Restriction map for pF1KB6856
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeIII      Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MflI        MluI        MluNI       MroI        
MscI        MslI        NarI        NciI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        Tsp45I      Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AciI          432  CCGC                 CCGC(-3/-1)
Alw44I        373  GTGCAC               G^TGCAC
ApaLI         373  GTGCAC               G^TGCAC
AspHI         373  GTGCAC               G[AT]GC[AT]^C
AspI          394  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         102  GGACC                G^G[AT]CC
BbsI          400  GTCTTC               GAAGAC(2/6)
BlpI          112  GCTCAGC              GC^T[ACGT]AGC
BmyI          373  GTGCAC               G[AGT]GC[ACT]^C
Bpu1102I      112  GCTCAGC              GC^T[ACGT]AGC
BpuAI         400  GTCTTC               GAAGAC(2/6)
BseRI         291  GAGGAG               GAGGAG(10/8)
BsiHKAI       373  GTGCAC               G[AT]GC[AT]^C
BsmFI         347  GTCCC                GGGAC(10/14)
Bsp1286I      373  GTGCAC               G[AGT]GC[ACT]^C
BsrFI         452  GCCGGC               [GA]^CCGG[CT]
Cac8I         452  GCCGGC               GC[ACGT]^[ACGT]GC
CelII         112  GCTCAGC              GC^T[ACGT]AGC
CfoI           14  GCGC                 GCG^C
Cfr10I        452  GCCGGC               [GA]^CCGG[CT]
Cfr13I        102  GGACC                G^G[ACGT]CC
DpnII          21  GATC                 ^GATC
EarI           77  CTCTTC               CTCTTC(1/4)
HaeII          13  AGCGCC               [GA]GCGC^[CT]
HhaI           14  GCGC                 GCG^C
HinP1I         14  GCGC                 G^CGC
Ksp632I        77  CTCTTC               CTCTTC(1/4)
MboI           21  GATC                 ^GATC
MfeI          577  CAATTG               C^AATTG
MseI          554  TTAA                 T^TAA
MspA1I        368  CAGCTG               C[AC]G^C[GT]G
MunI          577  CAATTG               C^AATTG
MwoI          117  GCTCAGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI          452  GCCGGC               GCC^GGC
NdeII          21  GATC                 ^GATC
NgoMIV        452  GCCGGC               G^CCGGC
NlaIII        271  CATG                 CATG^
NlaIV         353  GGAGCC               GG[ACGT]^[ACGT]CC
NspI          270  GCATGT               [GA]CATG^[CT]
PflFI         394  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           58  GAGTC                GAGTC(4/5)
PvuII         368  CAGCTG               CAG^CTG
SapI           76  GCTCTTC              GCTCTTC(1/4)
Sau3AI         21  GATC                 ^GATC
Sau96I        102  GGACC                G^G[ACGT]CC
SspI          130  AATATT               AAT^ATT
TfiI          428  GAATC                G^A[AT]TC
Tru9I         554  TTAA                 T^TAA
TspRI         261  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       394  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         241  CGCG                 CG^CG
              433  CGCG                
AcsI          296  GAATTC               [GA]^AATT[CT]
              562  GAATTC              
ApoI          296  GAATTC               [GA]^AATT[CT]
              562  GAATTC              
Asp700I       207  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              252  GAAAAAGTTC          
BbvI          139  GCAGC                GCAGC(8/12)
              449  GCAGC               
BfaI          235  CTAG                 C^TAG
              442  CTAG                
BpmI           82  CTGGAG               CTGGAG(16/14)
               88  CTGGAG              
BsaJI          71  CCCAGG               C^C[ACGT][ACGT]GG
              349  CCCAGG              
BssKI          72  CCAGG                ^CC[ACGT]GG
              350  CCAGG               
BstF5I        384  CATCC                GGATG(2/0)
              549  GGATG               
BstNI          72  CCAGG                CC^[AT]GG
              350  CCAGG               
BstUI         241  CGCG                 CG^CG
              433  CGCG                
EcoRI         296  GAATTC               G^AATTC
              562  GAATTC              
EcoRII         72  CCAGG                ^CC[AT]GG
              350  CCAGG               
Fnu4HI        139  GCAGC                GC^[ACGT]GC
              449  GCAGC               
FokI          384  CATCC                GGATG(9/13)
              549  GGATG               
HgaI          345  GCGTC                GACGC(5/10)
              434  GCGTC               
HinfI          58  GAGTC                G^A[ACGT]TC
              428  GAATC               
ItaI          139  GCAGC                GC^[ACGT]GC
              449  GCAGC               
MaeI          235  CTAG                 C^TAG
              442  CTAG                
MboII          78  TCTTC                GAAGA(8/7)
              401  TCTTC               
MvaI           72  CCAGG                CC^[AT]GG
              350  CCAGG               
MvnI          241  CGCG                 CG^CG
              433  CGCG                
ScrFI          72  CCAGG                CC^[ACGT]GG
              350  CCAGG               
SfaNI         379  GATGC                GCATC(5/9)
               65  GCATC               
SmlI          106  CTCAAG               C^T[CT][GA]AG
              388  CTTGAG              
TseI          139  GCAGC                G^C[AT]GC
              449  GCAGC               
XmnI          207  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              252  GAAAAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AfaI        AluI        DdeI        HapII       HpaII       
MnlI        MspI        RsaI        TaqI        Tsp509I     
TthHB8I     
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