Restriction map for pF1KE0134
 (Linear) (Six-base) MAPSORT of: KIEE0134.seq  Check: 5231  from: 1  to: 642



KIEE0134.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     642 
   Size:        206     436
 
AgeI A'CCGG_T

Cuts at:      0      26     642 
   Size:         26     616
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     439     642 
   Size:        439     203
 
ApaI G_GGCC'C

Cuts at:      0     633     642 
   Size:        633       9
 
ApoI r'AATT_y

Cuts at:      0     206     538     642 
   Size:        206     332     104

  Fragments arranged by size: 

                 332     206     104
 
BanI G'GyrC_C

Cuts at:      0     349     642 
   Size:        349     293
 
BanII G_rGCy'C

Cuts at:      0     633     642 
   Size:        633       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     551     642 
   Size:        551      91
 
BglI GCCn_nnn'nGGC

Cuts at:      0     196     642 
   Size:        196     446
 
Bme1580I G_kGCm'C

Cuts at:      0     373     633     642 
   Size:        373     260       9

  Fragments arranged by size: 

                 373     260       9
 
BmgBI CAC'GTC

Cuts at:      0     285     642 
   Size:        285     357
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     596     642 
   Size:        596      46
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     642 
   Size:        214     428
 
BsaBI GATnn'nnATC

Cuts at:      0     496     642 
   Size:        496     146
 
BsaWI w'CCGG_w

Cuts at:      0      26     642 
   Size:         26     616
 
BseYI C'CCAG_C

Cuts at:      0     316     642 
   Size:        316     326
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     642 
   Size:        619      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     373     633     642 
   Size:        373     260       9

  Fragments arranged by size: 

                 373     260       9
 
BsrFI r'CCGG_y

Cuts at:      0      26     642 
   Size:         26     616
 
BstYI r'GATC_y

Cuts at:      0     491     642 
   Size:        491     151
 
DraIII CAC_nnn'GTG

Cuts at:      0     156     642 
   Size:        156     486
 
EaeI y'GGCC_r

Cuts at:      0     147     377     393     480     642 
   Size:        147     230      16      87     162

  Fragments arranged by size: 

                 230     162     147      87      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     126     642 
   Size:        126     516
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     214     642 
   Size:        206       8     428

  Fragments arranged by size: 

                 428     206       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     450     630     642 
   Size:        450     180      12

  Fragments arranged by size: 

                 450     180      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      78     531     642 
   Size:         78     453     111

  Fragments arranged by size: 

                 453     111      78
 
FspI TGC'GCA

Cuts at:      0     457     642 
   Size:        457     185
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     163     195     642 
   Size:        163      32     447

  Fragments arranged by size: 

                 447     163      32
 
MscI TGG'CCA

Cuts at:      0     149     379     395     642 
   Size:        149     230      16     247

  Fragments arranged by size: 

                 247     230     149      16
 
MspA1I CmG'CkG

Cuts at:      0     177     642 
   Size:        177     465
 
NspI r_CATG'y

Cuts at:      0     345     442     642 
   Size:        345      97     200

  Fragments arranged by size: 

                 345     200      97
 
PasI CC'CwG_GG

Cuts at:      0     366     642 
   Size:        366     276
 
PspOMI G'GGCC_C

Cuts at:      0     629     642 
   Size:        629      13
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      31      63     642 
   Size:         31      32     579

  Fragments arranged by size: 

                 579      32      31
 
PstI C_TGCA'G

Cuts at:      0     428     642 
   Size:        428     214
 
PvuII CAG'CTG

Cuts at:      0     177     642 
   Size:        177     465
 
SfcI C'TryA_G

Cuts at:      0     424     642 
   Size:        424     218
 
SphI G_CATG'C

Cuts at:      0     345     642 
   Size:        345     297
 
TatI w'GTAC_w

Cuts at:      0     221     593     642 
   Size:        221     372      49

  Fragments arranged by size: 

                 372     221      49
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     222     642 
   Size:        222     420
 

 Enzymes that do cut:

     AcuI     AgeI     AlfI     ApaI     ApoI     BanI    BanII     BbsI
     BglI Bme1580I    BmgBI     BmrI     BpmI    BsaBI    BsaWI    BseYI
     BsgI Bsp1286I    BsrFI    BstYI   DraIII     EaeI     EciI  Eco57MI
 EcoO109I     FalI     FspI    Hin4I     MscI   MspA1I     NspI     PasI
   PspOMI     PsrI     PstI    PvuII     SfcI     SphI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI
     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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