Restriction map for pF1KB6320
 (Linear) (Six-base) MAPSORT of: KIBB6320.seq  Check: 9097  from: 1  to: 846



KIBB6320.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

Acc65I G'GTAC_C

Cuts at:      0     717     846 
   Size:        717     129
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     846 
   Size:        166     680
 
AleI CACnn'nnGTG

Cuts at:      0     301     394     846 
   Size:        301      93     452

  Fragments arranged by size: 

                 452     301      93
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     846 
   Size:        120     726
 
AvaI C'yCGr_G

Cuts at:      0      10     423     721     846 
   Size:         10     413     298     125

  Fragments arranged by size: 

                 413     298     125      10
 
BanI G'GyrC_C

Cuts at:      0     295     447     717     846 
   Size:        295     152     270     129

  Fragments arranged by size: 

                 295     270     152     129
 
BanII G_rGCy'C

Cuts at:      0     665     846 
   Size:        665     181
 
BbeI G_GCGC'C

Cuts at:      0     451     846 
   Size:        451     395
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     259     846 
   Size:        259     587
 
BglII A'GATC_T

Cuts at:      0     827     846 
   Size:        827      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      99     846 
   Size:         99     747
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       7     343     646     846 
   Size:          7     336     303     200

  Fragments arranged by size: 

                 336     303     200       7
 
BsaHI Gr'CG_yC

Cuts at:      0     448     846 
   Size:        448     398
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     664     846 
   Size:        664     182
 
BseYI C'CCAG_C

Cuts at:      0     593     846 
   Size:        593     253
 
BsiHKAI G_wGCw'C

Cuts at:      0     665     846 
   Size:        665     181
 
Bsp1286I G_dGCh'C

Cuts at:      0     665     846 
   Size:        665     181
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     650     846 
   Size:        650     196
 
BsrBI CCG'CTC

Cuts at:      0     309     846 
   Size:        309     537
 
BsrDI GCAATG_nn'

Cuts at:      0     278     846 
   Size:        278     568
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     468     570     846 
   Size:        468     102     276

  Fragments arranged by size: 

                 468     276     102
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     846 
   Size:        396     450
 
BstYI r'GATC_y

Cuts at:      0     116     536     827     846 
   Size:        116     420     291      19

  Fragments arranged by size: 

                 420     291     116      19
 
BtgI C'CryG_G

Cuts at:      0     395     846 
   Size:        395     451
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     720     797     846 
   Size:        720      77      49

  Fragments arranged by size: 

                 720      77      49
 
EaeI y'GGCC_r

Cuts at:      0     111     356     531     846 
   Size:        111     245     175     315

  Fragments arranged by size: 

                 315     245     175     111
 
EarI CTCTTCn'nnn_

Cuts at:      0     322     846 
   Size:        322     524
 
EcoICRI GAG'CTC

Cuts at:      0     663     846 
   Size:        663     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     846 
   Size:        166     680
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     646     846 
   Size:        646     200
 
EcoO109I rG'GnC_Cy

Cuts at:      0     415     846 
   Size:        415     431
 
EcoRV GAT'ATC

Cuts at:      0     126     276     846 
   Size:        126     150     570

  Fragments arranged by size: 

                 570     150     126
 
HaeII r_GCGC'y

Cuts at:      0     194     451     846 
   Size:        194     257     395

  Fragments arranged by size: 

                 395     257     194
 
KasI G'GCGC_C

Cuts at:      0     447     846 
   Size:        447     399
 
KpnI G_GTAC'C

Cuts at:      0     721     846 
   Size:        721     125
 
MscI TGG'CCA

Cuts at:      0     113     533     846 
   Size:        113     420     313

  Fragments arranged by size: 

                 420     313     113
 
MslI CAynn'nnrTG

Cuts at:      0     301     394     846 
   Size:        301      93     452

  Fragments arranged by size: 

                 452     301      93
 
NarI GG'CG_CC

Cuts at:      0     448     846 
   Size:        448     398
 
NruI TCG'CGA

Cuts at:      0     782     846 
   Size:        782      64
 
NspI r_CATG'y

Cuts at:      0     432     471     492     846 
   Size:        432      39      21     354

  Fragments arranged by size: 

                 432     354      39      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     846 
   Size:        120     726
 
PfoI T'CCnGG_A

Cuts at:      0     119     846 
   Size:        119     727
 
PstI C_TGCA'G

Cuts at:      0     794     846 
   Size:        794      52
 
SacI G_AGCT'C

Cuts at:      0     665     846 
   Size:        665     181
 
SbfI CC_TGCA'GG

Cuts at:      0     794     846 
   Size:        794      52
 
SfcI C'TryA_G

Cuts at:      0     790     846 
   Size:        790      56
 
SfoI GGC'GCC

Cuts at:      0     449     846 
   Size:        449     397
 
SmaI CCC'GGG

Cuts at:      0     425     723     846 
   Size:        425     298     123

  Fragments arranged by size: 

                 425     298     123
 
SphI G_CATG'C

Cuts at:      0     432     846 
   Size:        432     414
 
StuI AGG'CCT

Cuts at:      0     163     521     846 
   Size:        163     358     325

  Fragments arranged by size: 

                 358     325     163
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     280     846 
   Size:        280     566
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     396     846 
   Size:        396     450
 
XmaI C'CCGG_G

Cuts at:      0     423     721     846 
   Size:        423     298     125

  Fragments arranged by size: 

                 423     298     125
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI    AlwNI     AvaI     BanI    BanII     BbeI
    BciVI    BglII     BmrI     BsaI    BsaHI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
    HaeII     KasI     KpnI     MscI     MslI     NarI     NruI     NspI
    PflMI     PfoI     PstI     SacI     SbfI     SfcI     SfoI     SmaI
     SphI     StuI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoRI     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     MfeI     MluI
     MmeI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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