Restriction map for
pF1KB6524
(Circular) (Six-base) MAPSORT of: pF1KB6524.seq Check: 9375 from: 1 to: 4274
pF1KB6524 4274 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 573 573
Size: 4274
AccI GT'mk_AC
Cuts at: 695 1312 695
Size: 617 3657
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 757 1087 2099 2531 3278 3546 601
Size: 156 330 1012 432 747 268 1329
Fragments arranged by size:
1329 1012 747 432 330 268 156
AflIII A'CryG_T
Cuts at: 1626 2730 3707 1626
Size: 1104 977 2193
Fragments arranged by size:
2193 1104 977
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AleI CACnn'nnGTG
Cuts at: 1208 1208
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 465 1579 3146 465
Size: 1114 1567 1593
Fragments arranged by size:
1593 1567 1114
ApaLI G'TGCA_C
Cuts at: 217 1201 3044 217
Size: 984 1843 1447
Fragments arranged by size:
1843 1447 984
ApoI r'AATT_y
Cuts at: 1278 3627 3883 1278
Size: 2349 256 1669
Fragments arranged by size:
2349 1669 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 1294 1294
Size: 4274
BamHI G'GATC_C
Cuts at: 908 1299 908
Size: 391 3883
BanI G'GyrC_C
Cuts at: 816 888 993 1164 1245 1290 1704 1952 1987
Size: 72 105 171 81 45 414 248 35
Cuts at: 1987 816
Size: 3103
Fragments arranged by size:
3103 414 248 171 105 81 72 45
35
BanII G_rGCy'C
Cuts at: 407 608 1288 2318 407
Size: 201 680 1030 2363
Fragments arranged by size:
2363 1030 680 201
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4026
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2933 4274 2172
Size: 761 1341 2172
Fragments arranged by size:
2172 1341 761
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BglI GCCn_nnn'nGGC
Cuts at: 330 330
Size: 4274
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 1386 1386
Size: 4274
Bme1580I G_kGCm'C
Cuts at: 221 777 893 998 1169 1205 1250 1899 1992
Size: 556 116 105 171 36 45 649 93
Cuts at: 1992 3048 221
Size: 1056 1447
Fragments arranged by size:
1447 1056 649 556 171 116 105 93
45 36
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1567 1902 4046 1486
Size: 81 335 2144 1714
Fragments arranged by size:
2144 1714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 391 493 934 289
Size: 102 102 441 3629
Fragments arranged by size:
3629 441 102 102
Bpu10I CC'TnA_GC
Cuts at: 254 254
Size: 4274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2329 2821 3119 1450
Size: 879 492 298 2605
Fragments arranged by size:
2605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1067 3978 1067
Size: 2911 1363
BsaAI yAC'GTr
Cuts at: 1627 1627
Size: 4274
BsaBI GATnn'nnATC
Cuts at: 6 1788 3825 6
Size: 1782 2037 455
Fragments arranged by size:
2037 1782 455
BsaHI Gr'CG_yC
Cuts at: 1705 1953 2655 4151 1705
Size: 248 702 1496 1828
Fragments arranged by size:
1828 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1463 1984 2662 2936 3083 3494 1463
Size: 521 678 274 147 411 2243
Fragments arranged by size:
2243 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 122 127 559 1147 122
Size: 5 432 588 3249
Fragments arranged by size:
3249 588 432 5
BseYI C'CCAG_C
Cuts at: 159 176 344 1699 2457 3034 3530 159
Size: 17 168 1355 758 577 496 903
Fragments arranged by size:
1355 903 758 577 496 168 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1095
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3070 104
Size: 1712 46 1208 1308
Fragments arranged by size:
1712 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 1205 1288 2066 2256 3048 221
Size: 984 83 778 190 792 1447
Fragments arranged by size:
1447 984 792 778 190 83
BsmBI CGTCTCn'nnnn_
Cuts at: 229 229
Size: 4274
Bsp1286I G_dGCh'C
Cuts at: 221 407 608 777 893 998 1169 1205 1250
Size: 186 201 169 116 105 171 36 45
Cuts at: 1250 1288 1899 1992 2066 2256 2318 3048 221
Size: 38 611 93 74 190 62 730 1447
Fragments arranged by size:
1447 730 611 201 190 186 171 169
116 105 93 74 62 45 38 36
BspEI T'CCGG_A
Cuts at: 3494 3494
Size: 4274
BspHI T'CATG_A
Cuts at: 3450 3450
Size: 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 329 573 1324 1538 1840 2221 329
Size: 244 751 214 302 381 2382
Fragments arranged by size:
2382 751 381 302 244 214
BsrBI CCG'CTC
Cuts at: 50 1215 2566 2620 4104 4269 50
Size: 1165 1351 54 1484 165 55
Fragments arranged by size:
1484 1351 1165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 849 2186 849
Size: 1337 2937
BsrFI r'CCGG_y
Cuts at: 1049 2272 2453 2662 1049
Size: 1223 181 209 2661
Fragments arranged by size:
2661 1223 209 181
BssSI C'ACGA_G
Cuts at: 139 2545 2903 139
Size: 2406 358 1510
Fragments arranged by size:
2406 1510 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1651 1651
Size: 4274
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstEII G'GTnAC_C
Cuts at: 981 981
Size: 4274
BstXI CCAn_nnnn'nTGG
Cuts at: 364 364
Size: 4274
