Restriction map for pF1KB6705
 (Circular) (Six-base) MAPSORT of: pF1KB6705.seq  Check: 8037  from: 1  to: 3341



pF1KB6705  3341 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:    379     379 
   Size:       3341
 
Acc65I G'GTAC_C

Cuts at:    357     357 
   Size:       3341
 
AclI AA'CG_TT

Cuts at:   3181    3181 
   Size:       3341
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1166    1598    2345    2613    1166 
   Size:        432     747     268    1894

  Fragments arranged by size: 

                1894     747     432     268
 
AflIII A'CryG_T

Cuts at:    183     693    1797    2774     183 
   Size:        510    1104     977     750

  Fragments arranged by size: 

                1104     977     750     510
 
AgeI A'CCGG_T

Cuts at:   1729    1729 
   Size:       3341
 
AlwNI CAG_nnn'CTG

Cuts at:    646    2213     646 
   Size:       1567    1774
 
ApaLI G'TGCA_C

Cuts at:   2111    2111 
   Size:       3341
 
ApoI r'AATT_y

Cuts at:    139     345    2694    2950     139 
   Size:        206    2349     256     530

  Fragments arranged by size: 

                2349     530     256     206
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3341
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3341
 
AvaI C'yCGr_G

Cuts at:    127     361     127 
   Size:        234    3107
 
BamHI G'GATC_C

Cuts at:    366     366 
   Size:       3341
 
BanI G'GyrC_C

Cuts at:    357     771    1019    1054     357 
   Size:        414     248      35    2644

  Fragments arranged by size: 

                2644     414     248      35
 
BanII G_rGCy'C

Cuts at:    355    1385     355 
   Size:       1030    2311
 
BbeI G_GCGC'C

Cuts at:    775    1023     775 
   Size:        248    3093
 
BciVI GTATCCnnnnn_n'

Cuts at:   1239    2000    3341    1239 
   Size:        761    1341    1239

  Fragments arranged by size: 

                1341    1239     761
 
BclI T'GATC_A

Cuts at:    861     861 
   Size:       3341
 
BglII A'GATC_T

Cuts at:    856    2893     856 
   Size:       2037    1304
 
BlpI GC'TnA_GC

Cuts at:    453     453 
   Size:       3341
 
Bme1580I G_kGCm'C

Cuts at:    966    1059    2115     966 
   Size:         93    1056    2192

  Fragments arranged by size: 

                2192    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    553     634     969    3113     553 
   Size:         81     335    2144     781

  Fragments arranged by size: 

                2144     781     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    134    1708     134 
   Size:       1574    1767
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    517    1396    1888    2186     517 
   Size:        879     492     298    1672

  Fragments arranged by size: 

                1672     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3045    3045 
   Size:       3341
 
BsaAI yAC'GTr

Cuts at:    694     694 
   Size:       3341
 
BsaBI GATnn'nnATC

Cuts at:      6     855    2892       6 
   Size:        849    2037     455

  Fragments arranged by size: 

                2037     849     455
 
BsaHI Gr'CG_yC

Cuts at:    772    1020    1722    3218     772 
   Size:        248     702    1496     895

  Fragments arranged by size: 

                1496     895     702     248
 
BsaWI w'CCGG_w

Cuts at:    530    1051    1729    2003    2150    2561     530 
   Size:        521     678     274     147     411    1310

  Fragments arranged by size: 

                1310     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    766    1524    2101    2597     766 
   Size:        758     577     496    1510

  Fragments arranged by size: 

                1510     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     883     929    2137     104 
   Size:        779      46    1208    1308

  Fragments arranged by size: 

                1308    1208     779      46
 
BsiHKAI G_wGCw'C

Cuts at:    355    1133    1323    2115     355 
   Size:        778     190     792    1581

  Fragments arranged by size: 

                1581     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    355     966    1059    1133    1323    1385    2115     355 
   Size:        611      93      74     190      62     730    1581

  Fragments arranged by size: 

                1581     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2561    2561 
   Size:       3341
 
BspHI T'CATG_A

Cuts at:   2517    2517 
   Size:       3341
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    391     605     907    1288     391 
   Size:        214     302     381    2444

  Fragments arranged by size: 

                2444     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1633    1687    3171    3336      50 
   Size:       1583      54    1484     165      55

  Fragments arranged by size: 

                1583    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1253    1253 
   Size:       3341
 
BsrFI r'CCGG_y

Cuts at:   1339    1520    1729    1339 
   Size:        181     209    2951

  Fragments arranged by size: 

                2951     209     181
 
BssSI C'ACGA_G

Cuts at:   1612    1970    1612 
   Size:        358    2983
 
BstAPI GCAn_nnn'nTGC

Cuts at:    718     718 
   Size:       3341
 
BstBI TT'CG_AA

Cuts at:   1702    1702 
   Size:       3341
 
BstYI r'GATC_y

Cuts at:      1     366     833     856    1191    1437    2449    2535    2547 
   Size:        365     467      23     335     246    1012      86      12

Cuts at:   2547    2893       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     365     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1291    1472    1635    1291 
   Size:        181     163    2997

  Fragments arranged by size: 

