Restriction map for pF1KB6425
 (Circular) (Six-base) MAPSORT of: pF1KB6425.seq  Check: 1817  from: 1  to: 4043



pF1KB6425  4043 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    244     244 
   Size:       4043
 
AccI GT'mk_AC

Cuts at:   1081    1081 
   Size:       4043
 
Acc65I G'GTAC_C

Cuts at:   1059    1059 
   Size:       4043
 
AclI AA'CG_TT

Cuts at:   3883    3883 
   Size:       4043
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    221    1868    2300    3047    3315     221 
   Size:       1647     432     747     268     949

  Fragments arranged by size: 

                1647     949     747     432     268
 
AflIII A'CryG_T

Cuts at:   1395    2499    3476    1395 
   Size:       1104     977    1962

  Fragments arranged by size: 

                1962    1104     977
 
AgeI A'CCGG_T

Cuts at:   2431    2431 
   Size:       4043
 
AlwNI CAG_nnn'CTG

Cuts at:    318    1348    2915     318 
   Size:       1030    1567    1446

  Fragments arranged by size: 

                1567    1446    1030
 
ApaI G_GGCC'C

Cuts at:    404     404 
   Size:       4043
 
ApaLI G'TGCA_C

Cuts at:   2813    2813 
   Size:       4043
 
ApoI r'AATT_y

Cuts at:   1027    1047    3396    3652    1027 
   Size:         20    2349     256    1418

  Fragments arranged by size: 

                2349    1418     256      20
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4043
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4043
 
AvaI C'yCGr_G

Cuts at:    455     753    1063     455 
   Size:        298     310    3435

  Fragments arranged by size: 

                3435     310     298
 
BamHI G'GATC_C

Cuts at:   1068    1068 
   Size:       4043
 
BanI G'GyrC_C

Cuts at:   1059    1473    1721    1756    1059 
   Size:        414     248      35    3346

  Fragments arranged by size: 

                3346     414     248      35
 
BanII G_rGCy'C

Cuts at:    404     551     724     899    1057    2087     404 
   Size:        147     173     175     158    1030    2360

  Fragments arranged by size: 

                2360    1030     175     173     158     147
 
BbeI G_GCGC'C

Cuts at:   1477    1725    1477 
   Size:        248    3795
 
BbvCI CC'TCA_GC

Cuts at:    683     683 
   Size:       4043
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    661     695     661 
   Size:         34    4009
 
BciVI GTATCCnnnnn_n'

Cuts at:   1941    2702    4043    1941 
   Size:        761    1341    1941

  Fragments arranged by size: 

                1941    1341     761
 
BclI T'GATC_A

Cuts at:   1563    1563 
   Size:       4043
 
BglI GCCn_nnn'nGGC

Cuts at:    538     829     538 
   Size:        291    3752
 
BglII A'GATC_T

Cuts at:   1558    3595    1558 
   Size:       2037    2006
 
BlpI GC'TnA_GC

Cuts at:    151    1155     151 
   Size:       1004    3039
 
Bme1580I G_kGCm'C

Cuts at:    404    1668    1761    2817     404 
   Size:       1264      93    1056    1630

  Fragments arranged by size: 

                1630    1264    1056      93
 
BmgBI CAC'GTC

Cuts at:    335     335 
   Size:       4043
 
BmrI ACTGGGnnnn_n'

Cuts at:    802    1255    1336    1671    3815     802 
   Size:        453      81     335    2144    1030

  Fragments arranged by size: 

                2144    1030     453     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2410    2410 
   Size:       4043
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    323     413     681     323 
   Size:         90     268    3685

  Fragments arranged by size: 

                3685     268      90
 
Bpu10I CC'TnA_GC

Cuts at:    246     683     246 
   Size:        437    3606
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1219    2098    2590    2888    1219 
   Size:        879     492     298    2374

  Fragments arranged by size: 

                2374     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    481     589    3747     481 
   Size:        108    3158     777

  Fragments arranged by size: 

                3158     777     108
 
BsaAI yAC'GTr

Cuts at:   1396    1396 
   Size:       4043
 
BsaBI GATnn'nnATC

Cuts at:      6    1557    3594       6 
   Size:       1551    2037     455

  Fragments arranged by size: 

                2037    1551     455
 
BsaHI Gr'CG_yC

Cuts at:   1474    1722    2424    3920    1474 
   Size:        248     702    1496    1597

  Fragments arranged by size: 

                1597    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1232    1753    2431    2705    2852    3263    1232 
   Size:        521     678     274     147     411    2012

  Fragments arranged by size: 

                2012     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    223     253     404     434     827     857     875     905     223 
   Size:         30     151      30     393      30      18      30    3361

  Fragments arranged by size: 

                3361     393     151      30      30      30      30      18
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    988     988 
   Size:       4043
 
