Restriction map for pF1KB6374
 (Linear) (Six-base) MAPSORT of: KIBB6374.seq  Check: 5084  from: 1  to: 828



KIBB6374.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     263     286     828 
   Size:         48     215      23     542

  Fragments arranged by size: 

                 542     215      48      23
 
AflIII A'CryG_T

Cuts at:      0      20     828 
   Size:         20     808
 
ApaI G_GGCC'C

Cuts at:      0      41      50     828 
   Size:         41       9     778

  Fragments arranged by size: 

                 778      41       9
 
ApaLI G'TGCA_C

Cuts at:      0     682     828 
   Size:        682     146
 
ApoI r'AATT_y

Cuts at:      0     132     828 
   Size:        132     696
 
AvaI C'yCGr_G

Cuts at:      0      33      42     828 
   Size:         33       9     786

  Fragments arranged by size: 

                 786      33       9
 
BanII G_rGCy'C

Cuts at:      0      41      50     828 
   Size:         41       9     778

  Fragments arranged by size: 

                 778      41       9
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     766     828 
   Size:        766      62
 
BglI GCCn_nnn'nGGC

Cuts at:      0      45     828 
   Size:         45     783
 
Bme1580I G_kGCm'C

Cuts at:      0      41      50     686     828 
   Size:         41       9     636     142

  Fragments arranged by size: 

                 636     142      41       9
 
BmgBI CAC'GTC

Cuts at:      0      83     687     828 
   Size:         83     604     141

  Fragments arranged by size: 

                 604     141      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     828 
   Size:        592     236
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     828 
   Size:        150     678
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     462     828 
   Size:        462     366
 
BsaBI GATnn'nnATC

Cuts at:      0     593     754     828 
   Size:        593     161      74

  Fragments arranged by size: 

                 593     161      74
 
BsaWI w'CCGG_w

Cuts at:      0      26     828 
   Size:         26     802
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     460     490     828 
   Size:        460      30     338

  Fragments arranged by size: 

                 460     338      30
 
BseYI C'CCAG_C

Cuts at:      0      50     828 
   Size:         50     778
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     379     828 
   Size:        105     274     449

  Fragments arranged by size: 

                 449     274     105
 
BsiHKAI G_wGCw'C

Cuts at:      0     686     828 
   Size:        686     142
 
Bsp1286I G_dGCh'C

Cuts at:      0      41      50     686     828 
   Size:         41       9     636     142

  Fragments arranged by size: 

                 636     142      41       9
 
BspHI T'CATG_A

Cuts at:      0     317     828 
   Size:        317     511
 
BstYI r'GATC_y

Cuts at:      0     412     828 
   Size:        412     416
 
BtsI GCAGTG_nn'

Cuts at:      0     116     828 
   Size:        116     712
 
DraI TTT'AAA

Cuts at:      0     606     828 
   Size:        606     222
 
EaeI y'GGCC_r

Cuts at:      0     512     614     828 
   Size:        512     102     214

  Fragments arranged by size: 

                 512     214     102
 
EarI CTCTTCn'nnn_

Cuts at:      0     425     828 
   Size:        425     403
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     263     286     592     828 
   Size:         48     215      23     306     236

  Fragments arranged by size: 

                 306     236     215      48      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0      37      38      46      47     254     828 
   Size:         37       1       8       1     207     574

  Fragments arranged by size: 

                 574     207      37       8       1       1
 
HaeII r_GCGC'y

Cuts at:      0     291     828 
   Size:        291     537
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     788     820     828 
   Size:        788      32       8

  Fragments arranged by size: 

                 788      32       8
 
MscI TGG'CCA

Cuts at:      0     514     616     828 
   Size:        514     102     212

  Fragments arranged by size: 

                 514     212     102
 
MspA1I CmG'CkG

Cuts at:      0      89     102     453     570     828 
   Size:         89      13     351     117     258

  Fragments arranged by size: 

                 351     258     117      89      13
 
NspI r_CATG'y

Cuts at:      0      24     828 
   Size:         24     804
 
PciI A'CATG_T

Cuts at:      0      20     828 
   Size:         20     808
 
PfoI T'CCnGG_A

Cuts at:      0      56     828 
   Size:         56     772
 
PpuMI rG'GwC_Cy

Cuts at:      0     254     828 
   Size:        254     574
 
PsiI TTA'TAA

Cuts at:      0     818     828 
   Size:        818      10
 
PspOMI G'GGCC_C

Cuts at:      0      37      46     828 
   Size:         37       9     782

  Fragments arranged by size: 

                 782      37       9
 
PstI C_TGCA'G

Cuts at:      0     336     429     828 
   Size:        336      93     399

  Fragments arranged by size: 

                 399     336      93
 
PvuII CAG'CTG

Cuts at:      0     453     570     828 
   Size:        453     117     258

  Fragments arranged by size: 

                 453     258     117
 
SfcI C'TryA_G

Cuts at:      0     175     332     425     828 
   Size:        175     157      93     403

  Fragments arranged by size: 

                 403     175     157      93
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      45     828 
   Size:         45     783
 
SmaI CCC'GGG

Cuts at:      0      35     828 
   Size:         35     793
 
SmlI C'TyrA_G

Cuts at:      0     165     828 
   Size:        165     663
 
StyI C'CwwG_G

Cuts at:      0     221     828 
   Size:        221     607
 
XmaI C'CCGG_G

Cuts at:      0      33     828 
   Size:         33     795
 
XmnI GAAnn'nnTTC

Cuts at:      0     325     828 
   Size:        325     503
 

 Enzymes that do cut:

     AcuI   AflIII     ApaI    ApaLI     ApoI     AvaI    BanII    BciVI
     BglI Bme1580I    BmgBI     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BstYI     BtsI
     DraI     EaeI     EarI  Eco57MI EcoO109I    HaeII    Hin4I     MscI
   MspA1I     NspI     PciI     PfoI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SfcI     SfiI     SmaI     SmlI     StyI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com