Restriction map for pF1KB9552
 (Circular) (Six-base) MAPSORT of: pF1KB9552.seq  Check: 6849  from: 1  to: 4511



pF1KB9552  4511 bp

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    500     500 
   Size:       4511
 
AccI GT'mk_AC

Cuts at:   1549    1549 
   Size:       4511
 
Acc65I G'GTAC_C

Cuts at:   1527    1527 
   Size:       4511
 
AclI AA'CG_TT

Cuts at:   4351    4351 
   Size:       4511
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2336    2768    3515    3783    2336 
   Size:        432     747     268    3064

  Fragments arranged by size: 

                3064     747     432     268
 
AflIII A'CryG_T

Cuts at:   1863    2967    3944    1863 
   Size:       1104     977    2430

  Fragments arranged by size: 

                2430    1104     977
 
AgeI A'CCGG_T

Cuts at:   2899    2899 
   Size:       4511
 
AlwNI CAG_nnn'CTG

Cuts at:   1008    1816    3383    1008 
   Size:        808    1567    2136

  Fragments arranged by size: 

                2136    1567     808
 
ApaI G_GGCC'C

Cuts at:    206    1196    1205    1397    1448     206 
   Size:        990       9     192      51    3269

  Fragments arranged by size: 

                3269     990     192      51       9
 
ApaLI G'TGCA_C

Cuts at:   3281    3281 
   Size:       4511
 
ApoI r'AATT_y

Cuts at:   1515    3864    4120    1515 
   Size:       2349     256    1906

  Fragments arranged by size: 

                2349    1906     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4511
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4511
 
AvaI C'yCGr_G

Cuts at:    180     243     790    1350    1531     180 
   Size:         63     547     560     181    3160

  Fragments arranged by size: 

                3160     560     547     181      63
 
BamHI G'GATC_C

Cuts at:   1536    1536 
   Size:       4511
 
BanI G'GyrC_C

Cuts at:    369     394     752    1022    1527    1941    2189    2224     369 
   Size:         25     358     270     505     414     248      35    2656

  Fragments arranged by size: 

                2656     505     414     358     270     248      35      25
            
 
BanII G_rGCy'C

Cuts at:    148     157     167     206     453     509     769    1196    1205 
   Size:          9      10      39     247      56     260     427       9

Cuts at:   1205    1397    1448    1525    2555     148 
   Size:        192      51      77    1030    2104

  Fragments arranged by size: 

                2104    1030     427     260     247     192      77      56
                  51      39      10       9       9
 
BbeI G_GCGC'C

Cuts at:    398    1945    2193     398 
   Size:       1547     248    2716

  Fragments arranged by size: 

                2716    1547     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    850    1183     850 
   Size:        333    4178
 
BbvCI CC'TCA_GC

Cuts at:   1255    1255 
   Size:       4511
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    453     487     453 
   Size:         34    4477
 
BciVI GTATCCnnnnn_n'

Cuts at:   2409    3170    4511    2409 
   Size:        761    1341    2409

  Fragments arranged by size: 

                2409    1341     761
 
BclI T'GATC_A

Cuts at:   2031    2031 
   Size:       4511
 
BglI GCCn_nnn'nGGC

Cuts at:    152     636     718    1020    1200    1352     152 
   Size:        484      82     302     180     152    3311

  Fragments arranged by size: 

                3311     484     302     180     152      82
 
BglII A'GATC_T

Cuts at:   2026    4063    2026 
   Size:       2037    2474
 
BlpI GC'TnA_GC

Cuts at:    142     346     503     508     578    1275    1623     142 
   Size:        204     157       5      70     697     348    3030

  Fragments arranged by size: 

                3030     697     348     204     157      70       5
 
Bme1580I G_kGCm'C

Cuts at:    206     374     679    1025    1077    1196    1205    1397    1448 
   Size:        168     305     346      52     119       9     192      51

Cuts at:   1448    2136    2229    3285     206 
   Size:        688      93    1056    1432

  Fragments arranged by size: 

                1432    1056     688     346     305     192     168     119
                  93      52      51       9
 
BmgBI CAC'GTC

Cuts at:    417     417 
   Size:       4511
 
BmrI ACTGGGnnnn_n'

Cuts at:   1296    1443    1479    1723    1804    2139    4283    1296 
   Size:        147      36     244      81     335    2144    1524