                2997     181     163
 
BtsI GCAGTG_nn'

Cuts at:    584     584 
   Size:       3341
 
DraI TTT'AAA

Cuts at:    340     340 
   Size:       3341
 
DrdI GACnn_nn'nnGTC

Cuts at:   1047    1905    1047 
   Size:        858    2483
 
EaeI y'GGCC_r

Cuts at:    926    1100    1491    1518    2863     926 
   Size:        174     391      27    1345    1404

  Fragments arranged by size: 

                1404    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    926     926 
   Size:       3341
 
EarI CTCTTCn'nnn_

Cuts at:   1364    1574    1364 
   Size:        210    3131
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1549    1859    2005    3151    1549 
   Size:        310     146    1146    1739

  Fragments arranged by size: 

                1739    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    353     353 
   Size:       3341
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1166    1598    2345    2613    1166 
   Size:        432     747     268    1894

  Fragments arranged by size: 

                1894     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    480     480 
   Size:       3341
 
EcoRI G'AATT_C

Cuts at:    345     345 
   Size:       3341
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1360    1360 
   Size:       3341
 
FspI TGC'GCA

Cuts at:   1122    1122 
   Size:       3341
 
HaeII r_GCGC'y

Cuts at:    775    1023    2045    3024     775 
   Size:        248    1022     979    1092

  Fragments arranged by size: 

                1092    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1183    1213    1215    1245    1183 
   Size:         30       2      30    3279

  Fragments arranged by size: 

                3279      30      30       2
 
HincII GTy'rAC

Cuts at:    380     380 
   Size:       3341
 
KasI G'GCGC_C

Cuts at:    771    1019     771 
   Size:        248    3093
 
KpnI G_GTAC'C

Cuts at:    361     361 
   Size:       3341
 
MluI A'CGCG_T

Cuts at:   2774    2774 
   Size:       3341
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2012    2196    2012 
   Size:        184    3157
 
MscI TGG'CCA

Cuts at:   1102    1102 
   Size:       3341
 
MslI CAynn'nnrTG

Cuts at:   1330    1457    1330 
   Size:        127    3214
 
MspA1I CmG'CkG

Cuts at:    452     766    1126    2139    2384     452 
   Size:        314     360    1013     245    1409

  Fragments arranged by size: 

                1409    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1522    1522 
   Size:       3341
 
NarI GG'CG_CC

Cuts at:    772    1020     772 
   Size:        248    3093
 
NgoMIV G'CCGG_C

Cuts at:   1520    1520 
   Size:       3341
 
NspI r_CATG'y

Cuts at:    187     394     894    1801     187 
   Size:        207     500     907    1727

  Fragments arranged by size: 

                1727     907     500     207
 
PciI A'CATG_T

Cuts at:    183    1797     183 
   Size:       1614    1727
 
PmeI GTTT'AAAC

Cuts at:    340     340 
   Size:       3341
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2480    2512    2480 
   Size:         32    3309
 
PstI C_TGCA'G

Cuts at:    388    1073     388 
   Size:        685    2656
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3341
 
PvuII CAG'CTG

Cuts at:    766    1126     766 
   Size:        360    2981
 
RsrII CG'GwC_CG

Cuts at:   1536    1536 
   Size:       3341
 
SacI G_AGCT'C

Cuts at:    355     355 
   Size:       3341
 
SalI G'TCGA_C

Cuts at:    378     378 
   Size:       3341
 
SapI GCTCTTCn'nnn_

Cuts at:   1364    1574    1364 
   Size:        210    3131
 
SbfI CC_TGCA'GG

Cuts at:    388     388 
   Size:       3341
 
ScaI AGT'ACT

Cuts at:   2858    2858 
   Size:       3341
 
SfcI C'TryA_G

Cuts at:     33     384    1069    2062    2253      33 
   Size:        351     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     351     191
 
SfoI GGC'GCC

Cuts at:    773    1021     773 
   Size:        248    3093
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3341
 
SmaI CCC'GGG

Cuts at:    363     363 
   Size:       3341
 
SmlI C'TyrA_G

Cuts at:    496    1411    1903    2165     496 
   Size:        915     492     262    1672

  Fragments arranged by size: 

                1672     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     256      67 
   Size:        189    3152
 
SphI G_CATG'C

Cuts at:    394     894     394 
   Size:        500    2841
 
SspI AAT'ATT

Cuts at:    197     276     197 
   Size:         79    3262
 
StyI C'CwwG_G

Cuts at:    189     323     475     829     189 
   Size:        134     152     354    2701

  Fragments arranged by size: 

                2701     354     152     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    924    1057    1516    1725     924 
   Size:        133     459     209    2540

  Fragments arranged by size: 

                2540     459     209     133
 
TatI w'GTAC_w

Cuts at:   2856    2856 
   Size:       3341
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2858    2890    2858 
   Size:         32    3309
 
Tth111I GACn'n_nGTC

Cuts at:   1138    1138 
   Size:       3341
 
XbaI T'CTAG_A

Cuts at:    372     372 
   Size:       3341
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    836     836 
   Size:       3341
 
XmaI C'CCGG_G

Cuts at:    361     361 
   Size:       3341
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com