Restriction map for pF1KE0346
 (Circular) (Six-base) MAPSORT of: pF1KE0346.seq  Check: 8745  from: 1  to: 5018



pF1KE0346  5018 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    579     579 
   Size:       5018
 
AatII G_ACGT'C

Cuts at:    202     866     202 
   Size:        664    4354
 
AccI GT'mk_AC

Cuts at:   2056    2056 
   Size:       5018
 
Acc65I G'GTAC_C

Cuts at:   2034    2034 
   Size:       5018
 
AclI AA'CG_TT

Cuts at:   4858    4858 
   Size:       5018
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    190    1501    2843    3275    4022    4290     190 
   Size:       1311    1342     432     747     268     918

  Fragments arranged by size: 

                1342    1311     918     747     432     268
 
AfeI AGC'GCT

Cuts at:    777    1019     777 
   Size:        242    4776
 
AflIII A'CryG_T

Cuts at:    390    2370    3474    4451     390 
   Size:       1980    1104     977     957

  Fragments arranged by size: 

                1980    1104     977     957
 
AgeI A'CCGG_T

Cuts at:   3406    3406 
   Size:       5018
 
AhdI GACnn_n'nnGTC

Cuts at:   1269    1269 
   Size:       5018
 
AleI CACnn'nnGTG

Cuts at:   1318    1318 
   Size:       5018
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    165     384     165 
   Size:        219    4799
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    523     555     523 
   Size:         32    4986
 
AlwNI CAG_nnn'CTG

Cuts at:    159    1287    2323    3890     159 
   Size:       1128    1036    1567    1287

  Fragments arranged by size: 

                1567    1287    1128    1036
 
ApaLI G'TGCA_C

Cuts at:   1186    3788    1186 
   Size:       2602    2416
 
ApoI r'AATT_y

Cuts at:    379    2022    4371    4627     379 
   Size:       1643    2349     256     770

  Fragments arranged by size: 

                2349    1643     770     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5018
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5018
 
AvaI C'yCGr_G

Cuts at:    113     264    2038     113 
   Size:        151    1774    3093

  Fragments arranged by size: 

                3093    1774     151
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    413     446     413 
   Size:         33    4985
 
BamHI G'GATC_C

Cuts at:   2043    2043 
   Size:       5018
 
BanI G'GyrC_C

Cuts at:    286     496     591     949     987    1203    1216    1983    2034 
   Size:        210      95     358      38     216      13     767      51

Cuts at:   2034    2448    2696    2731     286 
   Size:        414     248      35    2573

  Fragments arranged by size: 

                2573     767     414     358     248     216     210      95
                  51      38      35      13
 
BanII G_rGCy'C

Cuts at:    265     409     672    1163    1235    1417    1698    2032    3062 
   Size:        144     263     491      72     182     281     334    1030

Cuts at:   3062     265 
   Size:       2221

  Fragments arranged by size: 

                2221    1030     491     334     281     263     182     144
                  72
 
BbeI G_GCGC'C

Cuts at:    500    2452    2700     500 
   Size:       1952     248    2818

  Fragments arranged by size: 

                2818    1952     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1349    1724    1349 
   Size:        375    4643
 
BbvCI CC'TCA_GC

Cuts at:   1569    1569 
   Size:       5018
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    850     884    1693    1727     850 
   Size:         34     809      34    4141

  Fragments arranged by size: 

                4141     809      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    441    2916    3677    5018     441 
   Size:       2475     761    1341     441

  Fragments arranged by size: 

                2475    1341     761     441
 
BclI T'GATC_A

Cuts at:   2538    2538 
   Size:       5018
 
BglI GCCn_nnn'nGGC

Cuts at:    346     505    1249     346 
   Size:        159     744    4115

  Fragments arranged by size: 

                4115     744     159
 
BglII A'GATC_T

Cuts at:   2533    4570    2533 
   Size:       2037    2981
 
BlpI GC'TnA_GC

Cuts at:   1108    1675    2130    1108 
   Size:        567     455    3996

  Fragments arranged by size: 

                3996     567     455
 
Bme1580I G_kGCm'C

Cuts at:    390     596     992    1190    1219    1447    2643    2736    3792 
   Size:        206     396     198      29     228    1196      93    1056

Cuts at:   3792     390 
   Size:       1616

  Fragments arranged by size: 

                1616    1196    1056     396     228     206     198      93
                  29
 
BmrI ACTGGGnnnn_n'

Cuts at:   1638    2230    2311    2646    4790    1638 
   Size:        592      81     335    2144    1866

  Fragments arranged by size: 

