Restriction map for pF1KB7331
 (Linear) (Six-base) MAPSORT of: KIBB7331.seq  Check: 9720  from: 1  to: 2982



KIBB7331.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     303    1376    2443    2751    2982 
   Size:        303    1073    1067     308     231

  Fragments arranged by size: 

                1073    1067     308     303     231
 
AccI GT'mk_AC

Cuts at:      0     830    2831    2982 
   Size:        830    2001     151

  Fragments arranged by size: 

                2001     830     151
 
Acc65I G'GTAC_C

Cuts at:      0     384    1654    2982 
   Size:        384    1270    1328

  Fragments arranged by size: 

                1328    1270     384
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     635    1186    1608    2635    2982 
   Size:        635     551     422    1027     347

  Fragments arranged by size: 

                1027     635     551     422     347
 
AflIII A'CryG_T

Cuts at:      0    2125    2982 
   Size:       2125     857
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     502    1579    2982 
   Size:        502    1077    1403

  Fragments arranged by size: 

                1403    1077     502
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178    2982 
   Size:        146      32    2804

  Fragments arranged by size: 

                2804     146      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     539     895    2982 
   Size:        539     356    2087

  Fragments arranged by size: 

                2087     539     356
 
ApaI G_GGCC'C

Cuts at:      0     476    1994    2481    2982 
   Size:        476    1518     487     501

  Fragments arranged by size: 

                1518     501     487     476
 
ApaLI G'TGCA_C

Cuts at:      0    2185    2982 
   Size:       2185     797
 
ApoI r'AATT_y

Cuts at:      0     223     738    2982 
   Size:        223     515    2244

  Fragments arranged by size: 

                2244     515     223
 
AvaI C'yCGr_G

Cuts at:      0    1299    1967    2056    2982 
   Size:       1299     668      89     926

  Fragments arranged by size: 

                1299     926     668      89
 
AvrII C'CTAG_G

Cuts at:      0     495    2982 
   Size:        495    2487
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1646    1679    2982 
   Size:       1646      33    1303

  Fragments arranged by size: 

                1646    1303      33
 
BamHI G'GATC_C

Cuts at:      0     620    2236    2982 
   Size:        620    1616     746

  Fragments arranged by size: 

                1616     746     620
 
BanI G'GyrC_C

Cuts at:      0     384    1030    1171    1654    2109    2191    2539    2648 
   Size:        384     646     141     483     455      82     348     109

Cuts at:   2648    2739    2982 
   Size:         91     243

  Fragments arranged by size: 

                 646     483     455     384     348     243     141     109
                  91      82
 
BanII G_rGCy'C

Cuts at:      0     476     760    1229    1526    1604    1705    1835    1994 
   Size:        476     284     469     297      78     101     130     159

Cuts at:   1994    2167    2481    2982 
   Size:        173     314     501

  Fragments arranged by size: 

                 501     476     469     314     297     284     173     159
                 130     101      78
 
BbeI G_GCGC'C

Cuts at:      0    1175    2982 
   Size:       1175    1807
 
BbvCI CC'TCA_GC

Cuts at:      0     262     781    2982 
   Size:        262     519    2201

  Fragments arranged by size: 

                2201     519     262
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1757    1791    2982 
   Size:       1757      34    1191

  Fragments arranged by size: 

                1757    1191      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     755    2453    2982 
   Size:        755    1698     529

  Fragments arranged by size: 

                1698     755     529
 
BlpI GC'TnA_GC

Cuts at:      0    1495    2982 
   Size:       1495    1487
 
Bme1580I G_kGCm'C

Cuts at:      0     476     491    1137    1249    1994    2114    2189    2481 
   Size:        476      15     646     112     745     120      75     292

Cuts at:   2481    2529    2544    2601    2651    2982 
   Size:         48      15      57      50     331

  Fragments arranged by size: 

                 745     646     476     331     292     120     112      75
                  57      50      48      15      15
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     393    1734    2982 
   Size:        393    1341    1248

  Fragments arranged by size: 

                1341    1248     393
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1719    2982 
   Size:       1719    1263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     438     452     584    1274    1714    2342    2736 
   Size:        353      85      14     132     690     440     628     394

Cuts at:   2736    2829    2836    2982 
   Size:         93       7     146

  Fragments arranged by size: 

                 690     628     440     394     353     146     132      93
                  85      14       7
 
