Restriction map for pF1KB6856
 (Circular) (Six-base) MAPSORT of: pF1KB6856.seq  Check: 4606  from: 1  to: 3695



pF1KB6856  3695 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    733     733 
   Size:       3695
 
Acc65I G'GTAC_C

Cuts at:    711     711 
   Size:       3695
 
AclI AA'CG_TT

Cuts at:   3535    3535 
   Size:       3695
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1520    1952    2699    2967    1520 
   Size:        432     747     268    2248

  Fragments arranged by size: 

                2248     747     432     268
 
AflIII A'CryG_T

Cuts at:   1047    2151    3128    1047 
   Size:       1104     977    1614

  Fragments arranged by size: 

                1614    1104     977
 
AgeI A'CCGG_T

Cuts at:   2083    2083 
   Size:       3695
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    349     381     349 
   Size:         32    3663
 
AlwNI CAG_nnn'CTG

Cuts at:   1000    2567    1000 
   Size:       1567    2128
 
ApaLI G'TGCA_C

Cuts at:    481    2465     481 
   Size:       1984    1711
 
ApoI r'AATT_y

Cuts at:    404     670     699    3048    3304     404 
   Size:        266      29    2349     256     795

  Fragments arranged by size: 

                2349     795     266     256      29
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3695
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3695
 
AvaI C'yCGr_G

Cuts at:    715     715 
   Size:       3695
 
BamHI G'GATC_C

Cuts at:    720     720 
   Size:       3695
 
BanI G'GyrC_C

Cuts at:    711    1125    1373    1408     711 
   Size:        414     248      35    2998

  Fragments arranged by size: 

                2998     414     248      35
 
BanII G_rGCy'C

Cuts at:    709    1739     709 
   Size:       1030    2665
 
BbeI G_GCGC'C

Cuts at:   1129    1377    1129 
   Size:        248    3447
 
BbsI GAAGACnn'nnnn_

Cuts at:    501     501 
   Size:       3695
 
BciVI GTATCCnnnnn_n'

Cuts at:   1593    2354    3695    1593 
   Size:        761    1341    1593

  Fragments arranged by size: 

                1593    1341     761
 
BclI T'GATC_A

Cuts at:   1215    1215 
   Size:       3695
 
BglII A'GATC_T

Cuts at:   1210    3247    1210 
   Size:       2037    1658
 
BlpI GC'TnA_GC

Cuts at:    221     807     221 
   Size:        586    3109
 
Bme1580I G_kGCm'C

Cuts at:    485    1320    1413    2469     485 
   Size:        835      93    1056    1711

  Fragments arranged by size: 

                1711    1056     835      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    907     988    1323    3467     907 
   Size:         81     335    2144    1135

  Fragments arranged by size: 

                2144    1135     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    222     485    2062     222 
   Size:        263    1577    1855

  Fragments arranged by size: 

                1855    1577     263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    211     217     211 
   Size:          6    3689
 
Bpu10I CC'TnA_GC

Cuts at:    226     251     413     226 
   Size:         25     162    3508

  Fragments arranged by size: 

                3508     162      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    199     517     871    1750    2242    2540     199 
   Size:        318     354     879     492     298    1354

  Fragments arranged by size: 

                1354     879     492     354     318     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3399    3399 
   Size:       3695
 
BsaAI yAC'GTr

Cuts at:   1048    1048 
   Size:       3695
 
BsaBI GATnn'nnATC

Cuts at:      6    1209    3246       6 
   Size:       1203    2037     455

  Fragments arranged by size: 

                2037    1203     455
 
BsaHI Gr'CG_yC

Cuts at:   1126    1374    2076    3572    1126 
   Size:        248     702    1496    1249

  Fragments arranged by size: 

                1496    1249     702     248
 
BsaWI w'CCGG_w

Cuts at:    884    1405    2083    2357    2504    2915     884 
   Size:        521     678     274     147     411    1664

  Fragments arranged by size: 

                1664     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    349     379     349 
   Size:         30    3665
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    414     414 
   Size:       3695
 
