Restriction map for pF1KE0346
 (Linear) (Six-base) MAPSORT of: KIEE0346.seq  Check: 7980  from: 1  to: 1905



KIEE0346.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     471    1905 
   Size:        471    1434
 
AatII G_ACGT'C

Cuts at:      0      94     758    1905 
   Size:         94     664    1147

  Fragments arranged by size: 

                1147     664      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1393    1905 
   Size:         82    1311     512

  Fragments arranged by size: 

                1311     512      82
 
AfeI AGC'GCT

Cuts at:      0     669     911    1905 
   Size:        669     242     994

  Fragments arranged by size: 

                 994     669     242
 
AflIII A'CryG_T

Cuts at:      0     282    1905 
   Size:        282    1623
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1161    1905 
   Size:       1161     744
 
AleI CACnn'nnGTG

Cuts at:      0    1210    1905 
   Size:       1210     695
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      57     276    1905 
   Size:         57     219    1629

  Fragments arranged by size: 

                1629     219      57
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     415     447    1905 
   Size:        415      32    1458

  Fragments arranged by size: 

                1458     415      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51    1179    1905 
   Size:         51    1128     726

  Fragments arranged by size: 

                1128     726      51
 
ApaLI G'TGCA_C

Cuts at:      0    1078    1905 
   Size:       1078     827
 
ApoI r'AATT_y

Cuts at:      0     271    1905 
   Size:        271    1634
 
AvaI C'yCGr_G

Cuts at:      0       5     156    1905 
   Size:          5     151    1749

  Fragments arranged by size: 

                1749     151       5
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     305     338    1905 
   Size:        305      33    1567

  Fragments arranged by size: 

                1567     305      33
 
BanI G'GyrC_C

Cuts at:      0     178     388     483     841     879    1095    1108    1875 
   Size:        178     210      95     358      38     216      13     767

Cuts at:   1875    1905 
   Size:         30

  Fragments arranged by size: 

                 767     358     216     210     178      95      38      30
                  13
 
BanII G_rGCy'C

Cuts at:      0     157     301     564    1055    1127    1309    1590    1905 
   Size:        157     144     263     491      72     182     281     315

  Fragments arranged by size: 

                 491     315     281     263     182     157     144      72
            
 
BbeI G_GCGC'C

Cuts at:      0     392    1905 
   Size:        392    1513
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1241    1616    1905 
   Size:       1241     375     289

  Fragments arranged by size: 

                1241     375     289
 
BbvCI CC'TCA_GC

Cuts at:      0    1461    1905 
   Size:       1461     444
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     742     776    1585    1619    1905 
   Size:        742      34     809      34     286

  Fragments arranged by size: 

                 809     742     286      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     333    1905 
   Size:        333    1572
 
BglI GCCn_nnn'nGGC

Cuts at:      0     238     397    1141    1905 
   Size:        238     159     744     764

  Fragments arranged by size: 

                 764     744     238     159
 
BlpI GC'TnA_GC

Cuts at:      0    1000    1567    1905 
   Size:       1000     567     338

  Fragments arranged by size: 

                1000     567     338
 
Bme1580I G_kGCm'C

Cuts at:      0     282     488     884    1082    1111    1339    1905 
   Size:        282     206     396     198      29     228     566

  Fragments arranged by size: 

                 566     396     282     228     206     198      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1530    1905 
   Size:       1530     375
 
BmtI G_CTAG'C

Cuts at:      0      29    1905 
   Size:         29    1876
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     429     684     753    1180    1442    1858    1905 
   Size:         91     338     255      69     427     262     416      47

  Fragments arranged by size: 

                 427     416     338     262     255      91      69      47
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1358    1461    1651    1905 
   Size:       1358     103     190     254

  Fragments arranged by size: 

                1358     254     190     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     166     454    1905 
   Size:        166     288    1451

  Fragments arranged by size: 

                1451     288     166
 
BsaAI yAC'GTr

Cuts at:      0     168     283     321     468    1815    1905 
   Size:        168     115      38     147    1347      90

  Fragments arranged by size: 