Bpu10I CC'TnA_GC

Cuts at:      0     143     262     781    1063    2177    2982 
   Size:        143     119     519     282    1114     805

  Fragments arranged by size: 

                1114     805     519     282     143     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238    1360    2488    2982 
   Size:        238    1122    1128     494

  Fragments arranged by size: 

                1128    1122     494     238
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     661    2524    2982 
   Size:        661    1863     458

  Fragments arranged by size: 

                1863     661     458
 
BsaBI GATnn'nnATC

Cuts at:      0     619    2982 
   Size:        619    2363
 
BsaHI Gr'CG_yC

Cuts at:      0     161    1172    2343    2982 
   Size:        161    1011    1171     639

  Fragments arranged by size: 

                1171    1011     639     161
 
BsaWI w'CCGG_w

Cuts at:      0     423    2130    2982 
   Size:        423    1707     852

  Fragments arranged by size: 

                1707     852     423
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     418     448     769     799    2982 
   Size:        418      30     321      30    2183

  Fragments arranged by size: 

                2183     418     321      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     271     630     679    2982 
   Size:        121     150     359      49    2303

  Fragments arranged by size: 

                2303     359     150     121      49
 
BseYI C'CCAG_C

Cuts at:      0     475     529    1202    2070    2568    2982 
   Size:        475      54     673     868     498     414

  Fragments arranged by size: 

                 868     673     498     475     414      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1354    1797    2982 
   Size:        281    1073     443    1185

  Fragments arranged by size: 

                1185    1073     443     281
 
BsiEI CG_ry'CG

Cuts at:      0    1278    1842    2982 
   Size:       1278     564    1140

  Fragments arranged by size: 

                1278    1140     564
 
BsiHKAI G_wGCw'C

Cuts at:      0    1526    2189    2336    2982 
   Size:       1526     663     147     646

  Fragments arranged by size: 

                1526     663     646     147
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1290    2963    2982 
   Size:       1290    1673      19

  Fragments arranged by size: 

                1673    1290      19
 
Bsp1286I G_dGCh'C

Cuts at:      0     476     491     760    1137    1229    1249    1526    1604 
   Size:        476      15     269     377      92      20     277      78

Cuts at:   1604    1705    1835    1994    2114    2167    2189    2336    2481 
   Size:        101     130     159     120      53      22     147     145

Cuts at:   2481    2529    2544    2601    2651    2982 
   Size:         48      15      57      50     331

  Fragments arranged by size: 

                 476     377     331     277     269     159     147     145
                 130     120     101      92      78      57      53      50
                  48      22      20      15      15
 
BspEI T'CCGG_A

Cuts at:      0     423    2130    2982 
   Size:        423    1707     852

  Fragments arranged by size: 

                1707     852     423
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     303    1376    1484    2291    2443    2751    2982 
   Size:        303    1073     108     807     152     308     231

  Fragments arranged by size: 

                1073     807     308     303     231     152     108
 
BsrDI GCAATG_nn'

Cuts at:      0    1269    1428    1590    2124    2774    2982 
   Size:       1269     159     162     534     650     208

  Fragments arranged by size: 

                1269     650     534     208     162     159
 
BsrFI r'CCGG_y

Cuts at:      0    1017    1273    2982 
   Size:       1017     256    1709

  Fragments arranged by size: 

                1709    1017     256
 
BssSI C'ACGA_G

Cuts at:      0    2592    2982 
   Size:       2592     390
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2746    2982 
   Size:       2746     236
 
BstBI TT'CG_AA

Cuts at:      0     447    2982 
   Size:        447    2535
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2320    2760    2982 
   Size:       2320     440     222

  Fragments arranged by size: 

                2320     440     222
 
BstYI r'GATC_y

Cuts at:      0     620    2144    2236    2950    2982 
   Size:        620    1524      92     714      32

  Fragments arranged by size: 

                1524     714     620      92      32
 
BstZ17I GTA'TAC

Cuts at:      0    2832    2982 
   Size:       2832     150
 
Bsu36I CC'TnA_GG

Cuts at:      0     994    1349    2982 
   Size:        994     355    1633

  Fragments arranged by size: 

                1633     994     355
 
BtgI C'CryG_G

Cuts at:      0      77     374     580    2021    2195    2369    2514    2982 
   Size:         77     297     206    1441     174     174     145     468

  Fragments arranged by size: 

