Restriction map for pF1KB6445
 (Linear) (Six-base) MAPSORT of: KIBB6445.seq  Check: 7298  from: 1  to: 945



KIBB6445.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     282     564     945 
   Size:        282     282     381

  Fragments arranged by size: 

                 381     282     282
 
AccI GT'mk_AC

Cuts at:      0     302     650     902     945 
   Size:        302     348     252      43

  Fragments arranged by size: 

                 348     302     252      43
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     945 
   Size:        130     815
 
AflIII A'CryG_T

Cuts at:      0     551     945 
   Size:        551     394
 
AhdI GACnn_n'nnGTC

Cuts at:      0     507     727     945 
   Size:        507     220     218

  Fragments arranged by size: 

                 507     220     218
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      61     270     945 
   Size:         61     209     675

  Fragments arranged by size: 

                 675     209      61
 
ApaI G_GGCC'C

Cuts at:      0     448     945 
   Size:        448     497
 
ApoI r'AATT_y

Cuts at:      0      31     526     945 
   Size:         31     495     419

  Fragments arranged by size: 

                 495     419      31
 
AvaI C'yCGr_G

Cuts at:      0     313     333     357     669     945 
   Size:        313      20      24     312     276

  Fragments arranged by size: 

                 313     312     276      24      20
 
BanI G'GyrC_C

Cuts at:      0     282     856     945 
   Size:        282     574      89

  Fragments arranged by size: 

                 574     282      89
 
BanII G_rGCy'C

Cuts at:      0      85     437     448     761     889     896     945 
   Size:         85     352      11     313     128       7      49

  Fragments arranged by size: 

                 352     313     128      85      49      11       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     506     945 
   Size:        506     439
 
BbvCI CC'TCA_GC

Cuts at:      0     811     945 
   Size:        811     134
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     681     715     945 
   Size:        681      34     230

  Fragments arranged by size: 

                 681     230      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      15     945 
   Size:         15     930
 
BlpI GC'TnA_GC

Cuts at:      0      19     945 
   Size:         19     926
 
Bme1580I G_kGCm'C

Cuts at:      0     278     285     448     945 
   Size:        278       7     163     497

  Fragments arranged by size: 

                 497     278     163       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     288     945 
   Size:        288     657
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     945 
   Size:         99     846
 
Bpu10I CC'TnA_GC

Cuts at:      0     811     945 
   Size:        811     134
 
BsaHI Gr'CG_yC

Cuts at:      0     415     945 
   Size:        415     530
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     748     778     945 
   Size:        748      30     167

  Fragments arranged by size: 

                 748     167      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     445     945 
   Size:        298     147     500

  Fragments arranged by size: 

                 500     298     147
 
BseYI C'CCAG_C

Cuts at:      0     441     945 
   Size:        441     504
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     945 
   Size:         72     873
 
BsiHKAI G_wGCw'C

Cuts at:      0      85     631     707     945 
   Size:         85     546      76     238

  Fragments arranged by size: 

                 546     238      85      76
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     278     285     437     448     631     707     761 
   Size:         85     193       7     152      11     183      76      54

Cuts at:    761     889     896     945 
   Size:        128       7      49

  Fragments arranged by size: 

                 193     183     152     128      85      76      54      49
                  11       7       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     282     564     611     662     686     945 
   Size:        282     282      47      51      24     259

  Fragments arranged by size: 

                 282     282     259      51      47      24
 
BsrBI CCG'CTC

Cuts at:      0      74     945 
   Size:         74     871
 
BsrDI GCAATG_nn'

Cuts at:      0     791     945 
   Size:        791     154
 
BsrFI r'CCGG_y

Cuts at:      0     914     945 
   Size:        914      31
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     828     945 
   Size:        828     117
 
Bsu36I CC'TnA_GG

Cuts at:      0     881     945 
   Size:        881      64
 
BtgI C'CryG_G

Cuts at:      0     843     945 
   Size:        843     102
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     377     945 
   Size:        377     568
 