                1347     168     147     115      90      38
 
BsaHI Gr'CG_yC

Cuts at:      0      91     389     707     755    1905 
   Size:         91     298     318      48    1150

  Fragments arranged by size: 

                1150     318     298      91      48
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     551     581    1415    1445    1905 
   Size:        551      30     834      30     460

  Fragments arranged by size: 

                 834     551     460      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     790     997    1117    1855    1905 
   Size:        358     432     207     120     738      50

  Fragments arranged by size: 

                 738     432     358     207     120      50
 
BseYI C'CCAG_C

Cuts at:      0     650    1258    1272    1867    1905 
   Size:        650     608      14     595      38

  Fragments arranged by size: 

                 650     608     595      38      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1492    1870    1905 
   Size:       1492     378      35

  Fragments arranged by size: 

                1492     378      35
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     215    1082    1309    1905 
   Size:        173      42     867     227     596

  Fragments arranged by size: 

                 867     596     227     173      42
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      82    1905 
   Size:         82    1823
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     173     215     282     301     488     564     884 
   Size:        157      16      42      67      19     187      76     320

Cuts at:    884    1055    1082    1111    1127    1309    1339    1590    1905 
   Size:        171      27      29      16     182      30     251     315

  Fragments arranged by size: 

                 320     315     251     187     182     171     157      76
                  67      42      30      29      27      19      16      16
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     471    1905 
   Size:        471    1434
 
BsrDI GCAATG_nn'

Cuts at:      0      20     758     830    1905 
   Size:         20     738      72    1075

  Fragments arranged by size: 

                1075     738      72      20
 
BsrFI r'CCGG_y

Cuts at:      0     715    1905 
   Size:        715    1190
 
BsrGI T'GTAC_A

Cuts at:      0    1341    1905 
   Size:       1341     564
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     841    1857    1905 
   Size:        841    1016      48

  Fragments arranged by size: 

                1016     841      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195    1905 
   Size:        195    1710
 
BstYI r'GATC_y

Cuts at:      0    1622    1905 
   Size:       1622     283
 
Bsu36I CC'TnA_GG

Cuts at:      0    1384    1905 
   Size:       1384     521
 
BtgI C'CryG_G

Cuts at:      0     182     326     395     790    1037    1628    1905 
   Size:        182     144      69     395     247     591     277

  Fragments arranged by size: 

                 591     395     277     247     182     144      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     798    1905 
   Size:        798    1107
 
BtsI GCAGTG_nn'

Cuts at:      0      94     719    1144    1433    1745    1905 
   Size:         94     625     425     289     312     160

  Fragments arranged by size: 

                 625     425     312     289     160      94
 
DraIII CAC_nnn'GTG

Cuts at:      0    1815    1905 
   Size:       1815      90
 
EaeI y'GGCC_r

Cuts at:      0     185     230     596     713     809    1040    1214    1893 
   Size:        185      45     366     117      96     231     174     679

Cuts at:   1893    1905 
   Size:         12

  Fragments arranged by size: 

                 679     366     231     185     174     117      96      45
                  12
 
EarI CTCTTCn'nnn_

Cuts at:      0     134     625    1015    1060    1905 
   Size:        134     491     390      45     845

  Fragments arranged by size: 

                 845     491     390     134      45
 
EcoICRI GAG'CTC

Cuts at:      0    1307    1905 
   Size:       1307     598
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82      91     429     684     753    1180    1393    1442 
   Size:         82       9     338     255      69     427     213      49

Cuts at:   1442    1858    1905 
   Size:        416      47

  Fragments arranged by size: 

                 427     416     338     255     213      82      69      49
                  47       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1361    1905 
   Size:       1361     544
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1388    1887    1905 
   Size:       1388     499      18

  Fragments arranged by size: 

                1388     499      18
 
HaeII r_GCGC'y

Cuts at:      0     105     392     671     913    1444    1905 
   Size:        105     287     279     242     531     461

  Fragments arranged by size: 

                 531     461     287     279     242     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     889     921    1147    1179    1905 
   Size:        889      32     226      32     726

  Fragments arranged by size: 