                1441     468     297     206     174     174     145      77
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2153    2821    2982 
   Size:       2153     668     161

  Fragments arranged by size: 

                2153     668     161
 
BtsI GCAGTG_nn'

Cuts at:      0     271     491     922    2982 
   Size:        271     220     431    2060

  Fragments arranged by size: 

                2060     431     271     220
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     780     815    2982 
   Size:        780      35    2167

  Fragments arranged by size: 

                2167     780      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     419    2982 
   Size:        419    2563
 
EaeI y'GGCC_r

Cuts at:      0     329     932    1275    1825    2982 
   Size:        329     603     343     550    1157

  Fragments arranged by size: 

                1157     603     550     343     329
 
EagI C'GGCC_G

Cuts at:      0    1275    2982 
   Size:       1275    1707
 
EarI CTCTTCn'nnn_

Cuts at:      0    1871    2155    2982 
   Size:       1871     284     827

  Fragments arranged by size: 

                1871     827     284
 
EcoICRI GAG'CTC

Cuts at:      0    1524    2982 
   Size:       1524    1458
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     438     452     584     635    1186    1274    1608 
   Size:        353      85      14     132      51     551      88     334

Cuts at:   1608    1714    2342    2635    2736    2829    2836    2982 
   Size:        106     628     293     101      93       7     146

  Fragments arranged by size: 

                 628     551     353     334     293     146     132     106
                 101      93      88      85      51      14       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     232    1393    1696    2982 
   Size:        232    1161     303    1286

  Fragments arranged by size: 

                1286    1161     303     232
 
EcoO109I rG'GnC_Cy

Cuts at:      0      69     412     472     559     724     913    1440    1488 
   Size:         69     343      60      87     165     189     527      48

Cuts at:   1488    1921    1990    1991    2060    2262    2280    2478    2982 
   Size:        433      69       1      69     202      18     198     504

  Fragments arranged by size: 

                 527     504     433     343     202     198     189     165
                  87      69      69      69      60      48      18       1
            
 
EcoRI G'AATT_C

Cuts at:      0     223    2982 
   Size:        223    2759
 
FspI TGC'GCA

Cuts at:      0    1915    2982 
   Size:       1915    1067
 
HaeII r_GCGC'y

Cuts at:      0      43    1175    2982 
   Size:         43    1132    1807

  Fragments arranged by size: 

                1807    1132      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     418     450     583     615     769     801    2228    2260 
   Size:        418      32     133      32     154      32    1427      32

Cuts at:   2260    2272    2304    2935    2937    2967    2969    2982 
   Size:         12      32     631       2      30       2      13

  Fragments arranged by size: 

                1427     631     418     154     133      32      32      32
                  32      32      30      13      12       2       2
 
HincII GTy'rAC

Cuts at:      0    1725    2982 
   Size:       1725    1257
 
KasI G'GCGC_C

Cuts at:      0    1171    2982 
   Size:       1171    1811
 
KpnI G_GTAC'C

Cuts at:      0     388    1658    2982 
   Size:        388    1270    1324

  Fragments arranged by size: 

                1324    1270     388
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2030    2959    2982 
   Size:       2030     929      23

  Fragments arranged by size: 

                2030     929      23
 
MscI TGG'CCA

Cuts at:      0     331     934    1827    2982 
   Size:        331     603     893    1155

  Fragments arranged by size: 

                1155     893     603     331
 
MslI CAynn'nnrTG

Cuts at:      0     484    1595    1906    2663    2982 
   Size:        484    1111     311     757     319

  Fragments arranged by size: 

                1111     757     484     319     311
 
MspA1I CmG'CkG

Cuts at:      0     213    1164    1359    1541    1549    1673    1781    2782 
   Size:        213     951     195     182       8     124     108    1001

Cuts at:   2782    2874    2982 
   Size:         92     108

  Fragments arranged by size: 

                1001     951     213     195     182     124     108     108
                  92       8
 
NarI GG'CG_CC

Cuts at:      0    1172    2982 
   Size:       1172    1810
 
NcoI C'CATG_G

Cuts at:      0     374    2021    2514    2982 
   Size:        374    1647     493     468

  Fragments arranged by size: 

                1647     493     468     374
 
NspI r_CATG'y

Cuts at:      0    1873    2129    2982 
   Size:       1873     256     853

  Fragments arranged by size: 

