Restriction map for pF1KB6445
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
EarI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeIII      MamI        
MflI        MluI        MluNI       MroI        MscI        
MseI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SalI        SapI        SbfI        SfaNI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          414  GGCGTC               G[GA]^CG[CT]C
AflIII        551  ACACGT               A^C[GA][CT]GT
AluI           82  AGCT                 AG^CT
AocI          880  CCTGAGG              CC^T[ACGT]AGG
ApaI          444  GGGCCC               GGGCC^C
BbsI          499  GAAGAC               GAAGAC(2/6)
BbvCI         810  CCTCAGC              CCTCAGC(-5/-2)
BcgI          692  GCAAGTTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI            9  GCCATCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           18  GCTCAGC              GC^T[ACGT]AGC
BpmI           78  CTGGAG               CTGGAG(16/14)
Bpu1102I       18  GCTCAGC              GC^T[ACGT]AGC
BpuAI         499  GAAGAC               GAAGAC(2/6)
BsaHI         414  GGCGTC               G[GA]^CG[CT]C
BsgI           51  GTGCAG               GTGCAG(16/14)
BsmFI         510  GTCCC                GGGAC(10/14)
BsrBI          72  GAGCGG               CCGCTC(-3/-3)
BsrDI         792  CATTGC               GCAATG(2/0)
BsrFI         914  ACCGGC               [GA]^CCGG[CT]
BsrI          278  ACTGG                ACTGG(1/-1)
BstXI         821  CCATCCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        880  CCTGAGG              CC^T[ACGT]AGG
CelII          18  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        914  ACCGGC               [GA]^CCGG[CT]
DpnII         137  GATC                 ^GATC
DsaI          843  CCGCGG               C^C[GA][CT]GG
Eco81I        880  CCTGAGG              CC^T[ACGT]AGG
EcoNI          92  CCTATACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         931  GATATC               GAT^ATC
EcoT14I       896  CCAAGG               C^C[AT][AT]GG
HgaI          415  GCGTC                GACGC(5/10)
Hin1I         414  GGCGTC               G[GA]^CG[CT]C
KspI          843  CCGCGG               CCGC^GG
MaeII         553  ACGT                 A^CGT
MboI          137  GATC                 ^GATC
MfeI          533  CAATTG               C^AATTG
MunI          533  CAATTG               C^AATTG
NdeII         137  GATC                 ^GATC
PaeR7I        333  CTCGAG               C^TCGAG
PleI          373  GACTC                GAGTC(4/5)
PpuMI         142  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         588  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          679  CTGCAG               CTGCA^G
SacI           81  GAGCTC               GAGCT^C
SacII         843  CCGCGG               CCGC^GG
Sau3AI        137  GATC                 ^GATC
ScaI          157  AGTACT               AGT^ACT
SexAI         503  ACCTGGT              A^CC[AT]GGT
SfcI          679  CTGCAG               C^T[GA][CT]AG
SmlI          333  CTCGAG               C^T[CT][GA]AG
StuI          180  AGGCCT               AGG^CCT
StyI          896  CCAAGG               C^C[AT][AT]GG
TfiI          214  GATTC                G^A[AT]TC
XhoI          333  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         685  CGCG                 CG^CG
              844  CGCG                
AcsI           31  GAATTC               [GA]^AATT[CT]
              526  GAATTC              
AhdI          502  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              722  GACGGACTGTC         
ApoI           31  GAATTC               [GA]^AATT[CT]
              526  GAATTC              
AspEI         502  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              722  GACGGACTGTC         
BanI          282  GGCACC               G^G[CT][GA]CC
              856  GGCACC              
BseRI         307  CTCCTC               GAGGAG(10/8)
              430  GAGGAG              
BstUI         685  CGCG                 CG^CG
              844  CGCG                
CfoI          684  GCGC                 GCG^C
              796  GCGC                
DraII         142  AGGACCT              [GA]G^G[ACGT]CC[CT]
              806  AGGCCCT             
Eam1105I      502  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              722  GACGGACTGTC         
EcoO109I      142  AGGACCT              [GA]G^G[ACGT]CC[CT]
              806  AGGCCCT             
EcoRI          31  GAATTC               G^AATTC
              526  GAATTC              
FauI          447  CCCGC                CCCGC(4/6)
              390  GCGGG               
HhaI          684  GCGC                 GCG^C
              796  GCGC                
HinP1I        684  GCGC                 G^CGC
              796  GCGC                
HinfI         214  GATTC                G^A[ACGT]TC
              373  GACTC               
HphI          262  GGTGA                GGTGA(8/7)
              665  TCACC               
MslI          552  CACGTTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              822  CATCCAGGTG          
MspA1I        258  CAGCGG               C[AC]G^C[GT]G
              843  CCGCGG              
MvnI          685  CGCG                 CG^CG
              844  CGCG                
SmaI          313  CCCGGG               CCC^GGG
              357  CCCGGG              
XmaCI         313  CCCGGG               C^CCGGG
              357  CCCGGG              
XmaI          313  CCCGGG               C^CCGGG
              357  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccI        AciI        AfaI        AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BspMI       BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       TaqI        TseI        Tsp509I     
TspRI       TthHB8I     
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