BseYI C'CCAG_C

Cuts at:   1120    1878    2455    2951    1120 
   Size:        758     577     496    1864

  Fragments arranged by size: 

                1864     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1237    1283    2491     104 
   Size:       1133      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1133      46
 
BsiHKAI G_wGCw'C

Cuts at:    485     709    1487    1677    2469     485 
   Size:        224     778     190     792    1711

  Fragments arranged by size: 

                1711     792     778     224     190
 
Bsp1286I G_dGCh'C

Cuts at:    485     709    1320    1413    1487    1677    1739    2469     485 
   Size:        224     611      93      74     190      62     730    1711

  Fragments arranged by size: 

                1711     730     611     224     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2915    2915 
   Size:       3695
 
BspHI T'CATG_A

Cuts at:   2871    2871 
   Size:       3695
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    745     959    1261    1642     745 
   Size:        214     302     381    2798

  Fragments arranged by size: 

                2798     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1987    2041    3525    3690      50 
   Size:       1937      54    1484     165      55

  Fragments arranged by size: 

                1937    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1607    1607 
   Size:       3695
 
BsrFI r'CCGG_y

Cuts at:    560    1693    1874    2083     560 
   Size:       1133     181     209    2172

  Fragments arranged by size: 

                2172    1133     209     181
 
BssSI C'ACGA_G

Cuts at:   1966    2324    1966 
   Size:        358    3337
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1072    1072 
   Size:       3695
 
BstBI TT'CG_AA

Cuts at:   2056    2056 
   Size:       3695
 
BstYI r'GATC_y

Cuts at:      1     720    1187    1210    1545    1791    2803    2889    2901 
   Size:        719     467      23     335     246    1012      86      12

Cuts at:   2901    3247       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     719     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1645    1826    1989    1645 
   Size:        181     163    3351

  Fragments arranged by size: 

                3351     181     163
 
BtsI GCAGTG_nn'

Cuts at:    938     938 
   Size:       3695
 
DraI TTT'AAA

Cuts at:    694     694 
   Size:       3695
 
DrdI GACnn_nn'nnGTC

Cuts at:   1401    2259    1401 
   Size:        858    2837
 
EaeI y'GGCC_r

Cuts at:   1280    1454    1845    1872    3217    1280 
   Size:        174     391      27    1345    1758

  Fragments arranged by size: 

                1758    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1280    1280 
   Size:       3695
 
EarI CTCTTCn'nnn_

Cuts at:    191    1718    1928     191 
   Size:       1527     210    1958

  Fragments arranged by size: 

                1958    1527     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1903    2213    2359    3505    1903 
   Size:        310     146    1146    2093

  Fragments arranged by size: 

                2093    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    707     707 
   Size:       3695
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    211     217    1520    1952    2699    2967     211 
   Size:          6    1303     432     747     268     939

  Fragments arranged by size: 

                1303     939     747     432     268       6
 
EcoO109I rG'GnC_Cy

Cuts at:    834     834 
   Size:       3695
 
EcoRI G'AATT_C

Cuts at:    404     670     699     404 
   Size:        266      29    3400

  Fragments arranged by size: 

                3400     266      29
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1714    1714 
   Size:       3695
 
FspI TGC'GCA

Cuts at:   1476    1476 
   Size:       3695
 
HaeII r_GCGC'y

Cuts at:    125    1129    1377    2399    3378     125 
   Size:       1004     248    1022     979     442

  Fragments arranged by size: 

                1022    1004     979     442     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    151     183    1537    1567    1569    1599     151 
   Size:         32    1354      30       2      30    2247

  Fragments arranged by size: 

                2247    1354      32      30      30       2
 
HincII GTy'rAC

Cuts at:    734     734 
   Size:       3695
 
KasI G'GCGC_C

Cuts at:   1125    1373    1125 
   Size:        248    3447
 
KpnI G_GTAC'C

Cuts at:    715     715 
   Size:       3695
 
MfeI C'AATT_G

Cuts at:    685     685 
   Size:       3695
 
MluI A'CGCG_T

Cuts at:   3128    3128 
   Size:       3695
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    256    2366    2550     256 
   Size:       2110     184    1401

