Restriction map for pF1KB6854
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmFI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MaeI        MamI        MfeI        MluI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfoI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII        110  TCCGGA               T^CCGGA
AcsI          139  AAATTT               [GA]^AATT[CT]
ApoI          139  AAATTT               [GA]^AATT[CT]
BanII         106  GGGCTC               G[GA]GC[CT]^C
BbsI          340  GTCTTC               GAAGAC(2/6)
BbvI          231  GCTGC                GCAGC(8/12)
BglI          503  GCCGCCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          106  GGGCTC               G[AGT]GC[ACT]^C
BpuAI         340  GTCTTC               GAAGAC(2/6)
BsaI           51  GAGACC               GGTCTC(1/5)
BsaWI         110  TCCGGA               [AT]^CCGG[AT]
BseAI         110  TCCGGA               T^CCGGA
BsmI          528  GAATGC               GAATGC(1/-1)
Bsp1286I      106  GGGCTC               G[AGT]GC[ACT]^C
BspEI         110  TCCGGA               T^CCGGA
BspMI         417  GCAGGT               ACCTGC(4/8)
BsrI            4  CCAGT                ACTGG(1/-1)
BstBI         214  TTCGAA               TT^CGAA
BstYI         155  AGATCC               [GA]^GATC[CT]
DdeI          315  CTTAG                C^T[ACGT]AG
DraII         197  AGGACCC              [GA]G^G[ACGT]CC[CT]
EarI          519  GAAGAG               CTCTTC(1/4)
EcoO109I      197  AGGACCC              [GA]G^G[ACGT]CC[CT]
FauI           45  CCCGC                CCCGC(4/6)
HaeII         395  AGCGCC               [GA]GCGC^[CT]
HinfI         454  GATTC                G^A[ACGT]TC
HphI          423  GGTGA                GGTGA(8/7)
Ksp632I       519  GAAGAG               CTCTTC(1/4)
KspI           46  CCGCGG               CCGC^GG
MaeII         120  ACGT                 A^CGT
MaeIII         40  GTGAC                ^GT[ACGT]AC
MflI          155  AGATCC               [GA]^GATC[CT]
MroI          110  TCCGGA               T^CCGGA
MslI          249  CATTGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         46  CCGCGG               C[AC]G^C[GT]G
NspV          214  TTCGAA               TT^CGAA
PpuMI         197  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         333  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          328  CTGCAG               CTGCA^G
SacII          46  CCGCGG               CCGC^GG
SfcI          328  CTGCAG               C^T[GA][CT]AG
SfiI          502  GGCCGCCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          214  TTCGAA               TT^CGAA
TfiI          454  GATTC                G^A[AT]TC
TseI          231  GCTGC                G^C[AT]GC
Tsp45I         40  GTGAC                ^GT[GC]AC
Tsp509I       140  AATT                 ^AATT
TspRI           5  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          543  CCAGGGTGGGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         155  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          206  GGCGTC               G[GA]^CG[CT]C
              323  GACGCC              
AlwI          156  GATCC                GGATC(4/5)
              387  GATCC               
AvaI          170  CCCGGG               C^[CT]CG[GA]G
              507  CCCGGG              
BalI          149  TGGCCA               TGG^CCA
              356  TGGCCA              
BpmI          129  CTGGAG               CTGGAG(16/14)
              560  CTGGAG              
BsaHI         206  GGCGTC               G[GA]^CG[CT]C
              323  GACGCC              
BseRI         571  GAGGAG               GAGGAG(10/8)
              574  GAGGAG              
BsoBI         170  CCCGGG               C^[CT]CG[GA]G
              507  CCCGGG              
CfoI          396  GCGC                 GCG^C
              483  GCGC                
DsaI           46  CCGCGG               C^C[GA][CT]GG
              202  CCACGG              
HhaI          396  GCGC                 GCG^C
              483  GCGC                
Hin1I         206  GGCGTC               G[GA]^CG[CT]C
              323  GACGCC              
HinP1I        396  GCGC                 G^CGC
              483  GCGC                
MluNI         149  TGGCCA               TGG^CCA
              356  TGGCCA              
MscI          149  TGGCCA               TGG^CCA
              356  TGGCCA              
SmaI          170  CCCGGG               CCC^GGG
              507  CCCGGG              
XmaCI         170  CCCGGG               C^CCGGG
              507  CCCGGG              
XmaI          170  CCCGGG               C^CCGGG
              507  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BcnI        
BsaJI       BsiYI       BslI        BsmAI       BsmBI       
BssKI       BstF5I      BstNI       BstUI       Cac8I       
Cfr13I      DpnII       EaeI        EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HgaI        HpaII       
ItaI        MboI        MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       Sau3AI      Sau96I      ScrFI       TaqI        
TthHB8I     
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