  Fragments arranged by size: 

                2144    1524     335     244     147      81      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    816    2878     816 
   Size:       2062    2449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    552     552 
   Size:       4511
 
Bpu10I CC'TnA_GC

Cuts at:    452    1255     452 
   Size:        803    3708
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1687    2566    3058    3356    1687 
   Size:        879     492     298    2842

  Fragments arranged by size: 

                2842     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    970    1146    1177    4215     970 
   Size:        176      31    3038    1266

  Fragments arranged by size: 

                3038    1266     176      31
 
BsaAI yAC'GTr

Cuts at:   1864    1864 
   Size:       4511
 
BsaBI GATnn'nnATC

Cuts at:      6    2025    4062       6 
   Size:       2019    2037     455

  Fragments arranged by size: 

                2037    2019     455
 
BsaHI Gr'CG_yC

Cuts at:    395    1942    2190    2892    4388     395 
   Size:       1547     248     702    1496     518

  Fragments arranged by size: 

                1547    1496     702     518     248
 
BsaWI w'CCGG_w

Cuts at:   1700    2221    2899    3173    3320    3731    1700 
   Size:        521     678     274     147     411    2480

  Fragments arranged by size: 

                2480     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    874     904     874 
   Size:         30    4481
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    130     175     586     130 
   Size:         45     411    4055

  Fragments arranged by size: 

                4055     411      45
 
BseYI C'CCAG_C

Cuts at:    258     519    1065    1432    1936    2694    3271    3767     258 
   Size:        261     546     367     504     758     577     496    1002

  Fragments arranged by size: 

                1002     758     577     546     504     496     367     261
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    478     478 
   Size:       4511
 
BsiEI CG_ry'CG

Cuts at:    104    2053    2099    3307     104 
   Size:       1949      46    1208    1308

  Fragments arranged by size: 

                1949    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    347     509     797    1525    2303    2493    3285     347 
   Size:        162     288     728     778     190     792    1573

  Fragments arranged by size: 

                1573     792     778     728     288     190     162
 
Bsp1286I G_dGCh'C

Cuts at:    148     157     167     206     347     374     453     509     679 
   Size:          9      10      39     141      27      79      56     170

Cuts at:    679     769     797    1025    1077    1196    1205    1397    1448 
   Size:         90      28     228      52     119       9     192      51

Cuts at:   1448    1525    2136    2229    2303    2493    2555    3285     148 
   Size:         77     611      93      74     190      62     730    1374

  Fragments arranged by size: 

                1374     730     611     228     192     190     170     141
                 119      93      90      79      77      74      62      56
                  52      51      39      28      27      10       9       9
            
 
BspEI T'CCGG_A

Cuts at:   3731    3731 
   Size:       4511
 
BspHI T'CATG_A

Cuts at:   3687    3687 
   Size:       4511
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    500    1016    1561    1775    2077    2458     500 
   Size:        516     545     214     302     381    2553

  Fragments arranged by size: 

                2553     545     516     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     582    2803    2857    4341    4506      50 
   Size:        532    2221      54    1484     165      55

  Fragments arranged by size: 

                2221    1484     532     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2423    2423 
   Size:       4511
 
BsrFI r'CCGG_y

Cuts at:    749    1054    2509    2690    2899     749 
   Size:        305    1455     181     209    2361

  Fragments arranged by size: 

                2361    1455     305     209     181
 
BssHII G'CGCG_C

Cuts at:    188     460     188 
   Size:        272    4239
 
BssSI C'ACGA_G

Cuts at:    955    2782    3140     955 
   Size:       1827     358    2326

  Fragments arranged by size: 

                2326    1827     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1888    1888 
   Size:       4511
 
BstBI TT'CG_AA

Cuts at:   2872    2872 
   Size:       4511
 
BstXI CCAn_nnnn'nTGG

Cuts at:    814    1363     814 
   Size:        549    3962
 
BstYI r'GATC_y

Cuts at:      1     123    1106    1536    2003    2026    2361    2607    3619 
   Size:        122     983     430     467      23     335     246    1012

Cuts at:   3619    3705    3717    4063       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     983     467     449     430     346     335     246
                 122      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    801    1329    1440     801 
   Size:        528     111    3872

  Fragments arranged by size: 

                3872     528     111
 
BtgI C'CryG_G

Cuts at:    107     149     813     893    1082    1136    1187     107 
   Size:         42     664      80     189      54      51    3431

