Restriction map for pF1KE0134
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DraI        
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MfeI        
MluI        MroI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tru9I       Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AgeI           26  ACCGGT               A^CCGGT
AlwI          492  GATCC                GGATC(4/5)
ApaI          629  GGGCCC               GGGCC^C
AviII         455  TGCGCA               TGC^GCA
BanI          349  GGTGCC               G^G[CT][GA]CC
BanII         629  GGGCCC               G[GA]GC[CT]^C
BbsI          544  GAAGAC               GAAGAC(2/6)
BcnI          261  CCGGG                CC^[GC]GG
BglI          190  GCCCTGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          193  CTGGAG               CTGGAG(16/14)
BpuAI         544  GAAGAC               GAAGAC(2/6)
BsaBI         492  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI          26  ACCGGT               [AT]^CCGG[AT]
BsgI          634  CTGCAC               GTGCAG(16/14)
BsmAI          57  GTCTC                GTCTC(1/5)
BsmFI          76  GTCCC                GGGAC(10/14)
BsrFI          26  ACCGGT               [GA]^CCGG[CT]
BsrI          602  CCAGT                ACTGG(1/-1)
BstYI         491  AGATCC               [GA]^GATC[CT]
CfoI          456  GCGC                 GCG^C
Cfr10I         26  ACCGGT               [GA]^CCGG[CT]
DpnII         492  GATC                 ^GATC
DraIII        151  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
FspI          455  TGCGCA               TGC^GCA
HhaI          456  GCGC                 GCG^C
HinP1I        456  GCGC                 G^CGC
HinfI          90  GATTC                G^A[ACGT]TC
HphI          518  TCACC                GGTGA(8/7)
MaeII         284  ACGT                 A^CGT
MaeIII         23  GTTAC                ^GT[ACGT]AC
MamI          492  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          492  GATC                 ^GATC
MflI          491  AGATCC               [GA]^GATC[CT]
MspA1I        175  CAGCTG               C[AC]G^C[GT]G
NciI          261  CCGGG                CC^[GC]GG
NdeII         492  GATC                 ^GATC
PinAI          26  ACCGGT               A^CCGGT
PstI          424  CTGCAG               CTGCA^G
PvuII         175  CAGCTG               CAG^CTG
Sau3AI        492  GATC                 ^GATC
SfcI          424  CTGCAG               C^T[GA][CT]AG
SphI          341  GCATGC               GCATG^C
TfiI           90  GATTC                G^A[AT]TC
TspRI         372  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          215  CCAATAAGTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         491  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI          206  GAATTT               [GA]^AATT[CT]
              538  GAATTT              
AluI          176  AGCT                 AG^CT
              422  AGCT                
ApoI          206  GAATTT               [GA]^AATT[CT]
              538  GAATTT              
BmyI          369  GGGCAC               G[AGT]GC[ACT]^C
              629  GGGCCC              
Bsp1286I      369  GGGCAC               G[AGT]GC[ACT]^C
              629  GGGCCC              
Cac8I         341  GCATGC               GC[ACGT]^[ACGT]GC
              627  GCGGGC              
DraII         449  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              629  GGGCCCT             
EcoO109I      449  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              629  GGGCCCT             
FauI           13  CCCGC                CCCGC(4/6)
              627  GCGGG               
HapII          27  CCGG                 C^CGG
              261  CCGG                
HpaII          27  CCGG                 C^CGG
              261  CCGG                
MboII         489  GAAGA                GAAGA(8/7)
              544  GAAGA               
MspI           27  CCGG                 C^CGG
              261  CCGG                
NspI          341  GCATGC               [GA]CATG^[CT]
              438  GCATGT              
SfaNI          82  GATGC                GCATC(5/9)
              293  GCATC               
Tsp509I       207  AATT                 ^AATT
              539  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AfaI        BalI        BbvI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cfr13I      EaeI        EcoRII      Fnu4HI      FokI        
HaeIII      ItaI        MluNI       MnlI        MscI        
MvaI        MwoI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       TseI        
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