC               [GA]^CCGG[CT]
              996  GCCGGC              
DraIII        676  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1002  CACTGTGTG           
EarI          345  CTCTTC               CTCTTC(1/4)
                7  GAAGAG              
Ksp632I       345  CTCTTC               CTCTTC(1/4)
                7  GAAGAG              
MaeI           50  CTAG                 C^TAG
              340  CTAG                
MaeIII        196  GTCAC                ^GT[ACGT]AC
              538  GTGAC               
NaeI          306  GCCGGC               GCC^GGC
              996  GCCGGC              
NgoMIV        306  GCCGGC               G^CCGGC
              996  GCCGGC              
Tsp45I        196  GTCAC                ^GT[GC]AC
              538  GTGAC               
XcmI          729  CCACTTCTCCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              781  CCAGGTTGCACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       ApaI        
AspHI       BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TseI        
Tsp509I     TspRI       TthHB8I     
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