Restriction map for
pF1KB9552
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HinfI HpaI
KpnI Ksp632I MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 286 GGCGCC G[GA]^CG[CT]C
AlwNI 895 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 324 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1383 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 286 GGCGCC GGCGC^C
BbvCI 1146 CCTCAGC CCTCAGC(-5/-2)
BcgI 356 GCAGGAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 91 CTAG C^TAG
BpmI 423 CTGGAG CTGGAG(16/14)
BsaHI 286 GGCGCC G[GA]^CG[CT]C
BsgI 385 CTGCAC GTGCAG(16/14)
BsrBI 472 GAGCGG CCGCTC(-3/-3)
BssSI 847 CACGAG CACGAG(-5/-1)
DraIII 1040 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 286 GGCGCC G[GA]^CG[CT]C
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
KasI 286 GGCGCC G^GCGCC
KspI 1079 CCGCGG CCGC^GG
MaeI 91 CTAG C^TAG
MaeIII 443 GTGAC ^GT[ACGT]AC
MslI 267 CATGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 946 GCCGGC GCC^GGC
NarI 286 GGCGCC GG^CGCC
NgoMIV 946 GCCGGC G^CCGGC
PflFI 1383 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1355 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1224 AGGACCC [GA]G^G[AT]CC[CT]
PstI 745 CTGCAG CTGCA^G
SacI 397 GAGCTC GAGCT^C
SacII 1079 CCGCGG CCGC^GG
SexAI 1041 ACCTGGT A^CC[AT]GGT
SfcI 745 CTGCAG C^T[GA][CT]AG
SfoI 286 GGCGCC GGC^GCC
StuI 1250 AGGCCT AGG^CCT
Tsp45I 443 GTGAC ^GT[GC]AC
Tth111I 1383 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1355 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 324 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BalI 977 TGGCCA TGG^CCA
1236 TGGCCA
BbsI 735 GAAGAC GAAGAC(2/6)
1068 GAAGAC
BpuAI 735 GAAGAC GAAGAC(2/6)
1068 GAAGAC
BspMI 383 ACCTGC ACCTGC(4/8)
899 ACCTGC
BsrFI 641 ACCGGC [GA]^CCGG[CT]
946 GCCGGC
BssHII 80 GCGCGC G^CGCGC
352 GCGCGC
BstXI 699 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1248 CCAGGCCTCTGG
BstYI 15 GGATCT [GA]^GATC[CT]
998 GGATCT
Cfr10I 641 ACCGGC [GA]^CCGG[CT]
946 GCCGGC
EcoT14I 705 CCATGG C^C[AT][AT]GG
785 CCATGG
MaeII 308 ACGT A^CGT
824 ACGT
MflI 15 GGATCT [GA]^GATC[CT]
998 GGATCT
MluNI 977 TGGCCA TGG^CCA
1236 TGGCCA
MscI 977 TGGCCA TGG^CCA
1236 TGGCCA
NcoI 705 CCATGG C^CATGG
785 CCATGG
SfaNI 1051 GATGC GCATC(5/9)
212 GCATC
SfiI 1085 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1237 GGCCACTCGGGCC
SmaI 72 CCCGGG CCC^GGG
135 CCCGGG
StyI 705 CCATGG C^C[AT][AT]GG
785 CCATGG
TaqI 461 TCGA T^CGA
812 TCGA
TthHB8I 461 TCGA T^CGA
812 TCGA
XhoII 15 GGATCT [GA]^GATC[CT]
998 GGATCT
XmaCI 72 CCCGGG C^CCGGG
135 CCCGGG
XmaI 72 CCCGGG C^CCGGG
135 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BlpI BmyI Bpu1102I BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
TspRI