BstYI r'GATC_y
Cuts at: 1 908 1299 1766 1789 2124 2370 3382 3468
Size: 907 391 467 23 335 246 1012 86
Cuts at: 3468 3480 3826 1
Size: 12 346 449
Fragments arranged by size:
1012 907 467 449 391 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 696 696
Size: 4274
BtgI C'CryG_G
Cuts at: 107 648 1119 107
Size: 541 471 3262
Fragments arranged by size:
3262 541 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 1107 1517 1107
Size: 410 3864
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 1980 2838 1980
Size: 858 3416
EaeI y'GGCC_r
Cuts at: 1151 1171 1859 2033 2424 2451 3796 1151
Size: 20 688 174 391 27 1345 1629
Fragments arranged by size:
1629 1345 688 391 174 27 20
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4274
EarI CTCTTCn'nnn_
Cuts at: 127 1063 2297 2507 127
Size: 936 1234 210 1894
Fragments arranged by size:
1894 1234 936 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2482 2792 2938 4084 2482
Size: 310 146 1146 2672
Fragments arranged by size:
2672 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1286 1286
Size: 4274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 391 493 601 757 934 1087 2099 2531
Size: 102 102 108 156 177 153 1012 432
Cuts at: 2531 3278 3546 289
Size: 747 268 1017
Fragments arranged by size:
1017 1012 747 432 268 177 156 153
108 102 102
EcoO109I rG'GnC_Cy
Cuts at: 483 493 948 1413 483
Size: 10 455 465 3344
Fragments arranged by size:
3344 465 455 10
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 4274
FspI TGC'GCA
Cuts at: 529 2055 529
Size: 1526 2748
HaeII r_GCGC'y
Cuts at: 1241 1708 1956 2978 3957 1241
Size: 467 248 1022 979 1558
Fragments arranged by size:
1558 1022 979 467 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2116 2146 2148 2178 2116
Size: 30 2 30 4212
Fragments arranged by size:
4212 30 30 2
HincII GTy'rAC
Cuts at: 834 1313 834
Size: 479 3795
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4026
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 2945 3129 1112
Size: 1833 184 2257
Fragments arranged by size:
2257 1833 184
MscI TGG'CCA
Cuts at: 2035 2035
Size: 4274
MslI CAynn'nnrTG
Cuts at: 703 1096 1208 2263 2390 703
Size: 393 112 1055 127 2587
Fragments arranged by size:
2587 1055 393 127 112
MspA1I CmG'CkG
Cuts at: 277 621 770 1385 1699 2059 3072 3317 277
Size: 344 149 615 314 360 1013 245 1234
Fragments arranged by size:
1234 1013 615 360 344 314 245 149
NaeI GCC'GGC
Cuts at: 1051 2455 1051
Size: 1404 2870
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4026
NcoI C'CATG_G
Cuts at: 107 107
Size: 4274
NgoMIV G'CCGG_C
Cuts at: 1049 2453 1049
Size: 1404 2870
NspI r_CATG'y
Cuts at: 262 1327 1827 2734 262
Size: 1065 500 907 1802
Fragments arranged by size:
1802 1065 907 500
PasI CC'CwG_GG
Cuts at: 777 777
Size: 4274
PciI A'CATG_T
Cuts at: 2730 2730
Size: 4274
PflMI CCAn_nnn'nTGG
Cuts at: 904 904
Size: 4274
PfoI T'CCnGG_A
Cuts at: 911 911
Size: 4274
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PpuMI rG'GwC_Cy
Cuts at: 483 483
Size: 4274
PstI C_TGCA'G
Cuts at: 882 1321 2006 882
Size: 439 685 3150
Fragments arranged by size:
3150 685 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 621 1699 2059 621
Size: 1078 360 2836
Fragments arranged by size:
2836 1078 360
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 1288 1288
Size: 4274
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SanDI GG'GwC_CC
Cuts at: 483 483
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 3791 3791
Size: 4274
SfcI C'TryA_G
Cuts at: 33 878 1317 2002 2995 3186 33
Size: 845 439 685 993 191 1121
Fragments arranged by size:
1121 993 845 685 439 191
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4026
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4274
SmlI C'TyrA_G
Cuts at: 1429 2344 2836 3098 1429
Size: 915 492 262 2605
Fragments arranged by size:
2605 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 1327 1827 1327
Size: 500 3774
StyI C'CwwG_G
Cuts at: 107 224 587 641 812 1408 1762 107
Size: 117 363 54 171 596 354 2619
Fragments arranged by size:
2619 596 363 354 171 117 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 329 358 954 1857 1990 2449 2658 209
Size: 120 29 596 903 133 459 209 1825
Fragments arranged by size:
1825 903 596 459 209 133 120 29
TatI w'GTAC_w
Cuts at: 3789 3789
Size: 4274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 263 295 809 841 3791 3823 263
Size: 32 514 32 2950 32 714
Fragments arranged by size:
2950 714 514 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2071 2071
Size: 4274
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1769 1769
Size: 4274
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4274
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsrGI BssHII Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI