Restriction map for pF1KB6466
 (Linear) (Six-base) MAPSORT of: KIBB6466.seq  Check: 6975  from: 1  to: 1056



KIBB6466.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

Acc65I G'GTAC_C

Cuts at:      0     372    1056 
   Size:        372     684
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     416     620    1056 
   Size:        193     223     204     436

  Fragments arranged by size: 

                 436     223     204     193
 
AflIII A'CryG_T

Cuts at:      0     812    1056 
   Size:        812     244
 
AlwNI CAG_nnn'CTG

Cuts at:      0     831    1056 
   Size:        831     225
 
ApaI G_GGCC'C

Cuts at:      0      85     109    1056 
   Size:         85      24     947

  Fragments arranged by size: 

                 947      85      24
 
AvaI C'yCGr_G

Cuts at:      0      78     108     710     968    1056 
   Size:         78      30     602     258      88

  Fragments arranged by size: 

                 602     258      88      78      30
 
AvrII C'CTAG_G

Cuts at:      0     876    1056 
   Size:        876     180
 
BanI G'GyrC_C

Cuts at:      0     372    1056 
   Size:        372     684
 
BanII G_rGCy'C

Cuts at:      0      85     109     178     571     995    1056 
   Size:         85      24      69     393     424      61

  Fragments arranged by size: 

                 424     393      85      69      61      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     888    1056 
   Size:        888     168
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     514     548    1056 
   Size:        514      34     508

  Fragments arranged by size: 

                 514     508      34
 
BclI T'GATC_A

Cuts at:      0     887    1056 
   Size:        887     169
 
BglII A'GATC_T

Cuts at:      0     158    1056 
   Size:        158     898
 
Bme1580I G_kGCm'C

Cuts at:      0      85     109    1056 
   Size:         85      24     947

  Fragments arranged by size: 

                 947      85      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555    1056 
   Size:        555     501
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497    1056 
   Size:        497     559
 
BsaHI Gr'CG_yC

Cuts at:      0     353     761    1056 
   Size:        353     408     295

  Fragments arranged by size: 

                 408     353     295
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796     982    1012    1056 
   Size:        766      30     186      30      44

  Fragments arranged by size: 

                 766     186      44      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1003    1056 
   Size:       1003      53
 
BseYI C'CCAG_C

Cuts at:      0     301     982    1056 
   Size:        301     681      74

  Fragments arranged by size: 

                 681     301      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     910    1056 
   Size:        910     146
 
BsiHKAI G_wGCw'C

Cuts at:      0     571     995    1056 
   Size:        571     424      61

  Fragments arranged by size: 

                 571     424      61
 
BsmI GAATG_Cn'

Cuts at:      0     200    1056 
   Size:        200     856
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     109     178     571     995    1056 
   Size:         85      24      69     393     424      61

  Fragments arranged by size: 

                 424     393      85      69      61      24
 
BsrBI CCG'CTC

Cuts at:      0      99    1056 
   Size:         99     957
 
BstYI r'GATC_y

Cuts at:      0     158    1056 
   Size:        158     898
 
BtgI C'CryG_G

Cuts at:      0     735     800    1042    1056 
   Size:        735      65     242      14

  Fragments arranged by size: 

                 735     242      65      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     438     945     960    1056 
   Size:        438     507      15      96

  Fragments arranged by size: 

                 507     438      96      15
 
ClaI AT'CG_AT

Cuts at:      0     668    1056 
   Size:        668     388
 
EarI CTCTTCn'nnn_

Cuts at:      0     209     281     571    1056 
   Size:        209      72     290     485

  Fragments arranged by size: 

                 485     290     209      72
 
EcoICRI GAG'CTC

Cuts at:      0     569     993    1056 
   Size:        569     424      63

  Fragments arranged by size: 

                 569     424      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     416     555     620    1056 
   Size:        193     223     139      65     436

  Fragments arranged by size: 

                 436     223     193     139      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     105     116     364     495     741     792    1056 
   Size:        105      11     248     131     246      51     264

  Fragments arranged by size: 

                 264     248     246     131     105      51      11
 
HaeII r_GCGC'y

Cuts at:      0     323    1056 
   Size:        323     733
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     580     612    1056 
   Size:        580      32     444

  Fragments arranged by size: 

                 580     444      32
 
KpnI G_GTAC'C

Cuts at:      0     376    1056 
   Size:        376     680
 
NspI r_CATG'y

Cuts at:      0     816    1056 
   Size:        816     240
 
PciI A'CATG_T

Cuts at:      0     812    1056 
   Size:        812     244
 
PfoI T'CCnGG_A

Cuts at:      0     571    1056 
   Size:        571     485
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204    1056 
   Size:        172      32     852

  Fragments arranged by size: 

                 852     172      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     364     741     792    1056 
   Size:        364     377      51     264

  Fragments arranged by size: 

                 377     364     264      51
 
PspOMI G'GGCC_C

Cuts at:      0      81     105    1056 
   Size:         81      24     951

  Fragments arranged by size: 

                 951      81      24
 
PstI C_TGCA'G

Cuts at:      0     596    1056 
   Size:        596     460
 
SacI G_AGCT'C

Cuts at:      0     571     995    1056 
   Size:        571     424      61

  Fragments arranged by size: 

                 571     424      61
 
SapI GCTCTTCn'nnn_

Cuts at:      0     571    1056 
   Size:        571     485
 
SfcI C'TryA_G

Cuts at:      0     273     592    1056 
   Size:        273     319     464

  Fragments arranged by size: 

                 464     319     273
 
SmaI CCC'GGG

Cuts at:      0      80     110     712    1056 
   Size:         80      30     602     344

  Fragments arranged by size: 

                 602     344      80      30
 
SmlI C'TyrA_G

Cuts at:      0     512    1056 
   Size:        512     544
 
StyI C'CwwG_G

Cuts at:      0     876    1056 
   Size:        876     180
 
TatI w'GTAC_w

Cuts at:      0     815    1056 
   Size:        815     241
 
XmaI C'CCGG_G

Cuts at:      0      78     108     710    1056 
   Size:         78      30     602     346

  Fragments arranged by size: 

                 602     346      78      30
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApaI     AvaI    AvrII     BanI
    BanII     BbsI     BcgI     BclI    BglII Bme1580I     BpmI    BpuEI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BsrBI    BstYI     BtgI    BtgZI     ClaI     EarI  EcoICRI  Eco57MI
 EcoO109I    HaeII    Hin4I     KpnI     NspI     PciI     PfoI     PpiI
    PpuMI   PspOMI     PstI     SacI     SapI     SfcI     SmaI     SmlI
     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtsI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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