Restriction map for pF1KB6466
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BciVI       BfrI        BglI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspEI       BspHI       BspMI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        372  GGTACC               G^GTACC
AflIII        812  ACATGT               A^C[GA][CT]GT
AlwNI         826  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       372  GGTACC               G^GTACC
AvrII         876  CCTAGG               C^CTAGG
BanI          372  GGTACC               G^G[CT][GA]CC
BbsI          881  GAAGAC               GAAGAC(2/6)
BcgI          525  GCAACAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          887  TGATCA               T^GATCA
BglII         158  AGATCT               A^GATCT
BlnI          876  CCTAGG               C^CTAGG
BpmI          570  CTCCAG               CTGGAG(16/14)
BpuAI         881  GAAGAC               GAAGAC(2/6)
BseRI         988  GAGGAG               GAGGAG(10/8)
BsgI          925  CTGCAC               GTGCAG(16/14)
BsmI          194  GAATGC               GAATGC(1/-1)
BspDI         667  ATCGAT               AT^CGAT
BspLU11I      812  ACATGT               A^CATGT
BsrBI          97  GAGCGG               CCGCTC(-3/-3)
BstYI         158  AGATCT               [GA]^GATC[CT]
Cac8I         462  GCAAGC               GC[ACGT]^[ACGT]GC
ClaI          667  ATCGAT               AT^CGAT
EcoT14I       876  CCTAGG               C^C[AT][AT]GG
FbaI          887  TGATCA               T^GATCA
HaeII         319  AGCGCC               [GA]GCGC^[CT]
KpnI          372  GGTACC               GGTAC^C
MaeII         137  ACGT                 A^CGT
MflI          158  AGATCT               [GA]^GATC[CT]
NspI          812  ACATGT               [GA]CATG^[CT]
PstI          592  CTGCAG               CTGCA^G
SapI          576  GAAGAGC              GCTCTTC(1/4)
SfaNI         853  GATGC                GCATC(5/9)
SmlI          512  CTCAAG               C^T[CT][GA]AG
StyI          876  CCTAGG               C^C[AT][AT]GG
Tsp45I        716  GTGAC                ^GT[GC]AC
Tsp509I       623  AATT                 ^AATT
XhoII         158  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          352  GACGCC               G[GA]^CG[CT]C
              760  GACGCC              
ApaI           81  GGGCCC               GGGCC^C
              105  GGGCCC              
AspHI         567  GAGCTC               G[AT]GC[AT]^C
              991  GAGCTC              
BsaHI         352  GACGCC               G[GA]^CG[CT]C
              760  GACGCC              
BsiHKAI       567  GAGCTC               G[AT]GC[AT]^C
              991  GAGCTC              
BsmAI         865  GAGAC                GTCTC(1/5)
              971  GAGAC               
BstF5I        954  CATCC                GGATG(2/0)
              852  GGATG               
CfoI           32  GCGC                 GCG^C
              320  GCGC                
DdeI          222  CTCAG                C^T[ACGT]AG
              832  CTGAG               
FauI           29  CCCGC                CCCGC(4/6)
              119  CCCGC               
FokI          954  CATCC                GGATG(9/13)
              852  GGATG               
HgaI          352  GACGC                GACGC(5/10)
              760  GACGC               
HhaI           32  GCGC                 GCG^C
              320  GCGC                
Hin1I         352  GACGCC               G[GA]^CG[CT]C
              760  GACGCC              
HinP1I         32  GCGC                 G^CGC
              320  GCGC                
MaeIII        226  GTTAC                ^GT[ACGT]AC
              716  GTGAC               
SacI          567  GAGCTC               GAGCT^C
              991  GAGCTC              
SfcI          273  CTACAG               C^T[GA][CT]AG
              592  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BssKI       BstNI       BstUI       
Cfr13I      DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SmaI        TaqI        TfiI        
TseI        TthHB8I     XmaCI       XmaI        
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