Restriction map for pF1KB6807
 (Circular) (Six-base) MAPSORT of: pF1KB6807.seq  Check: 8683  from: 1  to: 3590



pF1KB6807  3590 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    628     628 
   Size:       3590
 
Acc65I G'GTAC_C

Cuts at:    606     606 
   Size:       3590
 
AclI AA'CG_TT

Cuts at:   3430    3430 
   Size:       3590
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    515     563    1415    1847    2594    2862     515 
   Size:         48     852     432     747     268    1243

  Fragments arranged by size: 

                1243     852     747     432     268      48
 
AflII C'TTAA_G

Cuts at:    202     202 
   Size:       3590
 
AflIII A'CryG_T

Cuts at:    942    2046    3023     942 
   Size:       1104     977    1509

  Fragments arranged by size: 

                1509    1104     977
 
AgeI A'CCGG_T

Cuts at:   1978    1978 
   Size:       3590
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    205     237     205 
   Size:         32    3558
 
AlwNI CAG_nnn'CTG

Cuts at:    895    2462     895 
   Size:       1567    2023
 
ApaLI G'TGCA_C

Cuts at:    305    2360     305 
   Size:       2055    1535
 
ApoI r'AATT_y

Cuts at:    594    2943    3199     594 
   Size:       2349     256     985

  Fragments arranged by size: 

                2349     985     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3590
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3590
 
AvaI C'yCGr_G

Cuts at:    610     610 
   Size:       3590
 
BamHI G'GATC_C

Cuts at:    615     615 
   Size:       3590
 
BanI G'GyrC_C

Cuts at:    606    1020    1268    1303     606 
   Size:        414     248      35    2893

  Fragments arranged by size: 

                2893     414     248      35
 
BanII G_rGCy'C

Cuts at:    455     604    1634     455 
   Size:        149    1030    2411

  Fragments arranged by size: 

                2411    1030     149
 
BbeI G_GCGC'C

Cuts at:   1024    1272    1024 
   Size:        248    3342
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    376     410     376 
   Size:         34    3556
 
BciVI GTATCCnnnnn_n'

Cuts at:   1488    2249    3590    1488 
   Size:        761    1341    1488

  Fragments arranged by size: 

                1488    1341     761
 
BclI T'GATC_A

Cuts at:   1110    1110 
   Size:       3590
 
BglII A'GATC_T

Cuts at:   1105    3142    1105 
   Size:       2037    1553
 
BlpI GC'TnA_GC

Cuts at:    702     702 
   Size:       3590
 
Bme1580I G_kGCm'C

Cuts at:    309     438    1215    1308    2364     309 
   Size:        129     777      93    1056    1535

  Fragments arranged by size: 

                1535    1056     777     129      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    802     883    1218    3362     802 
   Size:         81     335    2144    1030

  Fragments arranged by size: 

                2144    1030     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1957    1957 
   Size:       3590
 
Bpu10I CC'TnA_GC

Cuts at:    454     542     454 
   Size:         88    3502
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    766    1645    2137    2435     766 
   Size:        879     492     298    1921

  Fragments arranged by size: 

                1921     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3294    3294 
   Size:       3590
 
BsaAI yAC'GTr

Cuts at:    310     943     310 
   Size:        633    2957
 
BsaBI GATnn'nnATC

Cuts at:      6     195    1104    3141       6 
   Size:        189     909    2037     455

  Fragments arranged by size: 

                2037     909     455     189
 
BsaHI Gr'CG_yC

Cuts at:   1021    1269    1971    3467    1021 
   Size:        248     702    1496    1144

  Fragments arranged by size: 

                1496    1144     702     248
 
BsaWI w'CCGG_w

Cuts at:    779    1300    1978    2252    2399    2810     779 
   Size:        521     678     274     147     411    1559

  Fragments arranged by size: 

                1559     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    525    1015    1773    2350    2846     525 
   Size:        490     758     577     496    1269

  Fragments arranged by size: 

                1269     758     577     496     490
 
BsiEI CG_ry'CG

Cuts at:    104     390    1132    1178    2386     104 
   Size:        286     742      46    1208    1308