  Fragments arranged by size: 

                2110    1401     184
 
MscI TGG'CCA

Cuts at:   1456    1456 
   Size:       3695
 
MslI CAynn'nnrTG

Cuts at:   1684    1811    1684 
   Size:        127    3568
 
MspA1I CmG'CkG

Cuts at:    478     806    1120    1480    2493    2738     478 
   Size:        328     314     360    1013     245    1435

  Fragments arranged by size: 

                1435    1013     360     328     314     245
 
NaeI GCC'GGC

Cuts at:    562    1876     562 
   Size:       1314    2381
 
NarI GG'CG_CC

Cuts at:   1126    1374    1126 
   Size:        248    3447
 
NgoMIV G'CCGG_C

Cuts at:    560    1874     560 
   Size:       1314    2381
 
NspI r_CATG'y

Cuts at:    382     748    1248    2155     382 
   Size:        366     500     907    1922

  Fragments arranged by size: 

                1922     907     500     366
 
PciI A'CATG_T

Cuts at:   2151    2151 
   Size:       3695
 
PmeI GTTT'AAAC

Cuts at:    694     694 
   Size:       3695
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    349     381    2834    2866     349 
   Size:         32    2453      32    1178

  Fragments arranged by size: 

                2453    1178      32      32
 
PstI C_TGCA'G

Cuts at:    742    1427     742 
   Size:        685    3010
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3695
 
PvuII CAG'CTG

Cuts at:    478    1120    1480     478 
   Size:        642     360    2693

  Fragments arranged by size: 

                2693     642     360
 
RsrII CG'GwC_CG

Cuts at:   1890    1890 
   Size:       3695
 
SacI G_AGCT'C

Cuts at:    709     709 
   Size:       3695
 
SalI G'TCGA_C

Cuts at:    732     732 
   Size:       3695
 
SapI GCTCTTCn'nnn_

Cuts at:    191    1718    1928     191 
   Size:       1527     210    1958

  Fragments arranged by size: 

                1958    1527     210
 
SbfI CC_TGCA'GG

Cuts at:    742     742 
   Size:       3695
 
ScaI AGT'ACT

Cuts at:   3212    3212 
   Size:       3695
 
SfcI C'TryA_G

Cuts at:     33     738    1423    2416    2607      33 
   Size:        705     685     993     191    1121

  Fragments arranged by size: 

                1121     993     705     685     191
 
SfoI GGC'GCC

Cuts at:   1127    1375    1127 
   Size:        248    3447
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3695
 
SmaI CCC'GGG

Cuts at:    717     717 
   Size:       3695
 
SmlI C'TyrA_G

Cuts at:    214     496     850    1765    2257    2519     214 
   Size:        282     354     915     492     262    1390

  Fragments arranged by size: 

                1390     915     492     354     282     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3695
 
SphI G_CATG'C

Cuts at:    748    1248     748 
   Size:        500    3195
 
SspI AAT'ATT

Cuts at:    240     240 
   Size:       3695
 
StyI C'CwwG_G

Cuts at:    829    1183     829 
   Size:        354    3341
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1278    1411    1870    2079    1278 
   Size:        133     459     209    2894

  Fragments arranged by size: 

                2894     459     209     133
 
TatI w'GTAC_w

Cuts at:   3210    3210 
   Size:       3695
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3212    3244    3212 
   Size:         32    3663
 
Tth111I GACn'n_nGTC

Cuts at:    505    1492     505 
   Size:        987    2708
 
XbaI T'CTAG_A

Cuts at:    726     726 
   Size:       3695
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1190    1190 
   Size:       3695
 
XmaI C'CCGG_G

Cuts at:    715     715 
   Size:       3695
 
XmnI GAAnn'nnTTC

Cuts at:    319     364     319 
   Size:         45    3650
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PmeI
     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI     XhoI
     ZraI
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