Restriction map for pF1KB6366
 (Linear) (Six-base) MAPSORT of: KIBB6366.seq  Check: 1827  from: 1  to: 894



KIBB6366.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:      0     392     894 
   Size:        392     502
 
ApaLI G'TGCA_C

Cuts at:      0     622     894 
   Size:        622     272
 
BanI G'GyrC_C

Cuts at:      0     442     817     894 
   Size:        442     375      77

  Fragments arranged by size: 

                 442     375      77
 
BanII G_rGCy'C

Cuts at:      0     518     894 
   Size:        518     376
 
BbeI G_GCGC'C

Cuts at:      0     446     894 
   Size:        446     448
 
BclI T'GATC_A

Cuts at:      0       6     894 
   Size:          6     888
 
BglI GCCn_nnn'nGGC

Cuts at:      0     370     894 
   Size:        370     524
 
BglII A'GATC_T

Cuts at:      0     488     894 
   Size:        488     406
 
Bme1580I G_kGCm'C

Cuts at:      0     626     894 
   Size:        626     268
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     792     894 
   Size:        231     561     102

  Fragments arranged by size: 

                 561     231     102
 
BsaHI Gr'CG_yC

Cuts at:      0      47     352     443     894 
   Size:         47     305      91     451

  Fragments arranged by size: 

                 451     305      91      47
 
BsaWI w'CCGG_w

Cuts at:      0     355     894 
   Size:        355     539
 
BseYI C'CCAG_C

Cuts at:      0     367     449     894 
   Size:        367      82     445

  Fragments arranged by size: 

                 445     367      82
 
BsiEI CG_ry'CG

Cuts at:      0      77     729     894 
   Size:         77     652     165

  Fragments arranged by size: 

                 652     165      77
 
BsiHKAI G_wGCw'C

Cuts at:      0     626     894 
   Size:        626     268
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      66     540     894 
   Size:         66     474     354

  Fragments arranged by size: 

                 474     354      66
 
Bsp1286I G_dGCh'C

Cuts at:      0     518     626     894 
   Size:        518     108     268

  Fragments arranged by size: 

                 518     268     108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      99     894 
   Size:         99     795
 
BsrGI T'GTAC_A

Cuts at:      0     749     894 
   Size:        749     145
 
BstEII G'GTnAC_C

Cuts at:      0     217     343     894 
   Size:        217     126     551

  Fragments arranged by size: 

                 551     217     126
 
BstYI r'GATC_y

Cuts at:      0     488     894 
   Size:        488     406
 
Bsu36I CC'TnA_GG

Cuts at:      0     185     508     894 
   Size:        185     323     386

  Fragments arranged by size: 

                 386     323     185
 
BtgI C'CryG_G

Cuts at:      0      71     462     894 
   Size:         71     391     432

  Fragments arranged by size: 

                 432     391      71
 
EaeI y'GGCC_r

Cuts at:      0      38     224     726     894 
   Size:         38     186     502     168

  Fragments arranged by size: 

                 502     186     168      38
 
EagI C'GGCC_G

Cuts at:      0     726     894 
   Size:        726     168
 
EarI CTCTTCn'nnn_

Cuts at:      0     298     894 
   Size:        298     596
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     792     894 
   Size:        231     561     102

  Fragments arranged by size: 

                 561     231     102
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     210     894 
   Size:        210     684
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     309     825     894 
   Size:        309     516      69

  Fragments arranged by size: 

                 516     309      69
 
HaeII r_GCGC'y

Cuts at:      0     333     446     894 
   Size:        333     113     448

  Fragments arranged by size: 

                 448     333     113
 
HincII GTy'rAC

Cuts at:      0     765     894 
   Size:        765     129
 
KasI G'GCGC_C

Cuts at:      0     442     894 
   Size:        442     452
 
MscI TGG'CCA

Cuts at:      0     226     894 
   Size:        226     668
 
MslI CAynn'nnrTG

Cuts at:      0     631     894 
   Size:        631     263
 
MspA1I CmG'CkG

Cuts at:      0      55      73     367     399     894 
   Size:         55      18     294      32     495

  Fragments arranged by size: 

                 495     294      55      32      18
 
NarI GG'CG_CC

Cuts at:      0     443     894 
   Size:        443     451
 
NcoI C'CATG_G

Cuts at:      0     462     894 
   Size:        462     432
 
NspI r_CATG'y

Cuts at:      0     121     894 
   Size:        121     773
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     468     894 
   Size:        468     426
 
PshAI GACnn'nnGTC

Cuts at:      0      73     664     894 
   Size:         73     591     230

  Fragments arranged by size: 

                 591     230      73
 
PstI C_TGCA'G

Cuts at:      0     110     894 
   Size:        110     784
 
SacII CC_GC'GG

Cuts at:      0      74     894 
   Size:         74     820
 
SapI GCTCTTCn'nnn_

Cuts at:      0     298     894 
   Size:        298     596
 
SexAI A'CCwGG_T

Cuts at:      0     415     894 
   Size:        415     479
 
SfcI C'TryA_G

Cuts at:      0     106     558     894 
   Size:        106     452     336

  Fragments arranged by size: 

                 452     336     106
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     370     894 
   Size:        370     524
 
SfoI GGC'GCC

Cuts at:      0     444     894 
   Size:        444     450
 
SphI G_CATG'C

Cuts at:      0     121     894 
   Size:        121     773
 
StuI AGG'CCT

Cuts at:      0     805     894 
   Size:        805      89
 
StyI C'CwwG_G

Cuts at:      0     462     543     593     800     894 
   Size:        462      81      50     207      94

  Fragments arranged by size: 

                 462     207      94      81      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     265     894 
   Size:        265     629
 
TatI w'GTAC_w

Cuts at:      0     149     281     749     894 
   Size:        149     132     468     145

  Fragments arranged by size: 

                 468     149     145     132
 

 Enzymes that do cut:

     AccI    ApaLI     BanI    BanII     BbeI     BclI     BglI    BglII
 Bme1580I     BpmI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrGI   BstEII    BstYI   Bsu36I     BtgI     EaeI
     EagI     EarI  Eco57MI    EcoNI     FalI    HaeII   HincII     KasI
     MscI     MslI   MspA1I     NarI     NcoI     NspI    PflMI    PshAI
     PstI    SacII     SapI    SexAI     SfcI     SfiI     SfoI     SphI
     StuI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI
     BsgI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SbfI     ScaI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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