                2144    1866     592     335      81
 
BmtI G_CTAG'C

Cuts at:    137     137 
   Size:       5018
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3385    3385 
   Size:       5018
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     537     792     861    1288    1550    1966     199 
   Size:        338     255      69     427     262     416    3251

  Fragments arranged by size: 

                3251     427     416     338     262     255      69
 
Bpu10I CC'TnA_GC

Cuts at:   1466    1569    1759    1466 
   Size:        103     190    4725

  Fragments arranged by size: 

                4725     190     103
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2194    3073    3565    3863    2194 
   Size:        879     492     298    3349

  Fragments arranged by size: 

                3349     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    274     562    4722     274 
   Size:        288    4160     570

  Fragments arranged by size: 

                4160     570     288
 
BsaAI yAC'GTr

Cuts at:    276     391     429     576    1923    2371     276 
   Size:        115      38     147    1347     448    2923

  Fragments arranged by size: 

                2923    1347     448     147     115      38
 
BsaBI GATnn'nnATC

Cuts at:      6    2532    4569       6 
   Size:       2526    2037     455

  Fragments arranged by size: 

                2526    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    199     497     815     863    2449    2697    3399    4895     199 
   Size:        298     318      48    1586     248     702    1496     322

  Fragments arranged by size: 

                1586    1496     702     322     318     298     248      48
            
 
BsaWI w'CCGG_w

Cuts at:   2207    2728    3406    3680    3827    4238    2207 
   Size:        521     678     274     147     411    2987

  Fragments arranged by size: 

                2987     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    659     689    1523    1553     659 
   Size:         30     834      30    4124

  Fragments arranged by size: 

                4124     834      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    466     898    1105    1225    1963     466 
   Size:        432     207     120     738    3521

  Fragments arranged by size: 

                3521     738     432     207     120
 
BseYI C'CCAG_C

Cuts at:    758    1366    1380    1975    2443    3201    3778    4274     758 
   Size:        608      14     595     468     758     577     496    1502

  Fragments arranged by size: 

                1502     758     608     595     577     496     468      14
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1600    1978    1600 
   Size:        378    4640
 
BsiEI CG_ry'CG

Cuts at:    104    2560    2606    3814     104 
   Size:       2456      46    1208    1308

  Fragments arranged by size: 

                2456    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    281     323    1190    1417    2032    2810    3000    3792     281 
   Size:         42     867     227     615     778     190     792    1507

  Fragments arranged by size: 

                1507     867     792     778     615     227     190      42
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    190     190 
   Size:       5018
 
Bsp1286I G_dGCh'C

Cuts at:    265     281     323     390     409     596     672     992    1163 
   Size:         16      42      67      19     187      76     320     171

Cuts at:   1163    1190    1219    1235    1417    1447    1698    2032    2643 
   Size:         27      29      16     182      30     251     334     611

Cuts at:   2643    2736    2810    3000    3062    3792     265 
   Size:         93      74     190      62     730    1491

  Fragments arranged by size: 

                1491     730     611     334     320     251     190     187
                 182     171      93      76      74      67      62      42
                  30      29      27      19      16      16
 
BspEI T'CCGG_A

Cuts at:   4238    4238 
   Size:       5018
 
BspHI T'CATG_A

Cuts at:   4194    4194 
   Size:       5018
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    579    2068    2282    2584    2965     579 
   Size:       1489     214     302     381    2632

  Fragments arranged by size: 

                2632    1489     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3310    3364    4848    5013      50 
   Size:       3260      54    1484     165      55

  Fragments arranged by size: 

                3260    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    128     866     938    2930     128 
   Size:        738      72    1992    2216

  Fragments arranged by size: 

                2216    1992     738      72
 
BsrFI r'CCGG_y

Cuts at:    823    3016    3197    3406     823 
   Size:       2193     181     209    2435

  Fragments arranged by size: 

                2435    2193     209     181
 
BsrGI T'GTAC_A

Cuts at:   1449    1449 
   Size:       5018
 
BssSI C'ACGA_G

Cuts at:   3289    3647    3289 
   Size:        358    4660
 
BstAPI GCAn_nnn'nTGC

Cuts at:    949    1965    2395     949 
   Size:       1016     430    3572

  Fragments arranged by size: 

                3572    1016     430
 
BstBI TT'CG_AA

Cuts at:   3379    3379 
   Size:       5018
 
BstXI CCAn_nnnn'nTGG

Cuts at:    303     303 
   Size:       5018
 
BstYI r'GATC_y

Cuts at:      1    1730    2043    2510    2533    2868    3114    4126    4212 
   Size:       1729     313     467      23     335     246    1012      86