  Fragments arranged by size: 

                1308    1208     742     286      46
 
BsiHKAI G_wGCw'C

Cuts at:    309     432     604    1382    1572    2364     309 
   Size:        123     172     778     190     792    1535

  Fragments arranged by size: 

                1535     792     778     190     172     123
 
Bsp1286I G_dGCh'C

Cuts at:    309     432     438     455     604    1215    1308    1382    1572 
   Size:        123       6      17     149     611      93      74     190

Cuts at:   1572    1634    2364     309 
   Size:         62     730    1535

  Fragments arranged by size: 

                1535     730     611     190     149     123      93      74
                  62      17       6
 
BspEI T'CCGG_A

Cuts at:   2810    2810 
   Size:       3590
 
BspHI T'CATG_A

Cuts at:   2766    2766 
   Size:       3590
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    640     854    1156    1537     640 
   Size:        214     302     381    2693

  Fragments arranged by size: 

                2693     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1882    1936    3420    3585      50 
   Size:       1832      54    1484     165      55

  Fragments arranged by size: 

                1832    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    401    1502     401 
   Size:       1101    2489
 
BsrFI r'CCGG_y

Cuts at:    464    1588    1769    1978     464 
   Size:       1124     181     209    2076

  Fragments arranged by size: 

                2076    1124     209     181
 
BssSI C'ACGA_G

Cuts at:    431    1861    2219     431 
   Size:       1430     358    1802

  Fragments arranged by size: 

                1802    1430     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    967     967 
   Size:       3590
 
BstBI TT'CG_AA

Cuts at:   1951    1951 
   Size:       3590
 
BstYI r'GATC_y

Cuts at:      1     359     615    1082    1105    1440    1686    2698    2784 
   Size:        358     256     467      23     335     246    1012      86

Cuts at:   2784    2796    3142       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     358     346     335     256     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    226     226 
   Size:       3590
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3590
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1540    1721    1884    1540 
   Size:        181     163    3246

  Fragments arranged by size: 

                3246     181     163
 
BtsI GCAGTG_nn'

Cuts at:    833     833 
   Size:       3590
 
DraI TTT'AAA

Cuts at:    589     589 
   Size:       3590
 
DrdI GACnn_nn'nnGTC

Cuts at:   1296    2154    1296 
   Size:        858    2732
 
EaeI y'GGCC_r

Cuts at:    110     466    1175    1349    1740    1767    3112     110 
   Size:        356     709     174     391      27    1345     588

  Fragments arranged by size: 

                1345     709     588     391     356     174      27
 
EagI C'GGCC_G

Cuts at:   1175    1175 
   Size:       3590
 
EarI CTCTTCn'nnn_

Cuts at:   1613    1823    1613 
   Size:        210    3380
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1798    2108    2254    3400    1798 
   Size:        310     146    1146    1988

  Fragments arranged by size: 

                1988    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    602     602 
   Size:       3590
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    515     563    1415    1847    2594    2862     515 
   Size:         48     852     432     747     268    1243

  Fragments arranged by size: 

                1243     852     747     432     268      48
 
EcoO109I rG'GnC_Cy

Cuts at:    264     327     564     729     264 
   Size:         63     237     165    3125

  Fragments arranged by size: 

                3125     237     165      63
 
EcoRI G'AATT_C

Cuts at:    594     594 
   Size:       3590
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1609    1609 
   Size:       3590
 
FspI TGC'GCA

Cuts at:   1371    1371 
   Size:       3590
 
HaeII r_GCGC'y

Cuts at:   1024    1272    2294    3273    1024 
   Size:        248    1022     979    1341

  Fragments arranged by size: 

                1341    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1432    1462    1464    1494    1432 
   Size:         30       2      30    3528

  Fragments arranged by size: 

