Restriction map for pF1KB6366
 (Circular) (Six-base) MAPSORT of: pF1KB6366.seq  Check: 6441  from: 1  to: 4007



pF1KB6366  4007 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:    500    1045     500 
   Size:        545    3462
 
Acc65I G'GTAC_C

Cuts at:   1023    1023 
   Size:       4007
 
AclI AA'CG_TT

Cuts at:   3847    3847 
   Size:       4007
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1832    2264    3011    3279    1832 
   Size:        432     747     268    2560

  Fragments arranged by size: 

                2560     747     432     268
 
AflIII A'CryG_T

Cuts at:   1359    2463    3440    1359 
   Size:       1104     977    1926

  Fragments arranged by size: 

                1926    1104     977
 
AgeI A'CCGG_T

Cuts at:   2395    2395 
   Size:       4007
 
AlwNI CAG_nnn'CTG

Cuts at:   1312    2879    1312 
   Size:       1567    2440
 
ApaLI G'TGCA_C

Cuts at:    730    2777     730 
   Size:       2047    1960
 
ApoI r'AATT_y

Cuts at:   1011    3360    3616    1011 
   Size:       2349     256    1402

  Fragments arranged by size: 

                2349    1402     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4007
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4007
 
AvaI C'yCGr_G

Cuts at:   1027    1027 
   Size:       4007
 
BamHI G'GATC_C

Cuts at:   1032    1032 
   Size:       4007
 
BanI G'GyrC_C

Cuts at:    550     925    1023    1437    1685    1720     550 
   Size:        375      98     414     248      35    2837

  Fragments arranged by size: 

                2837     414     375     248      98      35
 
BanII G_rGCy'C

Cuts at:    626    1021    2051     626 
   Size:        395    1030    2582

  Fragments arranged by size: 

                2582    1030     395
 
BbeI G_GCGC'C

Cuts at:    554    1441    1689     554 
   Size:        887     248    2872

  Fragments arranged by size: 

                2872     887     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1905    2666    4007    1905 
   Size:        761    1341    1905

  Fragments arranged by size: 

                1905    1341     761
 
BclI T'GATC_A

Cuts at:    114    1527     114 
   Size:       1413    2594
 
BglI GCCn_nnn'nGGC

Cuts at:    478     478 
   Size:       4007
 
BglII A'GATC_T

Cuts at:    596    1522    3559     596 
   Size:        926    2037    1044

  Fragments arranged by size: 

                2037    1044     926
 
BlpI GC'TnA_GC

Cuts at:   1119    1119 
   Size:       4007
 
Bme1580I G_kGCm'C

Cuts at:    734    1632    1725    2781     734 
   Size:        898      93    1056    1960

  Fragments arranged by size: 

                1960    1056     898      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1219    1300    1635    3779    1219 
   Size:         81     335    2144    1447

  Fragments arranged by size: 

                2144    1447     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2374    2374 
   Size:       4007
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    339     900     339 
   Size:        561    3446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1183    2062    2554    2852    1183 
   Size:        879     492     298    2338

  Fragments arranged by size: 

                2338     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3711    3711 
   Size:       4007
 
BsaAI yAC'GTr

Cuts at:   1360    1360 
   Size:       4007
 
BsaBI GATnn'nnATC

Cuts at:      6    1521    3558       6 
   Size:       1515    2037     455

  Fragments arranged by size: 

                2037    1515     455
 
BsaHI Gr'CG_yC

Cuts at:    155     460     551    1438    1686    2388    3884     155 
   Size:        305      91     887     248     702    1496     278

  Fragments arranged by size: 

                1496     887     702     305     278     248      91
 
BsaWI w'CCGG_w

Cuts at:    463    1196    1717    2395    2669    2816    3227     463 
   Size:        733     521     678     274     147     411    1243

  Fragments arranged by size: 

                1243     733     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    475     557    1432    2190    2767    3263     475 
   Size:         82     875     758     577     496    1219

  Fragments arranged by size: 

                1219     875     758     577     496      82
 
BsiEI CG_ry'CG

Cuts at:    104     185     837    1549    1595    2803     104 
   Size:         81     652     712      46    1208    1308

  Fragments arranged by size: 

                1308    1208     712     652      81      46
 
BsiHKAI G_wGCw'C

Cuts at:    734    1021    1799    1989    2781     734 
   Size:        287     778     190     792    1960

  Fragments arranged by size: 

                1960     792     778     287     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    174     648     174 
   Size:        474    3533
 