BtsI GCAGTG_nn'

Cuts at:      0     231     402     945 
   Size:        231     171     543

  Fragments arranged by size: 

                 543     231     171
 
EcoICRI GAG'CTC

Cuts at:      0      83     945 
   Size:         83     862
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      99     130     945 
   Size:         99      31     815

  Fragments arranged by size: 

                 815      99      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      96     945 
   Size:         96     849
 
EcoO109I rG'GnC_Cy

Cuts at:      0     143     807     945 
   Size:        143     664     138

  Fragments arranged by size: 

                 664     143     138
 
EcoRI G'AATT_C

Cuts at:      0      31     526     945 
   Size:         31     495     419

  Fragments arranged by size: 

                 495     419      31
 
EcoRV GAT'ATC

Cuts at:      0     933     945 
   Size:        933      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     493     525     713     745     945 
   Size:        493      32     188      32     200

  Fragments arranged by size: 

                 493     200     188      32      32
 
MfeI C'AATT_G

Cuts at:      0     533     945 
   Size:        533     412
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     659     945 
   Size:        659     286
 
MslI CAynn'nnrTG

Cuts at:      0     556     826     945 
   Size:        556     270     119

  Fragments arranged by size: 

                 556     270     119
 
MspA1I CmG'CkG

Cuts at:      0     260     845     945 
   Size:        260     585     100

  Fragments arranged by size: 

                 585     260     100
 
PasI CC'CwG_GG

Cuts at:      0     513     945 
   Size:        513     432
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     679     711     945 
   Size:        679      32     234

  Fragments arranged by size: 

                 679     234      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     143     945 
   Size:        143     802
 
PshAI GACnn'nnGTC

Cuts at:      0     592     945 
   Size:        592     353
 
PspOMI G'GGCC_C

Cuts at:      0     444     945 
   Size:        444     501
 
PstI C_TGCA'G

Cuts at:      0     683     945 
   Size:        683     262
 
SacI G_AGCT'C

Cuts at:      0      85     945 
   Size:         85     860
 
SacII CC_GC'GG

Cuts at:      0     846     945 
   Size:        846      99
 
ScaI AGT'ACT

Cuts at:      0     159     945 
   Size:        159     786
 
SexAI A'CCwGG_T

Cuts at:      0     503     945 
   Size:        503     442
 
SfcI C'TryA_G

Cuts at:      0     679     945 
   Size:        679     266
 
SmaI CCC'GGG

Cuts at:      0     315     359     945 
   Size:        315      44     586

  Fragments arranged by size: 

                 586     315      44
 
SmlI C'TyrA_G

Cuts at:      0     333     945 
   Size:        333     612
 
StuI AGG'CCT

Cuts at:      0     182     945 
   Size:        182     763
 
StyI C'CwwG_G

Cuts at:      0     896     945 
   Size:        896      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      43      77     286     945 
   Size:         43      34     209     659

  Fragments arranged by size: 

                 659     209      43      34
 
TatI w'GTAC_w

Cuts at:      0     157     833     945 
   Size:        157     676     112

  Fragments arranged by size: 

                 676     157     112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     748     780     849     881     945 
   Size:        748      32      69      32      64

  Fragments arranged by size: 

                 748      69      64      32      32
 
XhoI C'TCGA_G

Cuts at:      0     333     945 
   Size:        333     612
 
XmaI C'CCGG_G

Cuts at:      0     313     357     945 
   Size:        313      44     588

  Fragments arranged by size: 

                 588     313      44
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AlfI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI     BglI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BstXI
   Bsu36I     BtgI    BtgZI     BtsI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV    Hin4I     MfeI     MmeI     MslI   MspA1I     PasI
     PpiI    PpuMI    PshAI   PspOMI     PstI     SacI    SacII     ScaI
    SexAI     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XmnI
     ZraI
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