Restriction map for pF1KB6424
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT14I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NarI        NciI        NcoI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          653  GTAGAC               GT^[AC][GT]AC
AciI           42  GCGG                 CCGC(-3/-1)
AcyI          845  GGCGTC               G[GA]^CG[CT]C
AflIII        672  ACATGT               A^C[GA][CT]GT
AseI          234  ATTAAT               AT^TAAT
AsnI          234  ATTAAT               AT^TAAT
Asp700I       815  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         287  GGTCC                G^G[AT]CC
BciVI         190  GGATAC               GTATCC(6/5)
BpmI          551  CTGGAG               CTGGAG(16/14)
BsaHI         845  GGCGTC               G[GA]^CG[CT]C
BsaJI         293  CCTGGG               C^C[ACGT][ACGT]GG
BsmBI         847  CGTCTC               CGTCTC(1/5)
BsmI          546  GCATTC               GAATGC(1/-1)
BspLU11I      672  ACATGT               A^CATGT
BspMI         913  GCAGGT               ACCTGC(4/8)
BsrFI         749  GCCGGC               [GA]^CCGG[CT]
BstF5I         57  GGATG                GGATG(2/0)
Cfr10I        749  GCCGGC               [GA]^CCGG[CT]
DraII         286  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          618  CTCTTC               CTCTTC(1/4)
EcoO109I      286  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         682  GATATC               GAT^ATC
FokI           57  GGATG                GGATG(9/13)
HaeIII        157  GGCC                 GG^CC
HapII         750  CCGG                 C^CGG
HgaI          846  GCGTC                GACGC(5/10)
Hin1I         845  GGCGTC               G[GA]^CG[CT]C
HpaII         750  CCGG                 C^CGG
HphI          297  GGTGA                GGTGA(8/7)
Ksp632I       618  CTCTTC               CTCTTC(1/4)
MaeII         144  ACGT                 A^CGT
MboII         619  TCTTC                GAAGA(8/7)
MslI          972  CATGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          750  CCGG                 C^CGG
NaeI          749  GCCGGC               GCC^GGC
NgoMIV        749  GCCGGC               G^CCGGC
NlaIV         155  GGGGCC               GG[ACGT]^[ACGT]CC
PflMI         623  CCAAATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         286  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         234  ATTAAT               AT^TAAT
SfcI          824  CTGTAG               C^T[GA][CT]AG
SmlI          221  CTCAAG               C^T[CT][GA]AG
SphI          270  GCATGC               GCATG^C
Tsp45I        422  GTCAC                ^GT[GC]AC
Van91I        623  CCAAATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          815  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          146  GTAC                 GT^AC
              432  GTAC                
BbvI          204  GCTGC                GCAGC(8/12)
              947  GCTGC               
BfaI          163  CTAG                 C^TAG
              208  CTAG                
BsgI          767  GTGCAG               GTGCAG(16/14)
              911  GTGCAG              
BsiYI         623  CCAAATTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              743  CCACTTGCCGG         
BslI          623  CCAAATTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              743  CCACTTGCCGG         
BsmAI         676  GTCTC                GTCTC(1/5)
              848  GTCTC               
BspHI         795  TCATGA               T^CATGA
              930  TCATGA              
Cac8I         270  GCATGC               GC[ACGT]^[ACGT]GC
              749  GCCGGC              
Cfr13I        156  GGGCC                G^G[ACGT]CC
              287  GGTCC               
DpnII         772  GATC                 ^GATC
              907  GATC                
EcoT22I       181  ATGCAT               ATGCA^T
              268  ATGCAT              
Fnu4HI        204  GCTGC                GC^[ACGT]GC
              947  GCTGC               
ItaI          204  GCTGC                GC^[ACGT]GC
              947  GCTGC               
MaeI          163  CTAG                 C^TAG
              208  CTAG                
MboI          772  GATC                 ^GATC
              907  GATC                
MwoI           61  GCTCGGAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              607  GCTTGTGATGC         
NdeII         772  GATC                 ^GATC
              907  GATC                
NsiI          181  ATGCAT               ATGCA^T
              268  ATGCAT              
RcaI          795  TCATGA               T^CATGA
              930  TCATGA              
RsaI          146  GTAC                 GT^AC
              432  GTAC                
Sau3AI        772  GATC                 ^GATC
              907  GATC                
Sau96I        156  GGGCC                G^G[ACGT]CC
              287  GGTCC               
TseI          204  GCTGC                G^C[AT]GC
              947  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BsmFI       BsrI        BssKI       BstNI       
DdeI        EcoRII      HinfI       MaeIII      MnlI        
MseI        MvaI        NlaIII      NspI        PleI        
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
Tsp509I     TspRI       TthHB8I     
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