Bsp1286I G_dGCh'C

Cuts at:    626     734    1021    1632    1725    1799    1989    2051    2781 
   Size:        108     287     611      93      74     190      62     730

Cuts at:   2781     626 
   Size:       1852

  Fragments arranged by size: 

                1852     730     611     287     190     108      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3227    3227 
   Size:       4007
 
BspHI T'CATG_A

Cuts at:   3183    3183 
   Size:       4007
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    207    1057    1271    1573    1954     207 
   Size:        850     214     302     381    2260

  Fragments arranged by size: 

                2260     850     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2299    2353    3837    4002      50 
   Size:       2249      54    1484     165      55

  Fragments arranged by size: 

                2249    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1919    1919 
   Size:       4007
 
BsrFI r'CCGG_y

Cuts at:   2005    2186    2395    2005 
   Size:        181     209    3617

  Fragments arranged by size: 

                3617     209     181
 
BsrGI T'GTAC_A

Cuts at:    857     857 
   Size:       4007
 
BssSI C'ACGA_G

Cuts at:   2278    2636    2278 
   Size:        358    3649
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1384    1384 
   Size:       4007
 
BstBI TT'CG_AA

Cuts at:   2368    2368 
   Size:       4007
 
BstEII G'GTnAC_C

Cuts at:    325     451     325 
   Size:        126    3881
 
BstYI r'GATC_y

Cuts at:      1     596    1032    1499    1522    1857    2103    3115    3201 
   Size:        595     436     467      23     335     246    1012      86

Cuts at:   3201    3213    3559       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     595     467     449     436     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    293     616     293 
   Size:        323    3684
 
BtgI C'CryG_G

Cuts at:    107     179     570     107 
   Size:         72     391    3544

  Fragments arranged by size: 

                3544     391      72
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1957    2138    2301    1957 
   Size:        181     163    3663

  Fragments arranged by size: 

                3663     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1250    1250 
   Size:       4007
 
DraI TTT'AAA

Cuts at:   1006    1006 
   Size:       4007
 
DrdI GACnn_nn'nnGTC

Cuts at:   1713    2571    1713 
   Size:        858    3149
 
EaeI y'GGCC_r

Cuts at:    146     332     834    1592    1766    2157    2184    3529     146 
   Size:        186     502     758     174     391      27    1345     624

  Fragments arranged by size: 

                1345     758     624     502     391     186     174      27
            
 
EagI C'GGCC_G

Cuts at:    834    1592     834 
   Size:        758    3249
 
EarI CTCTTCn'nnn_

Cuts at:    406    2030    2240     406 
   Size:       1624     210    2173

  Fragments arranged by size: 

                2173    1624     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2215    2525    2671    3817    2215 
   Size:        310     146    1146    2405

  Fragments arranged by size: 

                2405    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1019    1019 
   Size:       4007
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    339     900    1832    2264    3011    3279     339 
   Size:        561     932     432     747     268    1067

  Fragments arranged by size: 

                1067     932     747     561     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    318     318 
   Size:       4007
 
EcoO109I rG'GnC_Cy

Cuts at:   1146    1146 
   Size:       4007
 
EcoRI G'AATT_C

Cuts at:   1011    1011 
   Size:       4007
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    417     933    2026     417 
   Size:        516    1093    2398

  Fragments arranged by size: 

                2398    1093     516
 
FspI TGC'GCA

Cuts at:   1788    1788 
   Size:       4007
 
HaeII r_GCGC'y

Cuts at:    441     554    1441    1689    2711    3690     441 
   Size:        113     887     248    1022     979     758

  Fragments arranged by size: 

                1022     979     887     758     248     113
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1849    1879    1881    1911    1849 
   Size:         30       2      30    3945

  Fragments arranged by size: 

                3945      30      30       2
 
HincII GTy'rAC

Cuts at:    873    1046     873 
   Size:        173    3834
 
KasI G'GCGC_C

Cuts at:    550    1437    1685     550 
   Size:        887     248    2872

  Fragments arranged by size: 

                2872     887     248
 
KpnI G_GTAC'C

Cuts at:   1027    1027 
   Size:       4007
 
MluI A'CGCG_T

Cuts at:   3440    3440 
   Size:       4007
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2678    2862    2678 
   Size:        184    3823
 
MscI TGG'CCA

Cuts at:    334    1768     334 
   Size:       1434    2573
 
MslI CAynn'nnrTG

Cuts at:    739    1996    2123     739 
   Size:       1257     127    2623

  Fragments arranged by size: 