BseYI C'CCAG_C

Cuts at:    616     708     864    1468    2226    2803    3299     616 
   Size:         92     156     604     758     577     496    1360

  Fragments arranged by size: 

                1360     758     604     577     496     156      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    272     272 
   Size:       4043
 
BsiEI CG_ry'CG

Cuts at:    104    1585    1631    2839     104 
   Size:       1481      46    1208    1308

  Fragments arranged by size: 

                1481    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1057    1835    2025    2817    1057 
   Size:        778     190     792    2283

  Fragments arranged by size: 

                2283     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    341     341 
   Size:       4043
 
Bsp1286I G_dGCh'C

Cuts at:    404     551     724     899    1057    1668    1761    1835    2025 
   Size:        147     173     175     158     611      93      74     190

Cuts at:   2025    2087    2817     404 
   Size:         62     730    1630

  Fragments arranged by size: 

                1630     730     611     190     175     173     158     147
                  93      74      62
 
BspEI T'CCGG_A

Cuts at:   3263    3263 
   Size:       4043
 
BspHI T'CATG_A

Cuts at:   3219    3219 
   Size:       4043
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    244    1093    1307    1609    1990     244 
   Size:        849     214     302     381    2297

  Fragments arranged by size: 

                2297     849     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2335    2389    3873    4038      50 
   Size:       2285      54    1484     165      55

  Fragments arranged by size: 

                2285    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    875    1955     875 
   Size:       1080    2963
 
BsrFI r'CCGG_y

Cuts at:    162     349     387    2041    2222    2431     162 
   Size:        187      38    1654     181     209    1774

  Fragments arranged by size: 

                1774    1654     209     187     181      38
 
BssHII G'CGCG_C

Cuts at:    114     140     114 
   Size:         26    4017
 
BssSI C'ACGA_G

Cuts at:    889    2314    2672     889 
   Size:       1425     358    2260

  Fragments arranged by size: 

                2260    1425     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1420    1420 
   Size:       4043
 
BstBI TT'CG_AA

Cuts at:   2404    2404 
   Size:       4043
 
BstXI CCAn_nnnn'nTGG

Cuts at:    354     354 
   Size:       4043
 
BstYI r'GATC_y

Cuts at:      1    1068    1535    1558    1893    2139    3151    3237    3249 
   Size:       1067     467      23     335     246    1012      86      12

Cuts at:   3249    3595       1 
   Size:        346     449

  Fragments arranged by size: 

                1067    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    536     779     824     536 
   Size:        243      45    3755

  Fragments arranged by size: 

                3755     243      45
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1993    2174    2337    1993 
   Size:        181     163    3699

  Fragments arranged by size: 

                3699     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1286    1286 
   Size:       4043
 
DraI TTT'AAA

Cuts at:   1042    1042 
   Size:       4043
 
DrdI GACnn_nn'nnGTC

Cuts at:   1749    2607    1749 
   Size:        858    3185
 
EaeI y'GGCC_r

Cuts at:    585     776    1628    1802    2193    2220    3565     585 
   Size:        191     852     174     391      27    1345    1063

  Fragments arranged by size: 

                1345    1063     852     391     191     174      27
 
EagI C'GGCC_G

Cuts at:   1628    1628 
   Size:       4043
 
EarI CTCTTCn'nnn_

Cuts at:   2066    2276    2066 
   Size:        210    3833
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2251    2561    2707    3853    2251 
   Size:        310     146    1146    2441

  Fragments arranged by size: 

                2441    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1055    1055 
   Size:       4043
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    221     323     413     681    1868    2300    3047    3315     221 
   Size:        102      90     268    1187     432     747     268     949

  Fragments arranged by size: 

                1187     949     747     432     268     268     102      90
            
 
EcoO109I rG'GnC_Cy

Cuts at:    400     613     658     830     840     998    1182     400 
   Size:        213      45     172      10     158     184    3261

  Fragments arranged by size: 

                3261     213     184     172     158      45      10
 
EcoRI G'AATT_C

Cuts at:   1027    1047    1027 
   Size:         20    4023
 
EcoRV GAT'ATC

Cuts at:    270     270 
   Size:       4043
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    646    2062     646 
   Size:       1416    2627
 
FspI TGC'GCA

Cuts at:   1824    1824 
   Size:       4043
 
HaeII r_GCGC'y

Cuts at:    124     151    1477    1725    2747    3726     124 
   Size:         27    1326     248    1022     979     441

  Fragments arranged by size: 

                1326    1022     979     441     248      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    223     255     495     527     679     711    1885    1915    1917 
   Size:         32     240      32     152      32    1174      30       2

Cuts at:   1917    1947     223 
   Size:         30    2319

  Fragments arranged by size: 

                2319    1174     240     152      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1082    1082 
   Size:       4043
 
