Restriction map for pF1KE0346
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BamHI       BclI        BfrI        BglII       BlnI        
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SmlI        
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         565  CGCG                 CG^CG
AcsI          271  GAATTT               [GA]^AATT[CT]
AflIII        282  ACGTGT               A^C[GA][CT]GT
AhdI         1156  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1078  GTGCAC               G^TGCAC
AlwI         1623  GATCC                GGATC(4/5)
AocI         1383  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1078  GTGCAC               G^TGCAC
ApoI          271  GAATTT               [GA]^AATT[CT]
AspEI        1156  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          388  GGCGCC               GGCGC^C
BbvCI        1460  CCTCAGC              CCTCAGC(-5/-2)
BciVI         322  GTATCC               GTATCC(6/5)
BfaI           26  CTAG                 C^TAG
BsmBI          88  GAGACG               CGTCTC(1/5)
Bsp1407I     1341  TGTACA               T^GTACA
BspMI         480  GCAGGT               ACCTGC(4/8)
BsrFI         715  GCCGGC               [GA]^CCGG[CT]
BsrGI        1341  TGTACA               T^GTACA
BstUI         565  CGCG                 CG^CG
BstXI         188  CCAAGGAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1622  AGATCC               [GA]^GATC[CT]
Bsu36I       1383  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        715  GCCGGC               [GA]^CCGG[CT]
DpnII        1623  GATC                 ^GATC
DraIII       1810  CACTACGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1156  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1383  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1357  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI          707  GCGTC                GACGC(5/10)
KasI          388  GGCGCC               G^GCGCC
MaeI           26  CTAG                 C^TAG
MboI         1623  GATC                 ^GATC
MflI         1622  AGATCC               [GA]^GATC[CT]
MvnI          565  CGCG                 CG^CG
NaeI          715  GCCGGC               GCC^GGC
NarI          388  GGCGCC               GG^CGCC
NdeI         1897  CATATG               CA^TATG
NdeII        1623  GATC                 ^GATC
NgoMIV        715  GCCGGC               G^CCGGC
NheI           25  GCTAGC               G^CTAGC
PpuMI        1886  AGGACCC              [GA]G^G[AT]CC[CT]
SacI         1305  GAGCTC               GAGCT^C
SapI         1053  GCTCTTC              GCTCTTC(1/4)
Sau3AI       1623  GATC                 ^GATC
SfiI          231  GGCCGCTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          388  GGCGCC               GGC^GCC
SmaI            5  CCCGGG               CCC^GGG
SnaBI         319  TACGTA               TAC^GTA
SspBI        1341  TGTACA               T^GTACA
TfiI         1641  GATTC                G^A[AT]TC
Tsp509I       272  AATT                 ^AATT
XhoII        1622  AGATCC               [GA]^GATC[CT]
XmaCI           5  CCCGGG               C^CCGGG
XmaI            5  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AatII          90  GACGTC               GACGT^C
              754  GACGTC              
AfaI         1342  GTAC                 GT^AC
             1413  GTAC                
AlwNI          46  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1174  CAGTTCCTG           
Aor51HI       667  AGCGCT               AGC^GCT
              909  AGCGCT              
AvaI            5  CCCGGG               C^[CT]CG[GA]G
              156  CTCGGG              
BbrPI         281  CACGTG               CAC^GTG
              466  CACGTG              
BbsI         1609  GAAGAC               GAAGAC(2/6)
             1248  GTCTTC              
BcgI          753  CGACGTCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1596  GCAACAGTATCG        
BlpI          999  GCTCAGC              GC^T[ACGT]AGC
             1566  GCTCAGC             
Bpu1102I      999  GCTCAGC              GC^T[ACGT]AGC
             1566  GCTCAGC             
BpuAI        1609  GAAGAC               GAAGAC(2/6)
             1248  GTCTTC              
BsaI          460  GAGACC               GGTCTC(1/5)
              160  GGTCTC              
BsgI         1507  CTGCAC               GTGCAG(16/14)
             1849  GTGCAG              
BsoBI           5  CCCGGG               C^[CT]CG[GA]G
              156  CTCGGG              
BstAPI        835  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1851  GCAGGCTATGC         
CelII         999  GCTCAGC              GC^T[ACGT]AGC
             1566  GCTCAGC             
DraII        1387  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1886  AGGACCC             
Eco47III      667  AGCGCT               AGC^GCT
              909  AGCGCT              
EcoO109I     1387  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1886  AGGACCC             
MslI           35  CACGCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1206  CACATTCGTG          
PmaCI         281  CACGTG               CAC^GTG
              466  CACGTG              
PmlI          281  CACGTG               CAC^GTG
              466  CACGTG              
RsaI         1342  GTAC                 GT^AC
             1413  GTAC                
SfcI          939  CTACAG               C^T[GA][CT]AG
             1324  CTACAG              
Tsp45I         93  GTCAC                ^GT[GC]AC
             1432  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcyI        AluI        AspHI       AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaAI       BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DsaI        EaeI        EarI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboII       MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NlaIII      NlaIV       
PleI        PvuII       Sau96I      ScrFI       SfaNI       
StyI        TaqI        TseI        TspRI       TthHB8I     
XcmI        
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