  Fragments arranged by size: 

                3431     664     189      80      54      51      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2461    2642    2805    2461 
   Size:        181     163    4167

  Fragments arranged by size: 

                4167     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1077    1754    1077 
   Size:        677    3834
 
DraI TTT'AAA

Cuts at:   1510    1510 
   Size:       4511
 
DraIII CAC_nnn'GTG

Cuts at:   1153    1153 
   Size:       4511
 
DrdI GACnn_nn'nnGTC

Cuts at:   2217    3075    2217 
   Size:        858    3653
 
EaeI y'GGCC_r

Cuts at:   1079    1085    1344    1418    2096    2270    2661    2688    4033 
   Size:          6     259      74     678     174     391      27    1345

Cuts at:   4033    1079 
   Size:       1557

  Fragments arranged by size: 

                1557    1345     678     391     259     174      74      27
                   6
 
EagI C'GGCC_G

Cuts at:   2096    2096 
   Size:       4511
 
EarI CTCTTCn'nnn_

Cuts at:   2534    2744    2534 
   Size:        210    4301
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    127     236     310     654     685    1089    2719    3029    3175 
   Size:        109      74     344      31     404    1630     310     146

Cuts at:   3175    4321     127 
   Size:       1146     317

  Fragments arranged by size: 

                1630    1146     404     344     317     310     146     109
                  74      31
 
EcoICRI GAG'CTC

Cuts at:    507    1523     507 
   Size:       1016    3495
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    552    2336    2768    3515    3783     552 
   Size:       1784     432     747     268    1280

  Fragments arranged by size: 

                1784    1280     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    202    1192    1193    1201    1202    1333    1393    1394    1444 
   Size:        990       1       8       1     131      60       1      50

Cuts at:   1444    1445    1650     202 
   Size:          1     205    3063

  Fragments arranged by size: 

                3063     990     205     131      60      50       8       1
                   1       1       1
 
EcoRI G'AATT_C

Cuts at:   1515    1515 
   Size:       4511
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    579    2530     579 
   Size:       1951    2560
 
FspI TGC'GCA

Cuts at:   2292    2292 
   Size:       4511
 
HaeII r_GCGC'y

Cuts at:    398     561     737     891    1945    2193    3215    4194     398 
   Size:        163     176     154    1054     248    1022     979     715

  Fragments arranged by size: 

                1054    1022     979     715     248     176     163     154
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    841     873     874     906     949     981    1210    1242    2353 
   Size:         32       1      32      43      32     229      32    1111

Cuts at:   2353    2383    2385    2415     841 
   Size:         30       2      30    2937

  Fragments arranged by size: 

                2937    1111     229      43      32      32      32      32
                  30      30       2       1
 
HincII GTy'rAC

Cuts at:    390    1550     390 
   Size:       1160    3351
 
KasI G'GCGC_C

Cuts at:    394    1941    2189     394 
   Size:       1547     248    2716

  Fragments arranged by size: 

                2716    1547     248
 
KpnI G_GTAC'C

Cuts at:   1531    1531 
   Size:       4511
 
MluI A'CGCG_T

Cuts at:   3944    3944 
   Size:       4511
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    856     977     992    3182    3366     856 
   Size:        121      15    2190     184    2001

  Fragments arranged by size: 

                2190    2001     184     121      15
 
MscI TGG'CCA

Cuts at:   1087    1346    2272    1087 
   Size:        259     926    3326

  Fragments arranged by size: 

                3326     926     259
 
MslI CAynn'nnrTG

Cuts at:    379    2500    2627     379 
   Size:       2121     127    2263

  Fragments arranged by size: 

                2263    2121     127
 
MspA1I CmG'CkG

Cuts at:    170     350    1189    1404    1622    1936    2296    3309    3554 
   Size:        180     839     215     218     314     360    1013     245

Cuts at:   3554     170 
   Size:       1127

  Fragments arranged by size: 

                1127    1013     839     360     314     245     218     215
                 180
 
NaeI GCC'GGC

Cuts at:   1056    2692    1056 
   Size:       1636    2875
 
NarI GG'CG_CC

Cuts at:    395    1942    2190     395 
   Size:       1547     248    2716

  Fragments arranged by size: 

