Restriction map for pF1KB6531
 (Linear) (Six-base) MAPSORT of: KIBB6531.seq  Check: 5034  from: 1  to: 1098



KIBB6531.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     388     498     975    1098 
   Size:        388     110     477     123

  Fragments arranged by size: 

                 477     388     123     110
 
ApaI G_GGCC'C

Cuts at:      0     232     781     874    1098 
   Size:        232     549      93     224

  Fragments arranged by size: 

                 549     232     224      93
 
ApaLI G'TGCA_C

Cuts at:      0     673    1098 
   Size:        673     425
 
ApoI r'AATT_y

Cuts at:      0     571     807    1098 
   Size:        571     236     291

  Fragments arranged by size: 

                 571     291     236
 
AvaI C'yCGr_G

Cuts at:      0     695    1098 
   Size:        695     403
 
BanI G'GyrC_C

Cuts at:      0     681     862    1098 
   Size:        681     181     236

  Fragments arranged by size: 

                 681     236     181
 
BanII G_rGCy'C

Cuts at:      0     232     427     751     781     856     874     902    1098 
   Size:        232     195     324      30      75      18      28     196

  Fragments arranged by size: 

                 324     232     196     195      75      30      28      18
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     136     757    1098 
   Size:        136     621     341

  Fragments arranged by size: 

                 621     341     136
 
BbvCI CC'TCA_GC

Cuts at:      0     263     458    1098 
   Size:        263     195     640

  Fragments arranged by size: 

                 640     263     195
 
BlpI GC'TnA_GC

Cuts at:      0     949    1098 
   Size:        949     149
 
Bme1580I G_kGCm'C

Cuts at:      0     232     677     781     867     874    1065    1098 
   Size:        232     445     104      86       7     191      33

  Fragments arranged by size: 

                 445     232     191     104      86      33       7
 
BmgBI CAC'GTC

Cuts at:      0     924    1098 
   Size:        924     174
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     391    1098 
   Size:        391     707
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      36    1098 
   Size:         36    1062
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     334    1098 
   Size:        322      12     764

  Fragments arranged by size: 

                 764     322      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     263     458    1098 
   Size:        263     195     640

  Fragments arranged by size: 

                 640     263     195
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1060    1098 
   Size:       1060      38
 
BsaHI Gr'CG_yC

Cuts at:      0     133    1098 
   Size:        133     965
 
BsaWI w'CCGG_w

Cuts at:      0     366    1098 
   Size:        366     732
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     718     748    1098 
   Size:        718      30     350

  Fragments arranged by size: 

                 718     350      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     922     958    1098 
   Size:         25     897      36     140

  Fragments arranged by size: 

                 897     140      36      25
 
BseYI C'CCAG_C

Cuts at:      0     232     992    1098 
   Size:        232     760     106

  Fragments arranged by size: 

                 760     232     106
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     526    1098 
   Size:        201     325     572

  Fragments arranged by size: 

                 572     325     201
 
BsiHKAI G_wGCw'C

Cuts at:      0     489     677     902    1098 
   Size:        489     188     225     196

  Fragments arranged by size: 

                 489     225     196     188
 
BsmI GAATG_Cn'

Cuts at:      0     651    1098 
   Size:        651     447
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     427     489     677     751     781     856     867 
   Size:        232     195      62     188      74      30      75      11

Cuts at:    867     874     902    1065    1098 
   Size:          7      28     163      33

  Fragments arranged by size: 

                 232     195     188     163      75      74      62      33
                  30      28      11       7
 
BsrDI GCAATG_nn'

Cuts at:      0     711    1098 
   Size:        711     387
 
BsrFI r'CCGG_y

Cuts at:      0     306     996    1098 
   Size:        306     690     102

  Fragments arranged by size: 

                 690     306     102
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     388     681    1098 
   Size:        388     293     417

  Fragments arranged by size: 

                 417     388     293
 
BstBI TT'CG_AA

Cuts at:      0     768    1098 
   Size:        768     330
 
BstEII G'GTnAC_C

Cuts at:      0     195    1098 
   Size:        195     903
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     550     633     744    1098 
   Size:        550      83     111     354

  Fragments arranged by size: 

                 550     354     111      83
 
BstYI r'GATC_y

Cuts at:      0     824    1098 
   Size:        824     274
 
Bsu36I CC'TnA_GG

Cuts at:      0     356    1098 
   Size:        356     742
 
BtgI C'CryG_G

Cuts at:      0      32     431    1041    1098 
   Size:         32     399     610      57