KasI G'GCGC_C

Cuts at:   1473    1721    1473 
   Size:        248    3795
 
KpnI G_GTAC'C

Cuts at:   1063    1063 
   Size:       4043
 
MluI A'CGCG_T

Cuts at:   3476    3476 
   Size:       4043
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2714    2898    2714 
   Size:        184    3859
 
MscI TGG'CCA

Cuts at:    587    1804     587 
   Size:       1217    2826
 
MslI CAynn'nnrTG

Cuts at:   2032    2159    2032 
   Size:        127    3916
 
MspA1I CmG'CkG

Cuts at:    367    1154    1468    1828    2841    3086     367 
   Size:        787     314     360    1013     245    1324

  Fragments arranged by size: 

                1324    1013     787     360     314     245
 
NaeI GCC'GGC

Cuts at:    164     389    2224     164 
   Size:        225    1835    1983

  Fragments arranged by size: 

                1983    1835     225
 
NarI GG'CG_CC

Cuts at:   1474    1722    1474 
   Size:        248    3795
 
NgoMIV G'CCGG_C

Cuts at:    162     387    2222     162 
   Size:        225    1835    1983

  Fragments arranged by size: 

                1983    1835     225
 
NspI r_CATG'y

Cuts at:    579    1096    1596    2503     579 
   Size:        517     500     907    2119

  Fragments arranged by size: 

                2119     907     517     500
 
PasI CC'CwG_GG

Cuts at:    482     482 
   Size:       4043
 
PciI A'CATG_T

Cuts at:   2499    2499 
   Size:       4043
 
PfoI T'CCnGG_A

Cuts at:    845     845 
   Size:       4043
 
PmeI GTTT'AAAC

Cuts at:   1042    1042 
   Size:       4043
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3182    3214    3182 
   Size:         32    4011
 
PpuMI rG'GwC_Cy

Cuts at:    613     658     613 
   Size:         45    3998
 
PspOMI G'GGCC_C

Cuts at:    400     400 
   Size:       4043
 
PstI C_TGCA'G

Cuts at:    161     612    1090    1775     161 
   Size:        451     478     685    2429

  Fragments arranged by size: 

                2429     685     478     451
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4043
 
PvuII CAG'CTG

Cuts at:    367    1468    1828     367 
   Size:       1101     360    2582

  Fragments arranged by size: 

                2582    1101     360
 
RsrII CG'GwC_CG

Cuts at:   2238    2238 
   Size:       4043
 
SacI G_AGCT'C

Cuts at:   1057    1057 
   Size:       4043
 
SalI G'TCGA_C

Cuts at:   1080    1080 
   Size:       4043
 
SapI GCTCTTCn'nnn_

Cuts at:   2066    2276    2066 
   Size:        210    3833
 
SbfI CC_TGCA'GG

Cuts at:    612    1090     612 
   Size:        478    3565
 
ScaI AGT'ACT

Cuts at:   3560    3560 
   Size:       4043
 
SfcI C'TryA_G

Cuts at:     33     157     362     419     608     770    1086    1771    2764 
   Size:        124     205      57     189     162     316     685     993

Cuts at:   2764    2955      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     685     316     205     191     189     162
                 124      57
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    829     829 
   Size:       4043
 
SfoI GGC'GCC

Cuts at:   1475    1723    1475 
   Size:        248    3795
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4043
 
SmaI CCC'GGG

Cuts at:    457    1065     457 
   Size:        608    3435
 
SmlI C'TyrA_G

Cuts at:   1198    2113    2605    2867    1198 
   Size:        915     492     262    2374

  Fragments arranged by size: 

                2374     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4043
 
SphI G_CATG'C

Cuts at:   1096    1596    1096 
   Size:        500    3543
 
StuI AGG'CCT

Cuts at:    517     887     517 
   Size:        370    3673
 
StyI C'CwwG_G

Cuts at:    404     968    1177    1531     404 
   Size:        564     209     354    2916

  Fragments arranged by size: 

                2916     564     354     209
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1626    1759    2218    2427    1626 
   Size:        133     459     209    3242

  Fragments arranged by size: 

                3242     459     209     133
 
TatI w'GTAC_w

Cuts at:   3558    3558 
   Size:       4043
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    827     859     875     907    3560    3592     827 
   Size:         32      16      32    2653      32    1278

  Fragments arranged by size: 

                2653    1278      32      32      32      16
 
Tth111I GACn'n_nGTC

Cuts at:   1840    1840 
   Size:       4043
 
XbaI T'CTAG_A

Cuts at:   1074    1074 
   Size:       4043
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    649    1538     649 
   Size:        889    3154
 
XmaI C'CCGG_G

Cuts at:    455    1063     455 
   Size:        608    3435
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BfrBI     BmtI    BsiWI     BsmI    BsrGI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     XmnI     ZraI
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