Restriction map for pF1KB7823
 (Linear) (Six-base) MAPSORT of: KIBB7823.seq  Check: 6238  from: 1  to: 1608



KIBB7823.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1258    1608 
   Size:       1258     350
 
AccI GT'mk_AC

Cuts at:      0    1449    1608 
   Size:       1449     159
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     296    1123    1608 
   Size:         80     216     827     485

  Fragments arranged by size: 

                 827     485     216      80
 
AflIII A'CryG_T

Cuts at:      0    1370    1608 
   Size:       1370     238
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1120    1608 
   Size:       1120     488
 
ApoI r'AATT_y

Cuts at:      0     650     846    1283    1608 
   Size:        650     196     437     325

  Fragments arranged by size: 

                 650     437     325     196
 
AseI AT'TA_AT

Cuts at:      0     641    1608 
   Size:        641     967
 
AvaI C'yCGr_G

Cuts at:      0     656    1608 
   Size:        656     952
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     142     175    1608 
   Size:        142      33    1433

  Fragments arranged by size: 

                1433     142      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     127     143     224    1608 
   Size:        127      16      81    1384

  Fragments arranged by size: 

                1384     127      81      16
 
BclI T'GATC_A

Cuts at:      0    1299    1608 
   Size:       1299     309
 
BfrBI ATG'CAT

Cuts at:      0     690    1608 
   Size:        690     918
 
BglI GCCn_nnn'nGGC

Cuts at:      0     859    1608 
   Size:        859     749
 
Bme1580I G_kGCm'C

Cuts at:      0     918    1608 
   Size:        918     690
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     204    1343    1608 
   Size:        204    1139     265

  Fragments arranged by size: 

                1139     265     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590    1329    1608 
   Size:        590     739     279

  Fragments arranged by size: 

                 739     590     279
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73    1608 
   Size:         73    1535
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     108    1608 
   Size:        108    1500
 
BsaAI yAC'GTr

Cuts at:      0    1048    1608 
   Size:       1048     560
 
BsaWI w'CCGG_w

Cuts at:      0     137    1097    1608 
   Size:        137     960     511

  Fragments arranged by size: 

                 960     511     137
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1302    1332    1608 
   Size:       1302      30     276

  Fragments arranged by size: 

                1302     276      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      96     662    1608 
   Size:         96     566     946

  Fragments arranged by size: 

                 946     566      96
 
BseYI C'CCAG_C

Cuts at:      0    1006    1608 
   Size:       1006     602
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       8    1608 
   Size:          8    1600
 
BsiWI C'GTAC_G

Cuts at:      0     128    1608 
   Size:        128    1480
 
BsmI GAATG_Cn'

Cuts at:      0     255     381     559     690    1608 
   Size:        255     126     178     131     918

  Fragments arranged by size: 

                 918     255     178     131     126
 
Bsp1286I G_dGCh'C

Cuts at:      0     918    1608 
   Size:        918     690
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      30     401    1258    1608 
   Size:         30     371     857     350

  Fragments arranged by size: 

                 857     371     350      30
 
BsrDI GCAATG_nn'

Cuts at:      0     875    1608 
   Size:        875     733
 
BssSI C'ACGA_G

Cuts at:      0     893    1576    1608 
   Size:        893     683      32

  Fragments arranged by size: 

                 893     683      32
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267    1251    1608 
   Size:        267     984     357

  Fragments arranged by size: 

                 984     357     267
 
BstYI r'GATC_y

Cuts at:      0      73     197     612    1488    1608 
   Size:         73     124     415     876     120

  Fragments arranged by size: 

                 876     415     124     120      73
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     712     747    1608 
   Size:        712      35     861

  Fragments arranged by size: 

                 861     712      35
 
EaeI y'GGCC_r

Cuts at:      0     257    1608 
   Size:        257    1351
 
EarI CTCTTCn'nnn_

Cuts at:      0     108     810     956     996    1237    1588    1608 
   Size:        108     702     146      40     241     351      20

  Fragments arranged by size: 

                 702     351     241     146     108      40      20
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     296     590    1123    1329    1608 
   Size:         80     216     294     533     206     279

  Fragments arranged by size: 

                 533     294     279     216     206      80
 
EcoRV GAT'ATC

Cuts at:      0    1161    1608 
   Size:       1161     447
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     985    1608 
   Size:        985     623
 
HaeII r_GCGC'y

Cuts at:      0       7    1608 
   Size:          7    1601
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     995    1002    1027    1034    1114    1146    1608 
   Size:        995       7      25       7      80      32     462

  Fragments arranged by size: 

                 995     462      80      32      25       7       7
 
MfeI C'AATT_G

Cuts at:      0     299    1608 
   Size:        299    1309
 
MscI TGG'CCA

Cuts at:      0     259    1608 
   Size:        259    1349
 
MslI CAynn'nnrTG

Cuts at:      0     265     839    1580    1608 
   Size:        265     574     741      28

  Fragments arranged by size: 

                 741     574     265      28
 
MspA1I CmG'CkG

Cuts at:      0     502    1608 
   Size:        502    1106
 
NsiI A_TGCA'T

Cuts at:      0     692    1608 
   Size:        692     916
 
NspI r_CATG'y

Cuts at:      0    1374    1608 
   Size:       1374     234
 
PciI A'CATG_T

Cuts at:      0    1370    1608 
   Size:       1370     238
 
PmlI CAC'GTG

Cuts at:      0    1048    1608 
   Size:       1048     560
 
PshAI GACnn'nnGTC

Cuts at:      0     797    1608 
   Size:        797     811
 
PsiI TTA'TAA

Cuts at:      0    1289    1608 
   Size:       1289     319
 
PvuII CAG'CTG

Cuts at:      0     502    1608 
   Size:        502    1106
 
SapI GCTCTTCn'nnn_

Cuts at:      0     996    1237    1608 
   Size:        996     241     371

  Fragments arranged by size: 

                 996     371     241
 
SexAI A'CCwGG_T

Cuts at:      0     353    1608 
   Size:        353    1255
 
SfcI C'TryA_G

Cuts at:      0     823    1608 
   Size:        823     785
 
SmaI CCC'GGG

Cuts at:      0     658    1608 
   Size:        658     950
 
SmlI C'TyrA_G

Cuts at:      0      52    1608 
   Size:         52    1556
 
StyI C'CwwG_G

Cuts at:      0     628    1244    1568    1608 
   Size:        628     616     324      40

  Fragments arranged by size: 

                 628     616     324      40
 
TatI w'GTAC_w

Cuts at:      0     831    1608 
   Size:        831     777
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     879     911    1608 
   Size:        879      32     697

  Fragments arranged by size: 

                 879     697      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     328    1608 
   Size:        328    1280
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1356    1608 
   Size:       1356     252
 
XmaI C'CCGG_G

Cuts at:      0     656    1608 
   Size:        656     952
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI
     BaeI    BciVI     BclI    BfrBI     BglI Bme1580I     BmrI     BpmI
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiWI     BsmI Bsp1286I    BspMI    BsrDI    BssSI    BstXI    BstYI
    CspCI     EaeI     EarI  Eco57MI    EcoRV     FalI    HaeII    Hin4I
     MfeI     MscI     MslI   MspA1I     NsiI     NspI     PciI     PmlI
    PshAI     PsiI    PvuII     SapI    SexAI     SfcI     SmaI     SmlI
     StyI     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BsaBI    BsaHI    BsiEI  BsiHKAI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     XbaI     XhoI     XmnI     ZraI
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