Restriction map for pF1KB7244
 (Linear) (Six-base) MAPSORT of: KIBB7244.seq  Check: 2703  from: 1  to: 1092



KIBB7244.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AatII G_ACGT'C

Cuts at:      0     260    1092 
   Size:        260     832
 
Acc65I G'GTAC_C

Cuts at:      0     550    1092 
   Size:        550     542
 
AleI CACnn'nnGTG

Cuts at:      0     616    1092 
   Size:        616     476
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     529    1092 
   Size:        529     563
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27      28      60     289     321    1092 
   Size:         27       1      32     229      32     771

  Fragments arranged by size: 

                 771     229      32      32      27       1
 
ApaI G_GGCC'C

Cuts at:      0     114    1092 
   Size:        114     978
 
ApaLI G'TGCA_C

Cuts at:      0     424    1092 
   Size:        424     668
 
AvaI C'yCGr_G

Cuts at:      0      48     113    1092 
   Size:         48      65     979

  Fragments arranged by size: 

                 979      65      48
 
BamHI G'GATC_C

Cuts at:      0    1016    1092 
   Size:       1016      76
 
BanI G'GyrC_C

Cuts at:      0      96     126     449     550     592     976    1092 
   Size:         96      30     323     101      42     384     116

  Fragments arranged by size: 

                 384     323     116     101      96      42      30
 
BanII G_rGCy'C

Cuts at:      0       8     114     439     578     839     973    1092 
   Size:          8     106     325     139     261     134     119

  Fragments arranged by size: 

                 325     261     139     134     119     106       8
 
BbeI G_GCGC'C

Cuts at:      0     100     453     596    1092 
   Size:        100     353     143     496

  Fragments arranged by size: 

                 496     353     143     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     220     372     591    1092 
   Size:        220     152     219     501

  Fragments arranged by size: 

                 501     220     219     152
 
BglI GCCn_nnn'nGGC

Cuts at:      0     472     582    1092 
   Size:        472     110     510

  Fragments arranged by size: 

                 510     472     110
 
BlpI GC'TnA_GC

Cuts at:      0     433    1092 
   Size:        433     659
 
Bme1580I G_kGCm'C

Cuts at:      0     114     131     428    1092 
   Size:        114      17     297     664

  Fragments arranged by size: 

                 664     297     114      17
 
BmgBI CAC'GTC

Cuts at:      0     537    1092 
   Size:        537     555
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     858    1092 
   Size:        858     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1092 
   Size:         83    1009
 
BsaAI yAC'GTr

Cuts at:      0     189    1092 
   Size:        189     903
 
BsaHI Gr'CG_yC

Cuts at:      0      97     257     359     450     593     992    1092 
   Size:         97     160     102      91     143     399     100

  Fragments arranged by size: 

                 399     160     143     102     100      97      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25      28      58    1092 
   Size:         25       3      30    1034

  Fragments arranged by size: 

                1034      30      25       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      33    1092 
   Size:         33    1059
 
BseYI C'CCAG_C

Cuts at:      0      15      28     162     270     621    1092 
   Size:         15      13     134     108     351     471

  Fragments arranged by size: 

                 471     351     134     108      15      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559    1092 
   Size:        559     533
 
BsiEI CG_ry'CG

Cuts at:      0     158     617    1092 
   Size:        158     459     475

  Fragments arranged by size: 

                 475     459     158
 
BsiHKAI G_wGCw'C

Cuts at:      0     428     439    1092 
   Size:        428      11     653

  Fragments arranged by size: 

                 653     428      11
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     114     131     428     439     578     839     973 
   Size:          8     106      17     297      11     139     261     134

Cuts at:    973    1092 
   Size:        119

  Fragments arranged by size: 

                 297     261     139     134     119     106      17      11
                   8
 
BspHI T'CATG_A

Cuts at:      0     344     395    1092 
   Size:        344      51     697

  Fragments arranged by size: 

                 697     344      51
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     149     562     671    1092 
   Size:        149     413     109     421

  Fragments arranged by size: 

                 421     413     149     109
 
BsrBI CCG'CTC

Cuts at:      0     519     729    1092 
   Size:        519     210     363

  Fragments arranged by size: 

                 519     363     210
 
BsrFI r'CCGG_y

Cuts at:      0      93     847    1092 
   Size:         93     754     245

  Fragments arranged by size: 

                 754     245      93
 
BsrGI T'GTAC_A

Cuts at:      0     263    1092 
   Size:        263     829
 
BstYI r'GATC_y

Cuts at:      0    1016    1092 
   Size:       1016      76
 
BtgI C'CryG_G

Cuts at:      0     482     578     818     995    1092 
   Size:        482      96     240     177      97

  Fragments arranged by size: 

                 482     240     177      97      96
 
BtsI GCAGTG_nn'

Cuts at:      0     254     425    1092 
   Size:        254     171     667

  Fragments arranged by size: 

