Restriction map for pF1KB7706
 (Linear) (Six-base) MAPSORT of: KIBB7706.seq  Check: 4513  from: 1  to: 1395



KIBB7706.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AatII G_ACGT'C

Cuts at:      0       7    1395 
   Size:          7    1388
 
AccI GT'mk_AC

Cuts at:      0     824    1395 
   Size:        824     571
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1315    1395 
   Size:       1315      80
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     983    1015    1395 
   Size:        983      32     380

  Fragments arranged by size: 

                 983     380      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1130    1173    1338    1395 
   Size:       1130      43     165      57

  Fragments arranged by size: 

                1130     165      57      43
 
ApoI r'AATT_y

Cuts at:      0     331     436    1142    1395 
   Size:        331     105     706     253

  Fragments arranged by size: 

                 706     331     253     105
 
BanI G'GyrC_C

Cuts at:      0     139    1395 
   Size:        139    1256
 
BanII G_rGCy'C

Cuts at:      0    1217    1366    1395 
   Size:       1217     149      29

  Fragments arranged by size: 

                1217     149      29
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     717    1395 
   Size:        717     678
 
BclI T'GATC_A

Cuts at:      0     690    1395 
   Size:        690     705
 
BfrBI ATG'CAT

Cuts at:      0     865    1395 
   Size:        865     530
 
BglII A'GATC_T

Cuts at:      0     208    1395 
   Size:        208    1187
 
BlpI GC'TnA_GC

Cuts at:      0    1166    1395 
   Size:       1166     229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     752    1395 
   Size:        752     643
 
BmtI G_CTAG'C

Cuts at:      0     596    1395 
   Size:        596     799
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     395    1395 
   Size:        175     220    1000

  Fragments arranged by size: 

                1000     220     175
 
Bpu10I CC'TnA_GC

Cuts at:      0     478    1395 
   Size:        478     917
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186    1395 
   Size:        186    1209
 
BsaHI Gr'CG_yC

Cuts at:      0       4     222    1395 
   Size:          4     218    1173

  Fragments arranged by size: 

                1173     218       4
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     651     681    1395 
   Size:        651      30     714

  Fragments arranged by size: 

                 714     651      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91      94     656    1395 
   Size:         91       3     562     739

  Fragments arranged by size: 

                 739     562      91       3
 
BseYI C'CCAG_C

Cuts at:      0     615     651     713    1123    1395 
   Size:        615      36      62     410     272

  Fragments arranged by size: 

                 615     410     272      62      36
 
BsiHKAI G_wGCw'C

Cuts at:      0    1217    1395 
   Size:       1217     178
 
BsmI GAATG_Cn'

Cuts at:      0    1042    1395 
   Size:       1042     353
 
Bsp1286I G_dGCh'C

Cuts at:      0    1217    1366    1395 
   Size:       1217     149      29

  Fragments arranged by size: 

                1217     149      29
 
BspHI T'CATG_A

Cuts at:      0     303    1395 
   Size:        303    1092
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     219    1395 
   Size:        219    1176
 
BsrFI r'CCGG_y

Cuts at:      0    1274    1395 
   Size:       1274     121
 
BsrGI T'GTAC_A

Cuts at:      0    1062    1395 
   Size:       1062     333
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     106    1395 
   Size:        106    1289
 
BstYI r'GATC_y

Cuts at:      0     208     343     900    1395 
   Size:        208     135     557     495

  Fragments arranged by size: 

                 557     495     208     135
 
BtgI C'CryG_G

Cuts at:      0     143    1191    1395 
   Size:        143    1048     204

  Fragments arranged by size: 

                1048     204     143
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     635    1395 
   Size:        635     760
 
EaeI y'GGCC_r

Cuts at:      0      11     283    1395 
   Size:         11     272    1112

  Fragments arranged by size: 

                1112     272      11
 
EarI CTCTTCn'nnn_

Cuts at:      0     791    1334    1395 
   Size:        791     543      61

  Fragments arranged by size: 