                2716    1547     248
 
NcoI C'CATG_G

Cuts at:    107     813     893     107 
   Size:        706      80    3725

  Fragments arranged by size: 

                3725     706      80
 
NgoMIV G'CCGG_C

Cuts at:   1054    2690    1054 
   Size:       1636    2875
 
NspI r_CATG'y

Cuts at:   1564    2064    2971    1564 
   Size:        500     907    3104

  Fragments arranged by size: 

                3104     907     500
 
PasI CC'CwG_GG

Cuts at:    660    1197     660 
   Size:        537    3974
 
PciI A'CATG_T

Cuts at:   2967    2967 
   Size:       4511
 
PflMI CCAn_nnn'nTGG

Cuts at:   1469    1469 
   Size:       4511
 
PmeI GTTT'AAAC

Cuts at:   1510    1510 
   Size:       4511
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3650    3682    3650 
   Size:         32    4479
 
PpuMI rG'GwC_Cy

Cuts at:   1333    1333 
   Size:       4511
 
PspOMI G'GGCC_C

Cuts at:    202    1192    1201    1393    1444     202 
   Size:        990       9     192      51    3269

  Fragments arranged by size: 

                3269     990     192      51       9
 
PstI C_TGCA'G

Cuts at:    857    1558    2243     857 
   Size:        701     685    3125

  Fragments arranged by size: 

                3125     701     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4511
 
PvuII CAG'CTG

Cuts at:   1936    2296    1936 
   Size:        360    4151
 
RsrII CG'GwC_CG

Cuts at:   2706    2706 
   Size:       4511
 
SacI G_AGCT'C

Cuts at:    509    1525     509 
   Size:       1016    3495
 
SacII CC_GC'GG

Cuts at:   1190    1190 
   Size:       4511
 
SalI G'TCGA_C

Cuts at:   1548    1548 
   Size:       4511
 
SapI GCTCTTCn'nnn_

Cuts at:   2534    2744    2534 
   Size:        210    4301
 
SbfI CC_TGCA'GG

Cuts at:   1558    1558 
   Size:       4511
 
ScaI AGT'ACT

Cuts at:   4028    4028 
   Size:       4511
 
SexAI A'CCwGG_T

Cuts at:   1149    1149 
   Size:       4511
 
SfcI C'TryA_G

Cuts at:     33     853    1554    2239    3232    3423      33 
   Size:        820     701     685     993     191    1121

  Fragments arranged by size: 

                1121     993     820     701     685     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1200    1352    1200 
   Size:        152    4359
 
SfoI GGC'GCC

Cuts at:    396    1943    2191     396 
   Size:       1547     248    2716

  Fragments arranged by size: 

                2716    1547     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4511
 
SmaI CCC'GGG

Cuts at:    182     245    1533     182 
   Size:         63    1288    3160

  Fragments arranged by size: 

                3160    1288      63
 
SmlI C'TyrA_G

Cuts at:   1666    2581    3073    3335    1666 
   Size:        915     492     262    2842

  Fragments arranged by size: 

                2842     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4511
 
SphI G_CATG'C

Cuts at:   1564    2064    1564 
   Size:        500    4011
 
StuI AGG'CCT

Cuts at:   1360    1360 
   Size:       4511
 
StyI C'CwwG_G

Cuts at:    107     813     893    1645    1999     107 
   Size:        706      80     752     354    2619

  Fragments arranged by size: 

                2619     752     706     354      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    770     837    1040    2094    2227    2686    2895     770 
   Size:         67     203    1054     133     459     209    2386

  Fragments arranged by size: 

                2386    1054     459     209     203     133      67
 
TatI w'GTAC_w

Cuts at:   4026    4026 
   Size:       4511
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4028    4060    4028 
   Size:         32    4479
 
Tth111I GACn'n_nGTC

Cuts at:   1494    2308    1494 
   Size:        814    3697
 
XbaI T'CTAG_A

Cuts at:   1542    1542 
   Size:       4511
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2006    2006 
   Size:       4511
 
XmaI C'CCGG_G

Cuts at:    180     243    1531     180 
   Size:         63    1288    3160

  Fragments arranged by size: 

                3160    1288      63
 
XmnI GAAnn'nnTTC

Cuts at:    436     436 
   Size:       4511
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI    BfrBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI
   BstEII  BstZ17I     ClaI    CspCI    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmlI    PshAI     PsiI    PspXI     PsrI    SanDI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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