Restriction map for pF1KE0211
##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
MvaI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AluI           29  AGCT                 AG^CT
AspHI          34  GAGCAC               G[AT]GC[AT]^C
AvaI          247  CCCGAG               C^[CT]CG[GA]G
AvaII         229  GGACC                G^G[AT]CC
BmyI           34  GAGCAC               G[AGT]GC[ACT]^C
BpmI           31  CTGGAG               CTGGAG(16/14)
BsaJI         248  CCGAGG               C^C[ACGT][ACGT]GG
BseRI         250  GAGGAG               GAGGAG(10/8)
BsiHKAI        34  GAGCAC               G[AT]GC[AT]^C
BsiYI          77  CCACCAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           77  CCACCAAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         247  CCCGAG               C^[CT]CG[GA]G
Bsp1286I       34  GAGCAC               G[AGT]GC[ACT]^C
BspMI          38  ACCTGC               ACCTGC(4/8)
BsrDI         162  CATTGC               GCAATG(2/0)
BstF5I         18  CATCC                GGATG(2/0)
CfoI          184  GCGC                 GCG^C
DraIII         78  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
FokI           18  CATCC                GGATG(9/13)
HhaI          184  GCGC                 GCG^C
HinP1I        184  GCGC                 G^CGC
HphI           96  GGTGA                GGTGA(8/7)
MnlI          250  GAGG                 CCTC(7/6)
MspA1I         28  CAGCTG               C[AC]G^C[GT]G
NlaIII        129  CATG                 CATG^
PflMI          77  CCACCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           40  CTGCAG               CTGCA^G
PvuII          28  CAGCTG               CAG^CTG
SfaNI         213  GATGC                GCATC(5/9)
SfcI           40  CTGCAG               C^T[GA][CT]AG
TaqI          140  TCGA                 T^CGA
TthHB8I       140  TCGA                 T^CGA
Van91I         77  CCACCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AccII         109  CGCG                 CG^CG
              185  CGCG                
AfaI           51  GTAC                 GT^AC
              117  GTAC                
BstUI         109  CGCG                 CG^CG
              185  CGCG                
Cac8I          26  GCCAGC               GC[ACGT]^[ACGT]GC
              234  GCCAGC              
HaeIII         64  GGCC                 GG^CC
              133  GGCC                
HinfI         112  GACTC                G^A[ACGT]TC
              188  GAGTC               
MvnI          109  CGCG                 CG^CG
              185  CGCG                
PleI          112  GACTC                GAGTC(4/5)
              188  GAGTC               
RsaI           51  GTAC                 GT^AC
              117  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AciI        Cfr13I      Sau96I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com