                 667     254     171
 
EaeI y'GGCC_r

Cuts at:      0     155     251     287     464     568     614     815    1092 
   Size:        155      96      36     177     104      46     201     277

  Fragments arranged by size: 

                 277     201     177     155     104      96      46      36
            
 
EagI C'GGCC_G

Cuts at:      0     155     614    1092 
   Size:        155     459     478

  Fragments arranged by size: 

                 478     459     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     834     922    1092 
   Size:        834      88     170

  Fragments arranged by size: 

                 834     170      88
 
EcoICRI GAG'CTC

Cuts at:      0     437    1092 
   Size:        437     655
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1092 
   Size:         83    1009
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     964    1092 
   Size:        964     128
 
EcoO109I rG'GnC_Cy

Cuts at:      0     110     492    1092 
   Size:        110     382     600

  Fragments arranged by size: 

                 600     382     110
 
HaeII r_GCGC'y

Cuts at:      0     100     453     482     596     723     826    1092 
   Size:        100     353      29     114     127     103     266

  Fragments arranged by size: 

                 353     266     127     114     103     100      29
 
HincII GTy'rAC

Cuts at:      0     363     801     810    1092 
   Size:        363     438       9     282

  Fragments arranged by size: 

                 438     363     282       9
 
KasI G'GCGC_C

Cuts at:      0      96     449     592    1092 
   Size:         96     353     143     500

  Fragments arranged by size: 

                 500     353     143      96
 
KpnI G_GTAC'C

Cuts at:      0     554    1092 
   Size:        554     538
 
MscI TGG'CCA

Cuts at:      0     289     466    1092 
   Size:        289     177     626

  Fragments arranged by size: 

                 626     289     177
 
MslI CAynn'nnrTG

Cuts at:      0     187     400     616     673    1092 
   Size:        187     213     216      57     419

  Fragments arranged by size: 

                 419     216     213     187      57
 
MspA1I CmG'CkG

Cuts at:      0      32     432     446     484     566     639     890    1092 
   Size:         32     400      14      38      82      73     251     202

  Fragments arranged by size: 

                 400     251     202      82      73      38      32      14
            
 
NaeI GCC'GGC

Cuts at:      0      95     849    1092 
   Size:         95     754     243

  Fragments arranged by size: 

                 754     243      95
 
NarI GG'CG_CC

Cuts at:      0      97     450     593    1092 
   Size:         97     353     143     499

  Fragments arranged by size: 

                 499     353     143      97
 
NgoMIV G'CCGG_C

Cuts at:      0      93     847    1092 
   Size:         93     754     245

  Fragments arranged by size: 

                 754     245      93
 
NspI r_CATG'y

Cuts at:      0     270     510    1092 
   Size:        270     240     582

  Fragments arranged by size: 

                 582     270     240
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27      28      60    1092 
   Size:         27       1      32    1032

  Fragments arranged by size: 

                1032      32      27       1
 
PpuMI rG'GwC_Cy

Cuts at:      0     492    1092 
   Size:        492     600
 
PspOMI G'GGCC_C

Cuts at:      0     110    1092 
   Size:        110     982
 
PvuII CAG'CTG

Cuts at:      0      32     566     890    1092 
   Size:         32     534     324     202

  Fragments arranged by size: 

                 534     324     202      32
 
SacI G_AGCT'C

Cuts at:      0     439    1092 
   Size:        439     653
 
SacII CC_GC'GG

Cuts at:      0     485    1092 
   Size:        485     607
 
SfoI GGC'GCC

Cuts at:      0      98     451     594    1092 
   Size:         98     353     143     498

  Fragments arranged by size: 

                 498     353     143      98
 
SmaI CCC'GGG

Cuts at:      0      50     115    1092 
   Size:         50      65     977

  Fragments arranged by size: 

                 977      65      50
 
SrfI GCCC'GGGC

Cuts at:      0     115    1092 
   Size:        115     977
 
StyI C'CwwG_G

Cuts at:      0     980    1092 
   Size:        980     112
 
TatI w'GTAC_w

Cuts at:      0     263    1092 
   Size:        263     829
 
Tth111I GACn'n_nGTC

Cuts at:      0     358    1092 
   Size:        358     734
 
XmaI C'CCGG_G

Cuts at:      0      48     113    1092 
   Size:         48      65     979

  Fragments arranged by size: 

                 979      65      48
 
ZraI GAC'GTC

Cuts at:      0     258    1092 
   Size:        258     834
 

 Enzymes that do cut:

    AatII   Acc65I     AleI     AlfI     AloI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BglI     BlpI Bme1580I
    BmgBI     BplI     BpmI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BsrGI    BstYI     BtgI     BtsI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII   HincII     KasI     KpnI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PpiI    PpuMI
   PspOMI    PvuII     SacI    SacII     SfoI     SmaI     SrfI     StyI
     TatI  Tth111I     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BmrI     BmtI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI
    BspEI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
  HindIII     HpaI     MfeI     MluI     MmeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmnI
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