                1873     853     256
 
PasI CC'CwG_GG

Cuts at:      0     502     603    1123    1249    2982 
   Size:        502     101     520     126    1733

  Fragments arranged by size: 

                1733     520     502     126     101
 
PciI A'CATG_T

Cuts at:      0    2125    2982 
   Size:       2125     857
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1021    1093    2156    2232    2515    2982 
   Size:       1021      72    1063      76     283     467

  Fragments arranged by size: 

                1063    1021     467     283      76      72
 
PfoI T'CCnGG_A

Cuts at:      0     561     875    2319    2492    2982 
   Size:        561     314    1444     173     490

  Fragments arranged by size: 

                1444     561     490     314     173
 
PpuMI rG'GwC_Cy

Cuts at:      0     412     559    2060    2262    2280    2982 
   Size:        412     147    1501     202      18     702

  Fragments arranged by size: 

                1501     702     412     202     147      18
 
PshAI GACnn'nnGTC

Cuts at:      0    2126    2982 
   Size:       2126     856
 
PspOMI G'GGCC_C

Cuts at:      0     472    1990    2477    2982 
   Size:        472    1518     487     505

  Fragments arranged by size: 

                1518     505     487     472
 
PstI C_TGCA'G

Cuts at:      0    1554    2873    2982 
   Size:       1554    1319     109

  Fragments arranged by size: 

                1554    1319     109
 
PvuII CAG'CTG

Cuts at:      0    1164    1549    1673    2782    2874    2982 
   Size:       1164     385     124    1109      92     108

  Fragments arranged by size: 

                1164    1109     385     124     108      92
 
SacI G_AGCT'C

Cuts at:      0    1526    2982 
   Size:       1526    1456
 
SanDI GG'GwC_CC

Cuts at:      0     412    2982 
   Size:        412    2570
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2155    2982 
   Size:       2155     827
 
ScaI AGT'ACT

Cuts at:      0    1530    1984    2982 
   Size:       1530     454     998

  Fragments arranged by size: 

                1530     998     454
 
SexAI A'CCwGG_T

Cuts at:      0    2225    2982 
   Size:       2225     757
 
SfcI C'TryA_G

Cuts at:      0     773    1308    1550    2682    2869    2982 
   Size:        773     535     242    1132     187     113

  Fragments arranged by size: 

                1132     773     535     242     187     113
 
SfoI GGC'GCC

Cuts at:      0    1173    2982 
   Size:       1173    1809
 
SmaI CCC'GGG

Cuts at:      0    1301    1969    2058    2982 
   Size:       1301     668      89     924

  Fragments arranged by size: 

                1301     924     668      89
 
SmlI C'TyrA_G

Cuts at:      0     217    1339    2503    2982 
   Size:        217    1122    1164     479

  Fragments arranged by size: 

                1164    1122     479     217
 
StuI AGG'CCT

Cuts at:      0     905    1576    2982 
   Size:        905     671    1406

  Fragments arranged by size: 

                1406     905     671
 
StyI C'CwwG_G

Cuts at:      0     374     404     495    2021    2514    2735    2982 
   Size:        374      30      91    1526     493     221     247

  Fragments arranged by size: 

                1526     493     374     247     221      91      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     994    1136    1216    1653    2582    2691    2982 
   Size:        994     142      80     437     929     109     291

  Fragments arranged by size: 

                 994     929     437     291     142     109      80
 
TatI w'GTAC_w

Cuts at:      0    1528    1982    2982 
   Size:       1528     454    1000

  Fragments arranged by size: 

                1528    1000     454
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     263     295     297     329    2982 
   Size:        263      32       2      32    2653

  Fragments arranged by size: 

                2653     263      32      32       2
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     372     559    1150    1647    2215    2367    2982 
   Size:        372     187     591     497     568     152     615

  Fragments arranged by size: 

                 615     591     568     497     372     187     152
 
XmaI C'CCGG_G

Cuts at:      0    1299    1967    2056    2982 
   Size:       1299     668      89     926

  Fragments arranged by size: 

                1299     926     668      89
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AlfI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BglI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NspI     PasI     PciI    PflMI
     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SanDI
     SapI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI     AscI
     AseI    AsiSI     BbsI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmtI    BsaAI    BsiWI     BsmI    BspHI    BsrBI    BsrGI   BssHII
   BstEII     ClaI     DraI   DraIII     EciI    EcoRV     FalI     FseI
    FspAI  HindIII     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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