                 889     726     226      32      32
 
KasI G'GCGC_C

Cuts at:      0     388    1905 
   Size:        388    1517
 
MscI TGG'CCA

Cuts at:      0     187     811    1042    1216    1895    1905 
   Size:        187     624     231     174     679      10

  Fragments arranged by size: 

                 679     624     231     187     174      10
 
MslI CAynn'nnrTG

Cuts at:      0      39    1210    1905 
   Size:         39    1171     695

  Fragments arranged by size: 

                1171     695      39
 
MspA1I CmG'CkG

Cuts at:      0      69     235     838     933     967    1329    1782    1905 
   Size:         69     166     603      95      34     362     453     123

  Fragments arranged by size: 

                 603     453     362     166     123      95      69      34
            
 
NaeI GCC'GGC

Cuts at:      0     717    1905 
   Size:        717    1188
 
NarI GG'CG_CC

Cuts at:      0     389    1905 
   Size:        389    1516
 
NcoI C'CATG_G

Cuts at:      0     326    1037    1628    1905 
   Size:        326     711     591     277

  Fragments arranged by size: 

                 711     591     326     277
 
NdeI CA'TA_TG

Cuts at:      0    1898    1905 
   Size:       1898       7
 
NgoMIV G'CCGG_C

Cuts at:      0     715    1905 
   Size:        715    1190
 
NheI G'CTAG_C

Cuts at:      0      25    1905 
   Size:         25    1880
 
PasI CC'CwG_GG

Cuts at:      0    1826    1905 
   Size:       1826      79
 
PmlI CAC'GTG

Cuts at:      0     283     468    1905 
   Size:        283     185    1437

  Fragments arranged by size: 

                1437     283     185
 
PpuMI rG'GwC_Cy

Cuts at:      0    1887    1905 
   Size:       1887      18
 
PvuII CAG'CTG

Cuts at:      0     838     967    1329    1905 
   Size:        838     129     362     576

  Fragments arranged by size: 

                 838     576     362     129
 
SacI G_AGCT'C

Cuts at:      0    1309    1905 
   Size:       1309     596
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1060    1905 
   Size:       1060     845
 
SfcI C'TryA_G

Cuts at:      0     939    1324    1905 
   Size:        939     385     581

  Fragments arranged by size: 

                 939     581     385
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     238    1905 
   Size:        238    1667
 
SfoI GGC'GCC

Cuts at:      0     390    1905 
   Size:        390    1515
 
SmaI CCC'GGG

Cuts at:      0       7    1905 
   Size:          7    1898
 
SnaBI TAC'GTA

Cuts at:      0     321    1905 
   Size:        321    1584
 
StyI C'CwwG_G

Cuts at:      0     188     326    1037    1185    1628    1905 
   Size:        188     138     711     148     443     277

  Fragments arranged by size: 

                 711     443     277     188     148     138
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     130    1692    1905 
   Size:        130    1562     213

  Fragments arranged by size: 

                1562     213     130
 
TatI w'GTAC_w

Cuts at:      0    1341    1905 
   Size:       1341     564
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     551     583    1415    1447    1724    1756    1905 
   Size:        551      32     832      32     277      32     149

  Fragments arranged by size: 

                 832     551     277     149      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      27    1812    1833    1905 
   Size:         27    1785      21      72

  Fragments arranged by size: 

                1785      72      27      21
 
XmaI C'CCGG_G

Cuts at:      0       5    1905 
   Size:          5    1900
 
ZraI GAC'GTC

Cuts at:      0      92     756    1905 
   Size:         92     664    1149

  Fragments arranged by size: 

                1149     664      92
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AvaI     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I
     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     KasI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     PasI     PmlI    PpuMI    PvuII
     SacI     SapI     SfcI     SfiI     SfoI     SmaI    SnaBI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     ApaI     AscI     AseI
    AsiSI    AvrII    BamHI     BclI    BfrBI    BglII    BmgBI     BplI
    BpuEI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrBI   BssHII    BssSI    BstBI   BstEII  BstZ17I     ClaI    CspCI
     DraI     DrdI     EagI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SgfI    SgrAI     SmlI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmnI
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