Cuts at:   4212    4224    4570       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1729    1012     467     449     346     335     313     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1492    1492 
   Size:       5018
 
BtgI C'CryG_G

Cuts at:    107     290     434     503     898    1145    1736     107 
   Size:        183     144      69     395     247     591    3389

  Fragments arranged by size: 

                3389     591     395     247     183     144      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    906    2968    3149    3312     906 
   Size:       2062     181     163    2612

  Fragments arranged by size: 

                2612    2062     181     163
 
BtsI GCAGTG_nn'

Cuts at:    202     827    1252    1541    1853    2261     202 
   Size:        625     425     289     312     408    2959

  Fragments arranged by size: 

                2959     625     425     408     312     289
 
DraI TTT'AAA

Cuts at:   2017    2017 
   Size:       5018
 
DraIII CAC_nnn'GTG

Cuts at:   1923    1923 
   Size:       5018
 
DrdI GACnn_nn'nnGTC

Cuts at:   2724    3582    2724 
   Size:        858    4160
 
EaeI y'GGCC_r

Cuts at:    293     338     704     821     917    1148    1322    2001    2603 
   Size:         45     366     117      96     231     174     679     602

Cuts at:   2603    2777    3168    3195    4540     293 
   Size:        174     391      27    1345     771

  Fragments arranged by size: 

                1345     771     679     602     391     366     231     174
                 174     117      96      45      27
 
EagI C'GGCC_G

Cuts at:   2603    2603 
   Size:       5018
 
EarI CTCTTCn'nnn_

Cuts at:    242     733    1123    1168    3041    3251     242 
   Size:        491     390      45    1873     210    2009

  Fragments arranged by size: 

                2009    1873     491     390     210      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3226    3536    3682    4828    3226 
   Size:        310     146    1146    3416

  Fragments arranged by size: 

                3416    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1415    2030    1415 
   Size:        615    4403
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    190     199     537     792     861    1288    1501    1550    1966 
   Size:          9     338     255      69     427     213      49     416

Cuts at:   1966    2843    3275    4022    4290     190 
   Size:        877     432     747     268     918

  Fragments arranged by size: 

                 918     877     747     432     427     416     338     268
                 255     213      69      49       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1469    1469 
   Size:       5018
 
EcoO109I rG'GnC_Cy

Cuts at:   1496    1995    2157    1496 
   Size:        499     162    4357

  Fragments arranged by size: 

                4357     499     162
 
EcoRI G'AATT_C

Cuts at:   2022    2022 
   Size:       5018
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3037    3037 
   Size:       5018
 
FspI TGC'GCA

Cuts at:   2799    2799 
   Size:       5018
 
HaeII r_GCGC'y

Cuts at:    213     500     779    1021    1552    2452    2700    3722    4701 
   Size:        287     279     242     531     900     248    1022     979

Cuts at:   4701     213 
   Size:        530

  Fragments arranged by size: 

                1022     979     900     531     530     287     279     248
                 242
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    997    1029    1255    1287    2860    2890    2892    2922     997 
   Size:         32     226      32    1573      30       2      30    3093

  Fragments arranged by size: 

                3093    1573     226      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2057    2057 
   Size:       5018
 
KasI G'GCGC_C

Cuts at:    496    2448    2696     496 
   Size:       1952     248    2818

  Fragments arranged by size: 

                2818    1952     248
 
KpnI G_GTAC'C

Cuts at:   2038    2038 
   Size:       5018
 
MluI A'CGCG_T

Cuts at:   4451    4451 
   Size:       5018
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3689    3873    3689 
   Size:        184    4834
 
MscI TGG'CCA

Cuts at:    295     919    1150    1324    2003    2779     295 
   Size:        624     231     174     679     776    2534

  Fragments arranged by size: 

                2534     776     679     624     231     174
 
MslI CAynn'nnrTG

Cuts at:    147    1318    3007    3134     147 
   Size:       1171    1689     127    2031

  Fragments arranged by size: 

                2031    1689    1171     127
 
MspA1I CmG'CkG

Cuts at:    177     343     946    1041    1075    1437    1890    2129    2443 
   Size:        166     603      95      34     362     453     239     314

Cuts at:   2443    2803    3816    4061     177 
   Size:        360    1013     245    1134

  Fragments arranged by size: 

                1134    1013     603     453     362     360     314     245
                 239     166      95      34
 
NaeI GCC'GGC

Cuts at:    825    3199     825 
   Size:       2374    2644
 
NarI GG'CG_CC

Cuts at:    497    2449    2697     497 
   Size:       1952     248    2818

  Fragments arranged by size: 