                 791     543      61
 
EcoICRI GAG'CTC

Cuts at:      0    1215    1395 
   Size:       1215     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     395    1315    1395 
   Size:        175     220     920      80

  Fragments arranged by size: 

                 920     220     175      80
 
EcoRI G'AATT_C

Cuts at:      0     436    1395 
   Size:        436     959
 
HaeII r_GCGC'y

Cuts at:      0     282    1395 
   Size:        282    1113
 
HincII GTy'rAC

Cuts at:      0    1188    1395 
   Size:       1188     207
 
HindIII A'AGCT_T

Cuts at:      0     854    1395 
   Size:        854     541
 
MfeI C'AATT_G

Cuts at:      0     965    1395 
   Size:        965     430
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     828    1282    1395 
   Size:        299     529     454     113

  Fragments arranged by size: 

                 529     454     299     113
 
MscI TGG'CCA

Cuts at:      0      13    1395 
   Size:         13    1382
 
MslI CAynn'nnrTG

Cuts at:      0    1222    1237    1319    1395 
   Size:       1222      15      82      76

  Fragments arranged by size: 

                1222      82      76      15
 
MspA1I CmG'CkG

Cuts at:      0     655    1130    1395 
   Size:        655     475     265

  Fragments arranged by size: 

                 655     475     265
 
NcoI C'CATG_G

Cuts at:      0     143    1191    1395 
   Size:        143    1048     204

  Fragments arranged by size: 

                1048     204     143
 
NheI G'CTAG_C

Cuts at:      0     592    1395 
   Size:        592     803
 
NsiI A_TGCA'T

Cuts at:      0     867    1395 
   Size:        867     528
 
NspI r_CATG'y

Cuts at:      0     501    1395 
   Size:        501     894
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     720    1395 
   Size:        720     675
 
PfoI T'CCnGG_A

Cuts at:      0     174    1395 
   Size:        174    1221
 
PstI C_TGCA'G

Cuts at:      0     230    1135    1395 
   Size:        230     905     260

  Fragments arranged by size: 

                 905     260     230
 
PvuII CAG'CTG

Cuts at:      0     655    1130    1395 
   Size:        655     475     265

  Fragments arranged by size: 

                 655     475     265
 
SacI G_AGCT'C

Cuts at:      0    1217    1395 
   Size:       1217     178
 
SapI GCTCTTCn'nnn_

Cuts at:      0     791    1395 
   Size:        791     604
 
SbfI CC_TGCA'GG

Cuts at:      0     230    1395 
   Size:        230    1165
 
SfcI C'TryA_G

Cuts at:      0     226    1131    1395 
   Size:        226     905     264

  Fragments arranged by size: 

                 905     264     226
 
SmlI C'TyrA_G

Cuts at:      0     165    1395 
   Size:        165    1230
 
SpeI A'CTAG_T

Cuts at:      0      31     874    1395 
   Size:         31     843     521

  Fragments arranged by size: 

                 843     521      31
 
StyI C'CwwG_G

Cuts at:      0     143    1191    1395 
   Size:        143    1048     204

  Fragments arranged by size: 

                1048     204     143
 
TatI w'GTAC_w

Cuts at:      0    1007    1062    1395 
   Size:       1007      55     333

  Fragments arranged by size: 

                1007     333      55
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      54      86     537     569    1395 
   Size:         54      32     451      32     826

  Fragments arranged by size: 

                 826     451      54      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     150    1395 
   Size:        150    1245
 
XmnI GAAnn'nnTTC

Cuts at:      0     857    1395 
   Size:        857     538
 
ZraI GAC'GTC

Cuts at:      0       5    1395 
   Size:          5    1390
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AloI    AlwNI     ApoI     BanI    BanII
    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI    BsrGI   BstAPI    BstYI     BtgI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoRI    HaeII   HincII
  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NheI
     NsiI     NspI    PflMI     PfoI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     SmlI     SpeI     StyI     TatI     TstI     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI     BglI
 Bme1580I    BmgBI     BplI     BsaI    BsaAI    BsaBI    BsaWI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EciI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I     HpaI     KasI     KpnI
     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI
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