                2623    1257     127
 
MspA1I CmG'CkG

Cuts at:    163     181     475     507    1118    1432    1792    2805    3050 
   Size:         18     294      32     611     314     360    1013     245

Cuts at:   3050     163 
   Size:       1120

  Fragments arranged by size: 

                1120    1013     611     360     314     294     245      32
                  18
 
NaeI GCC'GGC

Cuts at:   2188    2188 
   Size:       4007
 
NarI GG'CG_CC

Cuts at:    551    1438    1686     551 
   Size:        887     248    2872

  Fragments arranged by size: 

                2872     887     248
 
NcoI C'CATG_G

Cuts at:    107     570     107 
   Size:        463    3544
 
NgoMIV G'CCGG_C

Cuts at:   2186    2186 
   Size:       4007
 
NspI r_CATG'y

Cuts at:    229    1060    1560    2467     229 
   Size:        831     500     907    1769

  Fragments arranged by size: 

                1769     907     831     500
 
PciI A'CATG_T

Cuts at:   2463    2463 
   Size:       4007
 
PflMI CCAn_nnn'nTGG

Cuts at:    576     576 
   Size:       4007
 
PmeI GTTT'AAAC

Cuts at:   1006    1006 
   Size:       4007
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3146    3178    3146 
   Size:         32    3975
 
PshAI GACnn'nnGTC

Cuts at:    181     772     181 
   Size:        591    3416
 
PstI C_TGCA'G

Cuts at:    218    1054    1739     218 
   Size:        836     685    2486

  Fragments arranged by size: 

                2486     836     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4007
 
PvuII CAG'CTG

Cuts at:   1432    1792    1432 
   Size:        360    3647
 
RsrII CG'GwC_CG

Cuts at:   2202    2202 
   Size:       4007
 
SacI G_AGCT'C

Cuts at:   1021    1021 
   Size:       4007
 
SacII CC_GC'GG

Cuts at:    182     182 
   Size:       4007
 
SalI G'TCGA_C

Cuts at:   1044    1044 
   Size:       4007
 
SapI GCTCTTCn'nnn_

Cuts at:    406    2030    2240     406 
   Size:       1624     210    2173

  Fragments arranged by size: 

                2173    1624     210
 
SbfI CC_TGCA'GG

Cuts at:   1054    1054 
   Size:       4007
 
ScaI AGT'ACT

Cuts at:   3524    3524 
   Size:       4007
 
SexAI A'CCwGG_T

Cuts at:    523     523 
   Size:       4007
 
SfcI C'TryA_G

Cuts at:     33     214     666    1050    1735    2728    2919      33 
   Size:        181     452     384     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     452     384     191     181
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    478     478 
   Size:       4007
 
SfoI GGC'GCC

Cuts at:    552    1439    1687     552 
   Size:        887     248    2872

  Fragments arranged by size: 

                2872     887     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4007
 
SmaI CCC'GGG

Cuts at:   1029    1029 
   Size:       4007
 
SmlI C'TyrA_G

Cuts at:   1162    2077    2569    2831    1162 
   Size:        915     492     262    2338

  Fragments arranged by size: 

                2338     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4007
 
SphI G_CATG'C

Cuts at:    229    1060    1560     229 
   Size:        831     500    2676

  Fragments arranged by size: 

                2676     831     500
 
StuI AGG'CCT

Cuts at:    913     913 
   Size:       4007
 
StyI C'CwwG_G

Cuts at:    107     570     651     701     908    1141    1495     107 
   Size:        463      81      50     207     233     354    2619

  Fragments arranged by size: 

                2619     463     354     233     207      81      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    100     373    1590    1723    2182    2391     100 
   Size:        273    1217     133     459     209    1716

  Fragments arranged by size: 

                1716    1217     459     273     209     133
 
TatI w'GTAC_w

Cuts at:    257     389     857    3522     257 
   Size:        132     468    2665     742

  Fragments arranged by size: 

                2665     742     468     132
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3524    3556    3524 
   Size:         32    3975
 
Tth111I GACn'n_nGTC

Cuts at:   1804    1804 
   Size:       4007
 
XbaI T'CTAG_A

Cuts at:   1038    1038 
   Size:       4007
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1502    1502 
   Size:       4007
 
XmaI C'CCGG_G

Cuts at:   1027    1027 
   Size:       4007
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PmeI     PpiI    PshAI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
    BmgBI     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
   BssHII    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmlI    PpuMI     PsiI   PspOMI
    PspXI     PsrI    SanDI    SgrAI    SnaBI     SrfI     SspI     SwaI
     XhoI     XmnI     ZraI
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