                3528      30      30       2
 
HincII GTy'rAC

Cuts at:    629     629 
   Size:       3590
 
HindIII A'AGCT_T

Cuts at:    415     415 
   Size:       3590
 
KasI G'GCGC_C

Cuts at:   1020    1268    1020 
   Size:        248    3342
 
KpnI G_GTAC'C

Cuts at:    610     610 
   Size:       3590
 
MluI A'CGCG_T

Cuts at:   3023    3023 
   Size:       3590
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2261    2445    2261 
   Size:        184    3406
 
MscI TGG'CCA

Cuts at:    112    1351     112 
   Size:       1239    2351
 
MslI CAynn'nnrTG

Cuts at:    161    1579    1706     161 
   Size:       1418     127    2045

  Fragments arranged by size: 

                2045    1418     127
 
MspA1I CmG'CkG

Cuts at:    701    1015    1375    2388    2633     701 
   Size:        314     360    1013     245    1658

  Fragments arranged by size: 

                1658    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    466    1771     466 
   Size:       1305    2285
 
NarI GG'CG_CC

Cuts at:   1021    1269    1021 
   Size:        248    3342
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3590
 
NgoMIV G'CCGG_C

Cuts at:    464    1769     464 
   Size:       1305    2285
 
NspI r_CATG'y

Cuts at:    237     643    1143    2050     237 
   Size:        406     500     907    1777

  Fragments arranged by size: 

                1777     907     500     406
 
PciI A'CATG_T

Cuts at:   2046    2046 
   Size:       3590
 
PmeI GTTT'AAAC

Cuts at:    589     589 
   Size:       3590
 
PmlI CAC'GTG

Cuts at:    310     310 
   Size:       3590
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2729    2761    2729 
   Size:         32    3558
 
PstI C_TGCA'G

Cuts at:    522     637    1322     522 
   Size:        115     685    2790

  Fragments arranged by size: 

                2790     685     115
 
PvuI CG_AT'CG

Cuts at:    104     390     104 
   Size:        286    3304
 
PvuII CAG'CTG

Cuts at:   1015    1375    1015 
   Size:        360    3230
 
RsrII CG'GwC_CG

Cuts at:   1785    1785 
   Size:       3590
 
SacI G_AGCT'C

Cuts at:    604     604 
   Size:       3590
 
SalI G'TCGA_C

Cuts at:    627     627 
   Size:       3590
 
SapI GCTCTTCn'nnn_

Cuts at:   1613    1823    1613 
   Size:        210    3380
 
SbfI CC_TGCA'GG

Cuts at:    637     637 
   Size:       3590
 
ScaI AGT'ACT

Cuts at:   3107    3107 
   Size:       3590
 
SfcI C'TryA_G

Cuts at:     33     518     633    1318    2311    2502      33 
   Size:        485     115     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     485     191     115
 
SfoI GGC'GCC

Cuts at:   1022    1270    1022 
   Size:        248    3342
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3590
 
SmaI CCC'GGG

Cuts at:    612     612 
   Size:       3590
 
SmlI C'TyrA_G

Cuts at:    202     745    1660    2152    2414     202 
   Size:        543     915     492     262    1378

  Fragments arranged by size: 

                1378     915     543     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3590
 
SphI G_CATG'C

Cuts at:    643    1143     643 
   Size:        500    3090
 
StyI C'CwwG_G

Cuts at:    107     293     341     446     476     482     488     494     506 
   Size:        186      48     105      30       6       6       6      12

Cuts at:    506     724    1078     107 
   Size:        218     354    2619

  Fragments arranged by size: 

                2619     354     218     186     105      48      30      12
                   6       6       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1173    1306    1765    1974    1173 
   Size:        133     459     209    2789

  Fragments arranged by size: 

                2789     459     209     133
 
TatI w'GTAC_w

Cuts at:   3105    3105 
   Size:       3590
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    127     159    3107    3139     127 
   Size:         32    2948      32     578

  Fragments arranged by size: 

                2948     578      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1387    1387 
   Size:       3590
 
XbaI T'CTAG_A

Cuts at:    621     621 
   Size:       3590
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    415    1085     415 
   Size:        670    2920
 
XmaI C'CCGG_G

Cuts at:    610     610 
   Size:       3590
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PmlI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI     BmtI
     BpmI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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