  Fragments arranged by size: 

                 610     399      57      32
 
BtsI GCAGTG_nn'

Cuts at:      0     349    1098 
   Size:        349     749
 
DraIII CAC_nnn'GTG

Cuts at:      0     681    1007    1098 
   Size:        681     326      91

  Fragments arranged by size: 

                 681     326      91
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     546    1098 
   Size:        546     552
 
EaeI y'GGCC_r

Cuts at:      0      68     653     998    1098 
   Size:         68     585     345     100

  Fragments arranged by size: 

                 585     345     100      68
 
EarI CTCTTCn'nnn_

Cuts at:      0       2     351    1098 
   Size:          2     349     747

  Fragments arranged by size: 

                 747     349       2
 
EcoICRI GAG'CTC

Cuts at:      0     900    1098 
   Size:        900     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     334    1098 
   Size:        322      12     764

  Fragments arranged by size: 

                 764     322      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     103    1098 
   Size:        103     995
 
EcoO109I rG'GnC_Cy

Cuts at:      0     228     229     777     871    1098 
   Size:        228       1     548      94     227

  Fragments arranged by size: 

                 548     228     227      94       1
 
EcoRI G'AATT_C

Cuts at:      0     571    1098 
   Size:        571     527
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     718     750     809     841    1098 
   Size:        718      32      59      32     257

  Fragments arranged by size: 

                 718     257      59      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     742    1098 
   Size:        742     356
 
MspA1I CmG'CkG

Cuts at:      0     215     262     388    1098 
   Size:        215      47     126     710

  Fragments arranged by size: 

                 710     215     126      47
 
NaeI GCC'GGC

Cuts at:      0     308     998    1098 
   Size:        308     690     100

  Fragments arranged by size: 

                 690     308     100
 
NcoI C'CATG_G

Cuts at:      0     431    1098 
   Size:        431     667
 
NgoMIV G'CCGG_C

Cuts at:      0     306     996    1098 
   Size:        306     690     102

  Fragments arranged by size: 

                 690     306     102
 
NspI r_CATG'y

Cuts at:      0     300    1098 
   Size:        300     798
 
PasI CC'CwG_GG

Cuts at:      0     849     858     867    1098 
   Size:        849       9       9     231

  Fragments arranged by size: 

                 849     231       9       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     975    1098 
   Size:        975     123
 
PspOMI G'GGCC_C

Cuts at:      0     228     777     870    1098 
   Size:        228     549      93     228

  Fragments arranged by size: 

                 549     228     228      93
 
PstI C_TGCA'G

Cuts at:      0     107     384     443     494    1070    1098 
   Size:        107     277      59      51     576      28

  Fragments arranged by size: 

                 576     277     107      59      51      28
 
PvuII CAG'CTG

Cuts at:      0     215     262     388    1098 
   Size:        215      47     126     710

  Fragments arranged by size: 

                 710     215     126      47
 
SacI G_AGCT'C

Cuts at:      0     902    1098 
   Size:        902     196
 
SfcI C'TryA_G

Cuts at:      0     103     380     407     439     490    1066    1098 
   Size:        103     277      27      32      51     576      32

  Fragments arranged by size: 

                 576     277     103      51      32      32      27
 
SmaI CCC'GGG

Cuts at:      0     697    1098 
   Size:        697     401
 
SmlI C'TyrA_G

Cuts at:      0    1075    1098 
   Size:       1075      23
 
SphI G_CATG'C

Cuts at:      0     300    1098 
   Size:        300     798
 
StuI AGG'CCT

Cuts at:      0     916    1098 
   Size:        916     182
 
StyI C'CwwG_G

Cuts at:      0     431    1098 
   Size:        431     667
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     850    1006    1098 
   Size:        850     156      92

  Fragments arranged by size: 

                 850     156      92
 
TatI w'GTAC_w

Cuts at:      0     530    1098 
   Size:        530     568
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     736     788    1098 
   Size:        736      52     310

  Fragments arranged by size: 

                 736     310      52
 
XmaI C'CCGG_G

Cuts at:      0     695    1098 
   Size:        695     403
 

 Enzymes that do cut:

    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbsI
    BbvCI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BsrFI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    Hin4I     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI   PspOMI     PstI
    PvuII     SacI     SfcI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BmtI     BsaI    BsaAI    BsaBI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI  BstZ17I    BtgZI     ClaI    CspCI     DraI     EagI     EciI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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