                2818    1952     248
 
NcoI C'CATG_G

Cuts at:    107     434    1145    1736     107 
   Size:        327     711     591    3389

  Fragments arranged by size: 

                3389     711     591     327
 
NdeI CA'TA_TG

Cuts at:   2006    2006 
   Size:       5018
 
NgoMIV G'CCGG_C

Cuts at:    823    3197     823 
   Size:       2374    2644
 
NheI G'CTAG_C

Cuts at:    133     133 
   Size:       5018
 
NspI r_CATG'y

Cuts at:   2071    2571    3478    2071 
   Size:        500     907    3611

  Fragments arranged by size: 

                3611     907     500
 
PasI CC'CwG_GG

Cuts at:   1934    1934 
   Size:       5018
 
PciI A'CATG_T

Cuts at:   3474    3474 
   Size:       5018
 
PmeI GTTT'AAAC

Cuts at:   2017    2017 
   Size:       5018
 
PmlI CAC'GTG

Cuts at:    391     576     391 
   Size:        185    4833
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4157    4189    4157 
   Size:         32    4986
 
PpuMI rG'GwC_Cy

Cuts at:   1995    1995 
   Size:       5018
 
PstI C_TGCA'G

Cuts at:   2065    2750    2065 
   Size:        685    4333
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5018
 
PvuII CAG'CTG

Cuts at:    946    1075    1437    2443    2803     946 
   Size:        129     362    1006     360    3161

  Fragments arranged by size: 

                3161    1006     362     360     129
 
RsrII CG'GwC_CG

Cuts at:   3213    3213 
   Size:       5018
 
SacI G_AGCT'C

Cuts at:   1417    2032    1417 
   Size:        615    4403
 
SalI G'TCGA_C

Cuts at:   2055    2055 
   Size:       5018
 
SapI GCTCTTCn'nnn_

Cuts at:   1168    3041    3251    1168 
   Size:       1873     210    2935

  Fragments arranged by size: 

                2935    1873     210
 
SbfI CC_TGCA'GG

Cuts at:   2065    2065 
   Size:       5018
 
ScaI AGT'ACT

Cuts at:   4535    4535 
   Size:       5018
 
SfcI C'TryA_G

Cuts at:     33    1047    1432    2061    2746    3739    3930      33 
   Size:       1014     385     629     685     993     191    1121

  Fragments arranged by size: 

                1121    1014     993     685     629     385     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    346     346 
   Size:       5018
 
SfoI GGC'GCC

Cuts at:    498    2450    2698     498 
   Size:       1952     248    2818

  Fragments arranged by size: 

                2818    1952     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5018
 
SmaI CCC'GGG

Cuts at:    115    2040     115 
   Size:       1925    3093
 
SmlI C'TyrA_G

Cuts at:   2173    3088    3580    3842    2173 
   Size:        915     492     262    3349

  Fragments arranged by size: 

                3349     915     492     262
 
SnaBI TAC'GTA

Cuts at:    429     429 
   Size:       5018
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5018
 
SphI G_CATG'C

Cuts at:   2071    2571    2071 
   Size:        500    4518
 
StyI C'CwwG_G

Cuts at:    107     296     434    1145    1293    1736    2152    2506     107 
   Size:        189     138     711     148     443     416     354    2619

  Fragments arranged by size: 

                2619     711     443     416     354     189     148     138
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    238    1800    2601    2734    3193    3402     238 
   Size:       1562     801     133     459     209    1854

  Fragments arranged by size: 

                1854    1562     801     459     209     133
 
TatI w'GTAC_w

Cuts at:   1449    4533    1449 
   Size:       3084    1934
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    659     691    1523    1555    1832    1864    4535    4567     659 
   Size:         32     832      32     277      32    2671      32    1110

  Fragments arranged by size: 

                2671    1110     832     277      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   2815    2815 
   Size:       5018
 
XbaI T'CTAG_A

Cuts at:   2049    2049 
   Size:       5018
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    135    1920    1941    2513     135 
   Size:       1785      21     572    2640

  Fragments arranged by size: 

                2640    1785     572      21
 
XmaI C'CCGG_G

Cuts at:    113    2038     113 
   Size:       1925    3093
 
ZraI GAC'GTC

Cuts at:    200     864     200 
   Size:        664    4354
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NspI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

    AflII     ApaI     AscI    AvrII    BfrBI    BmgBI    BsiWI     BsmI
   BssHII   BstEII  BstZ17I     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NotI     NruI     NsiI     PacI    PflMI
     PfoI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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